1n4p - Revision history

OCA at 07:52, 14 February 2024

OCA — Wed, 14 Feb 2024 07:52:40 GMT

←Older revision		Revision as of 07:52, 14 February 2024
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	<StructureSection load='1n4p' size='340' side='right'caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='1n4p' size='340' side='right'caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N4P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N4P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d8d\|1d8d]], [[1fpp\|1fpp]], [[1ft1\|1ft1]], [[1kzo\|1kzo]], [[1kzp\|1kzp]], [[1qbq\|1qbq]], [[1dce\|1dce]], [[1n4q\|1n4q]], [[1n4r\|1n4r]], [[1n4s\|1n4s]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_I Protein geranylgeranyltransferase type I], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.59 2.5.1.59] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [https://pdbe.org/1n4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [https://www.ebi.ac.uk/pdbsum/1n4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [https://pdbe.org/1n4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [https://www.ebi.ac.uk/pdbsum/1n4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FNTA_RAT FNTA_RAT]] Catalyzes the transfer of a farnesyl or geranyl-geranyl moiety from farnesyl or geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The alpha subunit is thought to participate in a stable complex with the substrate. The beta subunit binds the peptide substrate. Through RAC1 prenylation and activation may positively regulate neuromuscular junction development downstream of MUSK (By similarity). [[https://www.uniprot.org/uniprot/PGTB1_RAT PGTB1_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. Acts on the Rac1, Rac2, Rap1A and Rap1B proteins. The beta subunit is responsible for peptide-binding.	+	[https://www.uniprot.org/uniprot/FNTA_RAT FNTA_RAT] Catalyzes the transfer of a farnesyl or geranyl-geranyl moiety from farnesyl or geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The alpha subunit is thought to participate in a stable complex with the substrate. The beta subunit binds the peptide substrate. Through RAC1 prenylation and activation may positively regulate neuromuscular junction development downstream of MUSK (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n4p ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n4p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Protein geranylgeranyltransferase type-I (GGTase-I), one of two CaaX prenyltransferases, is an essential enzyme in eukaryotes. GGTase-I catalyzes C-terminal lipidation of >100 proteins, including many GTP- binding regulatory proteins. We present the first structural information for mammalian GGTase-I, including a series of substrate and product complexes that delineate the path of the chemical reaction. These structures reveal that all protein prenyltransferases share a common reaction mechanism and identify specific residues that play a dominant role in determining prenyl group specificity. This hypothesis was confirmed by converting farnesyltransferase (15-C prenyl substrate) into GGTase-I (20-C prenyl substrate) with a single point mutation. GGTase-I discriminates against farnesyl diphosphate (FPP) at the product turnover step through the inability of a 15-C FPP to displace the 20-C prenyl-peptide product. Understanding these key features of specificity is expected to contribute to optimization of anti-cancer and anti-parasite drugs.
-
-	Structure of mammalian protein geranylgeranyltransferase type-I.,Taylor JS, Reid TS, Terry KL, Casey PJ, Beese LS EMBO J. 2003 Nov 17;22(22):5963-74. PMID:14609943<ref>PMID:14609943</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1n4p" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Farnesyltransferase 3D structures\|Farnesyltransferase 3D structures]]		*[[Farnesyltransferase 3D structures\|Farnesyltransferase 3D structures]]
	*[[Geranylgeranyl transferase 3D structures\|Geranylgeranyl transferase 3D structures]]		*[[Geranylgeranyl transferase 3D structures\|Geranylgeranyl transferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Protein geranylgeranyltransferase type I~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: Beese~~, L S~~]]	+	[[Category: Beese LS]]
-	[[Category: Casey~~, P J~~]]	+	[[Category: Casey PJ]]
-	[[Category: Reid~~, T S~~]]	+	[[Category: Reid TS]]
-	[[Category: Taylor~~, J S]]~~	+	[[Category: Taylor JS]]
-	~~[[Category: Caax]]~~	+
-	~~[[Category: Geranylgeranyl]]~~	+
-	~~[[Category: Ggtase]]~~	+
-	~~[[Category: Lipid modification]]~~	+
-	~~[[Category: Protein geranylgeranyltransferase type-i]]~~	+
-	~~[[Category: Protein prenylation]]~~	+
-	~~[[Category: Rap2b]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:51, 12 May 2021

OCA — Wed, 12 May 2021 09:51:47 GMT

←Older revision		Revision as of 09:51, 12 May 2021
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	<StructureSection load='1n4p' size='340' side='right'caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='1n4p' size='340' side='right'caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1N4P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N4P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8d\|1d8d]], [[1fpp\|1fpp]], [[1ft1\|1ft1]], [[1kzo\|1kzo]], [[1kzp\|1kzp]], [[1qbq\|1qbq]], [[1dce\|1dce]], [[1n4q\|1n4q]], [[1n4r\|1n4r]], [[1n4s\|1n4s]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d8d\|1d8d]], [[1fpp\|1fpp]], [[1ft1\|1ft1]], [[1kzo\|1kzo]], [[1kzp\|1kzp]], [[1qbq\|1qbq]], [[1dce\|1dce]], [[1n4q\|1n4q]], [[1n4r\|1n4r]], [[1n4s\|1n4s]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_I Protein geranylgeranyltransferase type I], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.59 2.5.1.59] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_I Protein geranylgeranyltransferase type I], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.59 2.5.1.59] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [~~http~~://pdbe.org/1n4p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1n4p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [https://pdbe.org/1n4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [https://www.ebi.ac.uk/pdbsum/1n4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FNTA_RAT FNTA_RAT]] Catalyzes the transfer of a farnesyl or geranyl-geranyl moiety from farnesyl or geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The alpha subunit is thought to participate in a stable complex with the substrate. The beta subunit binds the peptide substrate. Through RAC1 prenylation and activation may positively regulate neuromuscular junction development downstream of MUSK (By similarity). [[~~http~~://www.uniprot.org/uniprot/PGTB1_RAT PGTB1_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. Acts on the Rac1, Rac2, Rap1A and Rap1B proteins. The beta subunit is responsible for peptide-binding.	+	[[https://www.uniprot.org/uniprot/FNTA_RAT FNTA_RAT]] Catalyzes the transfer of a farnesyl or geranyl-geranyl moiety from farnesyl or geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The alpha subunit is thought to participate in a stable complex with the substrate. The beta subunit binds the peptide substrate. Through RAC1 prenylation and activation may positively regulate neuromuscular junction development downstream of MUSK (By similarity). [[https://www.uniprot.org/uniprot/PGTB1_RAT PGTB1_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. Acts on the Rac1, Rac2, Rap1A and Rap1B proteins. The beta subunit is responsible for peptide-binding.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:43, 7 August 2019

OCA — Wed, 07 Aug 2019 06:43:42 GMT

←Older revision		Revision as of 06:43, 7 August 2019
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	==Protein Geranylgeranyltransferase type-I Complexed with Geranylgeranyl Diphosphate==		==Protein Geranylgeranyltransferase type-I Complexed with Geranylgeranyl Diphosphate==
-	<StructureSection load='1n4p' size='340' side='right' caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>	+	<StructureSection load='1n4p' size='340' side='right'caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N4P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N4P FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n4/1n4p_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n4/1n4p_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1n4p" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1n4p" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Farnesyltransferase 3D structures\|Farnesyltransferase 3D structures]]
		+	*[[Geranylgeranyl transferase 3D structures\|Geranylgeranyl transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Protein geranylgeranyltransferase type I]]		[[Category: Protein geranylgeranyltransferase type I]]
	[[Category: Beese, L S]]		[[Category: Beese, L S]]

OCA at 11:45, 6 November 2017

OCA — Mon, 06 Nov 2017 11:45:39 GMT

←Older revision		Revision as of 11:45, 6 November 2017
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1n4p" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1n4p" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Farnesyltransferase\|Farnesyltransferase]]
-	*[[Geranylgeranyl transferase\|Geranylgeranyl transferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:28, 3 August 2017

OCA — Thu, 03 Aug 2017 08:28:10 GMT

←Older revision		Revision as of 08:28, 3 August 2017
Line 1:		Line 1:
		+
	==Protein Geranylgeranyltransferase type-I Complexed with Geranylgeranyl Diphosphate==		==Protein Geranylgeranyltransferase type-I Complexed with Geranylgeranyl Diphosphate==
	<StructureSection load='1n4p' size='340' side='right' caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='1n4p' size='340' side='right' caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N4P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N4P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8d\|1d8d]], [[1fpp\|1fpp]], [[1ft1\|1ft1]], [[1kzo\|1kzo]], [[1kzp\|1kzp]], [[1qbq\|1qbq]], [[1dce\|1dce]], [[1n4q\|1n4q]], [[1n4r\|1n4r]], [[1n4s\|1n4s]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8d\|1d8d]], [[1fpp\|1fpp]], [[1ft1\|1ft1]], [[1kzo\|1kzo]], [[1kzp\|1kzp]], [[1qbq\|1qbq]], [[1dce\|1dce]], [[1n4q\|1n4q]], [[1n4r\|1n4r]], [[1n4s\|1n4s]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [http://pdbe.org/1n4p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [http://www.ebi.ac.uk/pdbsum/1n4p PDBsum]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_geranylgeranyltransferase_type_I Protein geranylgeranyltransferase type I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.59 2.5.1.59] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [http://pdbe.org/1n4p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [http://www.ebi.ac.uk/pdbsum/1n4p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 36:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Buffalo rat~~]]	+	[[Category: Protein geranylgeranyltransferase type I]]
	[[Category: Beese, L S]]		[[Category: Beese, L S]]
	[[Category: Casey, P J]]		[[Category: Casey, P J]]

OCA at 18:14, 7 February 2016

OCA — Sun, 07 Feb 2016 18:14:08 GMT

←Older revision		Revision as of 18:14, 7 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n4p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:56, 10 September 2015

OCA — Thu, 10 Sep 2015 03:56:51 GMT

←Older revision		Revision as of 03:56, 10 September 2015
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	<StructureSection load='1n4p' size='340' side='right' caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='1n4p' size='340' side='right' caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N4P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N4P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8d\|1d8d]], [[1fpp\|1fpp]], [[1ft1\|1ft1]], [[1kzo\|1kzo]], [[1kzp\|1kzp]], [[1qbq\|1qbq]], [[1dce\|1dce]], [[1n4q\|1n4q]], [[1n4r\|1n4r]], [[1n4s\|1n4s]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8d\|1d8d]], [[1fpp\|1fpp]], [[1ft1\|1ft1]], [[1kzo\|1kzo]], [[1kzp\|1kzp]], [[1qbq\|1qbq]], [[1dce\|1dce]], [[1n4q\|1n4q]], [[1n4r\|1n4r]], [[1n4s\|1n4s]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [http://www.ebi.ac.uk/pdbsum/1n4p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [http://pdbe.org/1n4p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [http://www.ebi.ac.uk/pdbsum/1n4p PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1n4p" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rattus norvegicus~~]]	+	[[Category: Buffalo rat]]
	[[Category: Beese, L S]]		[[Category: Beese, L S]]
	[[Category: Casey, P J]]		[[Category: Casey, P J]]

OCA at 17:21, 24 December 2014

OCA — Wed, 24 Dec 2014 17:21:21 GMT

←Older revision		Revision as of 17:21, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N4P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N4P FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8d\|1d8d]], [[1fpp\|1fpp]], [[1ft1\|1ft1]], [[1kzo\|1kzo]], [[1kzp\|1kzp]], [[1qbq\|1qbq]], [[1dce\|1dce]], [[1n4q\|1n4q]], [[1n4r\|1n4r]], [[1n4s\|1n4s]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8d\|1d8d]], [[1fpp\|1fpp]], [[1ft1\|1ft1]], [[1kzo\|1kzo]], [[1kzp\|1kzp]], [[1qbq\|1qbq]], [[1dce\|1dce]], [[1n4q\|1n4q]], [[1n4r\|1n4r]], [[1n4s\|1n4s]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [http://www.ebi.ac.uk/pdbsum/1n4p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [http://www.ebi.ac.uk/pdbsum/1n4p PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FNTA_RAT FNTA_RAT]] Catalyzes the transfer of a farnesyl or geranyl-geranyl moiety from farnesyl or geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The alpha subunit is thought to participate in a stable complex with the substrate. The beta subunit binds the peptide substrate. Through RAC1 prenylation and activation may positively regulate neuromuscular junction development downstream of MUSK (By similarity). [[http://www.uniprot.org/uniprot/PGTB1_RAT PGTB1_RAT]] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. Acts on the Rac1, Rac2, Rap1A and Rap1B proteins. The beta subunit is responsible for peptide-binding.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
-	[[Category: Beese, L S.]]	+	[[Category: Beese, L S]]
-	[[Category: Casey, P J.]]	+	[[Category: Casey, P J]]
-	[[Category: Reid, T S.]]	+	[[Category: Reid, T S]]
-	[[Category: Taylor, J S.]]	+	[[Category: Taylor, J S]]
	[[Category: Caax]]		[[Category: Caax]]
	[[Category: Geranylgeranyl]]		[[Category: Geranylgeranyl]]

OCA at 13:08, 28 September 2014

OCA — Sun, 28 Sep 2014 13:08:38 GMT

←Older revision		Revision as of 13:08, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1n4p.~~png~~\|~~left~~\|200px]]	+	==Protein Geranylgeranyltransferase type-I Complexed with Geranylgeranyl Diphosphate==
		+	<StructureSection load='1n4p' size='340' side='right' caption='[[1n4p]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1n4p]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N4P FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8d\|1d8d]], [[1fpp\|1fpp]], [[1ft1\|1ft1]], [[1kzo\|1kzo]], [[1kzp\|1kzp]], [[1qbq\|1qbq]], [[1dce\|1dce]], [[1n4q\|1n4q]], [[1n4r\|1n4r]], [[1n4s\|1n4s]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n4p RCSB], [http://www.ebi.ac.uk/pdbsum/1n4p PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n4/1n4p_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein geranylgeranyltransferase type-I (GGTase-I), one of two CaaX prenyltransferases, is an essential enzyme in eukaryotes. GGTase-I catalyzes C-terminal lipidation of >100 proteins, including many GTP- binding regulatory proteins. We present the first structural information for mammalian GGTase-I, including a series of substrate and product complexes that delineate the path of the chemical reaction. These structures reveal that all protein prenyltransferases share a common reaction mechanism and identify specific residues that play a dominant role in determining prenyl group specificity. This hypothesis was confirmed by converting farnesyltransferase (15-C prenyl substrate) into GGTase-I (20-C prenyl substrate) with a single point mutation. GGTase-I discriminates against farnesyl diphosphate (FPP) at the product turnover step through the inability of a 15-C FPP to displace the 20-C prenyl-peptide product. Understanding these key features of specificity is expected to contribute to optimization of anti-cancer and anti-parasite drugs.

-	~~{{STRUCTURE_1n4p\| PDB=1n4p \| SCENE= }}~~	+	Structure of mammalian protein geranylgeranyltransferase type-I.,Taylor JS, Reid TS, Terry KL, Casey PJ, Beese LS EMBO J. 2003 Nov 17;22(22):5963-74. PMID:14609943<ref>PMID:14609943</ref>

-	~~===Protein Geranylgeranyltransferase type-I Complexed with Geranylgeranyl Diphosphate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_14609943}}~~	+	==See Also==
-		+	*[[Farnesyltransferase\|Farnesyltransferase]]
-	==~~About this Structure~~==	+	*[[Geranylgeranyl transferase\|Geranylgeranyl transferase]]
-	[[~~1n4p~~]] ~~is a 14 chain structure with sequence from~~ [~~http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus~~]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4P OCA~~].	+	== References ==
-		+	<references/>
-	==~~Reference~~==	+	__TOC__
-	<~~ref group="xtra">PMID:014609943<~~/~~ref~~><~~references group="xtra"~~/>	+	</StructureSection>
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
	[[Category: Beese, L S.]]		[[Category: Beese, L S.]]

OCA: Protected "1n4p" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 19:43:37 GMT

Protected "1n4p" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:43, 5 December 2012