1m5x - Revision history

OCA at 07:40, 14 February 2024

OCA — Wed, 14 Feb 2024 07:40:13 GMT

←Older revision		Revision as of 07:40, 14 February 2024
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	<StructureSection load='1m5x' size='340' side='right'caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='1m5x' size='340' side='right'caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Monsk Monsk~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M5X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Monomastix_sp._OKE-1 Monomastix sp. OKE-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M5X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [https://pdbe.org/1m5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [https://www.ebi.ac.uk/pdbsum/1m5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m5x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [https://pdbe.org/1m5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [https://www.ebi.ac.uk/pdbsum/1m5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m5x ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C0JWR6_MONSK C0JWR6_MONSK]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m5x ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m5x ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Homing endonucleases are highly specific catalysts of DNA strand breaks that induce the transposition of mobile intervening sequences containing the endonuclease open reading frame. These enzymes recognize long DNA targets while tolerating individual sequence polymorphisms within those sites. Sequences of the homing endonucleases themselves diversify to a great extent after founding intron invasion events, generating highly divergent enzymes that recognize similar target sequences. Here, we visualize the mechanism of flexible DNA recognition and the pattern of structural divergence displayed by two homing endonuclease isoschizomers. We determined structures of I-CreI bound to two DNA target sites that differ at eight of 22 base-pairs, and the structure of an isoschizomer, I-MsoI, bound to a nearly identical DNA target site. This study illustrates several principles governing promiscuous base-pair recognition by DNA-binding proteins, and demonstrates that the isoschizomers display strikingly different protein/DNA contacts. The structures allow us to determine the information content at individual positions in the binding site as a function of the distribution of direct and water-mediated contacts to nucleotide bases, and provide an evolutionary snapshot of endonucleases at an early stage of divergence in their target specificity.
-
-	Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI.,Chevalier B, Turmel M, Lemieux C, Monnat RJ Jr, Stoddard BL J Mol Biol. 2003 May 30;329(2):253-69. PMID:12758074<ref>PMID:12758074</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1m5x" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]		*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Monsk~~]]	+	[[Category: Monomastix sp. OKE-1]]
-	[[Category: Chevalier, B]]	+	[[Category: Chevalier B]]
-	[[Category: Lemieux, C]]	+	[[Category: Lemieux C]]
-	[[Category: Monnat~~, R J~~]]	+	[[Category: Monnat RJ]]
-	[[Category: Stoddard~~, B L~~]]	+	[[Category: Stoddard BL]]
-	[[Category: Turmel, M]]	+	[[Category: Turmel M]]
-	~~[[Category: Hydrolase-dna complex]]~~	+
-	~~[[Category: Laglidadg~~]]	+

OCA at 09:52, 15 September 2021

OCA — Wed, 15 Sep 2021 09:52:19 GMT

←Older revision		Revision as of 09:52, 15 September 2021
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	<StructureSection load='1m5x' size='340' side='right'caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='1m5x' size='340' side='right'caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Monsk Monsk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1M5X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Monsk Monsk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M5X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [~~http~~://pdbe.org/1m5x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1m5x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1m5x ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [https://pdbe.org/1m5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [https://www.ebi.ac.uk/pdbsum/1m5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m5x ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 19:30, 11 December 2019

OCA — Wed, 11 Dec 2019 19:30:44 GMT

←Older revision		Revision as of 19:30, 11 December 2019
Line 1:		Line 1:

	==Crystal structure of the homing endonuclease I-MsoI bound to its DNA substrate==		==Crystal structure of the homing endonuclease I-MsoI bound to its DNA substrate==
-	<StructureSection load='1m5x' size='340' side='right' caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='1m5x' size='340' side='right'caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Monsk Monsk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M5X FirstGlance]. <br>		<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Monsk Monsk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M5X FirstGlance]. <br>
Line 28:		Line 28:

	==See Also==		==See Also==
-	*[[Endonuclease\|Endonuclease]]	+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Monsk]]		[[Category: Monsk]]
	[[Category: Chevalier, B]]		[[Category: Chevalier, B]]

OCA at 05:28, 8 March 2018

OCA — Thu, 08 Mar 2018 05:28:02 GMT

←Older revision		Revision as of 05:28, 8 March 2018
Line 26:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1m5x" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1m5x" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Endonuclease\|Endonuclease]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:02, 31 January 2018

OCA — Wed, 31 Jan 2018 08:02:02 GMT

←Older revision		Revision as of 08:02, 31 January 2018
Line 1:		Line 1:
		+
	==Crystal structure of the homing endonuclease I-MsoI bound to its DNA substrate==		==Crystal structure of the homing endonuclease I-MsoI bound to its DNA substrate==
	<StructureSection load='1m5x' size='340' side='right' caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='1m5x' size='340' side='right' caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Monsk Monsk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M5X FirstGlance]. <br>		<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Monsk Monsk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M5X FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [http://pdbe.org/1m5x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [http://www.ebi.ac.uk/pdbsum/1m5x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [http://pdbe.org/1m5x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [http://www.ebi.ac.uk/pdbsum/1m5x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1m5x ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m5/1m5x_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m5/1m5x_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 25:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1m5x" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1m5x" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Endonuclease\|Endonuclease]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:20, 8 February 2016

OCA — Mon, 08 Feb 2016 06:20:23 GMT

←Older revision		Revision as of 06:20, 8 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m5x ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:44, 10 September 2015

OCA — Thu, 10 Sep 2015 08:44:36 GMT

←Older revision		Revision as of 08:44, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1m5x' size='340' side='right' caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='1m5x' size='340' side='right' caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Monomastix_sp._oke-1 Monomastix sp. oke-1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M5X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Monsk Monsk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M5X FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [http://www.ebi.ac.uk/pdbsum/1m5x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [http://pdbe.org/1m5x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [http://www.ebi.ac.uk/pdbsum/1m5x PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1m5x" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Monomastix sp. oke-1~~]]	+	[[Category: Monsk]]
	[[Category: Chevalier, B]]		[[Category: Chevalier, B]]
	[[Category: Lemieux, C]]		[[Category: Lemieux, C]]

OCA at 15:54, 5 January 2015

OCA — Mon, 05 Jan 2015 15:54:30 GMT

←Older revision		Revision as of 15:54, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Monomastix_sp._oke-1 Monomastix sp. oke-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M5X FirstGlance]. <br>		<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Monomastix_sp._oke-1 Monomastix sp. oke-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M5X FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [http://www.ebi.ac.uk/pdbsum/1m5x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [http://www.ebi.ac.uk/pdbsum/1m5x PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Monomastix sp. oke-1]]		[[Category: Monomastix sp. oke-1]]
-	[[Category: Chevalier, B.]]	+	[[Category: Chevalier, B]]
-	[[Category: Lemieux, C.]]	+	[[Category: Lemieux, C]]
-	[[Category: Monnat, R J.]]	+	[[Category: Monnat, R J]]
-	[[Category: Stoddard, B L.]]	+	[[Category: Stoddard, B L]]
-	[[Category: Turmel, M.]]	+	[[Category: Turmel, M]]
	[[Category: Hydrolase-dna complex]]		[[Category: Hydrolase-dna complex]]
	[[Category: Laglidadg]]		[[Category: Laglidadg]]

OCA at 17:06, 28 September 2014

OCA — Sun, 28 Sep 2014 17:06:02 GMT

←Older revision		Revision as of 17:06, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1m5x.~~png\|left~~\|200px]]	+	==Crystal structure of the homing endonuclease I-MsoI bound to its DNA substrate==
		+	<StructureSection load='1m5x' size='340' side='right' caption='[[1m5x]], [[Resolution\|resolution]] 2.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1m5x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Monomastix_sp._oke-1 Monomastix sp. oke-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M5X FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m5x RCSB], [http://www.ebi.ac.uk/pdbsum/1m5x PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m5/1m5x_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Homing endonucleases are highly specific catalysts of DNA strand breaks that induce the transposition of mobile intervening sequences containing the endonuclease open reading frame. These enzymes recognize long DNA targets while tolerating individual sequence polymorphisms within those sites. Sequences of the homing endonucleases themselves diversify to a great extent after founding intron invasion events, generating highly divergent enzymes that recognize similar target sequences. Here, we visualize the mechanism of flexible DNA recognition and the pattern of structural divergence displayed by two homing endonuclease isoschizomers. We determined structures of I-CreI bound to two DNA target sites that differ at eight of 22 base-pairs, and the structure of an isoschizomer, I-MsoI, bound to a nearly identical DNA target site. This study illustrates several principles governing promiscuous base-pair recognition by DNA-binding proteins, and demonstrates that the isoschizomers display strikingly different protein/DNA contacts. The structures allow us to determine the information content at individual positions in the binding site as a function of the distribution of direct and water-mediated contacts to nucleotide bases, and provide an evolutionary snapshot of endonucleases at an early stage of divergence in their target specificity.

-	~~{{STRUCTURE_1m5x\| PDB=1m5x \| SCENE= }}~~	+	Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI.,Chevalier B, Turmel M, Lemieux C, Monnat RJ Jr, Stoddard BL J Mol Biol. 2003 May 30;329(2):253-69. PMID:12758074<ref>PMID:12758074</ref>

-	~~===Crystal structure~~ of the ~~homing endonuclease I-MsoI bound to its DNA substrate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12758074}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1m5x]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Monomastix_sp~~.~~_oke-1 Monomastix sp. oke-1]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA].~~	+

	==See Also==		==See Also==
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease\|Endonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012758074<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Monomastix sp. oke-1]]		[[Category: Monomastix sp. oke-1]]
	[[Category: Chevalier, B.]]		[[Category: Chevalier, B.]]

OCA at 09:13, 20 October 2012

OCA — Sat, 20 Oct 2012 09:13:22 GMT

←Older revision		Revision as of 09:13, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1m5x]] is a 4 chain structure ~~of [[Endonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Monomastix_sp. Monomastix sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA].	+	[[1m5x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Monomastix_sp._oke-1 Monomastix sp. oke-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5X OCA].

	==See Also==		==See Also==
Line 15:		Line 15:
	==Reference==		==Reference==
	<ref group="xtra">PMID:012758074</ref><references group="xtra"/>		<ref group="xtra">PMID:012758074</ref><references group="xtra"/>
-	[[Category: Monomastix sp.]]	+	[[Category: Monomastix sp. oke-1]]
	[[Category: Chevalier, B.]]		[[Category: Chevalier, B.]]
	[[Category: Lemieux, C.]]		[[Category: Lemieux, C.]]