1ldt - Revision history

OCA at 06:06, 3 April 2024

OCA — Wed, 03 Apr 2024 06:06:11 GMT

←Older revision		Revision as of 06:06, 3 April 2024
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	<StructureSection load='1ldt' size='340' side='right'caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ldt' size='340' side='right'caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Hirme Hirme~~] and [https://en.wikipedia.org/wiki/~~Pig Pig~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LDT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LDT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [https://pdbe.org/1ldt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [https://www.ebi.ac.uk/pdbsum/1ldt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ldt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [https://pdbe.org/1ldt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [https://www.ebi.ac.uk/pdbsum/1ldt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ldt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~LDTI_HIRME LDTI_HIRME~~]~~] Acts as an inhibitor of human tryptase, trypsin and chymotrypsin. Probably acts to block host defense mechanisms.~~	+	[https://www.uniprot.org/uniprot/TRYP_PIG TRYP_PIG]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ldt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ldt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The x-ray crystal structure of recombinant leech-derived tryptase inhibitor (rLDTI) has been solved to a resolution of 1.9 A in complex with porcine trypsin. The nonclassical Kazal-type inhibitor exhibits the same overall architecture as that observed in solution and in rhodniin. The complex reveals structural aspects of the mast cell proteinase tryptase. The conformation of the binding region of rLDTI suggests that tryptase has a restricted active site cleft. The basic amino terminus of rLDTI, apparently flexible from previous NMR measurements, approaches the 148-loop of trypsin. This loop has an acidic equivalent in tryptase, suggesting that the basic amino terminus could make favorable electrostatic interactions with the tryptase molecule. A series of rLDTI variants constructed to probe this hypothesis confirmed that the amino-terminal Lys-Lys sequence plays a role in inhibition of human lung tryptase but not of trypsin or chymotrypsin. The location of such an acidic surface patch is in accordance with the known low molecular weight inhibitors of tryptase.
-
-	The three-dimensional structure of recombinant leech-derived tryptase inhibitor in complex with trypsin. Implications for the structure of human mast cell tryptase and its inhibition.,Stubbs MT, Morenweiser R, Sturzebecher J, Bauer M, Bode W, Huber R, Piechottka GP, Matschiner G, Sommerhoff CP, Fritz H, Auerswald EA J Biol Chem. 1997 Aug 8;272(32):19931-7. PMID:9242660<ref>PMID:9242660</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ldt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Trypsin 3D structures\|Trypsin 3D structures]]		*[[Trypsin 3D structures\|Trypsin 3D structures]]
	*[[Tryptase inhibitor\|Tryptase inhibitor]]		*[[Tryptase inhibitor\|Tryptase inhibitor]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hirme~~]]	+	[[Category: Hirudo medicinalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pig]]~~	+	[[Category: Sus scrofa]]
-	~~[[Category: Trypsin~~]]	+	[[Category: Stubbs MT]]
-	[[Category: Stubbs~~, M T]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Inflammation]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Tryptase~~]]	+

OCA at 05:53, 28 April 2021

OCA — Wed, 28 Apr 2021 05:53:43 GMT

←Older revision		Revision as of 05:53, 28 April 2021
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	<StructureSection load='1ldt' size='340' side='right'caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ldt' size='340' side='right'caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Hirme Hirme] and [~~http~~://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1LDT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hirme Hirme] and [https://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LDT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [~~http~~://pdbe.org/1ldt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ldt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ldt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [https://pdbe.org/1ldt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [https://www.ebi.ac.uk/pdbsum/1ldt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ldt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LDTI_HIRME LDTI_HIRME]] Acts as an inhibitor of human tryptase, trypsin and chymotrypsin. Probably acts to block host defense mechanisms.	+	[[https://www.uniprot.org/uniprot/LDTI_HIRME LDTI_HIRME]] Acts as an inhibitor of human tryptase, trypsin and chymotrypsin. Probably acts to block host defense mechanisms.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Trypsin\|Trypsin]]	+	*[[Trypsin 3D structures\|Trypsin 3D structures]]
	*[[Tryptase inhibitor\|Tryptase inhibitor]]		*[[Tryptase inhibitor\|Tryptase inhibitor]]
	== References ==		== References ==

OCA at 09:06, 24 July 2019

OCA — Wed, 24 Jul 2019 09:06:57 GMT

←Older revision		Revision as of 09:06, 24 July 2019
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	==COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN==		==COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN==
-	<StructureSection load='1ldt' size='340' side='right' caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1ldt' size='340' side='right'caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hirme Hirme] and [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LDT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hirme Hirme] and [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LDT FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ld/1ldt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ld/1ldt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:

	==See Also==		==See Also==
		+	*[[Trypsin\|Trypsin]]
	*[[Tryptase inhibitor\|Tryptase inhibitor]]		*[[Tryptase inhibitor\|Tryptase inhibitor]]
	== References ==		== References ==
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	</StructureSection>		</StructureSection>
	[[Category: Hirme]]		[[Category: Hirme]]
		+	[[Category: Large Structures]]
	[[Category: Pig]]		[[Category: Pig]]
	[[Category: Trypsin]]		[[Category: Trypsin]]

OCA at 10:43, 18 October 2017

OCA — Wed, 18 Oct 2017 10:43:29 GMT

←Older revision		Revision as of 10:43, 18 October 2017
Line 1:		Line 1:
		+
	==COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN==		==COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN==
	<StructureSection load='1ldt' size='340' side='right' caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ldt' size='340' side='right' caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hirme Hirme] and [http://en.wikipedia.org/wiki/~~Sus_scrofa Sus scrofa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LDT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hirme Hirme] and [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LDT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [http://pdbe.org/1ldt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [http://www.ebi.ac.uk/pdbsum/1ldt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [http://pdbe.org/1ldt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [http://www.ebi.ac.uk/pdbsum/1ldt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ldt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ldt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 30:		Line 31:

	==See Also==		==See Also==
-	*[[Trypsin\|Trypsin]]
	*[[Tryptase inhibitor\|Tryptase inhibitor]]		*[[Tryptase inhibitor\|Tryptase inhibitor]]
	== References ==		== References ==
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Hirme]]		[[Category: Hirme]]
-	[[Category: ~~Sus scrofa~~]]	+	[[Category: Pig]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Stubbs, M T]]		[[Category: Stubbs, M T]]

OCA at 17:29, 11 September 2015

OCA — Fri, 11 Sep 2015 17:29:30 GMT

←Older revision		Revision as of 17:29, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ldt' size='340' side='right' caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1ldt' size='340' side='right' caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Hirudo_medicinalis Hirudo medicinalis~~] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LDT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hirme Hirme] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LDT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [http://www.ebi.ac.uk/pdbsum/1ldt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [http://pdbe.org/1ldt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [http://www.ebi.ac.uk/pdbsum/1ldt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ldt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hirudo medicinalis~~]]	+	[[Category: Hirme]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Trypsin]]		[[Category: Trypsin]]

OCA at 19:37, 25 December 2014

OCA — Thu, 25 Dec 2014 19:37:49 GMT

←Older revision		Revision as of 19:37, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LDT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LDT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [http://www.ebi.ac.uk/pdbsum/1ldt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [http://www.ebi.ac.uk/pdbsum/1ldt PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LDTI_HIRME LDTI_HIRME]] Acts as an inhibitor of human tryptase, trypsin and chymotrypsin. Probably acts to block host defense mechanisms.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
-	[[Category: Stubbs, M T.]]	+	[[Category: Stubbs, M T]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Inflammation]]		[[Category: Inflammation]]
	[[Category: Inhibitor]]		[[Category: Inhibitor]]
	[[Category: Tryptase]]		[[Category: Tryptase]]

OCA at 14:30, 29 September 2014

OCA — Mon, 29 Sep 2014 14:30:25 GMT

←Older revision		Revision as of 14:30, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1ldt\| PDB=1ldt \| SCENE= }}~~	+	==COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN==
-	===COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN===	+	<StructureSection load='1ldt' size='340' side='right' caption='[[1ldt]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_9242660}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ldt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LDT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB], [http://www.ebi.ac.uk/pdbsum/1ldt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ld/1ldt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The x-ray crystal structure of recombinant leech-derived tryptase inhibitor (rLDTI) has been solved to a resolution of 1.9 A in complex with porcine trypsin. The nonclassical Kazal-type inhibitor exhibits the same overall architecture as that observed in solution and in rhodniin. The complex reveals structural aspects of the mast cell proteinase tryptase. The conformation of the binding region of rLDTI suggests that tryptase has a restricted active site cleft. The basic amino terminus of rLDTI, apparently flexible from previous NMR measurements, approaches the 148-loop of trypsin. This loop has an acidic equivalent in tryptase, suggesting that the basic amino terminus could make favorable electrostatic interactions with the tryptase molecule. A series of rLDTI variants constructed to probe this hypothesis confirmed that the amino-terminal Lys-Lys sequence plays a role in inhibition of human lung tryptase but not of trypsin or chymotrypsin. The location of such an acidic surface patch is in accordance with the known low molecular weight inhibitors of tryptase.

-	~~==About this Structure==~~	+	The three-dimensional structure of recombinant leech-derived tryptase inhibitor in complex with trypsin. Implications for the structure of human mast cell tryptase and its inhibition.,Stubbs MT, Morenweiser R, Sturzebecher J, Bauer M, Bode W, Huber R, Piechottka GP, Matschiner G, Sommerhoff CP, Fritz H, Auerswald EA J Biol Chem. 1997 Aug 8;272(32):19931-7. PMID:9242660<ref>PMID:9242660</ref>
-	~~[[1ldt]] is a 2 chain~~ structure with ~~sequence from [http://en~~.~~wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis]~~ and ~~[http://en~~.~~wikipedia~~.~~org/wiki/Sus_scrofa Sus scrofa]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1LDT OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Trypsin\|Trypsin]]		*[[Trypsin\|Trypsin]]
	*[[Tryptase inhibitor\|Tryptase inhibitor]]		*[[Tryptase inhibitor\|Tryptase inhibitor]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009242660<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Hirudo medicinalis]]		[[Category: Hirudo medicinalis]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]

OCA at 09:48, 11 March 2013

OCA — Mon, 11 Mar 2013 09:48:09 GMT

←Older revision		Revision as of 09:48, 11 March 2013
Line 1:		Line 1:
-	[[Image:1ldt.png\|left\|200px]]
-
	{{STRUCTURE_1ldt\| PDB=1ldt \| SCENE= }}		{{STRUCTURE_1ldt\| PDB=1ldt \| SCENE= }}
-
	===COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN===		===COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN===
-
	{{ABSTRACT_PUBMED_9242660}}		{{ABSTRACT_PUBMED_9242660}}

OCA at 20:37, 20 October 2012

OCA — Sat, 20 Oct 2012 20:37:54 GMT

←Older revision		Revision as of 20:37, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1ldt]] is a 2 chain structure ~~of [[Trypsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA].	+	[[1ldt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDT OCA].

	==See Also==		==See Also==
	*[[Trypsin\|Trypsin]]		*[[Trypsin\|Trypsin]]
		+	*[[Tryptase inhibitor\|Tryptase inhibitor]]

	==Reference==		==Reference==

OCA: Protected "1ldt" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 11:06:30 GMT

Protected "1ldt" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:06, 27 July 2012