1mnt - Revision history

OCA at 08:50, 22 May 2024

OCA — Wed, 22 May 2024 08:50:32 GMT

←Older revision		Revision as of 08:50, 22 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mnt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mnt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Wild-type Mnt repressor of Salmonella bacteriophage P22 is a tetrameric protein of 82 residues per monomer. A C-terminal deletion mutant of the repressor denoted Mnt (1-76) is a dimer in solution. The structure of this dimer has been determined using NMR. The NMR assignments of the majority of the 1H, 15N, and 13C resonances were obtained using 2D and triple-resonance 3D techniques. Elements of secondary structure were identified on the basis of characteristic sequential and medium range NOEs. For the structure determination more than 1000 NOEs per monomer were obtained, and structures were generated using distance geometry and restrained simulated annealing calculations. The discrimination of intra- vs intermonomer NOEs was based upon the observation of intersubunit NOEs in [15N,13C] double half-filtered NOESY experiments. The N-terminal part of Mnt (residues 1-44), which shows a 40% sequence homology with the Arc repressor, has a similar secondary and tertiary structure. Mnt (1-76) continues with a loop region of irregular structure, a third alpha-helix, and a random coil C-terminal peptide. Analysis of the secondary structure NOEs, the exchange rates, and the backbone chemical shifts suggests that the carboxy-terminal third helix is less stable than the remainder of the protein, but the observation of intersubunit NOEs for this part of the protein enables the positioning of this helix. The rsmd's between the backbone atoms of the N-terminal part of the Mnt repressor (residues 5-43, 5'-43') and the Arc repressor is 1.58 A, and between this region and the corresponding part of the MetJ repressor 1.43 A.
		+
		+	Solution structure of dimeric Mnt repressor (1-76).,Burgering MJ, Boelens R, Gilbert DE, Breg JN, Knight KL, Sauer RT, Kaptein R Biochemistry. 1994 Dec 20;33(50):15036-45. PMID:7999761<ref>PMID:7999761</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1mnt" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:38, 10 April 2024

OCA — Wed, 10 Apr 2024 08:38:35 GMT

←Older revision		Revision as of 08:38, 10 April 2024
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)==		==SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)==
-	<StructureSection load='1mnt' size='340' side='right'caption='[[1mnt~~]], [[NMR_Ensembles_of_Models \| 15 NMR models~~]]' scene=''>	+	<StructureSection load='1mnt' size='340' side='right'caption='[[1mnt]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bpp22 Bpp22~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MNT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MNT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [https://pdbe.org/1mnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [https://www.ebi.ac.uk/pdbsum/1mnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mnt ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [https://pdbe.org/1mnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [https://www.ebi.ac.uk/pdbsum/1mnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mnt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RMNT_BPP22 RMNT_BPP22]] Mnt acts as a transcriptional repressor of genes ant and arc.	+	[https://www.uniprot.org/uniprot/RMNT_BPP22 RMNT_BPP22] Mnt acts as a transcriptional repressor of genes ant and arc.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mnt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mnt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Wild-type Mnt repressor of Salmonella bacteriophage P22 is a tetrameric protein of 82 residues per monomer. A C-terminal deletion mutant of the repressor denoted Mnt (1-76) is a dimer in solution. The structure of this dimer has been determined using NMR. The NMR assignments of the majority of the 1H, 15N, and 13C resonances were obtained using 2D and triple-resonance 3D techniques. Elements of secondary structure were identified on the basis of characteristic sequential and medium range NOEs. For the structure determination more than 1000 NOEs per monomer were obtained, and structures were generated using distance geometry and restrained simulated annealing calculations. The discrimination of intra- vs intermonomer NOEs was based upon the observation of intersubunit NOEs in [15N,13C] double half-filtered NOESY experiments. The N-terminal part of Mnt (residues 1-44), which shows a 40% sequence homology with the Arc repressor, has a similar secondary and tertiary structure. Mnt (1-76) continues with a loop region of irregular structure, a third alpha-helix, and a random coil C-terminal peptide. Analysis of the secondary structure NOEs, the exchange rates, and the backbone chemical shifts suggests that the carboxy-terminal third helix is less stable than the remainder of the protein, but the observation of intersubunit NOEs for this part of the protein enables the positioning of this helix. The rsmd's between the backbone atoms of the N-terminal part of the Mnt repressor (residues 5-43, 5'-43') and the Arc repressor is 1.58 A, and between this region and the corresponding part of the MetJ repressor 1.43 A.
-
-	Solution structure of dimeric Mnt repressor (1-76).,Burgering MJ, Boelens R, Gilbert DE, Breg JN, Knight KL, Sauer RT, Kaptein R Biochemistry. 1994 Dec 20;33(50):15036-45. PMID:7999761<ref>PMID:7999761</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1mnt" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bpp22]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boelens, R]]	+	[[Category: Salmonella virus P22]]
-	[[Category: Breg~~, J N~~]]	+	[[Category: Boelens R]]
-	[[Category: Burgering~~, M J.M~~]]	+	[[Category: Breg JN]]
-	[[Category: Gilbert~~, D E~~]]	+	[[Category: Burgering MJM]]
-	[[Category: Kaptein, R]]	+	[[Category: Gilbert DE]]
-	[[Category: Knight~~, K L~~]]	+	[[Category: Kaptein R]]
-	[[Category: Sauer~~, R T]]~~	+	[[Category: Knight KL]]
-	~~[[Category: Transcription regulation~~]]	+	[[Category: Sauer RT]]

OCA at 06:53, 25 August 2021

OCA — Wed, 25 Aug 2021 06:53:49 GMT

←Older revision		Revision as of 06:53, 25 August 2021
Line 3:		Line 3:
	<StructureSection load='1mnt' size='340' side='right'caption='[[1mnt]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>		<StructureSection load='1mnt' size='340' side='right'caption='[[1mnt]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MNT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MNT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [~~http~~://pdbe.org/1mnt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mnt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mnt ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [https://pdbe.org/1mnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [https://www.ebi.ac.uk/pdbsum/1mnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mnt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RMNT_BPP22 RMNT_BPP22]] Mnt acts as a transcriptional repressor of genes ant and arc.	+	[[https://www.uniprot.org/uniprot/RMNT_BPP22 RMNT_BPP22]] Mnt acts as a transcriptional repressor of genes ant and arc.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:21, 20 November 2019

OCA — Wed, 20 Nov 2019 18:21:45 GMT

←Older revision		Revision as of 18:21, 20 November 2019
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)==		==SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)==
-	<StructureSection load='1mnt' size='340' side='right' caption='[[1mnt]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>	+	<StructureSection load='1mnt' size='340' side='right'caption='[[1mnt]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MNT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MNT FirstGlance]. <br>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Bpp22]]		[[Category: Bpp22]]
		+	[[Category: Large Structures]]
	[[Category: Boelens, R]]		[[Category: Boelens, R]]
	[[Category: Breg, J N]]		[[Category: Breg, J N]]

OCA at 07:48, 31 January 2018

OCA — Wed, 31 Jan 2018 07:48:56 GMT

←Older revision		Revision as of 07:48, 31 January 2018
Line 1:		Line 1:
		+
	==SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)==		==SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)==
	<StructureSection load='1mnt' size='340' side='right' caption='[[1mnt]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>		<StructureSection load='1mnt' size='340' side='right' caption='[[1mnt]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MNT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MNT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [http://pdbe.org/1mnt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [http://www.ebi.ac.uk/pdbsum/1mnt PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [http://pdbe.org/1mnt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [http://www.ebi.ac.uk/pdbsum/1mnt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mnt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mn/1mnt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mn/1mnt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 02:34, 9 February 2016

OCA — Tue, 09 Feb 2016 02:34:15 GMT

←Older revision		Revision as of 02:34, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mnt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:45, 10 September 2015

OCA — Thu, 10 Sep 2015 17:45:49 GMT

←Older revision		Revision as of 17:45, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1mnt' size='340' side='right' caption='[[1mnt]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>		<StructureSection load='1mnt' size='340' side='right' caption='[[1mnt]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_p22 Enterobacteria phage p22~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MNT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MNT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [http://www.ebi.ac.uk/pdbsum/1mnt PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [http://pdbe.org/1mnt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [http://www.ebi.ac.uk/pdbsum/1mnt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mnt" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage p22~~]]	+	[[Category: Bpp22]]
	[[Category: Boelens, R]]		[[Category: Boelens, R]]
	[[Category: Breg, J N]]		[[Category: Breg, J N]]

OCA at 03:48, 25 December 2014

OCA — Thu, 25 Dec 2014 03:48:49 GMT

←Older revision		Revision as of 03:48, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MNT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MNT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [http://www.ebi.ac.uk/pdbsum/1mnt PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [http://www.ebi.ac.uk/pdbsum/1mnt PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RMNT_BPP22 RMNT_BPP22]] Mnt acts as a transcriptional repressor of genes ant and arc.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage p22]]		[[Category: Enterobacteria phage p22]]
-	[[Category: Boelens, R.]]	+	[[Category: Boelens, R]]
-	[[Category: Breg, J N.]]	+	[[Category: Breg, J N]]
-	[[Category: Burgering, M J.M.]]	+	[[Category: Burgering, M J.M]]
-	[[Category: Gilbert, D E.]]	+	[[Category: Gilbert, D E]]
-	[[Category: Kaptein, R.]]	+	[[Category: Kaptein, R]]
-	[[Category: Knight, K L.]]	+	[[Category: Knight, K L]]
-	[[Category: Sauer, R T.]]	+	[[Category: Sauer, R T]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

OCA at 14:23, 28 September 2014

OCA — Sun, 28 Sep 2014 14:23:03 GMT

←Older revision		Revision as of 14:23, 28 September 2014
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-	[[~~Image~~:1mnt.~~png\|left~~\|200px]]	+	==SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)==
		+	<StructureSection load='1mnt' size='340' side='right' caption='[[1mnt]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mnt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MNT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mnt RCSB], [http://www.ebi.ac.uk/pdbsum/1mnt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mn/1mnt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Wild-type Mnt repressor of Salmonella bacteriophage P22 is a tetrameric protein of 82 residues per monomer. A C-terminal deletion mutant of the repressor denoted Mnt (1-76) is a dimer in solution. The structure of this dimer has been determined using NMR. The NMR assignments of the majority of the 1H, 15N, and 13C resonances were obtained using 2D and triple-resonance 3D techniques. Elements of secondary structure were identified on the basis of characteristic sequential and medium range NOEs. For the structure determination more than 1000 NOEs per monomer were obtained, and structures were generated using distance geometry and restrained simulated annealing calculations. The discrimination of intra- vs intermonomer NOEs was based upon the observation of intersubunit NOEs in [15N,13C] double half-filtered NOESY experiments. The N-terminal part of Mnt (residues 1-44), which shows a 40% sequence homology with the Arc repressor, has a similar secondary and tertiary structure. Mnt (1-76) continues with a loop region of irregular structure, a third alpha-helix, and a random coil C-terminal peptide. Analysis of the secondary structure NOEs, the exchange rates, and the backbone chemical shifts suggests that the carboxy-terminal third helix is less stable than the remainder of the protein, but the observation of intersubunit NOEs for this part of the protein enables the positioning of this helix. The rsmd's between the backbone atoms of the N-terminal part of the Mnt repressor (residues 5-43, 5'-43') and the Arc repressor is 1.58 A, and between this region and the corresponding part of the MetJ repressor 1.43 A.

-	~~{{STRUCTURE_1mnt\| PDB=1mnt \| SCENE= }}~~	+	Solution structure of dimeric Mnt repressor (1-76).,Burgering MJ, Boelens R, Gilbert DE, Breg JN, Knight KL, Sauer RT, Kaptein R Biochemistry. 1994 Dec 20;33(50):15036-45. PMID:7999761<ref>PMID:7999761</ref>

-	~~===SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7999761}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1mnt]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:007999761<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterobacteria phage p22]]		[[Category: Enterobacteria phage p22]]
	[[Category: Boelens, R.]]		[[Category: Boelens, R.]]

OCA: Protected "1mnt" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 19:49:19 GMT

Protected "1mnt" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:49, 5 December 2012