1n3j - Revision history

OCA at 08:52, 22 May 2024

OCA — Wed, 22 May 2024 08:52:23 GMT

←Older revision		Revision as of 08:52, 22 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3j ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Site-specific lysine methylation of histones by SET domains is a hallmark for epigenetic control of gene transcription in eukaryotic organisms. Here we report that a SET domain protein from Paramecium bursaria chlorella virus can specifically di-methylate Lys27 in histone H3, a modification implicated in gene silencing. The solution structure of the viral SET domain reveals a butterfly-shaped head-to-head symmetric dimer different from other known protein methyltransferases. Each subunit consists of a Greek-key antiparallel beta-barrel and a three-stranded open-faced sandwich that mediates the dimer interface. Cofactor S-adenosyl-L-methionine (SAM) binds at the opening of the beta-barrel, and amino acids C-terminal to Lys27 in H3 and in the flexible C-terminal tail of the enzyme confer the specificity of this viral histone methyltransferase.
		+
		+	A dimeric viral SET domain methyltransferase specific to Lys27 of histone H3.,Manzur KL, Farooq A, Zeng L, Plotnikova O, Koch AW, Sachchidanand, Zhou MM Nat Struct Biol. 2003 Mar;10(3):187-96. PMID:12567185<ref>PMID:12567185</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1n3j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Histone methyltransferase 3D structures\|Histone methyltransferase 3D structures]]		*[[Histone methyltransferase 3D structures\|Histone methyltransferase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:45, 10 April 2024

OCA — Wed, 10 Apr 2024 08:45:21 GMT

←Older revision		Revision as of 08:45, 10 April 2024
Line 1:		Line 1:

	==Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1==		==Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1==
-	<StructureSection load='1n3j' size='340' side='right'caption='[[1n3j~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1n3j' size='340' side='right'caption='[[1n3j]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chlorella_pbcv-1_virus~~ Chlorella ~~pbcv-~~1 ~~virus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N3J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N3J FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>A612L ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10506 Chlorella PBCV-1 virus])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [https://pdbe.org/1n3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [https://www.ebi.ac.uk/pdbsum/1n3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [https://pdbe.org/1n3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [https://www.ebi.ac.uk/pdbsum/1n3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O41094_PBCV1 O41094_PBCV1]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3j ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Site-specific lysine methylation of histones by SET domains is a hallmark for epigenetic control of gene transcription in eukaryotic organisms. Here we report that a SET domain protein from Paramecium bursaria chlorella virus can specifically di-methylate Lys27 in histone H3, a modification implicated in gene silencing. The solution structure of the viral SET domain reveals a butterfly-shaped head-to-head symmetric dimer different from other known protein methyltransferases. Each subunit consists of a Greek-key antiparallel beta-barrel and a three-stranded open-faced sandwich that mediates the dimer interface. Cofactor S-adenosyl-L-methionine (SAM) binds at the opening of the beta-barrel, and amino acids C-terminal to Lys27 in H3 and in the flexible C-terminal tail of the enzyme confer the specificity of this viral histone methyltransferase.
-
-	A dimeric viral SET domain methyltransferase specific to Lys27 of histone H3.,Manzur KL, Farooq A, Zeng L, Plotnikova O, Koch AW, Sachchidanand, Zhou MM Nat Struct Biol. 2003 Mar;10(3):187-96. PMID:12567185<ref>PMID:12567185</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1n3j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Histone methyltransferase 3D structures\|Histone methyltransferase 3D structures]]		*[[Histone methyltransferase 3D structures\|Histone methyltransferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chlorella pbcv-1 virus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Farooq, A]]	+	[[Category: Paramecium bursaria Chlorella virus 1]]
-	[[Category: Koch~~, A W~~]]	+	[[Category: Farooq A]]
-	[[Category: Manzur~~, K L~~]]	+	[[Category: Koch AW]]
-	[[Category: Plotnikova, O]]	+	[[Category: Manzur KL]]
		+	[[Category: Plotnikova O]]
	[[Category: Sachchidanand]]		[[Category: Sachchidanand]]
-	[[Category: Zeng, L]]	+	[[Category: Zeng L]]
-	[[Category: Zhou, M M]]	+	[[Category: Zhou M-M]]
-	~~[[Category: Beta barrel]]~~	+
-	~~[[Category: Homodimer]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:20, 23 February 2022

OCA — Wed, 23 Feb 2022 08:20:35 GMT

←Older revision		Revision as of 08:20, 23 February 2022
Line 3:		Line 3:
	<StructureSection load='1n3j' size='340' side='right'caption='[[1n3j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1n3j' size='340' side='right'caption='[[1n3j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1N3J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N3J FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Chlorella PBCV-1 virus])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Chlorella PBCV-1 virus])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [~~http~~://pdbe.org/1n3j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1n3j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [https://pdbe.org/1n3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [https://www.ebi.ac.uk/pdbsum/1n3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:44, 2 December 2020

OCA — Wed, 02 Dec 2020 08:44:45 GMT

←Older revision		Revision as of 08:44, 2 December 2020
Line 1:		Line 1:

	==Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1==		==Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1==
-	<StructureSection load='1n3j' size='340' side='right' caption='[[1n3j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1n3j' size='340' side='right'caption='[[1n3j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1N3J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1N3J FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Chlorella PBCV-1 virus])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Chlorella PBCV-1 virus])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [http://pdbe.org/1n3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [http://www.ebi.ac.uk/pdbsum/1n3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [http://pdbe.org/1n3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [http://www.ebi.ac.uk/pdbsum/1n3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/1n3j_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/1n3j_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 26:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1n3j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1n3j" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Histone methyltransferase 3D structures\|Histone methyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Chlorella pbcv-1 virus]]		[[Category: Chlorella pbcv-1 virus]]
		+	[[Category: Large Structures]]
	[[Category: Farooq, A]]		[[Category: Farooq, A]]
	[[Category: Koch, A W]]		[[Category: Koch, A W]]

OCA at 10:17, 25 October 2017

OCA — Wed, 25 Oct 2017 10:17:12 GMT

←Older revision		Revision as of 10:17, 25 October 2017
Line 1:		Line 1:
		+
	==Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1==		==Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1==
	<StructureSection load='1n3j' size='340' side='right' caption='[[1n3j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1n3j' size='340' side='right' caption='[[1n3j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N3J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N3J FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Chlorella PBCV-1 virus])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Chlorella PBCV-1 virus])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [http://pdbe.org/1n3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [http://www.ebi.ac.uk/pdbsum/1n3j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [http://pdbe.org/1n3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [http://www.ebi.ac.uk/pdbsum/1n3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1n3j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1n3j" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Histone methyltransferase\|Histone methyltransferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:24, 11 September 2015

OCA — Fri, 11 Sep 2015 08:24:08 GMT

←Older revision		Revision as of 08:24, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1n3j' size='340' side='right' caption='[[1n3j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1n3j' size='340' side='right' caption='[[1n3j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus~~ 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N3J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N3J FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 ~~Paramecium bursaria~~ Chlorella ~~virus~~ 1])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Chlorella PBCV-1 virus])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [http://www.ebi.ac.uk/pdbsum/1n3j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [http://pdbe.org/1n3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [http://www.ebi.ac.uk/pdbsum/1n3j PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1n3j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Paramecium bursaria chlorella virus~~ 1]]	+	[[Category: Chlorella pbcv-1 virus]]
	[[Category: Farooq, A]]		[[Category: Farooq, A]]
	[[Category: Koch, A W]]		[[Category: Koch, A W]]

OCA at 16:34, 5 January 2015

OCA — Mon, 05 Jan 2015 16:34:22 GMT

←Older revision		Revision as of 16:34, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N3J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N3J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Paramecium bursaria Chlorella virus 1])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Paramecium bursaria Chlorella virus 1])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [http://www.ebi.ac.uk/pdbsum/1n3j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [http://www.ebi.ac.uk/pdbsum/1n3j PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Paramecium bursaria chlorella virus 1]]		[[Category: Paramecium bursaria chlorella virus 1]]
-	[[Category: Farooq, A.]]	+	[[Category: Farooq, A]]
-	[[Category: Koch, A W.]]	+	[[Category: Koch, A W]]
-	[[Category: Manzur, K L.]]	+	[[Category: Manzur, K L]]
-	[[Category: Plotnikova, O.]]	+	[[Category: Plotnikova, O]]
	[[Category: Sachchidanand]]		[[Category: Sachchidanand]]
-	[[Category: Zeng, L.]]	+	[[Category: Zeng, L]]
-	[[Category: Zhou, M M.]]	+	[[Category: Zhou, M M]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Homodimer]]		[[Category: Homodimer]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 15:59, 28 September 2014

OCA — Sun, 28 Sep 2014 15:59:45 GMT

←Older revision		Revision as of 15:59, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1n3j.~~png\|left~~\|200px]]	+	==Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1==
		+	<StructureSection load='1n3j' size='340' side='right' caption='[[1n3j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N3J FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Paramecium bursaria Chlorella virus 1])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [http://www.ebi.ac.uk/pdbsum/1n3j PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/1n3j_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Site-specific lysine methylation of histones by SET domains is a hallmark for epigenetic control of gene transcription in eukaryotic organisms. Here we report that a SET domain protein from Paramecium bursaria chlorella virus can specifically di-methylate Lys27 in histone H3, a modification implicated in gene silencing. The solution structure of the viral SET domain reveals a butterfly-shaped head-to-head symmetric dimer different from other known protein methyltransferases. Each subunit consists of a Greek-key antiparallel beta-barrel and a three-stranded open-faced sandwich that mediates the dimer interface. Cofactor S-adenosyl-L-methionine (SAM) binds at the opening of the beta-barrel, and amino acids C-terminal to Lys27 in H3 and in the flexible C-terminal tail of the enzyme confer the specificity of this viral histone methyltransferase.

-	~~{{STRUCTURE_1n3j\| PDB=1n3j \| SCENE= }}~~	+	A dimeric viral SET domain methyltransferase specific to Lys27 of histone H3.,Manzur KL, Farooq A, Zeng L, Plotnikova O, Koch AW, Sachchidanand, Zhou MM Nat Struct Biol. 2003 Mar;10(3):187-96. PMID:12567185<ref>PMID:12567185</ref>

-	~~===Structure and Substrate~~ of ~~a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12567185}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1n3j]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA].~~	+

	==See Also==		==See Also==
	*[[Histone methyltransferase\|Histone methyltransferase]]		*[[Histone methyltransferase\|Histone methyltransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012567185<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Paramecium bursaria chlorella virus 1]]		[[Category: Paramecium bursaria chlorella virus 1]]
	[[Category: Farooq, A.]]		[[Category: Farooq, A.]]

OCA at 17:26, 20 October 2012

OCA — Sat, 20 Oct 2012 17:26:59 GMT

←Older revision		Revision as of 17:26, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1n3j]] is a 2 chain structure ~~of [[Histone methyltransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA].	+	[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA].

	==See Also==		==See Also==

OCA: Protected "1n3j" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 00:55:33 GMT

Protected "1n3j" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:55, 27 July 2012