1mzt - Revision history

OCA at 18:51, 29 November 2023

OCA — Wed, 29 Nov 2023 18:51:58 GMT

←Older revision		Revision as of 18:51, 29 November 2023
Line 1:		Line 1:

	==NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes==		==NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes==
-	<StructureSection load='1mzt' size='340' side='right'caption='[[1mzt~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1mzt' size='340' side='right'caption='[[1mzt]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MZT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MZT FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1fdm\|1fdm]]</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [https://pdbe.org/1mzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [https://www.ebi.ac.uk/pdbsum/1mzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mzt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [https://pdbe.org/1mzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [https://www.ebi.ac.uk/pdbsum/1mzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mzt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CAPSD_BPFD CAPSD_BPFD]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).	+	[https://www.uniprot.org/uniprot/CAPSD_BPFD CAPSD_BPFD] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	[[Category: Enterobacteria phage fd]]		[[Category: Enterobacteria phage fd]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Marassi~~, F M~~]]	+	[[Category: Marassi FM]]
-	[[Category: Opella~~, S J]]~~	+	[[Category: Opella SJ]]
-	~~[[Category: Fd coat protein]]~~	+
-	~~[[Category: Membrane-bound]]~~	+
-	~~[[Category: Pviii]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 09:31, 5 May 2021

OCA — Wed, 05 May 2021 09:31:17 GMT

←Older revision		Revision as of 09:31, 5 May 2021
Line 3:		Line 3:
	<StructureSection load='1mzt' size='340' side='right'caption='[[1mzt]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1mzt' size='340' side='right'caption='[[1mzt]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MZT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MZT FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fdm\|1fdm]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fdm\|1fdm]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [~~http~~://pdbe.org/1mzt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mzt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mzt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [https://pdbe.org/1mzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [https://www.ebi.ac.uk/pdbsum/1mzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mzt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CAPSD_BPFD CAPSD_BPFD]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).	+	[[https://www.uniprot.org/uniprot/CAPSD_BPFD CAPSD_BPFD]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:20, 31 July 2019

OCA — Wed, 31 Jul 2019 07:20:21 GMT

←Older revision		Revision as of 07:20, 31 July 2019
Line 1:		Line 1:

	==NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes==		==NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes==
-	<StructureSection load='1mzt' size='340' side='right' caption='[[1mzt]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1mzt' size='340' side='right'caption='[[1mzt]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage fd]]		[[Category: Enterobacteria phage fd]]
		+	[[Category: Large Structures]]
	[[Category: Marassi, F M]]		[[Category: Marassi, F M]]
	[[Category: Opella, S J]]		[[Category: Opella, S J]]

OCA at 10:10, 25 October 2017

OCA — Wed, 25 Oct 2017 10:10:05 GMT

←Older revision		Revision as of 10:10, 25 October 2017
Line 1:		Line 1:
		+
	==NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes==		==NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes==
	<StructureSection load='1mzt' size='340' side='right' caption='[[1mzt]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1mzt' size='340' side='right' caption='[[1mzt]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bpfd Bpfd~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fdm\|1fdm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fdm\|1fdm]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [http://pdbe.org/1mzt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [http://www.ebi.ac.uk/pdbsum/1mzt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [http://pdbe.org/1mzt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [http://www.ebi.ac.uk/pdbsum/1mzt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mzt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bpfd~~]]	+	[[Category: Enterobacteria phage fd]]
	[[Category: Marassi, F M]]		[[Category: Marassi, F M]]
	[[Category: Opella, S J]]		[[Category: Opella, S J]]

OCA at 06:42, 11 September 2015

OCA — Fri, 11 Sep 2015 06:42:23 GMT

←Older revision		Revision as of 06:42, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1mzt' size='340' side='right' caption='[[1mzt]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1mzt' size='340' side='right' caption='[[1mzt]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_fd Enterobacteria phage fd~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpfd Bpfd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fdm\|1fdm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fdm\|1fdm]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [http://www.ebi.ac.uk/pdbsum/1mzt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [http://pdbe.org/1mzt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [http://www.ebi.ac.uk/pdbsum/1mzt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~COATB_BPFD COATB_BPFD~~]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).	+	[[http://www.uniprot.org/uniprot/CAPSD_BPFD CAPSD_BPFD]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mzt" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage fd~~]]	+	[[Category: Bpfd]]
	[[Category: Marassi, F M]]		[[Category: Marassi, F M]]
	[[Category: Opella, S J]]		[[Category: Opella, S J]]

OCA at 12:44, 25 December 2014

OCA — Thu, 25 Dec 2014 12:44:13 GMT

←Older revision		Revision as of 12:44, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fdm\|1fdm]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fdm\|1fdm]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [http://www.ebi.ac.uk/pdbsum/1mzt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [http://www.ebi.ac.uk/pdbsum/1mzt PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/COATB_BPFD COATB_BPFD]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage fd]]		[[Category: Enterobacteria phage fd]]
-	[[Category: Marassi, F M.]]	+	[[Category: Marassi, F M]]
-	[[Category: Opella, S J.]]	+	[[Category: Opella, S J]]
	[[Category: Fd coat protein]]		[[Category: Fd coat protein]]
	[[Category: Membrane-bound]]		[[Category: Membrane-bound]]
	[[Category: Pviii]]		[[Category: Pviii]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 14:51, 28 September 2014

OCA — Sun, 28 Sep 2014 14:51:47 GMT

←Older revision		Revision as of 14:51, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1mzt.~~png~~\|~~left~~\|~~200px~~]]	+	==NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes==
		+	<StructureSection load='1mzt' size='340' side='right' caption='[[1mzt]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fdm\|1fdm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [http://www.ebi.ac.uk/pdbsum/1mzt PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A solid-state NMR approach for simultaneous resonance assignment and three-dimensional structure determination of a membrane protein in lipid bilayers is described. The approach is based on the scattering, hence the descriptor "shotgun," of (15)N-labeled amino acids throughout the protein sequence (and the resulting NMR spectra). The samples are obtained by protein expression in bacteria grown on media in which one type of amino acid is labeled and the others are not. Shotgun NMR short-circuits the laborious and time-consuming process of obtaining complete sequential assignments prior to the calculation of a protein structure from the NMR data by taking advantage of the orientational information inherent to the spectra of aligned proteins. As a result, it is possible to simultaneously assign resonances and measure orientational restraints for structure determination. A total of five two-dimensional (1)H/(15)N PISEMA (polarization inversion spin exchange at the magic angle) spectra, from one uniformly and four selectively (15)N-labeled samples, were sufficient to determine the structure of the membrane-bound form of the 50-residue major pVIII coat protein of fd filamentous bacteriophage. Pisa (polarity index slat angle) wheels are an essential element in the process, which starts with the simultaneous assignment of resonances and the assembly of isolated polypeptide segments, and culminates in the complete three-dimensional structure of the protein with atomic resolution. The principles are also applicable to weakly aligned proteins studied by solution NMR spectroscopy. [The structure we determined for the membrane-bound form of the Fd bacteriophage pVIII coat protein has been deposited in the Protein Data Bank as PDB file 1MZT.]

-	~~{{STRUCTURE_1mzt\| PDB=1mzt \| SCENE= }}~~	+	Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints.,Marassi FM, Opella SJ Protein Sci. 2003 Mar;12(3):403-11. PMID:12592011<ref>PMID:12592011</ref>

-	~~===NMR structure~~ of the ~~fd bacteriophage pVIII coat protein in lipid bilayer membranes===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12592011}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1mzt]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA].~~	+	</StructureSection>
	[[Category: Enterobacteria phage fd]]		[[Category: Enterobacteria phage fd]]
	[[Category: Marassi, F M.]]		[[Category: Marassi, F M.]]

OCA: Protected "1mzt" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 20:38:58 GMT

Protected "1mzt" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:38, 5 December 2012

OCA at 20:38, 5 December 2012

OCA — Wed, 05 Dec 2012 20:38:55 GMT

←Older revision		Revision as of 20:38, 5 December 2012
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-	{{Seed}}
	[[Image:1mzt.png\|left\|200px]]		[[Image:1mzt.png\|left\|200px]]

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	{{STRUCTURE_1mzt\| PDB=1mzt \| SCENE= }}		{{STRUCTURE_1mzt\| PDB=1mzt \| SCENE= }}

	===NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes===		===NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes===

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	{{ABSTRACT_PUBMED_12592011}}		{{ABSTRACT_PUBMED_12592011}}

	==About this Structure==		==About this Structure==
-	~~1MZT is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/~~Filamentous_phage Filamentous~~ phage]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA].	+	[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA].
-		+	[[Category: Enterobacteria phage fd]]
-	~~==Reference==~~	+
-	Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints., Marassi FM, Opella SJ, Protein Sci. 2003 Mar;12(3):403-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12592011 12592011]	+
-	[[Category: ~~Filamentous~~ phage]]	+
-	~~[[Category: Single protein~~]]	+
	[[Category: Marassi, F M.]]		[[Category: Marassi, F M.]]
	[[Category: Opella, S J.]]		[[Category: Opella, S J.]]
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	[[Category: Membrane-bound]]		[[Category: Membrane-bound]]
	[[Category: Pviii]]		[[Category: Pviii]]
-		+	[[Category: Viral protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Jul 28 23:11:29 2008''~~	+

OCA at 20:11, 28 July 2008

OCA — Mon, 28 Jul 2008 20:11:36 GMT

←Older revision		Revision as of 20:11, 28 July 2008
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	{{STRUCTURE_1mzt\| PDB=1mzt \| SCENE= }}		{{STRUCTURE_1mzt\| PDB=1mzt \| SCENE= }}

-	~~'''~~NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes~~'''~~	+	===NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes===


-	~~==Overview==~~	+	<!--
-	~~A solid~~-~~state NMR approach for simultaneous resonance assignment and three~~-~~dimensional structure determination of a membrane protein in lipid bilayers is described.~~ The ~~approach is based on the scattering~~, ~~hence the descriptor "shotgun~~,~~" of (15)N-labeled amino acids throughout~~ the protein sequence (and the resulting NMR spectra). The samples are obtained by protein expression in bacteria grown on media in which one type of amino acid is labeled and the others are not. Shotgun NMR short-circuits the laborious and time-consuming process of obtaining complete sequential assignments prior to the ~~calculation of a protein structure from the NMR data by taking advantage of the orientational information inherent to the spectra of aligned proteins. As a result,~~ it ~~is possible to simultaneously assign resonances and measure orientational restraints for structure determination. A total of five two-dimensional (1)H~~/(15)N PISEMA (polarization inversion spin exchange at the magic angle) spectra, from one uniformly and four selectively (15)N-labeled samples, were sufficient to determine the structure of the membrane-bound form of the 50-residue major pVIII coat protein of fd filamentous bacteriophage. ~~Pisa (polarity index slat angle~~) ~~wheels are an essential element in the process~~, ~~which starts with~~ the ~~simultaneous assignment of resonances and the assembly of isolated polypeptide segments, and culminates in the complete three-dimensional structure of the protein with atomic resolution~~. ~~The principles are also applicable to weakly aligned proteins studied by solution NMR spectroscopy. [The structure we determined for the membrane~~-~~bound form of the Fd bacteriophage pVIII coat protein has been deposited in the Protein Data Bank as PDB file 1MZT.]~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_12592011}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 12592011 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_12592011}}

	==About this Structure==		==About this Structure==
-	1MZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA].	+	1MZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA].

	==Reference==		==Reference==
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	[[Category: Membrane-bound]]		[[Category: Membrane-bound]]
	[[Category: Pviii]]		[[Category: Pviii]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 01~~:54:45 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:11:29 2008''