1n7o - Revision history

OCA at 07:53, 14 February 2024

OCA — Wed, 14 Feb 2024 07:53:44 GMT

←Older revision		Revision as of 07:53, 14 February 2024
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	<StructureSection load='1n7o' size='340' side='right'caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1n7o' size='340' side='right'caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus~~ pneumoniae~~" (klein 1884) weichselbaum 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N7O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N7O FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1c82\|1c82]]~~, [[~~1n7n~~\|~~1n7n~~]]~~, [[1n7p\|1n7p]], [[1n7q\|1n7q]], [[1n7r\|1n7r]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.~~2.2.1] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [https://pdbe.org/1n7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [https://www.ebi.ac.uk/pdbsum/1n7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [https://pdbe.org/1n7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [https://www.ebi.ac.uk/pdbsum/1n7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7o ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HYSA_STRPN HYSA_STRPN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n7o ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n7o ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Streptococcus pneumoniae hyaluronate lyase is a surface antigen of this Gram-positive human bacterial pathogen. The primary function of this enzyme is the degradation of hyaluronan, which is a major component of the extracellular matrix of the tissues of vertebrates and of some bacteria. The enzyme degrades its substrate through a beta-elimination process called proton acceptance and donation. The inherent part of this degradation is a processive mode of action of the enzyme degrading hyaluronan into unsaturated disaccharide hyaluronic acid blocks from the reducing to the nonreducing end of the polymer following the initial random endolytic binding to the substrate. The final degradation product is the unsaturated disaccharide hyaluronic acid. The residues of the enzyme that are involved in various aspects of such degradation were identified based on the three-dimensional structures of the native enzyme and its complexes with hyaluronan substrates of various lengths. The catalytic residues were identified to be Asn(349), His(399), and Tyr(408). The residues responsible for the release of the product of the reaction were identified as Glu(388), Asp(398), and Thr(400), and they were termed negative patch. The hydrophobic residues Trp(291), Trp(292), and Phe(343) were found to be responsible for the precise positioning of the substrate for enzyme catalysis and named hydrophobic patch. The comparison of the specific activities and kinetic properties of the wild type and the mutant enzymes involving the hydrophobic patch residues W292A, F343V, W291A/W292A, W292A/F343V, and W291A/W292A/F343V allowed for the characterization of every mutant and for the correlation of the activity and kinetic properties of the enzyme with its structure as well as the mechanism of catalysis.
-
-	The function of hydrophobic residues in the catalytic cleft of Streptococcus pneumoniae hyaluronate lyase. Kinetic characterization of mutant enzyme forms.,Nukui M, Taylor KB, McPherson DT, Shigenaga MK, Jedrzejas MJ J Biol Chem. 2003 Jan 31;278(5):3079-88. Epub 2002 Nov 21. PMID:12446724<ref>PMID:12446724</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1n7o" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Hyaluronidase 3D structures\|Hyaluronidase 3D structures]]		*[[Hyaluronidase 3D structures\|Hyaluronidase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Hyaluronate lyase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Jedrzejas, M J~~]]	+	[[Category: Streptococcus pneumoniae]]
-	[[Category: ~~McPherson, D T~~]]	+	[[Category: Jedrzejas MJ]]
-	[[Category: ~~Nukui, M~~]]	+	[[Category: McPherson DT]]
-	[[Category: ~~Shigenaga,~~ M]]	+	[[Category: Nukui M]]
-	[[Category: ~~Taylor, K B~~]]	+	[[Category: Shigenaga M]]
-	[[Category: ~~Lyase]]~~	+	[[Category: Taylor KB]]
-	~~[[Category: Protein mutant~~]]	+

OCA at 08:56, 21 April 2021

OCA — Wed, 21 Apr 2021 08:56:03 GMT

←Older revision		Revision as of 08:56, 21 April 2021
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	<StructureSection load='1n7o' size='340' side='right'caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1n7o' size='340' side='right'caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1N7O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N7O FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c82\|1c82]], [[1n7n\|1n7n]], [[1n7p\|1n7p]], [[1n7q\|1n7q]], [[1n7r\|1n7r]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1c82\|1c82]], [[1n7n\|1n7n]], [[1n7p\|1n7p]], [[1n7q\|1n7q]], [[1n7r\|1n7r]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [~~http~~://pdbe.org/1n7o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1n7o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7o ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [https://pdbe.org/1n7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [https://www.ebi.ac.uk/pdbsum/1n7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7o ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Hyaluronidase\|Hyaluronidase]]	+	*[[Hyaluronidase 3D structures\|Hyaluronidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:36, 17 July 2019

OCA — Wed, 17 Jul 2019 13:36:31 GMT

←Older revision		Revision as of 13:36, 17 July 2019
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	==Streptococcus pneumoniae Hyaluronate Lyase F343V Mutant==		==Streptococcus pneumoniae Hyaluronate Lyase F343V Mutant==
-	<StructureSection load='1n7o' size='340' side='right' caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='1n7o' size='340' side='right'caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N7O FirstGlance]. <br>		<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N7O FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/1n7o_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/1n7o_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 1n7o" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1n7o" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Hyaluronidase\|Hyaluronidase]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Hyaluronate lyase]]		[[Category: Hyaluronate lyase]]
		+	[[Category: Large Structures]]
	[[Category: Jedrzejas, M J]]		[[Category: Jedrzejas, M J]]
	[[Category: McPherson, D T]]		[[Category: McPherson, D T]]

OCA at 11:52, 12 October 2017

OCA — Thu, 12 Oct 2017 11:52:00 GMT

←Older revision		Revision as of 11:52, 12 October 2017
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		+
	==Streptococcus pneumoniae Hyaluronate Lyase F343V Mutant==		==Streptococcus pneumoniae Hyaluronate Lyase F343V Mutant==
	<StructureSection load='1n7o' size='340' side='right' caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1n7o' size='340' side='right' caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c82\|1c82]], [[1n7n\|1n7n]], [[1n7p\|1n7p]], [[1n7q\|1n7q]], [[1n7r\|1n7r]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c82\|1c82]], [[1n7n\|1n7n]], [[1n7p\|1n7p]], [[1n7q\|1n7q]], [[1n7r\|1n7r]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [http://pdbe.org/1n7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [http://www.ebi.ac.uk/pdbsum/1n7o PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [http://pdbe.org/1n7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [http://www.ebi.ac.uk/pdbsum/1n7o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7o ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n7o ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 1n7o" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1n7o" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Hyaluronidase\|Hyaluronidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:01, 12 September 2015

OCA — Sat, 12 Sep 2015 01:01:26 GMT

←Older revision		Revision as of 01:01, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1n7o' size='340' side='right' caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1n7o' size='340' side='right' caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_pneumoniae Streptococcus~~ pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N7O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N7O FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c82\|1c82]], [[1n7n\|1n7n]], [[1n7p\|1n7p]], [[1n7q\|1n7q]], [[1n7r\|1n7r]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c82\|1c82]], [[1n7n\|1n7n]], [[1n7p\|1n7p]], [[1n7q\|1n7q]], [[1n7r\|1n7r]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [http://www.ebi.ac.uk/pdbsum/1n7o PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [http://pdbe.org/1n7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [http://www.ebi.ac.uk/pdbsum/1n7o PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1n7o" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	</StructureSection>		</StructureSection>
	[[Category: Hyaluronate lyase]]		[[Category: Hyaluronate lyase]]
-	[[Category: Streptococcus pneumoniae]]
	[[Category: Jedrzejas, M J]]		[[Category: Jedrzejas, M J]]
	[[Category: McPherson, D T]]		[[Category: McPherson, D T]]

OCA at 17:03, 5 January 2015

OCA — Mon, 05 Jan 2015 17:03:04 GMT

←Older revision		Revision as of 17:03, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N7O FirstGlance]. <br>		<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N7O FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c82\|1c82]], [[1n7n\|1n7n]], [[1n7p\|1n7p]], [[1n7q\|1n7q]], [[1n7r\|1n7r]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c82\|1c82]], [[1n7n\|1n7n]], [[1n7p\|1n7p]], [[1n7q\|1n7q]], [[1n7r\|1n7r]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [http://www.ebi.ac.uk/pdbsum/1n7o PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [http://www.ebi.ac.uk/pdbsum/1n7o PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Hyaluronate lyase]]		[[Category: Hyaluronate lyase]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
-	[[Category: Jedrzejas, M J.]]	+	[[Category: Jedrzejas, M J]]
-	[[Category: McPherson, D T.]]	+	[[Category: McPherson, D T]]
-	[[Category: Nukui, M.]]	+	[[Category: Nukui, M]]
-	[[Category: Shigenaga, M.]]	+	[[Category: Shigenaga, M]]
-	[[Category: Taylor, K B.]]	+	[[Category: Taylor, K B]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Protein mutant]]		[[Category: Protein mutant]]

OCA at 16:15, 28 September 2014

OCA — Sun, 28 Sep 2014 16:15:04 GMT

←Older revision		Revision as of 16:15, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1n7o.~~png~~\|~~left~~\|200px]]	+	==Streptococcus pneumoniae Hyaluronate Lyase F343V Mutant==
		+	<StructureSection load='1n7o' size='340' side='right' caption='[[1n7o]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1n7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N7O FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c82\|1c82]], [[1n7n\|1n7n]], [[1n7p\|1n7p]], [[1n7q\|1n7q]], [[1n7r\|1n7r]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n7o RCSB], [http://www.ebi.ac.uk/pdbsum/1n7o PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/1n7o_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Streptococcus pneumoniae hyaluronate lyase is a surface antigen of this Gram-positive human bacterial pathogen. The primary function of this enzyme is the degradation of hyaluronan, which is a major component of the extracellular matrix of the tissues of vertebrates and of some bacteria. The enzyme degrades its substrate through a beta-elimination process called proton acceptance and donation. The inherent part of this degradation is a processive mode of action of the enzyme degrading hyaluronan into unsaturated disaccharide hyaluronic acid blocks from the reducing to the nonreducing end of the polymer following the initial random endolytic binding to the substrate. The final degradation product is the unsaturated disaccharide hyaluronic acid. The residues of the enzyme that are involved in various aspects of such degradation were identified based on the three-dimensional structures of the native enzyme and its complexes with hyaluronan substrates of various lengths. The catalytic residues were identified to be Asn(349), His(399), and Tyr(408). The residues responsible for the release of the product of the reaction were identified as Glu(388), Asp(398), and Thr(400), and they were termed negative patch. The hydrophobic residues Trp(291), Trp(292), and Phe(343) were found to be responsible for the precise positioning of the substrate for enzyme catalysis and named hydrophobic patch. The comparison of the specific activities and kinetic properties of the wild type and the mutant enzymes involving the hydrophobic patch residues W292A, F343V, W291A/W292A, W292A/F343V, and W291A/W292A/F343V allowed for the characterization of every mutant and for the correlation of the activity and kinetic properties of the enzyme with its structure as well as the mechanism of catalysis.

-	~~{{STRUCTURE_1n7o\| PDB=1n7o \| SCENE= }}~~	+	The function of hydrophobic residues in the catalytic cleft of Streptococcus pneumoniae hyaluronate lyase. Kinetic characterization of mutant enzyme forms.,Nukui M, Taylor KB, McPherson DT, Shigenaga MK, Jedrzejas MJ J Biol Chem. 2003 Jan 31;278(5):3079-88. Epub 2002 Nov 21. PMID:12446724<ref>PMID:12446724</ref>

-	~~===Streptococcus pneumoniae Hyaluronate Lyase F343V Mutant===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12446724}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1n7o]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA].~~	+

	==See Also==		==See Also==
	*[[Hyaluronidase\|Hyaluronidase]]		*[[Hyaluronidase\|Hyaluronidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012446724<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Hyaluronate lyase]]		[[Category: Hyaluronate lyase]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]

OCA at 22:01, 20 October 2012

OCA — Sat, 20 Oct 2012 22:01:30 GMT

←Older revision		Revision as of 22:01, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1n7o]] is a 1 chain structure ~~of [[Hyaluronidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA].	+	[[1n7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA].

	==See Also==		==See Also==

OCA: Protected "1n7o" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 15:00:19 GMT

Protected "1n7o" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:00, 27 July 2012

OCA at 15:00, 27 July 2012

OCA — Fri, 27 Jul 2012 15:00:17 GMT

←Older revision		Revision as of 15:00, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1n7o.png\|left\|200px]]		[[Image:1n7o.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1n7o", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1n7o\| PDB=1n7o \| SCENE= }}		{{STRUCTURE_1n7o\| PDB=1n7o \| SCENE= }}

	===Streptococcus pneumoniae Hyaluronate Lyase F343V Mutant===		===Streptococcus pneumoniae Hyaluronate Lyase F343V Mutant===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12446724}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12446724 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12446724}}		{{ABSTRACT_PUBMED_12446724}}

	==About this Structure==		==About this Structure==
-	~~1N7O~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA].	+	[[1n7o]] is a 1 chain structure of [[Hyaluronidase]] with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7O OCA].
		+
		+	==See Also==
		+	*[[Hyaluronidase\|Hyaluronidase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12446724~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012446724</ref><references group="xtra"/>
	[[Category: Hyaluronate lyase]]		[[Category: Hyaluronate lyase]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
Line 31:		Line 22:
	[[Category: Shigenaga, M.]]		[[Category: Shigenaga, M.]]
	[[Category: Taylor, K B.]]		[[Category: Taylor, K B.]]
		+	[[Category: Lyase]]
	[[Category: Protein mutant]]		[[Category: Protein mutant]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:50:44 2009''