1lgc - Revision history

OCA at 08:09, 3 April 2024

OCA — Wed, 03 Apr 2024 08:09:08 GMT

←Older revision		Revision as of 08:09, 3 April 2024
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	<StructureSection load='1lgc' size='340' side='right'caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1lgc' size='340' side='right'caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cyprus-vetch Cyprus-vetch~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LGC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:~~MANGANESE+(II)+ION~~'>MN</scene>, <scene name='pdbligand=~~MPD~~:~~(4S)~~-2-~~METHYL-2,4-PENTANEDIOL~~'>~~MPD~~</scene>, <scene name='pdbligand=~~FUC~~:ALPHA-L-~~FUCOSE~~'>~~FUC~~</scene>, <scene name='pdbligand=~~GAL~~:~~BETA-D-GALACTOSE~~'>~~GAL~~</scene>, <scene name='pdbligand=~~MAN~~:~~ALPHA~~-D-~~MANNOSE~~'>~~MAN~~</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [https://pdbe.org/1lgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [https://www.ebi.ac.uk/pdbsum/1lgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lgc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [https://pdbe.org/1lgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [https://www.ebi.ac.uk/pdbsum/1lgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lgc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LECB_LATOC LECB_LATOC]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lgc ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lgc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: Lectins mediate cell-cell interactions by specifically recognizing oligosaccharide chains. Legume lectins serve as mediators for the symbiotic interactions between plants and nitrogen-fixing microorganisms, an important process in the nitrogen cycle. Lectins from the Viciae tribe have a high affinity for the fucosylated biantennary N-acetyllactosamine-type glycans which are to be found in the majority of N-glycosylproteins. While the structures of several lectins complexed with incomplete oligosaccharides have been solved, no previous structure has included the complete glycoprotein. RESULTS: We have determined the crystal structures of Lathyrus ochrus isolectin II complexed with the N2 monoglycosylated fragment of human lactotransferrin (18 kDa) and an isolated glycopeptide (2.1 kDa) fragment of human lactotransferrin (at 3.3 A and 2.8 A resolution, respectively). Comparison between the two structures showed that the protein part of the glycoprotein has little influence on either the stabilization of the complex or the sugar conformation. In both cases the oligosaccharide adopts the same extended conformation. Besides the essential mannose moiety of the monosaccharide-binding site, the fucose-1' of the core has a large surface of interaction with the lectin. This oligosaccharide conformation differs substantially from that seen in the previously determined isolectin I-octasaccharide complex. Comparison of our structure with that of concanavalin A (ConA) suggests that the ConA binding site cannot accommodate this fucose. CONCLUSIONS: Our results explain the observation that Viciae lectins have a higher affinity for fucosylated oligosaccharides than for unfucosylated ones, whereas the affinity of ConA for these types of oligosaccharides is similar. This explanation is testable by mutagenesis experiments. Our structure shows a large complementary surface area between the oligosaccharide and the lectin, in contrast with the recently determined structure of a complex between the carbohydrate recognition domain of a C-type mammalian lectin and an oligomannoside, where only the non-reducing terminal mannose residue interacts with the lectin.
-
-	Structures of a legume lectin complexed with the human lactotransferrin N2 fragment, and with an isolated biantennary glycopeptide: role of the fucose moiety.,Bourne Y, Mazurier J, Legrand D, Rouge P, Montreuil J, Spik G, Cambillau C Structure. 1994 Mar 15;2(3):209-19. PMID:8069634<ref>PMID:8069634</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1lgc" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cyprus-vetch]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bourne, Y~~]]	+	[[Category: Lathyrus ochrus]]
-	[[Category: ~~Cambillau, C~~]]	+	[[Category: Bourne Y]]
-	[[Category: ~~Lectin~~]]	+	[[Category: Cambillau C]]

OCA at 06:44, 18 August 2021

OCA — Wed, 18 Aug 2021 06:44:10 GMT

←Older revision		Revision as of 06:44, 18 August 2021
Line 3:		Line 3:
	<StructureSection load='1lgc' size='340' side='right'caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1lgc' size='340' side='right'caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Latoc Latoc~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1LGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyprus-vetch Cyprus-vetch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LGC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=~~FUC~~:~~ALPHA-L-FUCOSE~~'>~~FUC~~</scene>, <scene name='pdbligand=~~GAL~~:~~BETA~~-D-~~GALACTOSE~~'>~~GAL~~</scene>, <scene name='pdbligand=~~MAN~~:ALPHA-D-~~MANNOSE~~'>~~MAN~~</scene>, <scene name='pdbligand=MN:~~MANGANESE+(II)+ION~~'>MN</scene>, <scene name='pdbligand=~~MPD~~:~~(4S)~~-2-~~METHYL-2,4-PENTANEDIOL~~'>~~MPD~~</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [~~http~~://pdbe.org/1lgc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1lgc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1lgc ProSAT]</span></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [https://pdbe.org/1lgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [https://www.ebi.ac.uk/pdbsum/1lgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lgc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cyprus-vetch]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Latoc]]
	[[Category: Bourne, Y]]		[[Category: Bourne, Y]]
	[[Category: Cambillau, C]]		[[Category: Cambillau, C]]
	[[Category: Lectin]]		[[Category: Lectin]]

OCA at 12:13, 13 November 2019

OCA — Wed, 13 Nov 2019 12:13:08 GMT

←Older revision		Revision as of 12:13, 13 November 2019
Line 1:		Line 1:

	==INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY==		==INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY==
-	<StructureSection load='1lgc' size='340' side='right' caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='1lgc' size='340' side='right'caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Latoc Latoc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LGC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Latoc Latoc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LGC FirstGlance]. <br>
Line 30:		Line 30:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Latoc]]		[[Category: Latoc]]
	[[Category: Bourne, Y]]		[[Category: Bourne, Y]]
	[[Category: Cambillau, C]]		[[Category: Cambillau, C]]
	[[Category: Lectin]]		[[Category: Lectin]]

OCA at 21:22, 24 January 2018

OCA — Wed, 24 Jan 2018 21:22:47 GMT

←Older revision		Revision as of 21:22, 24 January 2018
Line 1:		Line 1:
		+
	==INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY==		==INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY==
	<StructureSection load='1lgc' size='340' side='right' caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1lgc' size='340' side='right' caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Latoc Latoc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LGC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Latoc Latoc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LGC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:~~MANGANESE+(II)+ION~~'>MN</scene>, <scene name='pdbligand=~~MPD~~:~~(4S)~~-2-~~METHYL-2,4-PENTANEDIOL~~'>~~MPD~~</scene>, <scene name='pdbligand=~~FUC~~:ALPHA-L-~~FUCOSE~~'>~~FUC~~</scene>, <scene name='pdbligand=~~GAL~~:~~BETA-D-GALACTOSE~~'>~~GAL~~</scene>, <scene name='pdbligand=~~MAN~~:~~ALPHA~~-D-~~MANNOSE~~'>~~MAN~~</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [http://pdbe.org/1lgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [http://www.ebi.ac.uk/pdbsum/1lgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lgc ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [http://pdbe.org/1lgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [http://www.ebi.ac.uk/pdbsum/1lgc PDBsum]</span></td></tr>	+
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lg/1lgc_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lg/1lgc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 14:39, 7 February 2016

OCA — Sun, 07 Feb 2016 14:39:52 GMT

←Older revision		Revision as of 14:39, 7 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lgc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:27, 10 September 2015

OCA — Thu, 10 Sep 2015 02:27:45 GMT

←Older revision		Revision as of 02:27, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1lgc' size='340' side='right' caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1lgc' size='340' side='right' caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lathyrus_ochrus Lathyrus ochrus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Latoc Latoc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LGC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [http://www.ebi.ac.uk/pdbsum/1lgc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [http://pdbe.org/1lgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [http://www.ebi.ac.uk/pdbsum/1lgc PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1lgc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lathyrus ochrus~~]]	+	[[Category: Latoc]]
	[[Category: Bourne, Y]]		[[Category: Bourne, Y]]
	[[Category: Cambillau, C]]		[[Category: Cambillau, C]]
	[[Category: Lectin]]		[[Category: Lectin]]

OCA at 15:33, 5 January 2015

OCA — Mon, 05 Jan 2015 15:33:12 GMT

←Older revision		Revision as of 15:33, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LGC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LGC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [http://www.ebi.ac.uk/pdbsum/1lgc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [http://www.ebi.ac.uk/pdbsum/1lgc PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Lathyrus ochrus]]		[[Category: Lathyrus ochrus]]
-	[[Category: Bourne, Y.]]	+	[[Category: Bourne, Y]]
-	[[Category: Cambillau, C.]]	+	[[Category: Cambillau, C]]
	[[Category: Lectin]]		[[Category: Lectin]]

OCA at 16:42, 28 September 2014

OCA — Sun, 28 Sep 2014 16:42:51 GMT

←Older revision		Revision as of 16:42, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1lgc.~~png~~\|~~left~~\|200px]]	+	==INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY==
		+	<StructureSection load='1lgc' size='340' side='right' caption='[[1lgc]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1lgc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LGC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lgc RCSB], [http://www.ebi.ac.uk/pdbsum/1lgc PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lg/1lgc_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Lectins mediate cell-cell interactions by specifically recognizing oligosaccharide chains. Legume lectins serve as mediators for the symbiotic interactions between plants and nitrogen-fixing microorganisms, an important process in the nitrogen cycle. Lectins from the Viciae tribe have a high affinity for the fucosylated biantennary N-acetyllactosamine-type glycans which are to be found in the majority of N-glycosylproteins. While the structures of several lectins complexed with incomplete oligosaccharides have been solved, no previous structure has included the complete glycoprotein. RESULTS: We have determined the crystal structures of Lathyrus ochrus isolectin II complexed with the N2 monoglycosylated fragment of human lactotransferrin (18 kDa) and an isolated glycopeptide (2.1 kDa) fragment of human lactotransferrin (at 3.3 A and 2.8 A resolution, respectively). Comparison between the two structures showed that the protein part of the glycoprotein has little influence on either the stabilization of the complex or the sugar conformation. In both cases the oligosaccharide adopts the same extended conformation. Besides the essential mannose moiety of the monosaccharide-binding site, the fucose-1' of the core has a large surface of interaction with the lectin. This oligosaccharide conformation differs substantially from that seen in the previously determined isolectin I-octasaccharide complex. Comparison of our structure with that of concanavalin A (ConA) suggests that the ConA binding site cannot accommodate this fucose. CONCLUSIONS: Our results explain the observation that Viciae lectins have a higher affinity for fucosylated oligosaccharides than for unfucosylated ones, whereas the affinity of ConA for these types of oligosaccharides is similar. This explanation is testable by mutagenesis experiments. Our structure shows a large complementary surface area between the oligosaccharide and the lectin, in contrast with the recently determined structure of a complex between the carbohydrate recognition domain of a C-type mammalian lectin and an oligomannoside, where only the non-reducing terminal mannose residue interacts with the lectin.

-	~~{{STRUCTURE_1lgc\| PDB=1lgc \| SCENE= }}~~	+	Structures of a legume lectin complexed with the human lactotransferrin N2 fragment, and with an isolated biantennary glycopeptide: role of the fucose moiety.,Bourne Y, Mazurier J, Legrand D, Rouge P, Montreuil J, Spik G, Cambillau C Structure. 1994 Mar 15;2(3):209-19. PMID:8069634<ref>PMID:8069634</ref>

-	~~===INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8069634}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1lgc]] is~~ a ~~9 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lathyrus_ochrus Lathyrus ochrus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:008069634<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Lathyrus ochrus]]		[[Category: Lathyrus ochrus]]
	[[Category: Bourne, Y.]]		[[Category: Bourne, Y.]]
	[[Category: Cambillau, C.]]		[[Category: Cambillau, C.]]
	[[Category: Lectin]]		[[Category: Lectin]]

OCA: Protected "1lgc" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 19:49:24 GMT

Protected "1lgc" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:49, 5 December 2012

OCA at 19:49, 5 December 2012

OCA — Wed, 05 Dec 2012 19:49:21 GMT

←Older revision		Revision as of 19:49, 5 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1lgc.png\|left\|200px]]		[[Image:1lgc.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1lgc", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1lgc\| PDB=1lgc \| SCENE= }}		{{STRUCTURE_1lgc\| PDB=1lgc \| SCENE= }}

	===INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY===		===INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_8069634}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 8069634 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_8069634}}		{{ABSTRACT_PUBMED_8069634}}

	==About this Structure==		==About this Structure==
-	~~1LGC~~ is a 9 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA].	+	[[1lgc]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGC OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~8069634~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008069634</ref><references group="xtra"/>
	[[Category: Lathyrus ochrus]]		[[Category: Lathyrus ochrus]]
	[[Category: Bourne, Y.]]		[[Category: Bourne, Y.]]
	[[Category: Cambillau, C.]]		[[Category: Cambillau, C.]]
	[[Category: Lectin]]		[[Category: Lectin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Sep 21 19:44:31 2009''