1ls1 - Revision history

OCA at 07:35, 14 February 2024

OCA — Wed, 14 Feb 2024 07:35:28 GMT

←Older revision		Revision as of 07:35, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1ls1' size='340' side='right'caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1ls1' size='340' side='right'caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_25104 Atcc 25104~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1ffh\|1ffh]]~~, ~~[[3ng1\|3ng1]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [https://pdbe.org/1ls1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ls1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ls1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [https://pdbe.org/1ls1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ls1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ls1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SRP54_THEAQ SRP54_THEAQ]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).[HAMAP-Rule:MF_00306]	+	[https://www.uniprot.org/uniprot/SRP54_THEAQ SRP54_THEAQ] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).[HAMAP-Rule:MF_00306]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ls1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ls1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The NG domain of the prokaryotic signal recognition protein Ffh is a two-domain GTPase that comprises part of the prokaryotic signal recognition particle (SRP) that functions in co-translational targeting of proteins to the membrane. The interface between the N and G domains includes two highly conserved sequence motifs and is adjacent in sequence and structure to one of the conserved GTPase signature motifs. Previous structural studies have shown that the relative orientation of the two domains is dynamic. The N domain of Ffh has been proposed to function in regulating the nucleotide-binding interactions of the G domain. However, biochemical studies suggest a more complex role for the domain in integrating communication between signal sequence recognition and interaction with receptor. Here, we report the structure of the apo NG GTPase of Ffh from Thermus aquaticus refined at 1.10 A resolution. Although the G domain is very well ordered in this structure, the N domain is less well ordered, reflecting the dynamic relationship between the two domains previously inferred. We demonstrate that the anisotropic displacement parameters directly visualize the underlying mobility between the two domains, and present a detailed structural analysis of the packing of the residues, including the critical alpha4 helix, that comprise the interface. Our data allows us to propose a structural explanation for the functional significance of sequence elements conserved at the N/G interface.
-
-	Structural basis for mobility in the 1.1 A crystal structure of the NG domain of Thermus aquaticus Ffh.,Ramirez UD, Minasov G, Focia PJ, Stroud RM, Walter P, Kuhn P, Freymann DM J Mol Biol. 2002 Jul 19;320(4):783-99. PMID:12095255<ref>PMID:12095255</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ls1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Signal recognition particle ~~protein~~\|Signal recognition particle ~~protein~~]]	+	*[[Signal recognition particle 3D structures\|Signal recognition particle 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 25104]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Freymann, D M~~]]	+	[[Category: Thermus aquaticus]]
-	[[Category: ~~Minasov, G~~]]	+	[[Category: Freymann DM]]
-	[[Category: ~~Ramirez, U D~~]]	+	[[Category: Minasov G]]
-	[[Category: ~~Ffh]]~~	+	[[Category: Ramirez UD]]
-	~~[[Category: Gtpase]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Srp]]~~	+
-	~~[[Category: Srp54]]~~	+
-	~~[[Category: Ultrahigh resolution~~]]	+

OCA at 05:55, 28 April 2021

OCA — Wed, 28 Apr 2021 05:55:54 GMT

←Older revision		Revision as of 05:55, 28 April 2021
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	<StructureSection load='1ls1' size='340' side='right'caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1ls1' size='340' side='right'caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_25104 Atcc 25104]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_25104 Atcc 25104]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ffh\|1ffh]], [[3ng1\|3ng1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ffh\|1ffh]], [[3ng1\|3ng1]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [~~http~~://pdbe.org/1ls1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ls1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ls1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [https://pdbe.org/1ls1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ls1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ls1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SRP54_THEAQ SRP54_THEAQ]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).[HAMAP-Rule:MF_00306]	+	[[https://www.uniprot.org/uniprot/SRP54_THEAQ SRP54_THEAQ]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).[HAMAP-Rule:MF_00306]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:28, 24 July 2019

OCA — Wed, 24 Jul 2019 09:28:36 GMT

←Older revision		Revision as of 09:28, 24 July 2019
Line 1:		Line 1:

	==T. aquaticus Ffh NG Domain at 1.1A Resolution==		==T. aquaticus Ffh NG Domain at 1.1A Resolution==
-	<StructureSection load='1ls1' size='340' side='right' caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='1ls1' size='340' side='right'caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25104 Atcc 25104]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25104 Atcc 25104]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ls/1ls1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ls/1ls1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1ls1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ls1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Signal recognition particle protein\|Signal recognition particle protein]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 25104]]		[[Category: Atcc 25104]]
		+	[[Category: Large Structures]]
	[[Category: Freymann, D M]]		[[Category: Freymann, D M]]
	[[Category: Minasov, G]]		[[Category: Minasov, G]]

OCA at 10:42, 18 October 2017

OCA — Wed, 18 Oct 2017 10:42:22 GMT

←Older revision		Revision as of 10:42, 18 October 2017
Line 1:		Line 1:
		+
	==T. aquaticus Ffh NG Domain at 1.1A Resolution==		==T. aquaticus Ffh NG Domain at 1.1A Resolution==
	<StructureSection load='1ls1' size='340' side='right' caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1ls1' size='340' side='right' caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ffh\|1ffh]], [[3ng1\|3ng1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ffh\|1ffh]], [[3ng1\|3ng1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [http://pdbe.org/1ls1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ls1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [http://pdbe.org/1ls1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ls1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ls1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ls1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1ls1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ls1" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Signal recognition particle protein\|Signal recognition particle protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:08, 12 September 2015

OCA — Sat, 12 Sep 2015 00:08:58 GMT

←Older revision		Revision as of 00:08, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1ls1' size='340' side='right' caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1ls1' size='340' side='right' caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_aquaticus Thermus aquaticus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25104 Atcc 25104]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ffh\|1ffh]], [[3ng1\|3ng1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ffh\|1ffh]], [[3ng1\|3ng1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ls1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [http://pdbe.org/1ls1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ls1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ls1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermus aquaticus~~]]	+	[[Category: Atcc 25104]]
	[[Category: Freymann, D M]]		[[Category: Freymann, D M]]
	[[Category: Minasov, G]]		[[Category: Minasov, G]]

OCA at 21:13, 25 December 2014

OCA — Thu, 25 Dec 2014 21:13:15 GMT

←Older revision		Revision as of 21:13, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ffh\|1ffh]], [[3ng1\|3ng1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ffh\|1ffh]], [[3ng1\|3ng1]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ls1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ls1 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SRP54_THEAQ SRP54_THEAQ]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).[HAMAP-Rule:MF_00306]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Thermus aquaticus]]		[[Category: Thermus aquaticus]]
-	[[Category: Freymann, D M.]]	+	[[Category: Freymann, D M]]
-	[[Category: Minasov, G.]]	+	[[Category: Minasov, G]]
-	[[Category: Ramirez, U D.]]	+	[[Category: Ramirez, U D]]
	[[Category: Ffh]]		[[Category: Ffh]]
	[[Category: Gtpase]]		[[Category: Gtpase]]

OCA at 15:43, 28 September 2014

OCA — Sun, 28 Sep 2014 15:43:37 GMT

←Older revision		Revision as of 15:43, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ls1.~~png~~\|~~left~~\|200px]]	+	==T. aquaticus Ffh NG Domain at 1.1A Resolution==
		+	<StructureSection load='1ls1' size='340' side='right' caption='[[1ls1]], [[Resolution\|resolution]] 1.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ls1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LS1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ffh\|1ffh]], [[3ng1\|3ng1]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ls1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ls1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ls1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ls/1ls1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The NG domain of the prokaryotic signal recognition protein Ffh is a two-domain GTPase that comprises part of the prokaryotic signal recognition particle (SRP) that functions in co-translational targeting of proteins to the membrane. The interface between the N and G domains includes two highly conserved sequence motifs and is adjacent in sequence and structure to one of the conserved GTPase signature motifs. Previous structural studies have shown that the relative orientation of the two domains is dynamic. The N domain of Ffh has been proposed to function in regulating the nucleotide-binding interactions of the G domain. However, biochemical studies suggest a more complex role for the domain in integrating communication between signal sequence recognition and interaction with receptor. Here, we report the structure of the apo NG GTPase of Ffh from Thermus aquaticus refined at 1.10 A resolution. Although the G domain is very well ordered in this structure, the N domain is less well ordered, reflecting the dynamic relationship between the two domains previously inferred. We demonstrate that the anisotropic displacement parameters directly visualize the underlying mobility between the two domains, and present a detailed structural analysis of the packing of the residues, including the critical alpha4 helix, that comprise the interface. Our data allows us to propose a structural explanation for the functional significance of sequence elements conserved at the N/G interface.

-	~~{{STRUCTURE_1ls1\| PDB=1ls1 \| SCENE= }}~~	+	Structural basis for mobility in the 1.1 A crystal structure of the NG domain of Thermus aquaticus Ffh.,Ramirez UD, Minasov G, Focia PJ, Stroud RM, Walter P, Kuhn P, Freymann DM J Mol Biol. 2002 Jul 19;320(4):783-99. PMID:12095255<ref>PMID:12095255</ref>

-	~~===T. aquaticus Ffh NG Domain at 1.1A Resolution===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12095255}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1ls1]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermus_aquaticus Thermus aquaticus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA].~~	+

	==See Also==		==See Also==
	*[[Signal recognition particle protein\|Signal recognition particle protein]]		*[[Signal recognition particle protein\|Signal recognition particle protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012095255<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Thermus aquaticus]]		[[Category: Thermus aquaticus]]
	[[Category: Freymann, D M.]]		[[Category: Freymann, D M.]]

OCA at 21:43, 20 October 2012

OCA — Sat, 20 Oct 2012 21:43:24 GMT

←Older revision		Revision as of 21:43, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1ls1]] is a 1 chain structure ~~of [[Signal recognition particle protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA].	+	[[1ls1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA].

	==See Also==		==See Also==

OCA: Protected "1ls1" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 14:05:17 GMT

Protected "1ls1" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:05, 27 July 2012

OCA at 14:05, 27 July 2012

OCA — Fri, 27 Jul 2012 14:05:14 GMT

←Older revision		Revision as of 14:05, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1ls1.png\|left\|200px]]		[[Image:1ls1.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ls1", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ls1\| PDB=1ls1 \| SCENE= }}		{{STRUCTURE_1ls1\| PDB=1ls1 \| SCENE= }}

	===T. aquaticus Ffh NG Domain at 1.1A Resolution===		===T. aquaticus Ffh NG Domain at 1.1A Resolution===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12095255}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12095255 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12095255}}		{{ABSTRACT_PUBMED_12095255}}

	==About this Structure==		==About this Structure==
-	~~1LS1~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA].	+	[[1ls1]] is a 1 chain structure of [[Signal recognition particle protein]] with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS1 OCA].
		+
		+	==See Also==
		+	*[[Signal recognition particle protein\|Signal recognition particle protein]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12095255~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012095255</ref><references group="xtra"/>
	[[Category: Thermus aquaticus]]		[[Category: Thermus aquaticus]]
	[[Category: Freymann, D M.]]		[[Category: Freymann, D M.]]
Line 30:		Line 21:
	[[Category: Ffh]]		[[Category: Ffh]]
	[[Category: Gtpase]]		[[Category: Gtpase]]
		+	[[Category: Protein transport]]
	[[Category: Srp]]		[[Category: Srp]]
	[[Category: Srp54]]		[[Category: Srp54]]
	[[Category: Ultrahigh resolution]]		[[Category: Ultrahigh resolution]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:46:56 2009''