1mb9 - Revision history

OCA at 07:42, 14 February 2024

OCA — Wed, 14 Feb 2024 07:42:38 GMT

←Older revision		Revision as of 07:42, 14 February 2024
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	<StructureSection load='1mb9' size='340' side='right'caption='[[1mb9]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='1mb9' size='340' side='right'caption='[[1mb9]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mb9]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1611 As 4.1611~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1MB9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mb9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MB9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.11Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jgt\|1jgt]], [[1mbz\|1mbz]], [[1mc1\|1mc1]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 AS 4.1611])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [https://pdbe.org/1mb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [https://www.ebi.ac.uk/pdbsum/1mb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mb9 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [~~http~~://pdbe.org/1mb9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mb9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mb9 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BLS_STRCL BLS_STRCL]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mb9 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mb9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The catalytic cycle of the ATP/Mg(2+)-dependent enzyme beta-lactam synthetase (beta-LS) from Streptomyces clavuligerus has been observed through a series of x-ray crystallographic snapshots. Chemistry is initiated by the ordered binding of ATP/Mg(2+) and N(2)-(carboxyethyl)-l-arginine (CEA) to the apoenzyme. The apo and ATP/Mg(2+) structures described here, along with the previously described CEA.alpha,beta-methyleneadenosine 5'-triphosphate (CEA.AMP-CPP)/Mg(2+) structure, illuminate changes in active site geometry that favor adenylation. In addition, an acyladenylate intermediate has been trapped. The substrate analog N(2)-(carboxymethyl)-l-arginine (CMA) was adenylated by ATP in the crystal and represents a close structural analog of the previously proposed CEA-adenylate intermediate. Finally, the structure of the ternary product complex deoxyguanidinoproclavaminic acid (DGPC).AMP/PP(i)/Mg(2+) has been determined. The CMA-AMP/PP(i)/Mg(2+) and DGPC.AMP/PP(i)/Mg(2+) structures reveal interactions in the active site that facilitate beta-lactam formation. All of the ATP-bound structures differ from the previously described CEA.AMP-CPP/Mg(2+) structure in that two Mg(2+) ions are found in the active sites. These Mg(2+) ions play critical roles in both the adenylation and beta-lactamization reactions.
-
-	The catalytic cycle of beta -lactam synthetase observed by x-ray crystallographic snapshots.,Miller MT, Bachmann BO, Townsend CA, Rosenzweig AC Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14752-7. Epub 2002 Oct 30. PMID:12409610<ref>PMID:12409610</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1mb9" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1611]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bachmann, B O~~]]	+	[[Category: Streptomyces clavuligerus]]
-	[[Category: ~~Miller, M T~~]]	+	[[Category: Bachmann BO]]
-	[[Category: ~~Rosenzweig, A C~~]]	+	[[Category: Miller MT]]
-	[[Category: ~~Townsend, C A~~]]	+	[[Category: Rosenzweig AC]]
-	[[Category: ~~Asparagine synthetase]]~~	+	[[Category: Townsend CA]]
-	~~[[Category: Beta-lactam synthetase]]~~	+
-	~~[[Category: Carboxyethyl arginine]]~~	+
-	~~[[Category: Clavulanic acid]]~~	+
-	~~[[Category: Deoxyguanidinoproclavaminic acid]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 08:35, 2 December 2020

OCA — Wed, 02 Dec 2020 08:35:51 GMT

←Older revision		Revision as of 08:35, 2 December 2020
Line 1:		Line 1:

	==BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP==		==BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP==
-	<StructureSection load='1mb9' size='340' side='right' caption='[[1mb9]], [[Resolution\|resolution]] 2.11Å' scene=''>	+	<StructureSection load='1mb9' size='340' side='right'caption='[[1mb9]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mb9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MB9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mb9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1MB9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jgt\|1jgt]], [[1mbz\|1mbz]], [[1mc1\|1mc1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jgt\|1jgt]], [[1mbz\|1mbz]], [[1mc1\|1mc1]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 AS 4.1611])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 AS 4.1611])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [http://pdbe.org/1mb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mb9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mb9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [http://pdbe.org/1mb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mb9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mb9 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mb/1mb9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mb/1mb9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1611]]		[[Category: As 4 1611]]
		+	[[Category: Large Structures]]
	[[Category: Bachmann, B O]]		[[Category: Bachmann, B O]]
	[[Category: Miller, M T]]		[[Category: Miller, M T]]

OCA at 14:41, 12 October 2017

OCA — Thu, 12 Oct 2017 14:41:20 GMT

←Older revision		Revision as of 14:41, 12 October 2017
Line 1:		Line 1:
		+
	==BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP==		==BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP==
	<StructureSection load='1mb9' size='340' side='right' caption='[[1mb9]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='1mb9' size='340' side='right' caption='[[1mb9]], [[Resolution\|resolution]] 2.11Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jgt\|1jgt]], [[1mbz\|1mbz]], [[1mc1\|1mc1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jgt\|1jgt]], [[1mbz\|1mbz]], [[1mc1\|1mc1]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 AS 4.1611])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 AS 4.1611])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [http://pdbe.org/1mb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mb9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [http://pdbe.org/1mb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mb9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mb9 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 15:59, 7 February 2016

OCA — Sun, 07 Feb 2016 15:59:12 GMT

←Older revision		Revision as of 15:59, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mb9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:01, 10 September 2015

OCA — Thu, 10 Sep 2015 03:01:19 GMT

←Older revision		Revision as of 03:01, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1mb9' size='340' side='right' caption='[[1mb9]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='1mb9' size='340' side='right' caption='[[1mb9]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mb9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_clavuligerus Streptomyces clavuligerus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MB9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mb9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MB9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jgt\|1jgt]], [[1mbz\|1mbz]], [[1mc1\|1mc1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jgt\|1jgt]], [[1mbz\|1mbz]], [[1mc1\|1mc1]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 ~~Streptomyces clavuligerus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 AS 4.1611])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mb9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [http://pdbe.org/1mb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mb9 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mb9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces clavuligerus~~]]	+	[[Category: As 4 1611]]
	[[Category: Bachmann, B O]]		[[Category: Bachmann, B O]]
	[[Category: Miller, M T]]		[[Category: Miller, M T]]

OCA at 16:15, 5 January 2015

OCA — Mon, 05 Jan 2015 16:15:46 GMT

←Older revision		Revision as of 16:15, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mb9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MB9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mb9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MB9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jgt\|1jgt]], [[1mbz\|1mbz]], [[1mc1\|1mc1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jgt\|1jgt]], [[1mbz\|1mbz]], [[1mc1\|1mc1]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 Streptomyces clavuligerus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 Streptomyces clavuligerus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mb9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mb9 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces clavuligerus]]		[[Category: Streptomyces clavuligerus]]
-	[[Category: Bachmann, B O.]]	+	[[Category: Bachmann, B O]]
-	[[Category: Miller, M T.]]	+	[[Category: Miller, M T]]
-	[[Category: Rosenzweig, A C.]]	+	[[Category: Rosenzweig, A C]]
-	[[Category: Townsend, C A.]]	+	[[Category: Townsend, C A]]
	[[Category: Asparagine synthetase]]		[[Category: Asparagine synthetase]]
	[[Category: Beta-lactam synthetase]]		[[Category: Beta-lactam synthetase]]

OCA at 17:32, 28 September 2014

OCA — Sun, 28 Sep 2014 17:32:42 GMT

←Older revision		Revision as of 17:32, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1mb9.~~png~~\|~~left~~\|200px]]	+	==BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP==
		+	<StructureSection load='1mb9' size='340' side='right' caption='[[1mb9]], [[Resolution\|resolution]] 2.11Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mb9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MB9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jgt\|1jgt]], [[1mbz\|1mbz]], [[1mc1\|1mc1]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">1901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 Streptomyces clavuligerus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mb9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mb9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mb/1mb9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The catalytic cycle of the ATP/Mg(2+)-dependent enzyme beta-lactam synthetase (beta-LS) from Streptomyces clavuligerus has been observed through a series of x-ray crystallographic snapshots. Chemistry is initiated by the ordered binding of ATP/Mg(2+) and N(2)-(carboxyethyl)-l-arginine (CEA) to the apoenzyme. The apo and ATP/Mg(2+) structures described here, along with the previously described CEA.alpha,beta-methyleneadenosine 5'-triphosphate (CEA.AMP-CPP)/Mg(2+) structure, illuminate changes in active site geometry that favor adenylation. In addition, an acyladenylate intermediate has been trapped. The substrate analog N(2)-(carboxymethyl)-l-arginine (CMA) was adenylated by ATP in the crystal and represents a close structural analog of the previously proposed CEA-adenylate intermediate. Finally, the structure of the ternary product complex deoxyguanidinoproclavaminic acid (DGPC).AMP/PP(i)/Mg(2+) has been determined. The CMA-AMP/PP(i)/Mg(2+) and DGPC.AMP/PP(i)/Mg(2+) structures reveal interactions in the active site that facilitate beta-lactam formation. All of the ATP-bound structures differ from the previously described CEA.AMP-CPP/Mg(2+) structure in that two Mg(2+) ions are found in the active sites. These Mg(2+) ions play critical roles in both the adenylation and beta-lactamization reactions.

-	~~{{STRUCTURE_1mb9\| PDB=1mb9 \| SCENE= }}~~	+	The catalytic cycle of beta -lactam synthetase observed by x-ray crystallographic snapshots.,Miller MT, Bachmann BO, Townsend CA, Rosenzweig AC Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14752-7. Epub 2002 Oct 30. PMID:12409610<ref>PMID:12409610</ref>

-	~~===BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12409610}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1mb9]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012409610<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces clavuligerus]]		[[Category: Streptomyces clavuligerus]]
	[[Category: Bachmann, B O.]]		[[Category: Bachmann, B O.]]

OCA: Protected "1mb9" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 20:29:35 GMT

Protected "1mb9" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:29, 5 December 2012

OCA at 20:29, 5 December 2012

OCA — Wed, 05 Dec 2012 20:29:28 GMT

←Older revision		Revision as of 20:29, 5 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1mb9.png\|left\|200px]]		[[Image:1mb9.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1mb9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1mb9\| PDB=1mb9 \| SCENE= }}		{{STRUCTURE_1mb9\| PDB=1mb9 \| SCENE= }}

	===BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP===		===BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12409610}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12409610 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12409610}}		{{ABSTRACT_PUBMED_12409610}}

	==About this Structure==		==About this Structure==
-	~~1MB9~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA].	+	[[1mb9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12409610~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012409610</ref><references group="xtra"/>
	[[Category: Streptomyces clavuligerus]]		[[Category: Streptomyces clavuligerus]]
	[[Category: Bachmann, B O.]]		[[Category: Bachmann, B O.]]
Line 34:		Line 22:
	[[Category: Clavulanic acid]]		[[Category: Clavulanic acid]]
	[[Category: Deoxyguanidinoproclavaminic acid]]		[[Category: Deoxyguanidinoproclavaminic acid]]
-		+	[[Category: Hydrolase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 14:56:58 2009''~~	+

OCA at 12:57, 16 February 2009

OCA — Mon, 16 Feb 2009 12:57:00 GMT

←Older revision		Revision as of 12:57, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1MB9 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA].	+	1MB9 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB9 OCA].

	==Reference==		==Reference==
-	The catalytic cycle of beta -lactam synthetase observed by x-ray crystallographic snapshots., Miller MT, Bachmann BO, Townsend CA, Rosenzweig AC, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14752-7. Epub 2002 Oct 30. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/12409610 12409610]~~	+	<ref group="xtra">PMID:12409610</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Streptomyces clavuligerus]]		[[Category: Streptomyces clavuligerus]]
	[[Category: Bachmann, B O.]]		[[Category: Bachmann, B O.]]
Line 36:		Line 35:
	[[Category: Deoxyguanidinoproclavaminic acid]]		[[Category: Deoxyguanidinoproclavaminic acid]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 2 23~~:36:~~42 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:56:58 2009''