1mii - Revision history

OCA at 18:50, 29 November 2023

OCA — Wed, 29 Nov 2023 18:50:13 GMT

←Older revision		Revision as of 18:50, 29 November 2023
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	==SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII==		==SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII==
-	<StructureSection load='1mii' size='340' side='right'caption='[[1mii~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1mii' size='340' side='right'caption='[[1mii]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mii]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MII FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mii]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_magus Conus magus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MII FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=NH2:AMINO+GROUP'>NH2</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [https://pdbe.org/1mii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [https://www.ebi.ac.uk/pdbsum/1mii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mii ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [https://pdbe.org/1mii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [https://www.ebi.ac.uk/pdbsum/1mii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mii ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~CA2_CONMA CA2_CONMA]~~] Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This toxin blocks neuronal mammalian nAChRs (alpha-6 or -3/beta-2 or -3 > alpha-3/beta-2 > alpha-3/beta-4 = alpha-4/beta-2).<ref>PMID:11044728</ref> <ref>PMID:8631783</ref>	+	[https://www.uniprot.org/uniprot/CA12_CONMA CA12_CONMA] Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This toxin blocks neuronal mammalian nAChRs (alpha-6/alpha-3-beta-2-beta-3 (0.39 nM) > alpha-3-beta-2/CHRNA3-CHRNB2 > alpha-3-beta-4/CHRNA3-CHRNB4 = alpha-4-beta-2/CHRNA4-CHRNB2) (PubMed:15005608, PubMed:20145249). Also exhibits inhibition of D.melanogaster alpha-7/CHRNA7 nAChRs (PubMed:25466886). In addition, inhibits alpha-6/alpha-3-beta-4 (CHRNA6/CHRNA3-CHRNB4) nAChR with a higher potency on human (IC(50)=1.49 nM) than on rat receptors (IC(50)=31.5 nM) (PubMed:33523678). Its binding to alpha-3-beta-2/CHRNA3-CHRNB2 nAChR is prevented by alpha-conotoxin Lt1a, suggesting that the two toxins have overlapping binding sites (PubMed:20145249). In addition, both toxins have distinct nAChR binding mode (PubMed:20145249). In vivo, inhibits Ehrlich carcinoma growth and increase mouse survival (PubMed:32272633). These effects are greatly enhanced when the toxin is applied with the non-selective cyclooxygenase inhibitor indomethacin (PubMed:32272633).<ref>PMID:11044728</ref> <ref>PMID:15005608</ref> <ref>PMID:15044624</ref> <ref>PMID:15929983</ref> <ref>PMID:16964981</ref> <ref>PMID:20145249</ref> <ref>PMID:25466886</ref> <ref>PMID:32272633</ref> <ref>PMID:33523678</ref> <ref>PMID:8631783</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Conus magus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lysozyme]]~~	+	[[Category: Alewood PF]]
-	[[Category: Alewood~~, P F~~]]	+	[[Category: Bingham JP]]
-	[[Category: Bingham~~, J P~~]]	+	[[Category: Craik DJ]]
-	[[Category: Craik~~, D J~~]]	+	[[Category: Hill JM]]
-	[[Category: Hill~~, J M~~]]	+	[[Category: Miranda LP]]
-	[[Category: Miranda~~, L P~~]]	+	[[Category: Oomen CJ]]
-	[[Category: Oomen~~, C J]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Neurotoxin]]~~	+
-	~~[[Category: Nicotinic acetylcholine receptor~~]]	+

OCA at 09:27, 5 May 2021

OCA — Wed, 05 May 2021 09:27:22 GMT

←Older revision		Revision as of 09:27, 5 May 2021
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	<StructureSection load='1mii' size='340' side='right'caption='[[1mii]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1mii' size='340' side='right'caption='[[1mii]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mii]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MII FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mii]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MII FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [~~http~~://pdbe.org/1mii PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mii PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mii ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [https://pdbe.org/1mii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [https://www.ebi.ac.uk/pdbsum/1mii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mii ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CA2_CONMA CA2_CONMA]] Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This toxin blocks neuronal mammalian nAChRs (alpha-6 or -3/beta-2 or -3 > alpha-3/beta-2 > alpha-3/beta-4 = alpha-4/beta-2).<ref>PMID:11044728</ref> <ref>PMID:8631783</ref>	+	[[https://www.uniprot.org/uniprot/CA2_CONMA CA2_CONMA]] Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This toxin blocks neuronal mammalian nAChRs (alpha-6 or -3/beta-2 or -3 > alpha-3/beta-2 > alpha-3/beta-4 = alpha-4/beta-2).<ref>PMID:11044728</ref> <ref>PMID:8631783</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:42, 31 July 2019

OCA — Wed, 31 Jul 2019 07:42:27 GMT

←Older revision		Revision as of 07:42, 31 July 2019
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII==		==SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII==
-	<StructureSection load='1mii' size='340' side='right' caption='[[1mii]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1mii' size='340' side='right'caption='[[1mii]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mii]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MII FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mii]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MII FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Alewood, P F]]		[[Category: Alewood, P F]]

OCA at 10:07, 25 October 2017

OCA — Wed, 25 Oct 2017 10:07:30 GMT

←Older revision		Revision as of 10:07, 25 October 2017
Line 1:		Line 1:
		+
	==SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII==		==SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII==
	<StructureSection load='1mii' size='340' side='right' caption='[[1mii]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1mii' size='340' side='right' caption='[[1mii]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [http://pdbe.org/1mii PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [http://www.ebi.ac.uk/pdbsum/1mii PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [http://pdbe.org/1mii PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [http://www.ebi.ac.uk/pdbsum/1mii PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mii ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 05:44, 10 September 2015

OCA — Thu, 10 Sep 2015 05:44:31 GMT

←Older revision		Revision as of 05:44, 10 September 2015
Line 5:		Line 5:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [http://www.ebi.ac.uk/pdbsum/1mii PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [http://pdbe.org/1mii PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [http://www.ebi.ac.uk/pdbsum/1mii PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~CXA2_CONMA CXA2_CONMA~~]] Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This toxin blocks neuronal mammalian nAChRs (alpha-6 or -3/beta-2 or -3 > alpha-3/beta-2 > alpha-3/beta-4 = alpha-4/beta-2).<ref>PMID:~~8631783~~</ref> <ref>PMID:~~11044728~~</ref>	+	[[http://www.uniprot.org/uniprot/CA2_CONMA CA2_CONMA]] Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This toxin blocks neuronal mammalian nAChRs (alpha-6 or -3/beta-2 or -3 > alpha-3/beta-2 > alpha-3/beta-4 = alpha-4/beta-2).<ref>PMID:11044728</ref> <ref>PMID:8631783</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mii" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:51, 24 December 2014

OCA — Wed, 24 Dec 2014 18:51:16 GMT

←Older revision		Revision as of 18:51, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mii]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MII FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mii]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MII FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [http://www.ebi.ac.uk/pdbsum/1mii PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [http://www.ebi.ac.uk/pdbsum/1mii PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CXA2_CONMA CXA2_CONMA]] Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This toxin blocks neuronal mammalian nAChRs (alpha-6 or -3/beta-2 or -3 > alpha-3/beta-2 > alpha-3/beta-4 = alpha-4/beta-2).<ref>PMID:8631783</ref> <ref>PMID:11044728</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
-	[[Category: Alewood, P F.]]	+	[[Category: Alewood, P F]]
-	[[Category: Bingham, J P.]]	+	[[Category: Bingham, J P]]
-	[[Category: Craik, D J.]]	+	[[Category: Craik, D J]]
-	[[Category: Hill, J M.]]	+	[[Category: Hill, J M]]
-	[[Category: Miranda, L P.]]	+	[[Category: Miranda, L P]]
-	[[Category: Oomen, C J.]]	+	[[Category: Oomen, C J]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
	[[Category: Nicotinic acetylcholine receptor]]		[[Category: Nicotinic acetylcholine receptor]]

OCA at 14:33, 28 September 2014

OCA — Sun, 28 Sep 2014 14:33:04 GMT

←Older revision		Revision as of 14:33, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1mii.~~png~~\|~~left\|200px~~]]	+	==SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII==
		+	<StructureSection load='1mii' size='340' side='right' caption='[[1mii]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mii]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MII FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mii OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mii RCSB], [http://www.ebi.ac.uk/pdbsum/1mii PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	alpha-Conotoxin MII, a 16-residue polypeptide from the venom of the piscivorous cone snail Conus magus, is a potent and highly specific blocker of mammalian neuronal nicotinic acetylcholine receptors composed of alpha3 beta2 subunits. The role of this receptor type in the modulation of neurotransmitter release and its relevance to the problems of addiction and psychosis emphasize the importance of a structural understanding of the mode of interaction of MII with the alpha3 beta2 interface. Here we describe the three-dimensional solution structure of MII determined using 2D 1H NMR spectroscopy. Structural restraints consisting of 376 interproton distances inferred from NOEs and 12 dihedral restraints derived from spin-spin coupling constants were used as input for simulated annealing calculations and energy minimization in the program X-PLOR. The final set of 20 structures is exceptionally well-defined with mean pairwise rms differences over the whole molecule of 0.07 A for the backbone atoms and 0.34 A for all heavy atoms. MII adopts a compact structure incorporating a central segment of alpha-helix and beta-turns at the N- and C-termini. The molecule is stabilized by two disulfide bonds, which provide cross-links between the N-terminus and both the middle and C-terminus of the structure. The susceptibility of the structure to conformational change was examined using several different solvent conditions. While the global fold of MII remains the same, the structure is stabilized in a more hydrophobic environment provided by the addition of acetonitrile or trifluoroethanol to the aqueous solution. The distribution of amino acid side chains in MII creates distinct hydrophobic and polar patches on its surface that may be important for the specific interaction with the alpha3beta2 neuronal nAChR. A comparison of the structure of MII with other neuronal-specific alpha-conotoxins provides insights into their mode of interaction with these receptors.

-	~~{{STRUCTURE_1mii\| PDB=1mii \| SCENE= }}~~	+	Three-dimensional solution structure of alpha-conotoxin MII by NMR spectroscopy: effects of solution environment on helicity.,Hill JM, Oomen CJ, Miranda LP, Bingham JP, Alewood PF, Craik DJ Biochemistry. 1998 Nov 10;37(45):15621-30. PMID:9843366<ref>PMID:9843366</ref>

-	~~===SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9843366}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1mii]] is a 1 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA].~~	+	</StructureSection>
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Alewood, P F.]]		[[Category: Alewood, P F.]]

OCA: Protected "1mii" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 20:06:57 GMT

Protected "1mii" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:06, 5 December 2012

OCA at 20:06, 5 December 2012

OCA — Wed, 05 Dec 2012 20:06:54 GMT

←Older revision		Revision as of 20:06, 5 December 2012
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-	{{Seed}}
	[[Image:1mii.png\|left\|200px]]		[[Image:1mii.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1mii", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1mii\| PDB=1mii \| SCENE= }}		{{STRUCTURE_1mii\| PDB=1mii \| SCENE= }}

	===SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII===		===SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9843366}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9843366 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_9843366}}		{{ABSTRACT_PUBMED_9843366}}

	==About this Structure==		==About this Structure==
-	~~1MII is a~~ [[~~Single protein~~]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA].	+	[[1mii]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA].
-		+
-	~~==Reference==~~	+
-	Three-dimensional solution structure of alpha-conotoxin MII by NMR spectroscopy: effects of solution environment on helicity., Hill JM, Oomen CJ, Miranda LP, Bingham JP, Alewood PF, Craik DJ, Biochemistry. 1998 Nov 10;37(45):15621-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9843366 9843366]	+
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
-	[[Category: Single protein]]
	[[Category: Alewood, P F.]]		[[Category: Alewood, P F.]]
	[[Category: Bingham, J P.]]		[[Category: Bingham, J P.]]
Line 32:		Line 16:
	[[Category: Miranda, L P.]]		[[Category: Miranda, L P.]]
	[[Category: Oomen, C J.]]		[[Category: Oomen, C J.]]
		+	[[Category: Hydrolase]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
	[[Category: Nicotinic acetylcholine receptor]]		[[Category: Nicotinic acetylcholine receptor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:02:42 2008''

OCA at 21:02, 2 July 2008

OCA — Wed, 02 Jul 2008 21:02:45 GMT

←Older revision		Revision as of 21:02, 2 July 2008
Line 1:		Line 1:
-	[[Image:1mii.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1mii.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1mii\| PDB=1mii \| SCENE= }}		{{STRUCTURE_1mii\| PDB=1mii \| SCENE= }}

-	~~'''~~SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII~~'''~~	+	===SOLUTION STRUCTURE OF ALPHA-CONOTOXIN MII===


-	~~==Overview==~~	+	<!--
-	~~alpha~~-~~Conotoxin MII, a 16~~-residue polypeptide from the venom of the piscivorous cone snail Conus magus, is a potent and highly specific blocker of mammalian neuronal nicotinic acetylcholine receptors composed of alpha3 beta2 subunits. The ~~role of~~ this ~~receptor type in~~ the ~~modulation of neurotransmitter release and its relevance~~ to the problems of addiction and psychosis emphasize the importance of a structural understanding of the mode of interaction of MII with the alpha3 beta2 interface. Here we describe the three-dimensional solution structure of MII determined using 2D 1H NMR spectroscopy. Structural restraints consisting of 376 interproton distances inferred from NOEs and 12 dihedral restraints derived from spin-spin coupling constants were used as input for simulated annealing calculations and energy minimization in the program X-PLOR. The final set of 20 structures is exceptionally well-defined with mean pairwise rms differences over the whole molecule of 0.07 A for the backbone atoms and 0.34 A for all heavy atoms. MII adopts a compact structure incorporating a central segment of alpha-helix and beta-turns at ~~the N- and C-termini~~. ~~The molecule is stabilized by two disulfide bonds, which provide cross-links between the N-terminus and both the middle and C-terminus of the structure~~. ~~The susceptibility of the structure to conformational change was examined using several different solvent conditions. While the global fold of MII remains the same~~, ~~the structure~~ is ~~stabilized in a more hydrophobic environment provided by~~ the ~~addition of acetonitrile or trifluoroethanol to the aqueous solution~~. The distribution of amino acid side chains in MII creates distinct hydrophobic and polar patches on its surface that may be important for the specific interaction with the alpha3beta2 neuronal nAChR. A comparison of the structure of MII with other neuronal-~~specific alpha~~-~~conotoxins provides insights into their mode of interaction with these receptors.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_9843366}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9843366 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9843366}}

	==About this Structure==		==About this Structure==
-	1MII is a [[Single protein]] structure. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA].	+	1MII is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MII OCA].

	==Reference==		==Reference==
Line 30:		Line 34:
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
	[[Category: Nicotinic acetylcholine receptor]]		[[Category: Nicotinic acetylcholine receptor]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May~~ 3 01:06:39 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:02:42 2008''