1n02 - Revision history

OCA at 08:44, 10 April 2024

OCA — Wed, 10 Apr 2024 08:44:05 GMT

←Older revision		Revision as of 08:44, 10 April 2024
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	==Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N==		==Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N==
-	<StructureSection load='1n02' size='340' side='right'caption='[[1n02~~]], [[NMR_Ensembles_of_Models \| 26 NMR models~~]]' scene=''>	+	<StructureSection load='1n02' size='340' side='right'caption='[[1n02]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Anabaena_variabilis_var._ellipsospora Anabaena variabilis var. ellipsospora~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N02 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N02 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[2ezm\|2ezm]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [https://pdbe.org/1n02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [https://www.ebi.ac.uk/pdbsum/1n02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n02 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [https://pdbe.org/1n02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [https://www.ebi.ac.uk/pdbsum/1n02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n02 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CVN_NOSEL CVN_NOSEL]] Mannose-binding lectin.<ref>PMID:9210678</ref> <ref>PMID:12678493</ref>	+	[https://www.uniprot.org/uniprot/CVN_NOSEL CVN_NOSEL] Mannose-binding lectin.<ref>PMID:9210678</ref> <ref>PMID:12678493</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n02 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n02 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The high-resolution solution structure of a monomeric circular permuted (cp) variant of the potent HIV-inactivating protein cyanovirin-N (CV-N) was determined by NMR. Comparison with the wild-type (wt) structure revealed that the observed loss in stability of cpCV-N compared to the wt protein is due to less favorable packing of several residues at the pseudo twofold axis that are responsible for holding the two halves of the molecule together. In particular, the N and C-terminal amino acid residues exhibit conformational flexibility, resulting in fewer and less favorable contacts between them. The important hydrophobic and hydrogen-bonding network between residues W49, D89, H90, Y100 and E101 that was observed in wt CV-N is no longer present. For instance, Y100 and E101 are flexible and the tryptophan side-chain is in a different conformation compared to the wt protein. The stability loss amounts to approximately 2kcal/mol and the mobility of the protein is evident by fast amide proton exchange throughout the chain. Mutation of the single proline residue to glycine (P52G) did not substantially affect the stability of the protein, in contrast to the finding for wtCV-N. The binding of high-mannose type oligosaccharides to cpCV-N was also investigated. Similar to wtCV-N, two carbohydrate-binding sites were identified on the protein and the Man alpha1-->2Man linked moieties on the sugar were delineated as binding epitopes. Unlike in wtCV-N, the binding sites on cpCV-N are structurally similar and exhibit comparable binding affinities for the respective sugars. On the basis of the studies presented here and previous results on high-mannose binding to wtCV-N, we discuss a model for the interaction between gp120 and CV-N.
-
-	Solution structure of a circular-permuted variant of the potent HIV-inactivating protein cyanovirin-N: structural basis for protein stability and oligosaccharide interaction.,Barrientos LG, Louis JM, Ratner DM, Seeberger PH, Gronenborn AM J Mol Biol. 2003 Jan 3;325(1):211-23. PMID:12473463<ref>PMID:12473463</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1n02" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Anabaena variabilis var. ellipsospora]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Barrientos, L G~~]]	+	[[Category: Nostoc ellipsosporum]]
-	[[Category: ~~Gronenborn, A M~~]]	+	[[Category: Barrientos LG]]
-	[[Category: ~~Viral protein inhibitor]]~~	+	[[Category: Gronenborn AM]]
-	~~[[Category: Virus/viral protein inhibitor~~]]	+

OCA at 08:14, 10 November 2021

OCA — Wed, 10 Nov 2021 08:14:46 GMT

←Older revision		Revision as of 08:14, 10 November 2021
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	<StructureSection load='1n02' size='340' side='right'caption='[[1n02]], [[NMR_Ensembles_of_Models \| 26 NMR models]]' scene=''>		<StructureSection load='1n02' size='340' side='right'caption='[[1n02]], [[NMR_Ensembles_of_Models \| 26 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Anabaena_variabilis_var._ellipsospora Anabaena variabilis var. ellipsospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1N02 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena_variabilis_var._ellipsospora Anabaena variabilis var. ellipsospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N02 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ezm\|2ezm]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ezm\|2ezm]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [~~http~~://pdbe.org/1n02 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1n02 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1n02 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [https://pdbe.org/1n02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [https://www.ebi.ac.uk/pdbsum/1n02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n02 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CVN_NOSEL CVN_NOSEL]] Mannose-binding lectin.<ref>PMID:9210678</ref> <ref>PMID:12678493</ref>	+	[[https://www.uniprot.org/uniprot/CVN_NOSEL CVN_NOSEL]] Mannose-binding lectin.<ref>PMID:9210678</ref> <ref>PMID:12678493</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:17, 31 July 2019

OCA — Wed, 31 Jul 2019 07:17:36 GMT

←Older revision		Revision as of 07:17, 31 July 2019
Line 1:		Line 1:

	==Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N==		==Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N==
-	<StructureSection load='1n02' size='340' side='right' caption='[[1n02]], [[NMR_Ensembles_of_Models \| 26 NMR models]]' scene=''>	+	<StructureSection load='1n02' size='340' side='right'caption='[[1n02]], [[NMR_Ensembles_of_Models \| 26 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis_var._ellipsospora Anabaena variabilis var. ellipsospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N02 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis_var._ellipsospora Anabaena variabilis var. ellipsospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N02 FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/1n02_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/1n02_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Anabaena variabilis var. ellipsospora]]		[[Category: Anabaena variabilis var. ellipsospora]]
		+	[[Category: Large Structures]]
	[[Category: Barrientos, L G]]		[[Category: Barrientos, L G]]
	[[Category: Gronenborn, A M]]		[[Category: Gronenborn, A M]]
	[[Category: Viral protein inhibitor]]		[[Category: Viral protein inhibitor]]
	[[Category: Virus/viral protein inhibitor]]		[[Category: Virus/viral protein inhibitor]]

OCA at 10:07, 25 October 2017

OCA — Wed, 25 Oct 2017 10:07:44 GMT

←Older revision		Revision as of 10:07, 25 October 2017
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		+
	==Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N==		==Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N==
	<StructureSection load='1n02' size='340' side='right' caption='[[1n02]], [[NMR_Ensembles_of_Models \| 26 NMR models]]' scene=''>		<StructureSection load='1n02' size='340' side='right' caption='[[1n02]], [[NMR_Ensembles_of_Models \| 26 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis_var._ellipsospora Anabaena variabilis var. ellipsospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N02 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis_var._ellipsospora Anabaena variabilis var. ellipsospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N02 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ezm\|2ezm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ezm\|2ezm]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [http://pdbe.org/1n02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [http://www.ebi.ac.uk/pdbsum/1n02 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [http://pdbe.org/1n02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [http://www.ebi.ac.uk/pdbsum/1n02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n02 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n02 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:37, 11 September 2015

OCA — Fri, 11 Sep 2015 23:37:29 GMT

←Older revision		Revision as of 23:37, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1n02' size='340' side='right' caption='[[1n02]], [[NMR_Ensembles_of_Models \| 26 NMR models]]' scene=''>		<StructureSection load='1n02' size='340' side='right' caption='[[1n02]], [[NMR_Ensembles_of_Models \| 26 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Nostoc_ellipsosporum Nostoc ellipsosporum~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N02 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis_var._ellipsospora Anabaena variabilis var. ellipsospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N02 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ezm\|2ezm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ezm\|2ezm]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [http://www.ebi.ac.uk/pdbsum/1n02 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [http://pdbe.org/1n02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [http://www.ebi.ac.uk/pdbsum/1n02 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1n02" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Nostoc ellipsosporum~~]]	+	[[Category: Anabaena variabilis var. ellipsospora]]
	[[Category: Barrientos, L G]]		[[Category: Barrientos, L G]]
	[[Category: Gronenborn, A M]]		[[Category: Gronenborn, A M]]
	[[Category: Viral protein inhibitor]]		[[Category: Viral protein inhibitor]]
	[[Category: Virus/viral protein inhibitor]]		[[Category: Virus/viral protein inhibitor]]

OCA at 20:55, 25 December 2014

OCA — Thu, 25 Dec 2014 20:55:16 GMT

←Older revision		Revision as of 20:55, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N02 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N02 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ezm\|2ezm]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ezm\|2ezm]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [http://www.ebi.ac.uk/pdbsum/1n02 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [http://www.ebi.ac.uk/pdbsum/1n02 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CVN_NOSEL CVN_NOSEL]] Mannose-binding lectin.<ref>PMID:9210678</ref> <ref>PMID:12678493</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Nostoc ellipsosporum]]		[[Category: Nostoc ellipsosporum]]
-	[[Category: Barrientos, L G.]]	+	[[Category: Barrientos, L G]]
-	[[Category: Gronenborn, A M.]]	+	[[Category: Gronenborn, A M]]
	[[Category: Viral protein inhibitor]]		[[Category: Viral protein inhibitor]]
	[[Category: Virus/viral protein inhibitor]]		[[Category: Virus/viral protein inhibitor]]

OCA at 15:23, 28 September 2014

OCA — Sun, 28 Sep 2014 15:23:33 GMT

←Older revision		Revision as of 15:23, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1n02.~~png~~\|~~left~~\|200px]]	+	==Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N==
		+	<StructureSection load='1n02' size='340' side='right' caption='[[1n02]], [[NMR_Ensembles_of_Models \| 26 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1n02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N02 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ezm\|2ezm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n02 RCSB], [http://www.ebi.ac.uk/pdbsum/1n02 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/1n02_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The high-resolution solution structure of a monomeric circular permuted (cp) variant of the potent HIV-inactivating protein cyanovirin-N (CV-N) was determined by NMR. Comparison with the wild-type (wt) structure revealed that the observed loss in stability of cpCV-N compared to the wt protein is due to less favorable packing of several residues at the pseudo twofold axis that are responsible for holding the two halves of the molecule together. In particular, the N and C-terminal amino acid residues exhibit conformational flexibility, resulting in fewer and less favorable contacts between them. The important hydrophobic and hydrogen-bonding network between residues W49, D89, H90, Y100 and E101 that was observed in wt CV-N is no longer present. For instance, Y100 and E101 are flexible and the tryptophan side-chain is in a different conformation compared to the wt protein. The stability loss amounts to approximately 2kcal/mol and the mobility of the protein is evident by fast amide proton exchange throughout the chain. Mutation of the single proline residue to glycine (P52G) did not substantially affect the stability of the protein, in contrast to the finding for wtCV-N. The binding of high-mannose type oligosaccharides to cpCV-N was also investigated. Similar to wtCV-N, two carbohydrate-binding sites were identified on the protein and the Man alpha1-->2Man linked moieties on the sugar were delineated as binding epitopes. Unlike in wtCV-N, the binding sites on cpCV-N are structurally similar and exhibit comparable binding affinities for the respective sugars. On the basis of the studies presented here and previous results on high-mannose binding to wtCV-N, we discuss a model for the interaction between gp120 and CV-N.

-	~~{{STRUCTURE_1n02\| PDB=1n02 \| SCENE= }}~~	+	Solution structure of a circular-permuted variant of the potent HIV-inactivating protein cyanovirin-N: structural basis for protein stability and oligosaccharide interaction.,Barrientos LG, Louis JM, Ratner DM, Seeberger PH, Gronenborn AM J Mol Biol. 2003 Jan 3;325(1):211-23. PMID:12473463<ref>PMID:12473463</ref>

-	~~===Solution Structure of~~ a ~~Circular-Permuted Variant~~ of the ~~Potent HIV-inactivating Protein Cyanovirin-N===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12473463}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1n02]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012473463<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Nostoc ellipsosporum]]		[[Category: Nostoc ellipsosporum]]
	[[Category: Barrientos, L G.]]		[[Category: Barrientos, L G.]]

OCA: Protected "1n02" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 19:35:41 GMT

Protected "1n02" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:35, 5 December 2012

OCA at 19:35, 5 December 2012

OCA — Wed, 05 Dec 2012 19:35:38 GMT

←Older revision		Revision as of 19:35, 5 December 2012
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-	{{Seed}}
	[[Image:1n02.png\|left\|200px]]		[[Image:1n02.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1n02", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1n02\| PDB=1n02 \| SCENE= }}		{{STRUCTURE_1n02\| PDB=1n02 \| SCENE= }}

	===Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N===		===Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12473463}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12473463 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12473463}}		{{ABSTRACT_PUBMED_12473463}}

	==About this Structure==		==About this Structure==
-	~~1N02~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA].	+	[[1n02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12473463~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012473463</ref><references group="xtra"/>
	[[Category: Nostoc ellipsosporum]]		[[Category: Nostoc ellipsosporum]]
	[[Category: Barrientos, L G.]]		[[Category: Barrientos, L G.]]
	[[Category: Gronenborn, A M.]]		[[Category: Gronenborn, A M.]]
		+	[[Category: Viral protein inhibitor]]
	[[Category: Virus/viral protein inhibitor]]		[[Category: Virus/viral protein inhibitor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:56:47 2009''

OCA at 04:56, 18 February 2009

OCA — Wed, 18 Feb 2009 04:56:48 GMT

←Older revision		Revision as of 04:56, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1N02 is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA].	+	1N02 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA].

	==Reference==		==Reference==
-	Solution structure of a circular-permuted variant of the potent HIV-inactivating protein cyanovirin-N: structural basis for protein stability and oligosaccharide interaction., Barrientos LG, Louis JM, Ratner DM, Seeberger PH, Gronenborn AM, J Mol Biol. 2003 Jan 3;325(1):211-23. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/12473463 12473463]~~	+	<ref group="xtra">PMID:12473463</ref><references group="xtra"/>
	[[Category: Nostoc ellipsosporum]]		[[Category: Nostoc ellipsosporum]]
-	[[Category: Single protein]]
	[[Category: Barrientos, L G.]]		[[Category: Barrientos, L G.]]
	[[Category: Gronenborn, A M.]]		[[Category: Gronenborn, A M.]]
	[[Category: Virus/viral protein inhibitor]]		[[Category: Virus/viral protein inhibitor]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 13~~:43:~~50 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:56:47 2009''