1lvk - Revision history

OCA at 07:36, 14 February 2024

OCA — Wed, 14 Feb 2024 07:36:46 GMT

←Older revision		Revision as of 07:36, 14 February 2024
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	<StructureSection load='1lvk' size='340' side='right'caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1lvk' size='340' side='right'caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_11735 Atcc 11735~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LVK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LVK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [https://pdbe.org/1lvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [https://www.ebi.ac.uk/pdbsum/1lvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [https://pdbe.org/1lvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [https://www.ebi.ac.uk/pdbsum/1lvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.	+	[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lvk ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lvk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Mant (2'(3')-O-(N-methylanthraniloyl)) labeled nucleotides have proven to be useful tools in the study of the kinetic mechanism of the myosin ATPase by fluorescence spectroscopy. The sensitivity of the mant fluorophore to its local environment also makes it suitable to investigate the exposure of bound nucleotides to solvent from collisional quenching measurements. Here we present the crystal structure of mant-ADP and beryllium fluoride complexed with Dictyostelium discoideum myosin motor domain (S1dC) at 1.9 A resolution. We complement the structural approach with an investigation of the accessibility of the mant moiety to solvent using acrylamide quenching of fluorescence emission. In contrast to rabbit skeletal myosin subfragment 1, where the mant group is protected from acrylamide (Ksv=0.2 M-1), the fluorophore is relatively exposed when bound to Dictyostelium myosin motor domain (Ksv= 1.4 M-1). Differences between the Dictyostelium structure and that of vertebrate skeletal subfragment 1, in the region of the nucleotide binding pocket, are proposed as an explanation for the differences observed in the solvent accessibility of complexed mant-nucleotides. We conclude that protection of the mant group from acrylamide quenching does not report on overall closure of the nucleotide binding pocket but reflects more local structural changes.
-
-	X-ray crystal structure and solution fluorescence characterization of Mg.2'(3')-O-(N-methylanthraniloyl) nucleotides bound to the Dictyostelium discoideum myosin motor domain.,Bauer CB, Kuhlman PA, Bagshaw CR, Rayment I J Mol Biol. 1997 Dec 5;274(3):394-407. PMID:9405148<ref>PMID:9405148</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1lvk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Myosin 3D Structures\|Myosin 3D Structures]]		*[[Myosin 3D Structures\|Myosin 3D Structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 11735~~]]	+	[[Category: Dictyostelium discoideum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bagshaw~~, C R~~]]	+	[[Category: Bagshaw CR]]
-	[[Category: Bauer~~, C B~~]]	+	[[Category: Bauer CB]]
-	[[Category: Kuhlman~~, P A~~]]	+	[[Category: Kuhlman PA]]
-	[[Category: Rayment, I]]	+	[[Category: Rayment I]]
-	~~[[Category: Actin-binding]]~~	+
-	~~[[Category: Atpase]]~~	+
-	~~[[Category: Coiled coil]]~~	+
-	~~[[Category: Contractile protein]]~~	+
-	~~[[Category: Dictyostelium]]~~	+
-	~~[[Category: Mant]]~~	+
-	~~[[Category: Motor]]~~	+
-	~~[[Category: Myosin~~]]	+

OCA at 06:49, 18 August 2021

OCA — Wed, 18 Aug 2021 06:49:38 GMT

←Older revision		Revision as of 06:49, 18 August 2021
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	<StructureSection load='1lvk' size='340' side='right'caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1lvk' size='340' side='right'caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1LVK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LVK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [~~http~~://pdbe.org/1lvk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1lvk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [https://pdbe.org/1lvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [https://www.ebi.ac.uk/pdbsum/1lvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.	+	[[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:18, 13 November 2019

OCA — Wed, 13 Nov 2019 12:18:28 GMT

←Older revision		Revision as of 12:18, 13 November 2019
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	==X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN==		==X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN==
-	<StructureSection load='1lvk' size='340' side='right' caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1lvk' size='340' side='right'caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LVK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LVK FirstGlance]. <br>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1lvk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1lvk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Myosin 3D Structures\|Myosin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 11735]]		[[Category: Atcc 11735]]
		+	[[Category: Large Structures]]
	[[Category: Bagshaw, C R]]		[[Category: Bagshaw, C R]]
	[[Category: Bauer, C B]]		[[Category: Bauer, C B]]

OCA at 07:54, 31 January 2018

OCA — Wed, 31 Jan 2018 07:54:24 GMT

←Older revision		Revision as of 07:54, 31 January 2018
Line 1:		Line 1:
		+
	==X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN==		==X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN==
	<StructureSection load='1lvk' size='340' side='right' caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1lvk' size='340' side='right' caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LVK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LVK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [http://pdbe.org/1lvk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [http://www.ebi.ac.uk/pdbsum/1lvk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [http://pdbe.org/1lvk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [http://www.ebi.ac.uk/pdbsum/1lvk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/1lvk_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/1lvk_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1lvk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1lvk" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Myosin\|Myosin]]
	== References ==		== References ==
	<references/>		<references/>
Line 41:		Line 39:
	[[Category: Actin-binding]]		[[Category: Actin-binding]]
	[[Category: Atpase]]		[[Category: Atpase]]
		+	[[Category: Coiled coil]]
	[[Category: Contractile protein]]		[[Category: Contractile protein]]
	[[Category: Dictyostelium]]		[[Category: Dictyostelium]]

OCA at 02:35, 7 February 2016

OCA — Sun, 07 Feb 2016 02:35:50 GMT

←Older revision		Revision as of 02:35, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lvk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:51, 9 September 2015

OCA — Wed, 09 Sep 2015 20:51:24 GMT

←Older revision		Revision as of 20:51, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1lvk' size='340' side='right' caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1lvk' size='340' side='right' caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Dictyostelium_discoideum Dictyostelium discoideum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LVK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LVK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [http://www.ebi.ac.uk/pdbsum/1lvk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [http://pdbe.org/1lvk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [http://www.ebi.ac.uk/pdbsum/1lvk PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1lvk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dictyostelium discoideum~~]]	+	[[Category: Atcc 11735]]
	[[Category: Bagshaw, C R]]		[[Category: Bagshaw, C R]]
	[[Category: Bauer, C B]]		[[Category: Bauer, C B]]

OCA at 07:09, 24 December 2014

OCA — Wed, 24 Dec 2014 07:09:26 GMT

←Older revision		Revision as of 07:09, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LVK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LVK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [http://www.ebi.ac.uk/pdbsum/1lvk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [http://www.ebi.ac.uk/pdbsum/1lvk PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
-	[[Category: Bagshaw, C R.]]	+	[[Category: Bagshaw, C R]]
-	[[Category: Bauer, C B.]]	+	[[Category: Bauer, C B]]
-	[[Category: Kuhlman, P A.]]	+	[[Category: Kuhlman, P A]]
-	[[Category: Rayment, I.]]	+	[[Category: Rayment, I]]
	[[Category: Actin-binding]]		[[Category: Actin-binding]]
	[[Category: Atpase]]		[[Category: Atpase]]

OCA at 13:17, 28 September 2014

OCA — Sun, 28 Sep 2014 13:17:05 GMT

←Older revision		Revision as of 13:17, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1lvk.~~png\|left~~\|200px]]	+	==X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN==
		+	<StructureSection load='1lvk' size='340' side='right' caption='[[1lvk]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1lvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LVK FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MNT:2(3)-O-N-METHYLANTHRANILOYL-ADENOSINE-5-DIPHOSPHATE'>MNT</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lvk RCSB], [http://www.ebi.ac.uk/pdbsum/1lvk PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/1lvk_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mant (2'(3')-O-(N-methylanthraniloyl)) labeled nucleotides have proven to be useful tools in the study of the kinetic mechanism of the myosin ATPase by fluorescence spectroscopy. The sensitivity of the mant fluorophore to its local environment also makes it suitable to investigate the exposure of bound nucleotides to solvent from collisional quenching measurements. Here we present the crystal structure of mant-ADP and beryllium fluoride complexed with Dictyostelium discoideum myosin motor domain (S1dC) at 1.9 A resolution. We complement the structural approach with an investigation of the accessibility of the mant moiety to solvent using acrylamide quenching of fluorescence emission. In contrast to rabbit skeletal myosin subfragment 1, where the mant group is protected from acrylamide (Ksv=0.2 M-1), the fluorophore is relatively exposed when bound to Dictyostelium myosin motor domain (Ksv= 1.4 M-1). Differences between the Dictyostelium structure and that of vertebrate skeletal subfragment 1, in the region of the nucleotide binding pocket, are proposed as an explanation for the differences observed in the solvent accessibility of complexed mant-nucleotides. We conclude that protection of the mant group from acrylamide quenching does not report on overall closure of the nucleotide binding pocket but reflects more local structural changes.

-	~~{{STRUCTURE_1lvk\| PDB=1lvk \| SCENE= }}~~	+	X-ray crystal structure and solution fluorescence characterization of Mg.2'(3')-O-(N-methylanthraniloyl) nucleotides bound to the Dictyostelium discoideum myosin motor domain.,Bauer CB, Kuhlman PA, Bagshaw CR, Rayment I J Mol Biol. 1997 Dec 5;274(3):394-407. PMID:9405148<ref>PMID:9405148</ref>

-	~~===X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9405148}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1lvk]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA].~~	+

	==See Also==		==See Also==
	*[[Myosin\|Myosin]]		*[[Myosin\|Myosin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009405148<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
	[[Category: Bagshaw, C R.]]		[[Category: Bagshaw, C R.]]

OCA at 05:21, 20 October 2012

OCA — Sat, 20 Oct 2012 05:21:31 GMT

←Older revision		Revision as of 05:21, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1lvk]] is a 1 chain structure ~~of [[Myosin]]~~ with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA].	+	[[1lvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVK OCA].

	==See Also==		==See Also==

OCA at 09:47, 25 July 2012

OCA — Wed, 25 Jul 2012 09:47:46 GMT

←Older revision		Revision as of 09:47, 25 July 2012
Line 1:		Line 1:
	[[Image:1lvk.png\|left\|200px]]		[[Image:1lvk.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1lvk\| PDB=1lvk \| SCENE= }}		{{STRUCTURE_1lvk\| PDB=1lvk \| SCENE= }}

	===X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN===		===X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN===

-
-	<!--
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	{{ABSTRACT_PUBMED_9405148}}		{{ABSTRACT_PUBMED_9405148}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Myosin]]	+	*[[Myosin\|Myosin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9405148~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009405148</ref><references group="xtra"/>
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
	[[Category: Bagshaw, C R.]]		[[Category: Bagshaw, C R.]]
Line 33:		Line 22:
	[[Category: Actin-binding]]		[[Category: Actin-binding]]
	[[Category: Atpase]]		[[Category: Atpase]]
-	[[Category: Coiled coil]]
	[[Category: Contractile protein]]		[[Category: Contractile protein]]
	[[Category: Dictyostelium]]		[[Category: Dictyostelium]]