1lcy - Revision history

OCA at 07:32, 14 February 2024

OCA — Wed, 14 Feb 2024 07:32:05 GMT

←Older revision		Revision as of 07:32, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1lcy' size='340' side='right'caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1lcy' size='340' side='right'caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LCY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [https://pdbe.org/1lcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [https://www.ebi.ac.uk/pdbsum/1lcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lcy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [https://pdbe.org/1lcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [https://www.ebi.ac.uk/pdbsum/1lcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lcy ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:[https://omim.org/entry/610297 610297]]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.<ref>PMID:15961413</ref> <ref>PMID:18401856</ref>	+	[https://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN] Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:[https://omim.org/entry/610297 610297]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.<ref>PMID:15961413</ref> <ref>PMID:18401856</ref>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.<ref>PMID:15200957</ref> <ref>PMID:19502560</ref>	+	[https://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN] Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.<ref>PMID:15200957</ref> <ref>PMID:19502560</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 21:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lcy ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lcy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	HtrA2/Omi, a mitochondrial serine protease in mammals, is important in programmed cell death. However, the underlining mechanism of HtrA2/Omi-mediated apoptosis remains unclear. Analogous to the bacterial homolog HtrA (DegP), the mature HtrA2 protein contains a central serine protease domain and a C-terminal PDZ domain. The 2.0 A crystal structure of HtrA2/Omi reveals the formation of a pyramid-shaped homotrimer mediated exclusively by the serine protease domains. The peptide-binding pocket of the PDZ domain is buried in the intimate interface between the PDZ and the protease domains. Mutational analysis reveals that the monomeric HtrA2/Omi mutants are unable to induce cell death and are deficient in protease activity. The PDZ domain modulates HtrA2/Omi-mediated cell death activity by regulating its serine protease activity. These structural and biochemical observations provide an important framework for deciphering the mechanisms of HtrA2/Omi-mediated apoptosis.
-
-	Structural insights into the pro-apoptotic function of mitochondrial serine protease HtrA2/Omi.,Li W, Srinivasula SM, Chai J, Li P, Wu JW, Zhang Z, Alnemri ES, Shi Y Nat Struct Biol. 2002 Jun;9(6):436-41. PMID:11967569<ref>PMID:11967569</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1lcy" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Proteinase\|Proteinase]]
	*[[Proteinase 3D structures\|Proteinase 3D structures]]		*[[Proteinase 3D structures\|Proteinase 3D structures]]
	== References ==		== References ==
Line 37:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alnemri~~, E S~~]]	+	[[Category: Alnemri ES]]
-	[[Category: Chai, J]]	+	[[Category: Chai J]]
-	[[Category: Li, P]]	+	[[Category: Li P]]
-	[[Category: Li, W]]	+	[[Category: Li W]]
-	[[Category: Shi, Y]]	+	[[Category: Shi Y]]
-	[[Category: Srinivasula~~, S M~~]]	+	[[Category: Srinivasula SM]]
-	[[Category: Wu~~, J W~~]]	+	[[Category: Wu JW]]
-	[[Category: Zhang, Z]]	+	[[Category: Zhang Z]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Caspase activation]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Iap-binding]]~~	+
-	~~[[Category: Pdz domain]]~~	+
-	~~[[Category: Serine protease~~]]	+

OCA at 05:53, 28 April 2021

OCA — Wed, 28 Apr 2021 05:53:29 GMT

←Older revision		Revision as of 05:53, 28 April 2021
Line 3:		Line 3:
	<StructureSection load='1lcy' size='340' side='right'caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1lcy' size='340' side='right'caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1LCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LCY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [~~http~~://pdbe.org/1lcy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1lcy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1lcy ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [https://pdbe.org/1lcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [https://www.ebi.ac.uk/pdbsum/1lcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lcy ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:[~~http~~://omim.org/entry/610297 610297]]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.<ref>PMID:15961413</ref> <ref>PMID:18401856</ref>	+	[[https://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:[https://omim.org/entry/610297 610297]]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.<ref>PMID:15961413</ref> <ref>PMID:18401856</ref>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.<ref>PMID:15200957</ref> <ref>PMID:19502560</ref>	+	[[https://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.<ref>PMID:15200957</ref> <ref>PMID:19502560</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	==See Also==		==See Also==
	*[[Proteinase\|Proteinase]]		*[[Proteinase\|Proteinase]]
		+	*[[Proteinase 3D structures\|Proteinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:37, 24 July 2019

OCA — Wed, 24 Jul 2019 09:37:14 GMT

←Older revision		Revision as of 09:37, 24 July 2019
Line 1:		Line 1:

	==Crystal Structure of the Mitochondrial Serine Protease HtrA2==		==Crystal Structure of the Mitochondrial Serine Protease HtrA2==
-	<StructureSection load='1lcy' size='340' side='right' caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1lcy' size='340' side='right'caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LCY FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LCY FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lc/1lcy_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lc/1lcy_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1lcy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1lcy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Proteinase\|Proteinase]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Alnemri, E S]]		[[Category: Alnemri, E S]]
	[[Category: Chai, J]]		[[Category: Chai, J]]

OCA at 10:55, 18 October 2017

OCA — Wed, 18 Oct 2017 10:55:21 GMT

←Older revision		Revision as of 10:55, 18 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure of the Mitochondrial Serine Protease HtrA2==		==Crystal Structure of the Mitochondrial Serine Protease HtrA2==
	<StructureSection load='1lcy' size='340' side='right' caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1lcy' size='340' side='right' caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LCY FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LCY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [http://pdbe.org/1lcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [http://www.ebi.ac.uk/pdbsum/1lcy PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [http://pdbe.org/1lcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [http://www.ebi.ac.uk/pdbsum/1lcy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lcy ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lcy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:35, 11 September 2015

OCA — Fri, 11 Sep 2015 09:35:08 GMT

←Older revision		Revision as of 09:35, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1lcy' size='340' side='right' caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1lcy' size='340' side='right' caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LCY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [http://www.ebi.ac.uk/pdbsum/1lcy PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [http://pdbe.org/1lcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [http://www.ebi.ac.uk/pdbsum/1lcy PDBsum]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1lcy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Alnemri, E S]]		[[Category: Alnemri, E S]]
	[[Category: Chai, J]]		[[Category: Chai, J]]

OCA at 16:13, 5 January 2015

OCA — Mon, 05 Jan 2015 16:13:26 GMT

←Older revision		Revision as of 16:13, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LCY FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LCY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [http://www.ebi.ac.uk/pdbsum/1lcy PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [http://www.ebi.ac.uk/pdbsum/1lcy PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:[http://omim.org/entry/610297 610297]]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.<ref>PMID:15961413</ref> <ref>PMID:18401856</ref>		[[http://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:[http://omim.org/entry/610297 610297]]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.<ref>PMID:15961413</ref> <ref>PMID:18401856</ref>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Alnemri, E S.]]	+	[[Category: Alnemri, E S]]
-	[[Category: Chai, J.]]	+	[[Category: Chai, J]]
-	[[Category: Li, P.]]	+	[[Category: Li, P]]
-	[[Category: Li, W.]]	+	[[Category: Li, W]]
-	[[Category: Shi, Y.]]	+	[[Category: Shi, Y]]
-	[[Category: Srinivasula, S M.]]	+	[[Category: Srinivasula, S M]]
-	[[Category: Wu, J W.]]	+	[[Category: Wu, J W]]
-	[[Category: Zhang, Z.]]	+	[[Category: Zhang, Z]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Caspase activation]]		[[Category: Caspase activation]]

OCA at 17:12, 29 September 2014

OCA — Mon, 29 Sep 2014 17:12:28 GMT

←Older revision		Revision as of 17:12, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1lcy~~\| ~~PDB~~=1lcy ~~\| SCENE~~= }}	+	==Crystal Structure of the Mitochondrial Serine Protease HtrA2==
-	===~~Crystal Structure~~ of the ~~Mitochondrial Serine Protease HtrA2~~===	+	<StructureSection load='1lcy' size='340' side='right' caption='[[1lcy]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_11967569}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LCY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lcy RCSB], [http://www.ebi.ac.uk/pdbsum/1lcy PDBsum]</span></td></tr>
		+	<table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:[http://omim.org/entry/610297 610297]]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.<ref>PMID:15961413</ref> <ref>PMID:18401856</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.<ref>PMID:15200957</ref> <ref>PMID:19502560</ref>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lc/1lcy_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	HtrA2/Omi, a mitochondrial serine protease in mammals, is important in programmed cell death. However, the underlining mechanism of HtrA2/Omi-mediated apoptosis remains unclear. Analogous to the bacterial homolog HtrA (DegP), the mature HtrA2 protein contains a central serine protease domain and a C-terminal PDZ domain. The 2.0 A crystal structure of HtrA2/Omi reveals the formation of a pyramid-shaped homotrimer mediated exclusively by the serine protease domains. The peptide-binding pocket of the PDZ domain is buried in the intimate interface between the PDZ and the protease domains. Mutational analysis reveals that the monomeric HtrA2/Omi mutants are unable to induce cell death and are deficient in protease activity. The PDZ domain modulates HtrA2/Omi-mediated cell death activity by regulating its serine protease activity. These structural and biochemical observations provide an important framework for deciphering the mechanisms of HtrA2/Omi-mediated apoptosis.

-	~~==Disease==~~	+	Structural insights into the pro-apoptotic function of mitochondrial serine protease HtrA2/Omi.,Li W, Srinivasula SM, Chai J, Li P, Wu JW, Zhang Z, Alnemri ES, Shi Y Nat Struct Biol. 2002 Jun;9(6):436-41. PMID:11967569<ref>PMID:11967569</ref>
-	~~[[http://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Defects in HTRA2 are~~ the ~~cause~~ of ~~Parkinson disease type 13 (PARK13) [MIM:[http:~~/~~/omim.org/entry/610297 610297]]~~. ~~A complex neurodegenerative disorder characterized by bradykinesia~~, ~~resting tremor~~, ~~muscular rigidity and postural instability~~, ~~as well as by a clinically significant response to treatment with levodopa~~. ~~The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies~~ (~~intraneuronal accumulations of aggregated proteins~~)~~, in surviving neurons in various areas of the brain~~.~~<ref>~~PMID:~~15961413</ref>~~<ref>PMID:~~18401856~~</ref>	+

-	~~==Function==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[http:~~/~~/www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Serine protease that shows proteolytic activity against~~ a ~~non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition~~ of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. ~~Cleaves THAP5 and promotes its degradation during apoptosis~~. ~~Isoform 2 seems to be proteolytically inactive~~.<~~ref~~>~~PMID:15200957~~</~~ref~~>~~<ref>PMID:19502560</ref>~~	+	</div>
-		+	== References ==
-	==~~About this Structure==~~	+	<references/>
-	[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA].	+	__TOC__
-		+	</StructureSection>
-	~~==Reference~~==	+
-	~~<ref group="xtra">PMID:011967569</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Alnemri, E S.]]		[[Category: Alnemri, E S.]]

OCA at 06:17, 25 March 2013

OCA — Mon, 25 Mar 2013 06:17:51 GMT

←Older revision		Revision as of 06:17, 25 March 2013
Line 1:		Line 1:
-	[[Image:1lcy.png\|left\|200px]]
-
	{{STRUCTURE_1lcy\| PDB=1lcy \| SCENE= }}		{{STRUCTURE_1lcy\| PDB=1lcy \| SCENE= }}
-
	===Crystal Structure of the Mitochondrial Serine Protease HtrA2===		===Crystal Structure of the Mitochondrial Serine Protease HtrA2===
		+	{{ABSTRACT_PUBMED_11967569}}

-	~~{{ABSTRACT_PUBMED_11967569}}~~	+	==Disease==
		+	[[http://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:[http://omim.org/entry/610297 610297]]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.<ref>PMID:15961413</ref><ref>PMID:18401856</ref>
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/HTRA2_HUMAN HTRA2_HUMAN]] Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.<ref>PMID:15200957</ref><ref>PMID:19502560</ref>

	==About this Structure==		==About this Structure==
Line 11:		Line 13:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:011967569</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011967569</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Alnemri, E S.]]		[[Category: Alnemri, E S.]]

OCA: Protected "1lcy" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 20:26:53 GMT

Protected "1lcy" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:26, 5 December 2012

OCA at 20:26, 5 December 2012

OCA — Wed, 05 Dec 2012 20:26:50 GMT

←Older revision		Revision as of 20:26, 5 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1lcy.png\|left\|200px]]		[[Image:1lcy.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1lcy", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1lcy\| PDB=1lcy \| SCENE= }}		{{STRUCTURE_1lcy\| PDB=1lcy \| SCENE= }}

	===Crystal Structure of the Mitochondrial Serine Protease HtrA2===		===Crystal Structure of the Mitochondrial Serine Protease HtrA2===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11967569}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11967569 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11967569}}		{{ABSTRACT_PUBMED_11967569}}

	==About this Structure==		==About this Structure==
-	~~1LCY~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA].	+	[[1lcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCY OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11967569~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011967569</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Alnemri, E S.]]		[[Category: Alnemri, E S.]]
Line 35:		Line 23:
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Caspase activation]]		[[Category: Caspase activation]]
		+	[[Category: Hydrolase]]
	[[Category: Iap-binding]]		[[Category: Iap-binding]]
	[[Category: Pdz domain]]		[[Category: Pdz domain]]
	[[Category: Serine protease]]		[[Category: Serine protease]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:50:15 2009''