1mwo - Revision history

OCA at 08:42, 10 April 2024

OCA — Wed, 10 Apr 2024 08:42:52 GMT

←Older revision		Revision as of 08:42, 10 April 2024
Line 3:		Line 3:
	<StructureSection load='1mwo' size='340' side='right'caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1mwo' size='340' side='right'caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"pyrococcus_furiosus_subsp._woesei"_kanoksilapatham_et_al._2004 "pyrococcus furiosus subsp.~~ woesei~~" kanoksilapatham et al. 2004~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1mxd\|1mxd]], [[1mxg\|1mxg]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [https://pdbe.org/1mwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [https://www.ebi.ac.uk/pdbsum/1mwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [https://pdbe.org/1mwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [https://www.ebi.ac.uk/pdbsum/1mwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q7LYT7_PYRWO Q7LYT7_PYRWO]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mwo ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mwo ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of the alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei was solved in the presence of three inhibitors: acarbose, Tris, and zinc. In the absence of exogenous metals, this alpha-amylase bound 1 and 4 molar eq of zinc and calcium, respectively. The structure reveals a novel, activating, two-metal (Ca,Zn)-binding site and a second inhibitory zinc-binding site that is found in the -1 sugar-binding pocket within the active site. The data resolve the apparent paradox between the zinc requirement for catalytic activity and its strong inhibitory effect when added in molar excess. They provide a rationale as to why this alpha-amylase, in contrast to commercially available alpha-amylases, does not require the addition of metal ions for full catalytic activity, suggesting it as an ideal target to maximize the efficiency of industrial processes like liquefaction of starch.
-
-	Differential regulation of a hyperthermophilic alpha-amylase with a novel (Ca,Zn) two-metal center by zinc.,Linden A, Mayans O, Meyer-Klaucke W, Antranikian G, Wilmanns M J Biol Chem. 2003 Mar 14;278(11):9875-84. Epub 2002 Dec 12. PMID:12482867<ref>PMID:12482867</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1mwo" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Amylase 3D structures\|Amylase 3D structures]]		*[[Amylase 3D structures\|Amylase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pyrococcus furiosus subsp. woesei kanoksilapatham et al. 2004]]
-	[[Category: Alpha-amylase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Antranikian, G]]	+	[[Category: Pyrococcus woesei]]
-	[[Category: Linden, A]]	+	[[Category: Antranikian G]]
-	[[Category: Mayans, O]]	+	[[Category: Linden A]]
-	[[Category: Meyer-Klaucke, W]]	+	[[Category: Mayans O]]
-	[[Category: Wilmanns, M]]	+	[[Category: Meyer-Klaucke W]]
-	~~[[Category: Hydrolase~~]]	+	[[Category: Wilmanns M]]

OCA at 06:56, 25 August 2021

OCA — Wed, 25 Aug 2021 06:56:33 GMT

←Older revision		Revision as of 06:56, 25 August 2021
Line 3:		Line 3:
	<StructureSection load='1mwo' size='340' side='right'caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1mwo' size='340' side='right'caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"pyrococcus_furiosus_subsp._woesei"_kanoksilapatham_et_al._2004 "pyrococcus furiosus subsp. woesei" kanoksilapatham et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MWO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"pyrococcus_furiosus_subsp._woesei"_kanoksilapatham_et_al._2004 "pyrococcus furiosus subsp. woesei" kanoksilapatham et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mxd\|1mxd]], [[1mxg\|1mxg]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1mxd\|1mxd]], [[1mxg\|1mxg]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [~~http~~://pdbe.org/1mwo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mwo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [https://pdbe.org/1mwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [https://www.ebi.ac.uk/pdbsum/1mwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwo ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 18:23, 20 November 2019

OCA — Wed, 20 Nov 2019 18:23:57 GMT

←Older revision		Revision as of 18:23, 20 November 2019
Line 1:		Line 1:

	==Crystal Structure Analysis of the Hyperthermostable Pyrocoocus woesei alpha-amylase==		==Crystal Structure Analysis of the Hyperthermostable Pyrocoocus woesei alpha-amylase==
-	<StructureSection load='1mwo' size='340' side='right' caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1mwo' size='340' side='right'caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"pyrococcus_furiosus_subsp._woesei"_kanoksilapatham_et_al._2004 "pyrococcus furiosus subsp. woesei" kanoksilapatham et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"pyrococcus_furiosus_subsp._woesei"_kanoksilapatham_et_al._2004 "pyrococcus furiosus subsp. woesei" kanoksilapatham et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWO FirstGlance]. <br>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1mwo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1mwo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Amylase 3D structures\|Amylase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	[[Category: Pyrococcus furiosus subsp. woesei kanoksilapatham et al. 2004]]		[[Category: Pyrococcus furiosus subsp. woesei kanoksilapatham et al. 2004]]
	[[Category: Alpha-amylase]]		[[Category: Alpha-amylase]]
		+	[[Category: Large Structures]]
	[[Category: Antranikian, G]]		[[Category: Antranikian, G]]
	[[Category: Linden, A]]		[[Category: Linden, A]]

OCA at 07:50, 31 January 2018

OCA — Wed, 31 Jan 2018 07:50:51 GMT

←Older revision		Revision as of 07:50, 31 January 2018
Line 1:		Line 1:
		+
	==Crystal Structure Analysis of the Hyperthermostable Pyrocoocus woesei alpha-amylase==		==Crystal Structure Analysis of the Hyperthermostable Pyrocoocus woesei alpha-amylase==
	<StructureSection load='1mwo' size='340' side='right' caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1mwo' size='340' side='right' caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mxd\|1mxd]], [[1mxg\|1mxg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mxd\|1mxd]], [[1mxg\|1mxg]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [http://pdbe.org/1mwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [http://www.ebi.ac.uk/pdbsum/1mwo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [http://pdbe.org/1mwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [http://www.ebi.ac.uk/pdbsum/1mwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwo ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mwo_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mwo_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1mwo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1mwo" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Amylase\|Amylase]]
-	*[[User:Gabriel Pons/Sandbox 2\|User:Gabriel Pons/Sandbox 2]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:03, 9 February 2016

OCA — Tue, 09 Feb 2016 13:03:58 GMT

←Older revision		Revision as of 13:03, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mwo ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:32, 10 September 2015

OCA — Thu, 10 Sep 2015 22:32:06 GMT

←Older revision		Revision as of 22:32, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1mwo' size='340' side='right' caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1mwo' size='340' side='right' caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrococcus_woesei Pyrococcus~~ woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"pyrococcus_furiosus_subsp._woesei"_kanoksilapatham_et_al._2004 "pyrococcus furiosus subsp. woesei" kanoksilapatham et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mxd\|1mxd]], [[1mxg\|1mxg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mxd\|1mxd]], [[1mxg\|1mxg]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [http://www.ebi.ac.uk/pdbsum/1mwo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [http://pdbe.org/1mwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [http://www.ebi.ac.uk/pdbsum/1mwo PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mwo" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pyrococcus furiosus subsp. woesei kanoksilapatham et al. 2004]]
	[[Category: Alpha-amylase]]		[[Category: Alpha-amylase]]
-	[[Category: Pyrococcus woesei]]
	[[Category: Antranikian, G]]		[[Category: Antranikian, G]]
	[[Category: Linden, A]]		[[Category: Linden, A]]

OCA at 15:36, 5 January 2015

OCA — Mon, 05 Jan 2015 15:36:04 GMT

←Older revision		Revision as of 15:36, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWO FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mxd\|1mxd]], [[1mxg\|1mxg]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mxd\|1mxd]], [[1mxg\|1mxg]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [http://www.ebi.ac.uk/pdbsum/1mwo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [http://www.ebi.ac.uk/pdbsum/1mwo PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	==See Also==		==See Also==
	*[[Amylase\|Amylase]]		*[[Amylase\|Amylase]]
		+	*[[User:Gabriel Pons/Sandbox 2\|User:Gabriel Pons/Sandbox 2]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 36:
	[[Category: Alpha-amylase]]		[[Category: Alpha-amylase]]
	[[Category: Pyrococcus woesei]]		[[Category: Pyrococcus woesei]]
-	[[Category: Antranikian, G.]]	+	[[Category: Antranikian, G]]
-	[[Category: Linden, A.]]	+	[[Category: Linden, A]]
-	[[Category: Mayans, O.]]	+	[[Category: Mayans, O]]
-	[[Category: Meyer-Klaucke, W.]]	+	[[Category: Meyer-Klaucke, W]]
-	[[Category: Wilmanns, M~~.]]~~	+	[[Category: Wilmanns, M]]
-	~~[[Category: Alpha-amylase~~]]	+
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 13:13, 28 September 2014

OCA — Sun, 28 Sep 2014 13:13:18 GMT

←Older revision		Revision as of 13:13, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1mwo.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure Analysis of the Hyperthermostable Pyrocoocus woesei alpha-amylase==
		+	<StructureSection load='1mwo' size='340' side='right' caption='[[1mwo]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mwo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWO FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mxd\|1mxd]], [[1mxg\|1mxg]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mwo RCSB], [http://www.ebi.ac.uk/pdbsum/1mwo PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mwo_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei was solved in the presence of three inhibitors: acarbose, Tris, and zinc. In the absence of exogenous metals, this alpha-amylase bound 1 and 4 molar eq of zinc and calcium, respectively. The structure reveals a novel, activating, two-metal (Ca,Zn)-binding site and a second inhibitory zinc-binding site that is found in the -1 sugar-binding pocket within the active site. The data resolve the apparent paradox between the zinc requirement for catalytic activity and its strong inhibitory effect when added in molar excess. They provide a rationale as to why this alpha-amylase, in contrast to commercially available alpha-amylases, does not require the addition of metal ions for full catalytic activity, suggesting it as an ideal target to maximize the efficiency of industrial processes like liquefaction of starch.

-	~~{{STRUCTURE_1mwo\| PDB=1mwo \| SCENE= }}~~	+	Differential regulation of a hyperthermophilic alpha-amylase with a novel (Ca,Zn) two-metal center by zinc.,Linden A, Mayans O, Meyer-Klaucke W, Antranikian G, Wilmanns M J Biol Chem. 2003 Mar 14;278(11):9875-84. Epub 2002 Dec 12. PMID:12482867<ref>PMID:12482867</ref>

-	~~===Crystal Structure Analysis~~ of the ~~Hyperthermostable Pyrocoocus woesei alpha-amylase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12482867}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1mwo]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pyrococcus_woesei Pyrococcus woesei]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA].~~	+

	==See Also==		==See Also==
-	*[[~~Alpha-~~Amylase\|~~Alpha-~~Amylase]]	+	*[[Amylase\|Amylase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012482867<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Alpha-amylase]]		[[Category: Alpha-amylase]]
	[[Category: Pyrococcus woesei]]		[[Category: Pyrococcus woesei]]

OCA at 13:56, 20 October 2012

OCA — Sat, 20 Oct 2012 13:56:18 GMT

←Older revision		Revision as of 13:56, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1mwo]] is a 1 chain structure ~~of [[Alpha-Amylase]]~~ with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA].	+	[[1mwo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWO OCA].

	==See Also==		==See Also==

OCA: Protected "1mwo" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 13:34:01 GMT

Protected "1mwo" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:34, 26 July 2012