1m9g - Revision history

OCA at 10:28, 16 August 2023

OCA — Wed, 16 Aug 2023 10:28:42 GMT

←Older revision		Revision as of 10:28, 16 August 2023
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	==Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI==		==Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI==
-	<StructureSection load='1m9g' size='340' side='right'caption='[[1m9g~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1m9g' size='340' side='right'caption='[[1m9g]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Diocu Diocu~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M9G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M9G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [https://pdbe.org/1m9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [https://www.ebi.ac.uk/pdbsum/1m9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9g ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [https://pdbe.org/1m9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [https://www.ebi.ac.uk/pdbsum/1m9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU]] Taste-modifying protein; intensely sweet-tasting protein.	+	[https://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Diocu~~]]	+	[[Category: Dioscoreophyllum cumminsii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Crescenzi, O]]	+	[[Category: Crescenzi O]]
-	[[Category: Picone, D]]	+	[[Category: Picone D]]
-	[[Category: Saviano, G]]	+	[[Category: Saviano G]]
-	[[Category: Spadaccini, R]]	+	[[Category: Spadaccini R]]
-	[[Category: Tancredi, T]]	+	[[Category: Tancredi T]]
-	[[Category: Temussi~~, P A~~]]	+	[[Category: Temussi PA]]
-	[[Category: Trabucco, F]]	+	[[Category: Trabucco F]]
-	~~[[Category: Stranded beta sheet 1 helix]]~~	+
-	~~[[Category: Plant protein~~]]	+

OCA at 05:58, 28 April 2021

OCA — Wed, 28 Apr 2021 05:58:52 GMT

←Older revision		Revision as of 05:58, 28 April 2021
Line 3:		Line 3:
	<StructureSection load='1m9g' size='340' side='right'caption='[[1m9g]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1m9g' size='340' side='right'caption='[[1m9g]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1M9G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M9G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [~~http~~://pdbe.org/1m9g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1m9g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9g ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [https://pdbe.org/1m9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [https://www.ebi.ac.uk/pdbsum/1m9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU]] Taste-modifying protein; intensely sweet-tasting protein.	+	[[https://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU]] Taste-modifying protein; intensely sweet-tasting protein.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:51, 24 July 2019

OCA — Wed, 24 Jul 2019 08:51:58 GMT

←Older revision		Revision as of 08:51, 24 July 2019
Line 1:		Line 1:

	==Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI==		==Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI==
-	<StructureSection load='1m9g' size='340' side='right' caption='[[1m9g]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1m9g' size='340' side='right'caption='[[1m9g]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>		<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Diocu]]		[[Category: Diocu]]
		+	[[Category: Large Structures]]
	[[Category: Crescenzi, O]]		[[Category: Crescenzi, O]]
	[[Category: Picone, D]]		[[Category: Picone, D]]

OCA at 10:31, 18 October 2017

OCA — Wed, 18 Oct 2017 10:31:17 GMT

←Older revision		Revision as of 10:31, 18 October 2017
Line 1:		Line 1:
		+
	==Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI==		==Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI==
	<StructureSection load='1m9g' size='340' side='right' caption='[[1m9g]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1m9g' size='340' side='right' caption='[[1m9g]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>		<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [http://pdbe.org/1m9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [http://www.ebi.ac.uk/pdbsum/1m9g PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [http://pdbe.org/1m9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [http://www.ebi.ac.uk/pdbsum/1m9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 05:10, 11 September 2015

OCA — Fri, 11 Sep 2015 05:10:47 GMT

←Older revision		Revision as of 05:10, 11 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>		<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [http://www.ebi.ac.uk/pdbsum/1m9g PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [http://pdbe.org/1m9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [http://www.ebi.ac.uk/pdbsum/1m9g PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1m9g" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:39, 25 December 2014

OCA — Thu, 25 Dec 2014 11:39:06 GMT

←Older revision		Revision as of 11:39, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>		<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [http://www.ebi.ac.uk/pdbsum/1m9g PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [http://www.ebi.ac.uk/pdbsum/1m9g PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU]] Taste-modifying protein; intensely sweet-tasting protein.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Diocu]]		[[Category: Diocu]]
-	[[Category: Crescenzi, O.]]	+	[[Category: Crescenzi, O]]
-	[[Category: Picone, D.]]	+	[[Category: Picone, D]]
-	[[Category: Saviano, G.]]	+	[[Category: Saviano, G]]
-	[[Category: Spadaccini, R.]]	+	[[Category: Spadaccini, R]]
-	[[Category: Tancredi, T.]]	+	[[Category: Tancredi, T]]
-	[[Category: Temussi, P A.]]	+	[[Category: Temussi, P A]]
-	[[Category: Trabucco, F.]]	+	[[Category: Trabucco, F]]
-	[[Category: ~~5 stranded~~ beta sheet 1 helix]]	+	[[Category: Stranded beta sheet 1 helix]]
	[[Category: Plant protein]]		[[Category: Plant protein]]

OCA at 05:23, 8 June 2014

OCA — Sun, 08 Jun 2014 05:23:37 GMT

←Older revision		Revision as of 05:23, 8 June 2014
Line 1:		Line 1:
-	[[~~Image~~:1m9g.~~png\|left\|200px~~]]	+	==Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI==
		+	<StructureSection load='1m9g' size='340' side='right' caption='[[1m9g]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [http://www.ebi.ac.uk/pdbsum/1m9g PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mechanism by which sweet proteins elicit a response on the T1R2-T1R3 sweet taste receptor is still mostly unknown but has been so far related to the presence of "sweet fingers" on the protein surface able to interact with the same mechanism as that of low molecular mass sweeteners. In the search for the identification of sweet fingers, we have solved the solution structure of G16A MNEI, a structural mutant that shows a reduction of one order of magnitude in sweetness with respect to its parent protein, MNEI, a single-chain monellin. Comparison of the structures of wild-type monellin and its G16A mutant shows that the mutation does not affect the structure of potential glucophores but produces a distortion of the surface owing to the partial relative displacement of elements of secondary structure. These results show conclusively that sweet proteins do not possess a sweet finger and strongly support the hypothesis that the mechanism of interaction of sweet-tasting proteins with the recently identified T1R2-T1R3 GPC receptor is different from that of low molecular mass sweeteners.

-	~~{{STRUCTURE_1m9g\| PDB=1m9g \| SCENE= }}~~	+	The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI.,Spadaccini R, Trabucco F, Saviano G, Picone D, Crescenzi O, Tancredi T, Temussi PA J Mol Biol. 2003 May 2;328(3):683-92. PMID:12706725<ref>PMID:12706725</ref>

-	~~===Solution structure of G16A-MNEI~~, a ~~structural mutant~~ of ~~single chain monellin MNEI===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12706725}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1m9g]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA].~~	+	</StructureSection>
-		+	[[Category: Diocu]]
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012706725<~~/~~ref~~><~~references group="xtra"~~/>	+
-	[[Category: ~~Dioscoreophyllum cumminsii~~]]	+
	[[Category: Crescenzi, O.]]		[[Category: Crescenzi, O.]]
	[[Category: Picone, D.]]		[[Category: Picone, D.]]

OCA: Protected "1m9g" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 17:07:14 GMT

Protected "1m9g" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:07, 14 August 2012

OCA at 17:07, 14 August 2012

OCA — Tue, 14 Aug 2012 17:07:13 GMT

←Older revision		Revision as of 17:07, 14 August 2012
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-	{{Seed}}
	[[Image:1m9g.png\|left\|200px]]		[[Image:1m9g.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1m9g\| PDB=1m9g \| SCENE= }}		{{STRUCTURE_1m9g\| PDB=1m9g \| SCENE= }}

	===Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI===		===Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12706725}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_12706725}}		{{ABSTRACT_PUBMED_12706725}}

	==About this Structure==		==About this Structure==
-	~~1M9G is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA].	+	[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA].

	==Reference==		==Reference==
-	The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI., Spadaccini R, Trabucco F, Saviano G, Picone D, Crescenzi O, Tancredi T, Temussi PA, J Mol Biol. 2003 May 2;328(3):683-92. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~12706725 12706725]~~	+	<ref group="xtra">PMID:012706725</ref><references group="xtra"/>
	[[Category: Dioscoreophyllum cumminsii]]		[[Category: Dioscoreophyllum cumminsii]]
-	[[Category: Single protein]]
	[[Category: Crescenzi, O.]]		[[Category: Crescenzi, O.]]
	[[Category: Picone, D.]]		[[Category: Picone, D.]]
Line 34:		Line 21:
	[[Category: Trabucco, F.]]		[[Category: Trabucco, F.]]
	[[Category: 5 stranded beta sheet 1 helix]]		[[Category: 5 stranded beta sheet 1 helix]]
-		+	[[Category: Plant protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Jul 2 23:29:26 2008''~~	+

OCA at 20:29, 2 July 2008

OCA — Wed, 02 Jul 2008 20:29:27 GMT

←Older revision		Revision as of 20:29, 2 July 2008
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-	[[Image:1m9g.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1m9g.png\|left\|200px]]

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	{{STRUCTURE_1m9g\| PDB=1m9g \| SCENE= }}		{{STRUCTURE_1m9g\| PDB=1m9g \| SCENE= }}

-	~~'''~~Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI~~'''~~	+	===Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI===


-	~~==Overview==~~	+	<!--
-	The ~~mechanism by which sweet proteins elicit a response on~~ the ~~T1R2-T1R3 sweet taste receptor is still mostly unknown but has been so far related~~ to the ~~presence of "sweet fingers"~~ on ~~the protein surface able to interact with the same mechanism as that of low molecular mass sweeteners~~. ~~In the search for the identification of sweet fingers~~, ~~we have solved~~ the ~~solution structure of G16A MNEI, a structural mutant that shows a reduction of one order of magnitude in sweetness with respect to its parent protein, MNEI, a single-chain monellin~~. ~~Comparison of the structures of wild~~-type monellin and its G16A mutant shows that the mutation does not affect the structure of potential glucophores but produces a distortion of the surface owing to the partial relative displacement of elements of secondary structure. These results show conclusively that sweet proteins do not possess a sweet finger and strongly support the hypothesis that the mechanism of interaction of sweet-~~tasting proteins with the recently identified T1R2-T1R3 GPC receptor is different from that of low molecular mass sweeteners.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_12706725}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 12706725 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_12706725}}

	==About this Structure==		==About this Structure==
-	1M9G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA].	+	1M9G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA].

	==Reference==		==Reference==
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	[[Category: Trabucco, F.]]		[[Category: Trabucco, F.]]
	[[Category: 5 stranded beta sheet 1 helix]]		[[Category: 5 stranded beta sheet 1 helix]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May~~ ~~3 00~~:47:24 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:29:26 2008''