1lw1 - Revision history

OCA at 07:17, 25 October 2023

OCA — Wed, 25 Oct 2023 07:17:46 GMT

←Older revision		Revision as of 07:17, 25 October 2023
Line 3:		Line 3:
	<StructureSection load='1lw1' size='340' side='right'caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1lw1' size='340' side='right'caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>~~[[~~1gu9~~\|~~1gu9~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [https://pdbe.org/1lw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [https://www.ebi.ac.uk/pdbsum/1lw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lw1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [https://pdbe.org/1lw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [https://www.ebi.ac.uk/pdbsum/1lw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lw1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AHPD_MYCTU AHPD_MYCTU]] Antioxidant protein with alkyl hydroperoxidase activity. Required for the reduction of the AhpC active site cysteine residues and for the regeneration of the AhpC enzyme activity.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref> Together with AhpC, DlaT and Lpd, constitutes an NADH-dependent peroxidase active against hydrogen and alkyl peroxides as well as serving as a peroxynitrite reductase, thus protecting the bacterium against reactive nitrogen intermediates and oxidative stress generated by the host immune system.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref>	+	[https://www.uniprot.org/uniprot/AHPD_MYCTU AHPD_MYCTU] Antioxidant protein with alkyl hydroperoxidase activity. Required for the reduction of the AhpC active site cysteine residues and for the regeneration of the AhpC enzyme activity.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref> Together with AhpC, DlaT and Lpd, constitutes an NADH-dependent peroxidase active against hydrogen and alkyl peroxides as well as serving as a peroxynitrite reductase, thus protecting the bacterium against reactive nitrogen intermediates and oxidative stress generated by the host immune system.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Djordjevic, S]]	+	[[Category: Djordjevic S]]
-	~~[[Category: Montellano, P R.Ortiz de~~]]	+	[[Category: Nunn CM]]
-	[[Category: Nunn~~, C M~~]]	+	[[Category: Ortiz de Montellano PR]]
-	[[Category: ~~Alkylhydroperoxidase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Tuberculosis~~]]	+

OCA at 06:49, 18 August 2021

OCA — Wed, 18 Aug 2021 06:49:46 GMT

←Older revision		Revision as of 06:49, 18 August 2021
Line 3:		Line 3:
	<StructureSection load='1lw1' size='340' side='right'caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1lw1' size='340' side='right'caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gu9\|1gu9]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gu9\|1gu9]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [~~http~~://pdbe.org/1lw1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1lw1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1lw1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [https://pdbe.org/1lw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [https://www.ebi.ac.uk/pdbsum/1lw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lw1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AHPD_MYCTU AHPD_MYCTU]] Antioxidant protein with alkyl hydroperoxidase activity. Required for the reduction of the AhpC active site cysteine residues and for the regeneration of the AhpC enzyme activity.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref> Together with AhpC, DlaT and Lpd, constitutes an NADH-dependent peroxidase active against hydrogen and alkyl peroxides as well as serving as a peroxynitrite reductase, thus protecting the bacterium against reactive nitrogen intermediates and oxidative stress generated by the host immune system.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref>	+	[[https://www.uniprot.org/uniprot/AHPD_MYCTU AHPD_MYCTU]] Antioxidant protein with alkyl hydroperoxidase activity. Required for the reduction of the AhpC active site cysteine residues and for the regeneration of the AhpC enzyme activity.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref> Together with AhpC, DlaT and Lpd, constitutes an NADH-dependent peroxidase active against hydrogen and alkyl peroxides as well as serving as a peroxynitrite reductase, thus protecting the bacterium against reactive nitrogen intermediates and oxidative stress generated by the host immune system.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:14, 13 November 2019

OCA — Wed, 13 Nov 2019 12:14:56 GMT

←Older revision		Revision as of 12:14, 13 November 2019
Line 1:		Line 1:

	==Crystal Structure Of Mycobacterium Tuberculosis Alkylperoxidase Ahpd H137F mutant==		==Crystal Structure Of Mycobacterium Tuberculosis Alkylperoxidase Ahpd H137F mutant==
-	<StructureSection load='1lw1' size='340' side='right' caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='1lw1' size='340' side='right'caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Myctu]]		[[Category: Myctu]]
	[[Category: Djordjevic, S]]		[[Category: Djordjevic, S]]

OCA at 07:50, 31 January 2018

OCA — Wed, 31 Jan 2018 07:50:49 GMT

←Older revision		Revision as of 07:50, 31 January 2018
Line 1:		Line 1:
		+
	==Crystal Structure Of Mycobacterium Tuberculosis Alkylperoxidase Ahpd H137F mutant==		==Crystal Structure Of Mycobacterium Tuberculosis Alkylperoxidase Ahpd H137F mutant==
	<StructureSection load='1lw1' size='340' side='right' caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1lw1' size='340' side='right' caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gu9\|1gu9]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gu9\|1gu9]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [http://pdbe.org/1lw1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [http://www.ebi.ac.uk/pdbsum/1lw1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [http://pdbe.org/1lw1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [http://www.ebi.ac.uk/pdbsum/1lw1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lw1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lw/1lw1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lw/1lw1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 04:18, 9 February 2016

OCA — Tue, 09 Feb 2016 04:18:24 GMT

←Older revision		Revision as of 04:18, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lw1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 18:33, 10 September 2015

OCA — Thu, 10 Sep 2015 18:33:35 GMT

←Older revision		Revision as of 18:33, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1lw1' size='340' side='right' caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1lw1' size='340' side='right' caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium tuberculosis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gu9\|1gu9]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gu9\|1gu9]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [http://www.ebi.ac.uk/pdbsum/1lw1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [http://pdbe.org/1lw1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [http://www.ebi.ac.uk/pdbsum/1lw1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1lw1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mycobacterium tuberculosis~~]]	+	[[Category: Myctu]]
	[[Category: Djordjevic, S]]		[[Category: Djordjevic, S]]
	[[Category: Montellano, P R.Ortiz de]]		[[Category: Montellano, P R.Ortiz de]]

OCA at 04:22, 25 December 2014

OCA — Thu, 25 Dec 2014 04:22:46 GMT

←Older revision		Revision as of 04:22, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gu9\|1gu9]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gu9\|1gu9]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [http://www.ebi.ac.uk/pdbsum/1lw1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [http://www.ebi.ac.uk/pdbsum/1lw1 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AHPD_MYCTU AHPD_MYCTU]] Antioxidant protein with alkyl hydroperoxidase activity. Required for the reduction of the AhpC active site cysteine residues and for the regeneration of the AhpC enzyme activity.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref> Together with AhpC, DlaT and Lpd, constitutes an NADH-dependent peroxidase active against hydrogen and alkyl peroxides as well as serving as a peroxynitrite reductase, thus protecting the bacterium against reactive nitrogen intermediates and oxidative stress generated by the host immune system.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
-	[[Category: Djordjevic, S.]]	+	[[Category: Djordjevic, S]]
-	[[Category: Montellano, P R.Ortiz de.]]	+	[[Category: Montellano, P R.Ortiz de]]
-	[[Category: Nunn, C M.]]	+	[[Category: Nunn, C M]]
	[[Category: Alkylhydroperoxidase]]		[[Category: Alkylhydroperoxidase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Tuberculosis]]		[[Category: Tuberculosis]]

OCA at 14:59, 28 September 2014

OCA — Sun, 28 Sep 2014 14:59:01 GMT

←Older revision		Revision as of 14:59, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1lw1.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure Of Mycobacterium Tuberculosis Alkylperoxidase Ahpd H137F mutant==
		+	<StructureSection load='1lw1' size='340' side='right' caption='[[1lw1]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1lw1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LW1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gu9\|1gu9]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lw1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lw1 RCSB], [http://www.ebi.ac.uk/pdbsum/1lw1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lw/1lw1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	AhpD, a protein with two cysteine residues, is required for physiological reduction of the Mycobacterium tuberculosis alkylhydroperoxidase AhpC. AhpD also has an alkylhydroperoxidase activity of its own. The AhpC/AhpD system provides critical antioxidant protection, particularly in the absence of the catalase-peroxidase KatG, which is suppressed in most isoniazid-resistant strains. Based on the crystal structure, we proposed recently a catalytic mechanism for AhpD involving a proton relay in which the Glu118 carboxylate group, via His137 and a water molecule, deprotonates the catalytic residue Cys133 (Nunn, C. M., Djordjevic, S., Hillas, P. J., Nishida, C., and Ortiz de Montellano, P. R. (2002) J. Biol. Chem. 277, 20033-20040). A possible role for His132 in subsequent formation of the Cys133-Cys130 disulfide bond was also noted. To test this proposed mechanism, we have expressed the H137F, H137Q, H132F, H132Q, E118F, E118Q, C133S, and C130S mutants of AhpD, determined the crystal structures of the H137F and H132Q mutants, estimated the pKa values of the cysteine residues, and defined the kinetic properties of the mutant proteins. The collective results strongly support the proposed catalytic mechanism for AhpD.

-	~~{{STRUCTURE_1lw1\| PDB=1lw1 \| SCENE= }}~~	+	The mechanism of Mycobacterium tuberculosis alkylhydroperoxidase AhpD as defined by mutagenesis, crystallography, and kinetics.,Koshkin A, Nunn CM, Djordjevic S, Ortiz de Montellano PR J Biol Chem. 2003 Aug 8;278(32):29502-8. Epub 2003 May 21. PMID:12761216<ref>PMID:12761216</ref>

-	~~===Crystal Structure Of Mycobacterium Tuberculosis Alkylperoxidase Ahpd H137F mutant===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12761216}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1lw1]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012761216<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Djordjevic, S.]]		[[Category: Djordjevic, S.]]

OCA: Protected "1lw1" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 20:51:58 GMT

Protected "1lw1" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:51, 5 December 2012

OCA at 20:51, 5 December 2012

OCA — Wed, 05 Dec 2012 20:51:53 GMT

←Older revision		Revision as of 20:51, 5 December 2012
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-	{{Seed}}
	[[Image:1lw1.png\|left\|200px]]		[[Image:1lw1.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1lw1\| PDB=1lw1 \| SCENE= }}		{{STRUCTURE_1lw1\| PDB=1lw1 \| SCENE= }}

	===Crystal Structure Of Mycobacterium Tuberculosis Alkylperoxidase Ahpd H137F mutant===		===Crystal Structure Of Mycobacterium Tuberculosis Alkylperoxidase Ahpd H137F mutant===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12761216}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_12761216}}		{{ABSTRACT_PUBMED_12761216}}

	==About this Structure==		==About this Structure==
-	~~1LW1~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA].	+	[[1lw1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LW1 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12761216~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012761216</ref><references group="xtra"/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Djordjevic, S.]]		[[Category: Djordjevic, S.]]
Line 29:		Line 17:
	[[Category: Nunn, C M.]]		[[Category: Nunn, C M.]]
	[[Category: Alkylhydroperoxidase]]		[[Category: Alkylhydroperoxidase]]
		+	[[Category: Oxidoreductase]]
	[[Category: Tuberculosis]]		[[Category: Tuberculosis]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:24:52 2009''