1mi4 - Revision history

OCA at 07:18, 25 October 2023

OCA — Wed, 25 Oct 2023 07:18:49 GMT

←Older revision		Revision as of 07:18, 25 October 2023
Line 3:		Line 3:
	<StructureSection load='1mi4' size='340' side='right'caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1mi4' size='340' side='right'caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>~~, ~~<scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1g6s\|1g6s]], [[1g6t\|1g6t]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>aroA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/3-phosphoshikimate_1~~-~~carboxyvinyltransferase~~ 3-~~phosphoshikimate 1-carboxyvinyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [https://pdbe.org/1mi4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [https://www.ebi.ac.uk/pdbsum/1mi4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [https://pdbe.org/1mi4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [https://www.ebi.ac.uk/pdbsum/1mi4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AROA_ECOLI AROA_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[EPSP synthase\|EPSP synthase]]	+	*[[EPSP synthase 3D structures\|EPSP synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eschenburg, S]]	+	[[Category: Eschenburg S]]
-	[[Category: Healy~~, M L~~]]	+	[[Category: Healy ML]]
-	[[Category: Lushington~~, G H~~]]	+	[[Category: Lushington GH]]
-	[[Category: Priestman~~, M A~~]]	+	[[Category: Priestman MA]]
-	[[Category: Schonbrunn, E]]	+	[[Category: Schonbrunn E]]
-	~~[[Category: Inside-out alpha-beta barrel]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:25, 10 November 2021

OCA — Wed, 10 Nov 2021 06:25:47 GMT

←Older revision		Revision as of 06:25, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='1mi4' size='340' side='right'caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1mi4' size='340' side='right'caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g6s\|1g6s]], [[1g6t\|1g6t]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g6s\|1g6s]], [[1g6t\|1g6t]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [~~http~~://pdbe.org/1mi4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mi4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [https://pdbe.org/1mi4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [https://www.ebi.ac.uk/pdbsum/1mi4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi4 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:37, 2 December 2020

OCA — Wed, 02 Dec 2020 08:37:24 GMT

←Older revision		Revision as of 08:37, 2 December 2020
Line 1:		Line 1:

	==Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate==		==Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate==
-	<StructureSection load='1mi4' size='340' side='right' caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='1mi4' size='340' side='right'caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g6s\|1g6s]], [[1g6t\|1g6t]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g6s\|1g6s]], [[1g6t\|1g6t]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [http://pdbe.org/1mi4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [http://pdbe.org/1mi4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi4 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mi/1mi4_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mi/1mi4_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1mi4" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1mi4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[EPSP synthase\|EPSP synthase]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]		[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Eschenburg, S]]		[[Category: Eschenburg, S]]
	[[Category: Healy, M L]]		[[Category: Healy, M L]]

OCA at 08:38, 12 October 2017

OCA — Thu, 12 Oct 2017 08:38:12 GMT

←Older revision		Revision as of 08:38, 12 October 2017
Line 1:		Line 1:
		+
	==Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate==		==Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate==
	<StructureSection load='1mi4' size='340' side='right' caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1mi4' size='340' side='right' caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [http://pdbe.org/1mi4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [http://pdbe.org/1mi4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi4 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1mi4" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1mi4" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[EPSP synthase\|EPSP synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:35, 8 February 2016

OCA — Mon, 08 Feb 2016 16:35:01 GMT

←Older revision		Revision as of 16:35, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mi4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:27, 10 September 2015

OCA — Thu, 10 Sep 2015 13:27:10 GMT

←Older revision		Revision as of 13:27, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1mi4' size='340' side='right' caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1mi4' size='340' side='right' caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g6s\|1g6s]], [[1g6t\|1g6t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g6s\|1g6s]], [[1g6t\|1g6t]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [http://pdbe.org/1mi4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi4 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mi4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]		[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
-	[[Category: Escherichia coli]]
	[[Category: Eschenburg, S]]		[[Category: Eschenburg, S]]
	[[Category: Healy, M L]]		[[Category: Healy, M L]]

OCA at 15:38, 5 January 2015

OCA — Mon, 05 Jan 2015 15:38:27 GMT

←Older revision		Revision as of 15:38, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g6s\|1g6s]], [[1g6t\|1g6t]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g6s\|1g6s]], [[1g6t\|1g6t]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi4 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]		[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Eschenburg, S.]]	+	[[Category: Eschenburg, S]]
-	[[Category: Healy, M L.]]	+	[[Category: Healy, M L]]
-	[[Category: Lushington, G H.]]	+	[[Category: Lushington, G H]]
-	[[Category: Priestman, M A.]]	+	[[Category: Priestman, M A]]
-	[[Category: Schonbrunn, E.]]	+	[[Category: Schonbrunn, E]]
	[[Category: Inside-out alpha-beta barrel]]		[[Category: Inside-out alpha-beta barrel]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 15:31, 28 September 2014

OCA — Sun, 28 Sep 2014 15:31:40 GMT

←Older revision		Revision as of 15:31, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1mi4.~~png~~\|~~left~~\|200px]]	+	==Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate==
		+	<StructureSection load='1mi4' size='340' side='right' caption='[[1mi4]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mi4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MI4 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g6s\|1g6s]], [[1g6t\|1g6t]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mi4 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi4 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mi/1mi4_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The enzyme 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase (EC 2.5.1.19) is essential for the biosynthesis of aromatic compounds in plants and microbes and is the unique target of the herbicide glyphosate. One of the first glyphosate-insensitive enzymes reported was a Gly96Ala mutant of EPSP synthase from Klebsiella pneumoniae. We have introduced this single-site mutation into the highly homologous EPSP synthase from Escherichia coli. The mutant enzyme is insensitive to glyphosate with unaltered affinity for its first substrate, shikimate-3-phosphate (S3P), but displays a 30-fold lower affinity for its second substrate, phosphoenolpyruvate (PEP). Using X-ray crystallography, we solved the structure of Gly96Ala-EPSP synthase liganded with S3P to 0.17 nm resolution. The crystal structure shows that the additional methyl group from Ala96 protrudes into the active site of the enzyme. While the interactions between enzyme and S3P remain unaffected, the accessible volume for glyphosate binding is substantially reduced. Exploiting the crystallographic results for molecular modeling, we demonstrate that PEP but not glyphosate can be docked in the Gly96Ala-modified binding site. The predicted PEP binding site satisfies the earlier proposed interaction pattern for PEP with EPSP synthase and corroborates the assumption that glyphosate and PEP target the same binding site.

-	~~{{STRUCTURE_1mi4\| PDB=1mi4 \| SCENE= }}~~	+	How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli.,Eschenburg S, Healy ML, Priestman MA, Lushington GH, Schonbrunn E Planta. 2002 Nov;216(1):129-35. Epub 2002 Nov 12. PMID:12430021<ref>PMID:12430021</ref>

-	~~===Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_12430021}}~~	+	==See Also==
-		+	*[[EPSP synthase\|EPSP synthase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1mi4~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:012430021<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]		[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA: Protected "1mi4" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 19:54:08 GMT

Protected "1mi4" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:54, 5 December 2012

OCA at 19:54, 5 December 2012

OCA — Wed, 05 Dec 2012 19:54:06 GMT

←Older revision		Revision as of 19:54, 5 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1mi4.png\|left\|200px]]		[[Image:1mi4.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1mi4", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1mi4\| PDB=1mi4 \| SCENE= }}		{{STRUCTURE_1mi4\| PDB=1mi4 \| SCENE= }}

	===Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate===		===Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12430021}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12430021 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12430021}}		{{ABSTRACT_PUBMED_12430021}}

	==About this Structure==		==About this Structure==
-	~~1MI4~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA].	+	[[1mi4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12430021~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012430021</ref><references group="xtra"/>
	[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]		[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
Line 32:		Line 20:
	[[Category: Schonbrunn, E.]]		[[Category: Schonbrunn, E.]]
	[[Category: Inside-out alpha-beta barrel]]		[[Category: Inside-out alpha-beta barrel]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 00:29:14 2009''~~	+