1mlx - Revision history

OCA at 07:45, 14 February 2024

OCA — Wed, 14 Feb 2024 07:45:30 GMT

←Older revision		Revision as of 07:45, 14 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.25Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [https://pdbe.org/1mlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [https://www.ebi.ac.uk/pdbsum/1mlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [https://pdbe.org/1mlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [https://www.ebi.ac.uk/pdbsum/1mlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlx ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A novel 2'-modification, 2'-O-[2-(methylthio)ethyl] or 2'-O-MTE, has been incorporated into oligonucleotides and evaluated for properties relevant to antisense activity. The results were compared with the previously characterized 2'-O-[2-(methoxy)ethyl] 2'-O-MOE modification. As expected, the 2'-O-MTE modified oligonucleotides exhibited improved binding to human serum albumin compared to the 2'-O-MOE modified oligonucleotides. The 2'-O-MTE oligonucleotides maintained high binding affinity to target RNA. Nuclease digestion of 2'-O-MTE oligonucleotides showed that they have limited resistance to exonuclease degradation. We analyzed the crystal structure of a decamer DNA duplex containing the 2'-O-MTE modifcation. Analysis of the crystal structure provides insight into the improved RNA binding affinity, protein binding affinity and limited resistance of 2'-O-MTE modified oligonucleotides to exonuclease degradation.
-
-	2'-O-[2-(methylthio)ethyl]-modified oligonucleotide: an analogue of 2'-O-[2-(methoxy)-ethyl]-modified oligonucleotide with improved protein binding properties and high binding affinity to target RNA.,Prakash TP, Manoharan M, Kawasaki AM, Fraser AS, Lesnik EA, Sioufi N, Leeds JM, Teplova M, Egli M Biochemistry. 2002 Oct 1;41(39):11642-8. PMID:12269806<ref>PMID:12269806</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1mlx" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:09, 25 January 2023

OCA — Wed, 25 Jan 2023 07:09:01 GMT

←Older revision		Revision as of 07:09, 25 January 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [https://pdbe.org/1mlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [https://www.ebi.ac.uk/pdbsum/1mlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [https://pdbe.org/1mlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [https://www.ebi.ac.uk/pdbsum/1mlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlx ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Egli, M]]	+	[[Category: Egli M]]
-	[[Category: Fraser~~, A S~~]]	+	[[Category: Fraser AS]]
-	[[Category: Kawasaki~~, A M~~]]	+	[[Category: Kawasaki AM]]
-	[[Category: Leeds~~, J M~~]]	+	[[Category: Leeds JM]]
-	[[Category: Lesnik~~, E A~~]]	+	[[Category: Lesnik EA]]
-	[[Category: Manoharan, M]]	+	[[Category: Manoharan M]]
-	[[Category: Prakash~~, T P~~]]	+	[[Category: Prakash TP]]
-	[[Category: Sioufi, N]]	+	[[Category: Sioufi N]]
-	[[Category: Teplova, M]]	+	[[Category: Teplova M]]
-	~~[[Category: A-form]]~~	+
-	~~[[Category: Antisense modification]]~~	+
-	~~[[Category: Dna]]~~	+
-	~~[[Category: Double helix]]~~	+
-	~~[[Category: High resolution structure]]~~	+
-	~~[[Category: Hydration]]~~	+
-	~~[[Category: Nuclease resistance]]~~	+
-	~~[[Category: Nucleic acid analogue]]~~	+
-	~~[[Category: Protein binding affinity]]~~	+
-	~~[[Category: Rna affinity~~]]	+

OCA at 08:54, 21 April 2021

OCA — Wed, 21 Apr 2021 08:54:38 GMT

←Older revision		Revision as of 08:54, 21 April 2021
Line 3:		Line 3:
	<StructureSection load='1mlx' size='340' side='right'caption='[[1mlx]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='1mlx' size='340' side='right'caption='[[1mlx]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MLX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [~~http~~://pdbe.org/1mlx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mlx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [https://pdbe.org/1mlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [https://www.ebi.ac.uk/pdbsum/1mlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:24, 17 July 2019

OCA — Wed, 17 Jul 2019 13:24:50 GMT

←Older revision		Revision as of 13:24, 17 July 2019
Line 1:		Line 1:

	==Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex==		==Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex==
-	<StructureSection load='1mlx' size='340' side='right' caption='[[1mlx]], [[Resolution\|resolution]] 1.25Å' scene=''>	+	<StructureSection load='1mlx' size='340' side='right'caption='[[1mlx]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Egli, M]]		[[Category: Egli, M]]
	[[Category: Fraser, A S]]		[[Category: Fraser, A S]]

OCA at 09:11, 12 October 2017

OCA — Thu, 12 Oct 2017 09:11:57 GMT

←Older revision		Revision as of 09:11, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex==		==Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex==
	<StructureSection load='1mlx' size='340' side='right' caption='[[1mlx]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='1mlx' size='340' side='right' caption='[[1mlx]], [[Resolution\|resolution]] 1.25Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [http://pdbe.org/1mlx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [http://www.ebi.ac.uk/pdbsum/1mlx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [http://pdbe.org/1mlx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [http://www.ebi.ac.uk/pdbsum/1mlx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:49, 11 September 2015

OCA — Fri, 11 Sep 2015 10:49:44 GMT

←Older revision		Revision as of 10:49, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [http://www.ebi.ac.uk/pdbsum/1mlx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [http://pdbe.org/1mlx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [http://www.ebi.ac.uk/pdbsum/1mlx PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mlx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:38, 22 December 2014

OCA — Mon, 22 Dec 2014 05:38:45 GMT

←Older revision		Revision as of 05:38, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [http://www.ebi.ac.uk/pdbsum/1mlx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [http://www.ebi.ac.uk/pdbsum/1mlx PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Egli, M.]]	+	[[Category: Egli, M]]
-	[[Category: Fraser, A S.]]	+	[[Category: Fraser, A S]]
-	[[Category: Kawasaki, A M.]]	+	[[Category: Kawasaki, A M]]
-	[[Category: Leeds, J M.]]	+	[[Category: Leeds, J M]]
-	[[Category: Lesnik, E A.]]	+	[[Category: Lesnik, E A]]
-	[[Category: Manoharan, M.]]	+	[[Category: Manoharan, M]]
-	[[Category: Prakash, T P.]]	+	[[Category: Prakash, T P]]
-	[[Category: Sioufi, N.]]	+	[[Category: Sioufi, N]]
-	[[Category: Teplova, M.]]	+	[[Category: Teplova, M]]
	[[Category: A-form]]		[[Category: A-form]]
	[[Category: Antisense modification]]		[[Category: Antisense modification]]

OCA at 05:34, 8 June 2014

OCA — Sun, 08 Jun 2014 05:34:03 GMT

←Older revision		Revision as of 05:34, 8 June 2014
Line 1:		Line 1:
-	[[~~Image:~~1mlx.~~png~~\|~~left\|200px~~]]	+	==Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex==
		+	<StructureSection load='1mlx' size='340' side='right' caption='[[1mlx]], [[Resolution\|resolution]] 1.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [http://www.ebi.ac.uk/pdbsum/1mlx PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A novel 2'-modification, 2'-O-[2-(methylthio)ethyl] or 2'-O-MTE, has been incorporated into oligonucleotides and evaluated for properties relevant to antisense activity. The results were compared with the previously characterized 2'-O-[2-(methoxy)ethyl] 2'-O-MOE modification. As expected, the 2'-O-MTE modified oligonucleotides exhibited improved binding to human serum albumin compared to the 2'-O-MOE modified oligonucleotides. The 2'-O-MTE oligonucleotides maintained high binding affinity to target RNA. Nuclease digestion of 2'-O-MTE oligonucleotides showed that they have limited resistance to exonuclease degradation. We analyzed the crystal structure of a decamer DNA duplex containing the 2'-O-MTE modifcation. Analysis of the crystal structure provides insight into the improved RNA binding affinity, protein binding affinity and limited resistance of 2'-O-MTE modified oligonucleotides to exonuclease degradation.

-	~~{{STRUCTURE_1mlx\| PDB=1mlx \| SCENE= }}~~	+	2'-O-[2-(methylthio)ethyl]-modified oligonucleotide: an analogue of 2'-O-[2-(methoxy)-ethyl]-modified oligonucleotide with improved protein binding properties and high binding affinity to target RNA.,Prakash TP, Manoharan M, Kawasaki AM, Fraser AS, Lesnik EA, Sioufi N, Leeds JM, Teplova M, Egli M Biochemistry. 2002 Oct 1;41(39):11642-8. PMID:12269806<ref>PMID:12269806</ref>

-	~~===Crystal Structure Analysis~~ of ~~a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12269806}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1mlx]] is a 2 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=1MLX OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012269806<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Egli, M.]]		[[Category: Egli, M.]]
	[[Category: Fraser, A S.]]		[[Category: Fraser, A S.]]

OCA: Protected "1mlx" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 15:58:28 GMT

Protected "1mlx" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:58, 14 August 2012

OCA at 15:58, 14 August 2012

OCA — Tue, 14 Aug 2012 15:58:27 GMT

←Older revision		Revision as of 15:58, 14 August 2012
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-	{{Seed}}
	[[Image:1mlx.png\|left\|200px]]		[[Image:1mlx.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1mlx\| PDB=1mlx \| SCENE= }}		{{STRUCTURE_1mlx\| PDB=1mlx \| SCENE= }}

	===Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex===		===Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12269806}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_12269806}}		{{ABSTRACT_PUBMED_12269806}}

	==About this Structure==		==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA].	+	[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA].

	==Reference==		==Reference==
-	2'-O-[2-(methylthio)ethyl]-modified oligonucleotide: an analogue of 2'-O-[2-(methoxy)-ethyl]-modified oligonucleotide with improved protein binding properties and high binding affinity to target RNA., Prakash TP, Manoharan M, Kawasaki AM, Fraser AS, Lesnik EA, Sioufi N, Leeds JM, Teplova M, Egli M, Biochemistry. 2002 Oct 1;41(39):11642-8. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~12269806 12269806]~~	+	<ref group="xtra">PMID:012269806</ref><references group="xtra"/>
	[[Category: Egli, M.]]		[[Category: Egli, M.]]
	[[Category: Fraser, A S.]]		[[Category: Fraser, A S.]]
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	[[Category: Protein binding affinity]]		[[Category: Protein binding affinity]]
	[[Category: Rna affinity]]		[[Category: Rna affinity]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:17:52 2008''