1lel - Revision history

OCA at 08:08, 3 April 2024

OCA — Wed, 03 Apr 2024 08:08:49 GMT

←Older revision		Revision as of 08:08, 3 April 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LEL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LEL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BH7:E~~-~~AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>~~, ~~<scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1lcv\|1lcv]]~~, ~~[[1lcw\|1lcw]], [[1lcz\|1lcz]], [[1ldo\|1ldo]], [[1ldq\|1ldq]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BH7:E-AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [https://pdbe.org/1lel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [https://www.ebi.ac.uk/pdbsum/1lel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lel ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [https://pdbe.org/1lel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [https://www.ebi.ac.uk/pdbsum/1lel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lel ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK]] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).	+	[https://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lel ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lel ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We have studied the structural elements that affect ligand exchange between the two high affinity biotin-binding proteins, egg white avidin and its bacterial analogue, streptavidin. For this purpose, we have developed a simple assay based on the antipodal behavior of the two proteins toward hydrolysis of biotinyl p-nitrophenyl ester (BNP). The assay provided the experimental basis for these studies. It was found that biotin migrates unidirectionally from streptavidin to avidin. Conversely, the biotin derivative, BNP, is transferred in the opposite direction, from avidin to streptavidin. A previous crystallographic study (Huberman, T., Eisenberg-Domovich, Y., Gitlin, G., Kulik, T., Bayer, E. A., Wilchek, M., and Livnah, O. (2001) J. Biol. Chem. 276, 32031-32039) provided insight into a plausible explanation for these results. These data revealed that the non-hydrolyzable BNP analogue, biotinyl p-nitroanilide, was almost completely sheltered in streptavidin as opposed to avidin in which the disordered conformation of a critical loop resulted in the loss of several hydrogen bonds and concomitant exposure of the analogue to the solvent. In order to determine the minimal modification of the biotin molecule required to cause the disordered loop conformation, the structures of avidin and streptavidin were determined with norbiotin, homobiotin, and a common long-chain biotin derivative, biotinyl epsilon-aminocaproic acid. Six new crystal structures of the avidin and streptavidin complexes with the latter biotin analogues and derivatives were thus elucidated. It was found that extending the biotin side chain by a single CH(2) group (i.e. homobiotin) is sufficient to result in this remarkable conformational change in the loop of avidin. These results bear significant biotechnological importance, suggesting that complexes containing biotinylated probes with streptavidin would be more stable than those with avidin. These findings should be heeded when developing new drugs based on lead compounds because it is difficult to predict the structural and conformational consequences on the resultant protein-ligand interactions.
-
-	Ligand exchange between proteins. Exchange of biotin and biotin derivatives between avidin and streptavidin.,Pazy Y, Kulik T, Bayer EA, Wilchek M, Livnah O J Biol Chem. 2002 Aug 23;277(34):30892-900. Epub 2002 Jun 7. PMID:12055191<ref>PMID:12055191</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1lel" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bayer~~, E A~~]]	+	[[Category: Bayer EA]]
-	[[Category: Kulik, T]]	+	[[Category: Kulik T]]
-	[[Category: Livnah, O]]	+	[[Category: Livnah O]]
-	[[Category: Pazy, Y]]	+	[[Category: Pazy Y]]
-	[[Category: Wilchek, M]]	+	[[Category: Wilchek M]]
-	~~[[Category: Avidin]]~~	+
-	~~[[Category: Biotin]]~~	+
-	~~[[Category: Ligand exchange]]~~	+
-	~~[[Category: Streptavidin]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 06:43, 18 August 2021

OCA — Wed, 18 Aug 2021 06:43:21 GMT

←Older revision		Revision as of 06:43, 18 August 2021
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	<StructureSection load='1lel' size='340' side='right'caption='[[1lel]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1lel' size='340' side='right'caption='[[1lel]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1LEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LEL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH7:E-AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>, <scene name='pdbligand=~~NDG~~:2-~~(ACETYLAMINO)-2-DEOXY-A~~-D-~~GLUCOPYRANOSE~~'>~~NDG~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BH7:E-AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lcv\|1lcv]], [[1lcw\|1lcw]], [[1lcz\|1lcz]], [[1ldo\|1ldo]], [[1ldq\|1ldq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1lcv\|1lcv]], [[1lcw\|1lcw]], [[1lcz\|1lcz]], [[1ldo\|1ldo]], [[1ldq\|1ldq]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [~~http~~://pdbe.org/1lel PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1lel PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1lel ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [https://pdbe.org/1lel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [https://www.ebi.ac.uk/pdbsum/1lel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lel ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK]] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).	+	[[https://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK]] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:04, 13 November 2019

OCA — Wed, 13 Nov 2019 12:04:32 GMT

←Older revision		Revision as of 12:04, 13 November 2019
Line 1:		Line 1:

	==The avidin BCAP complex==		==The avidin BCAP complex==
-	<StructureSection load='1lel' size='340' side='right' caption='[[1lel]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='1lel' size='340' side='right'caption='[[1lel]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LEL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LEL FirstGlance]. <br>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1lel" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1lel" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
	[[Category: Bayer, E A]]		[[Category: Bayer, E A]]
	[[Category: Kulik, T]]		[[Category: Kulik, T]]

OCA at 21:14, 24 January 2018

OCA — Wed, 24 Jan 2018 21:14:38 GMT

←Older revision		Revision as of 21:14, 24 January 2018
Line 1:		Line 1:
		+
	==The avidin BCAP complex==		==The avidin BCAP complex==
	<StructureSection load='1lel' size='340' side='right' caption='[[1lel]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1lel' size='340' side='right' caption='[[1lel]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chick Chick~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LEL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH7:E-AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH7:E-AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lcv\|1lcv]], [[1lcw\|1lcw]], [[1lcz\|1lcz]], [[1ldo\|1ldo]], [[1ldq\|1ldq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lcv\|1lcv]], [[1lcw\|1lcw]], [[1lcz\|1lcz]], [[1ldo\|1ldo]], [[1ldq\|1ldq]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [http://pdbe.org/1lel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [http://www.ebi.ac.uk/pdbsum/1lel PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [http://pdbe.org/1lel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [http://www.ebi.ac.uk/pdbsum/1lel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lel ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/le/1lel_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/le/1lel_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1lel" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1lel" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Avidin\|Avidin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chick~~]]	+	[[Category: Gallus gallus]]
	[[Category: Bayer, E A]]		[[Category: Bayer, E A]]
	[[Category: Kulik, T]]		[[Category: Kulik, T]]

OCA at 08:59, 9 February 2016

OCA — Tue, 09 Feb 2016 08:59:33 GMT

←Older revision		Revision as of 08:59, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lel ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:40, 10 September 2015

OCA — Thu, 10 Sep 2015 20:40:43 GMT

←Older revision		Revision as of 20:40, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1lel' size='340' side='right' caption='[[1lel]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1lel' size='340' side='right' caption='[[1lel]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Gallus_gallus Gallus gallus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LEL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH7:E-AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH7:E-AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lcv\|1lcv]], [[1lcw\|1lcw]], [[1lcz\|1lcz]], [[1ldo\|1ldo]], [[1ldq\|1ldq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lcv\|1lcv]], [[1lcw\|1lcw]], [[1lcz\|1lcz]], [[1ldo\|1ldo]], [[1ldq\|1ldq]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [http://www.ebi.ac.uk/pdbsum/1lel PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [http://pdbe.org/1lel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [http://www.ebi.ac.uk/pdbsum/1lel PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1lel" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Gallus gallus~~]]	+	[[Category: Chick]]
	[[Category: Bayer, E A]]		[[Category: Bayer, E A]]
	[[Category: Kulik, T]]		[[Category: Kulik, T]]

OCA at 05:52, 25 December 2014

OCA — Thu, 25 Dec 2014 05:52:35 GMT

←Older revision		Revision as of 05:52, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LEL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LEL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH7:E-AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH7:E-AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lcv\|1lcv]], [[1lcw\|1lcw]], [[1lcz\|1lcz]], [[1ldo\|1ldo]], [[1ldq\|1ldq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lcv\|1lcv]], [[1lcw\|1lcw]], [[1lcz\|1lcz]], [[1ldo\|1ldo]], [[1ldq\|1ldq]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [http://www.ebi.ac.uk/pdbsum/1lel PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [http://www.ebi.ac.uk/pdbsum/1lel PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK]] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
-	[[Category: Bayer, E A.]]	+	[[Category: Bayer, E A]]
-	[[Category: Kulik, T.]]	+	[[Category: Kulik, T]]
-	[[Category: Livnah, O.]]	+	[[Category: Livnah, O]]
-	[[Category: Pazy, Y.]]	+	[[Category: Pazy, Y]]
-	[[Category: Wilchek, M.]]	+	[[Category: Wilchek, M]]
	[[Category: Avidin]]		[[Category: Avidin]]
	[[Category: Biotin]]		[[Category: Biotin]]

OCA at 16:29, 28 September 2014

OCA — Sun, 28 Sep 2014 16:29:56 GMT

←Older revision		Revision as of 16:29, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1lel.~~png~~\|~~left~~\|200px]]	+	==The avidin BCAP complex==
		+	<StructureSection load='1lel' size='340' side='right' caption='[[1lel]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1lel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LEL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH7:E-AMINO+BIOTINYL+CAPROIC+ACID'>BH7</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lcv\|1lcv]], [[1lcw\|1lcw]], [[1lcz\|1lcz]], [[1ldo\|1ldo]], [[1ldq\|1ldq]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lel OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lel RCSB], [http://www.ebi.ac.uk/pdbsum/1lel PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/le/1lel_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have studied the structural elements that affect ligand exchange between the two high affinity biotin-binding proteins, egg white avidin and its bacterial analogue, streptavidin. For this purpose, we have developed a simple assay based on the antipodal behavior of the two proteins toward hydrolysis of biotinyl p-nitrophenyl ester (BNP). The assay provided the experimental basis for these studies. It was found that biotin migrates unidirectionally from streptavidin to avidin. Conversely, the biotin derivative, BNP, is transferred in the opposite direction, from avidin to streptavidin. A previous crystallographic study (Huberman, T., Eisenberg-Domovich, Y., Gitlin, G., Kulik, T., Bayer, E. A., Wilchek, M., and Livnah, O. (2001) J. Biol. Chem. 276, 32031-32039) provided insight into a plausible explanation for these results. These data revealed that the non-hydrolyzable BNP analogue, biotinyl p-nitroanilide, was almost completely sheltered in streptavidin as opposed to avidin in which the disordered conformation of a critical loop resulted in the loss of several hydrogen bonds and concomitant exposure of the analogue to the solvent. In order to determine the minimal modification of the biotin molecule required to cause the disordered loop conformation, the structures of avidin and streptavidin were determined with norbiotin, homobiotin, and a common long-chain biotin derivative, biotinyl epsilon-aminocaproic acid. Six new crystal structures of the avidin and streptavidin complexes with the latter biotin analogues and derivatives were thus elucidated. It was found that extending the biotin side chain by a single CH(2) group (i.e. homobiotin) is sufficient to result in this remarkable conformational change in the loop of avidin. These results bear significant biotechnological importance, suggesting that complexes containing biotinylated probes with streptavidin would be more stable than those with avidin. These findings should be heeded when developing new drugs based on lead compounds because it is difficult to predict the structural and conformational consequences on the resultant protein-ligand interactions.

-	~~{{STRUCTURE_1lel\| PDB=1lel \| SCENE= }}~~	+	Ligand exchange between proteins. Exchange of biotin and biotin derivatives between avidin and streptavidin.,Pazy Y, Kulik T, Bayer EA, Wilchek M, Livnah O J Biol Chem. 2002 Aug 23;277(34):30892-900. Epub 2002 Jun 7. PMID:12055191<ref>PMID:12055191</ref>

-	~~===The avidin BCAP complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12055191}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1lel]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Gallus_gallus Gallus gallus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA].~~	+

	==See Also==		==See Also==
	*[[Avidin\|Avidin]]		*[[Avidin\|Avidin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012055191<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Bayer, E A.]]		[[Category: Bayer, E A.]]

OCA at 13:20, 20 October 2012

OCA — Sat, 20 Oct 2012 13:20:51 GMT

←Older revision		Revision as of 13:20, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1lel]] is a 2 chain structure ~~of [[Avidin]]~~ with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA].	+	[[1lel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LEL OCA].

	==See Also==		==See Also==

OCA at 11:40, 26 July 2012

OCA — Thu, 26 Jul 2012 11:40:21 GMT

←Older revision		Revision as of 11:40, 26 July 2012
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	[[Image:1lel.png\|left\|200px]]		[[Image:1lel.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
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	{{STRUCTURE_1lel\| PDB=1lel \| SCENE= }}		{{STRUCTURE_1lel\| PDB=1lel \| SCENE= }}

	===The avidin BCAP complex===		===The avidin BCAP complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12055191}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12055191 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_12055191}}		{{ABSTRACT_PUBMED_12055191}}

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	==See Also==		==See Also==
-	*[[Avidin]]	+	*[[Avidin\|Avidin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12055191~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012055191</ref><references group="xtra"/>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Bayer, E A.]]		[[Category: Bayer, E A.]]