1mlb - Revision history

OCA at 08:37, 10 April 2024

OCA — Wed, 10 Apr 2024 08:37:56 GMT

←Older revision		Revision as of 08:37, 10 April 2024
Line 3:		Line 3:
	<StructureSection load='1mlb' size='340' side='right'caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1mlb' size='340' side='right'caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [https://pdbe.org/1mlb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [https://www.ebi.ac.uk/pdbsum/1mlb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlb ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [https://pdbe.org/1mlb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [https://www.ebi.ac.uk/pdbsum/1mlb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlb ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mlb ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mlb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The three-dimensional structures of the free and antigen-complexed Fabs from the mouse monoclonal anti-hen egg white lysozyme antibody D44.1 have been solved and refined by X-ray crystallographic techniques. The crystals of the free and lysozyme-bound Fabs were grown under identical conditions and their X-ray diffraction data were collected to 2.1 and 2.5 A, respectively. Two molecules of the Fab-lysozyme complex in the asymmetric unit of the crystals show nearly identical conformations and thus confirm the essential structural features of the antigen-antibody interface. Three buried water molecules enhance the surface complementarity at the interface and provide hydrogen bonds to stabilize the complex. Two hydrophobic buried holes are present at the interface which, although large enough to accommodate solvent molecules, are void. The combining site residues of the complexed FabD44.1 exhibit reduced temperature factors compared with those of the free Fab. Furthermore, small perturbations in atomic positions and rearrangements of side-chains at the combining site, and a relative rearrangement of the variable domains of the light (VL) and the heavy (VH) chains, detail a Fab accommodation of the bound lysozyme. The amino acid sequence of the VH domain, as well as the epitope of lysozyme recognized by D44.1 are very close to those previously reported for the monoclonal antibody HyHEL-5. A feature central to the FabD44.1 and FabHyHEL-5 complexes with lysozyme are three salt bridges between VH glutamate residues 35 and 50 and lysozyme arginine residues 45 and 68. The presence of the three salt bridges in the D44.1-lysozyme interface indicates that these bonds are not responsible for the 1000-fold increase in affinity for lysozyme that HyHEL-5 exhibits relative to D44.1.
-
-	Three-dimensional structures of the free and the antigen-complexed Fab from monoclonal anti-lysozyme antibody D44.1.,Braden BC, Souchon H, Eisele JL, Bentley GA, Bhat TN, Navaza J, Poljak RJ J Mol Biol. 1994 Nov 4;243(4):767-81. PMID:7966295<ref>PMID:7966295</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1mlb" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]		*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Bentley~~, G A~~]]	+	[[Category: Bentley GA]]
-	[[Category: Bhat~~, T N~~]]	+	[[Category: Bhat TN]]
-	[[Category: Braden~~, B C~~]]	+	[[Category: Braden BC]]
-	[[Category: Eisele, J L]]	+	[[Category: Eisele J-L]]
-	[[Category: Navaza, J]]	+	[[Category: Navaza J]]
-	[[Category: Poljak~~, R J~~]]	+	[[Category: Poljak RJ]]
-	[[Category: Souchon, H]]	+	[[Category: Souchon H]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin~~]]	+

OCA at 09:27, 5 May 2021

OCA — Wed, 05 May 2021 09:27:50 GMT

←Older revision		Revision as of 09:27, 5 May 2021
Line 3:		Line 3:
	<StructureSection load='1mlb' size='340' side='right'caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1mlb' size='340' side='right'caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MLB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [~~http~~://pdbe.org/1mlb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mlb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlb ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [https://pdbe.org/1mlb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [https://www.ebi.ac.uk/pdbsum/1mlb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlb ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:02, 31 July 2019

OCA — Wed, 31 Jul 2019 08:02:47 GMT

←Older revision		Revision as of 08:02, 31 July 2019
Line 1:		Line 1:

	==MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME==		==MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME==
-	<StructureSection load='1mlb' size='340' side='right' caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1mlb' size='340' side='right'caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLB FirstGlance]. <br>
Line 10:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ml/1mlb_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ml/1mlb_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:

	==See Also==		==See Also==
-	*[[3D structures ~~of monoclonal antibody~~\|3D structures ~~of monoclonal antibody~~]]	+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Bentley, G A]]		[[Category: Bentley, G A]]

OCA at 10:23, 25 October 2017

OCA — Wed, 25 Oct 2017 10:23:49 GMT

←Older revision		Revision as of 10:23, 25 October 2017
Line 1:		Line 1:
		+
	==MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME==		==MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME==
	<StructureSection load='1mlb' size='340' side='right' caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1mlb' size='340' side='right' caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [http://pdbe.org/1mlb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [http://www.ebi.ac.uk/pdbsum/1mlb PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [http://pdbe.org/1mlb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [http://www.ebi.ac.uk/pdbsum/1mlb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlb ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mlb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:

	==See Also==		==See Also==
-	*[[~~Monoclonal Antibody~~\|~~Monoclonal Antibody~~]]	+	*[[3D structures of monoclonal antibody\|3D structures of monoclonal antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:31, 11 September 2015

OCA — Fri, 11 Sep 2015 23:31:10 GMT

←Older revision		Revision as of 23:31, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1mlb' size='340' side='right' caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1mlb' size='340' side='right' caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [http://www.ebi.ac.uk/pdbsum/1mlb PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [http://pdbe.org/1mlb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [http://www.ebi.ac.uk/pdbsum/1mlb PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mlb" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Bentley, G A]]		[[Category: Bentley, G A]]
	[[Category: Bhat, T N]]		[[Category: Bhat, T N]]

OCA at 17:10, 5 January 2015

OCA — Mon, 05 Jan 2015 17:10:56 GMT

←Older revision		Revision as of 17:10, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLB FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [http://www.ebi.ac.uk/pdbsum/1mlb PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [http://www.ebi.ac.uk/pdbsum/1mlb PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Bentley, G A.]]	+	[[Category: Bentley, G A]]
-	[[Category: Bhat, T N.]]	+	[[Category: Bhat, T N]]
-	[[Category: Braden, B C.]]	+	[[Category: Braden, B C]]
-	[[Category: Eisele, J L.]]	+	[[Category: Eisele, J L]]
-	[[Category: Navaza, J.]]	+	[[Category: Navaza, J]]
-	[[Category: Poljak, R J.]]	+	[[Category: Poljak, R J]]
-	[[Category: Souchon, H.]]	+	[[Category: Souchon, H]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]

OCA at 04:29, 3 October 2014

OCA — Fri, 03 Oct 2014 04:29:47 GMT

←Older revision		Revision as of 04:29, 3 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1mlb\| PDB=1mlb \| SCENE= }}~~	+	==MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME==
-	===MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME===	+	<StructureSection load='1mlb' size='340' side='right' caption='[[1mlb]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_7966295}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLB FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mlb RCSB], [http://www.ebi.ac.uk/pdbsum/1mlb PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ml/1mlb_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The three-dimensional structures of the free and antigen-complexed Fabs from the mouse monoclonal anti-hen egg white lysozyme antibody D44.1 have been solved and refined by X-ray crystallographic techniques. The crystals of the free and lysozyme-bound Fabs were grown under identical conditions and their X-ray diffraction data were collected to 2.1 and 2.5 A, respectively. Two molecules of the Fab-lysozyme complex in the asymmetric unit of the crystals show nearly identical conformations and thus confirm the essential structural features of the antigen-antibody interface. Three buried water molecules enhance the surface complementarity at the interface and provide hydrogen bonds to stabilize the complex. Two hydrophobic buried holes are present at the interface which, although large enough to accommodate solvent molecules, are void. The combining site residues of the complexed FabD44.1 exhibit reduced temperature factors compared with those of the free Fab. Furthermore, small perturbations in atomic positions and rearrangements of side-chains at the combining site, and a relative rearrangement of the variable domains of the light (VL) and the heavy (VH) chains, detail a Fab accommodation of the bound lysozyme. The amino acid sequence of the VH domain, as well as the epitope of lysozyme recognized by D44.1 are very close to those previously reported for the monoclonal antibody HyHEL-5. A feature central to the FabD44.1 and FabHyHEL-5 complexes with lysozyme are three salt bridges between VH glutamate residues 35 and 50 and lysozyme arginine residues 45 and 68. The presence of the three salt bridges in the D44.1-lysozyme interface indicates that these bonds are not responsible for the 1000-fold increase in affinity for lysozyme that HyHEL-5 exhibits relative to D44.1.

-	~~==About this Structure==~~	+	Three-dimensional structures of the free and the antigen-complexed Fab from monoclonal anti-lysozyme antibody D44.1.,Braden BC, Souchon H, Eisele JL, Bentley GA, Bhat TN, Navaza J, Poljak RJ J Mol Biol. 1994 Nov 4;243(4):767-81. PMID:7966295<ref>PMID:7966295</ref>
-	~~[[1mlb]] is a 2 chain structure with sequence~~ from ~~[http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1MLB OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Monoclonal Antibody\|Monoclonal Antibody]]		*[[Monoclonal Antibody\|Monoclonal Antibody]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:007966295</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Bentley, G A.]]		[[Category: Bentley, G A.]]

OCA at 03:02, 20 September 2013

OCA — Fri, 20 Sep 2013 03:02:53 GMT

←Older revision		Revision as of 03:02, 20 September 2013
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-	[[Image:1mlb.png\|left\|200px]]
-
	{{STRUCTURE_1mlb\| PDB=1mlb \| SCENE= }}		{{STRUCTURE_1mlb\| PDB=1mlb \| SCENE= }}
-
	===MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME===		===MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME===
-
	{{ABSTRACT_PUBMED_7966295}}		{{ABSTRACT_PUBMED_7966295}}

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	==Reference==		==Reference==
-	<ref group="xtra">PMID:007966295</ref><references group="xtra"/>	+	<ref group="xtra">PMID:007966295</ref><references group="xtra"/><references/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Bentley, G A.]]		[[Category: Bentley, G A.]]
Line 23:		Line 19:
	[[Category: Poljak, R J.]]		[[Category: Poljak, R J.]]
	[[Category: Souchon, H.]]		[[Category: Souchon, H.]]
		+	[[Category: Immune system]]
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]

OCA at 21:29, 20 October 2012

OCA — Sat, 20 Oct 2012 21:29:18 GMT

←Older revision		Revision as of 21:29, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1mlb]] is a 2 chain structure ~~of [[Monoclonal Antibody]]~~ with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA].	+	[[1mlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLB OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:007966295~~</ref><ref group="xtra">PMID:009250664~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:007966295</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Bentley, G A.]]		[[Category: Bentley, G A.]]

OCA: Protected "1mlb" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 13:24:14 GMT

Protected "1mlb" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:24, 27 July 2012