1mws - Revision history

OCA at 08:42, 10 April 2024

OCA — Wed, 10 Apr 2024 08:42:58 GMT

←Older revision		Revision as of 08:42, 10 April 2024
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	<StructureSection load='1mws' size='340' side='right'caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1mws' size='340' side='right'caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>~~, ~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~NC1~~:~~NITROCEFIN~~+~~ACYL-SERINE~~'>~~NC1~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1mwr\|1mwr]], [[1mwt\|1mwt]], [[1mwu\|1mwu]], [[1mwx\|1mwx]]~~</~~div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [https://pdbe.org/1mws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [https://www.ebi.ac.uk/pdbsum/1mws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mws ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [https://pdbe.org/1mws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [https://www.ebi.ac.uk/pdbsum/1mws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mws ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q93IC2_STAAU Q93IC2_STAAU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mws ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mws ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The multiple antibiotic resistance of methicillin-resistant strains of Staphylococcus aureus (MRSA) has become a major clinical problem worldwide. The key determinant of the broad-spectrum beta-lactam resistance in MRSA strains is the penicillin-binding protein 2a (PBP2a). Because of its low affinity for beta-lactams, PBP2a provides transpeptidase activity to allow cell wall synthesis at beta-lactam concentrations that inhibit the beta-lactam-sensitive PBPs normally produced by S. aureus. The crystal structure of a soluble derivative of PBP2a has been determined to 1.8 A resolution and provides the highest resolution structure for a high molecular mass PBP. Additionally, structures of the acyl-PBP complexes of PBP2a with nitrocefin, penicillin G and methicillin allow, for the first time, a comparison of an apo and acylated resistant PBP. An analysis of the PBP2a active site in these forms reveals the structural basis of its resistance and identifies features in newly developed beta-lactams that are likely important for high affinity binding.
-
-	Structural basis for the beta lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus.,Lim D, Strynadka NC Nat Struct Biol. 2002 Nov;9(11):870-6. PMID:12389036<ref>PMID:12389036</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1mws" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Penicillin-binding protein 3D structures\|Penicillin-binding protein 3D structures]]		*[[Penicillin-binding protein 3D structures\|Penicillin-binding protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lim, D C~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Strynadka, N C.J~~]]	+	[[Category: Lim DC]]
-	[[Category: ~~Beta-lactam]]~~	+	[[Category: Strynadka NCJ]]
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: D-carboxypeptidase]]~~	+
-	~~[[Category: D-transpeptidase]]~~	+
-	~~[[Category: Nitrocefin]]~~	+
-	~~[[Category: Penicillin binding protein~~]]	+

OCA at 06:56, 25 August 2021

OCA — Wed, 25 Aug 2021 06:56:38 GMT

←Older revision		Revision as of 06:56, 25 August 2021
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	<StructureSection load='1mws' size='340' side='right'caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1mws' size='340' side='right'caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mwr\|1mwr]], [[1mwt\|1mwt]], [[1mwu\|1mwu]], [[1mwx\|1mwx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1mwr\|1mwr]], [[1mwt\|1mwt]], [[1mwu\|1mwu]], [[1mwx\|1mwx]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [~~http~~://pdbe.org/1mws PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mws PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mws ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [https://pdbe.org/1mws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [https://www.ebi.ac.uk/pdbsum/1mws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mws ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 18:44, 20 November 2019

OCA — Wed, 20 Nov 2019 18:44:03 GMT

←Older revision		Revision as of 18:44, 20 November 2019
Line 1:		Line 1:

	==Structure of nitrocefin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.00 A resolution.==		==Structure of nitrocefin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.00 A resolution.==
-	<StructureSection load='1mws' size='340' side='right' caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1mws' size='340' side='right'caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWS FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWS FirstGlance]. <br>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1mws" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1mws" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Penicillin-binding protein 3D structures\|Penicillin-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lim, D C]]		[[Category: Lim, D C]]
	[[Category: Strynadka, N C.J]]		[[Category: Strynadka, N C.J]]

OCA at 08:08, 31 January 2018

OCA — Wed, 31 Jan 2018 08:08:02 GMT

←Older revision		Revision as of 08:08, 31 January 2018
Line 1:		Line 1:
		+
	==Structure of nitrocefin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.00 A resolution.==		==Structure of nitrocefin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.00 A resolution.==
	<StructureSection load='1mws' size='340' side='right' caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1mws' size='340' side='right' caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mwr\|1mwr]], [[1mwt\|1mwt]], [[1mwu\|1mwu]], [[1mwx\|1mwx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mwr\|1mwr]], [[1mwt\|1mwt]], [[1mwu\|1mwu]], [[1mwx\|1mwx]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [http://pdbe.org/1mws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [http://www.ebi.ac.uk/pdbsum/1mws PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [http://pdbe.org/1mws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [http://www.ebi.ac.uk/pdbsum/1mws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mws ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mws_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mws_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1mws" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1mws" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Penicillin-binding protein\|Penicillin-binding protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:43, 9 February 2016

OCA — Tue, 09 Feb 2016 06:43:00 GMT

←Older revision		Revision as of 06:43, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mws ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:37, 10 September 2015

OCA — Thu, 10 Sep 2015 19:37:13 GMT

←Older revision		Revision as of 19:37, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1mws' size='340' side='right' caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1mws' size='340' side='right' caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mwr\|1mwr]], [[1mwt\|1mwt]], [[1mwu\|1mwu]], [[1mwx\|1mwx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mwr\|1mwr]], [[1mwt\|1mwt]], [[1mwu\|1mwu]], [[1mwx\|1mwx]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [http://www.ebi.ac.uk/pdbsum/1mws PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [http://pdbe.org/1mws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [http://www.ebi.ac.uk/pdbsum/1mws PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mws" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Staphylococcus aureus]]
	[[Category: Lim, D C]]		[[Category: Lim, D C]]
	[[Category: Strynadka, N C.J]]		[[Category: Strynadka, N C.J]]

OCA at 16:04, 5 January 2015

OCA — Mon, 05 Jan 2015 16:04:50 GMT

←Older revision		Revision as of 16:04, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWS FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mwr\|1mwr]], [[1mwt\|1mwt]], [[1mwu\|1mwu]], [[1mwx\|1mwx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mwr\|1mwr]], [[1mwt\|1mwt]], [[1mwu\|1mwu]], [[1mwx\|1mwx]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [http://www.ebi.ac.uk/pdbsum/1mws PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [http://www.ebi.ac.uk/pdbsum/1mws PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Lim, D C.]]	+	[[Category: Lim, D C]]
-	[[Category: Strynadka, N C.J.]]	+	[[Category: Strynadka, N C.J]]
	[[Category: Beta-lactam]]		[[Category: Beta-lactam]]
	[[Category: Biosynthetic protein]]		[[Category: Biosynthetic protein]]

OCA at 10:30, 3 October 2014

OCA — Fri, 03 Oct 2014 10:30:22 GMT

←Older revision		Revision as of 10:30, 3 October 2014
Line 1:		Line 1:
-	[[~~Image:~~1mws.~~png~~\|~~left~~\|200px]]	+	==Structure of nitrocefin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.00 A resolution.==
		+	<StructureSection load='1mws' size='340' side='right' caption='[[1mws]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1mwr\|1mwr]], [[1mwt\|1mwt]], [[1mwu\|1mwu]], [[1mwx\|1mwx]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [http://www.ebi.ac.uk/pdbsum/1mws PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mws_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The multiple antibiotic resistance of methicillin-resistant strains of Staphylococcus aureus (MRSA) has become a major clinical problem worldwide. The key determinant of the broad-spectrum beta-lactam resistance in MRSA strains is the penicillin-binding protein 2a (PBP2a). Because of its low affinity for beta-lactams, PBP2a provides transpeptidase activity to allow cell wall synthesis at beta-lactam concentrations that inhibit the beta-lactam-sensitive PBPs normally produced by S. aureus. The crystal structure of a soluble derivative of PBP2a has been determined to 1.8 A resolution and provides the highest resolution structure for a high molecular mass PBP. Additionally, structures of the acyl-PBP complexes of PBP2a with nitrocefin, penicillin G and methicillin allow, for the first time, a comparison of an apo and acylated resistant PBP. An analysis of the PBP2a active site in these forms reveals the structural basis of its resistance and identifies features in newly developed beta-lactams that are likely important for high affinity binding.

-	~~{{STRUCTURE_1mws\| PDB=1mws \| SCENE= }}~~	+	Structural basis for the beta lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus.,Lim D, Strynadka NC Nat Struct Biol. 2002 Nov;9(11):870-6. PMID:12389036<ref>PMID:12389036</ref>

-	~~===Structure~~ of ~~nitrocefin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2~~.~~00 A resolution~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12389036}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1mws]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA].~~	+

	==See Also==		==See Also==
	*[[Penicillin-binding protein\|Penicillin-binding protein]]		*[[Penicillin-binding protein\|Penicillin-binding protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012389036<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Lim, D C.]]		[[Category: Lim, D C.]]

OCA at 13:00, 20 October 2012

OCA — Sat, 20 Oct 2012 13:00:14 GMT

←Older revision		Revision as of 13:00, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1mws]] is a 2 chain structure ~~of [[Penicillin-binding protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA].	+	[[1mws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA].

	==See Also==		==See Also==

OCA: Protected "1mws" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 10:35:36 GMT

Protected "1mws" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:35, 26 July 2012