1neb - Revision history

OCA at 08:53, 22 May 2024

OCA — Wed, 22 May 2024 08:53:33 GMT

←Older revision		Revision as of 08:53, 22 May 2024
Line 21:		Line 21:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1neb ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1neb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The huge modular protein nebulin is located in the thin filament of striated muscle in vertebrates and is thought to bind and stabilize F-actin. The C-terminal part of human nebulin is anchored in the sarcomeric Z-disk and contains an SH3 domain, the first of such motifs to be identified in a myofibrillar protein. We have determined the nebulin SH3 sequence from several species and found it strikingly conserved. We have also shown that the SH3 transcripts are constitutively expressed in skeletal muscle tissues. As the first step towards a molecular understanding of nebulin's cellular role we have determined the three-dimensional structure of the human nebulin SH3 domain in solution by nuclear magnetic resonance (NMR) spectroscopy and compared it with other known SH3 structures. The nebulin SH3 domain has a well-defined structure in solution with a typical SH3 topology, consisting of a beta-sandwich of two triple-stranded, antiparallel beta-sheets arranged at right angles to each other and of a single turn of a 310-helix. An additional double-stranded antiparallel beta-sheet in the RT loop bends over the beta-sandwich. The derived structure reveals a remarkable similarity with a distinct subset of SH3 domains, especially in the structural features of the exposed hydrophobic patch that is thought to be the site of interaction with polyproline ligands. On the basis of this similarity, we have modelled the interaction with an appropriate polyproline ligand and attempted to delineate the characteristics of the physiological SH3-binding partner in the Z-disk. Our results represent the first step in reconstructing the structure of nebulin and are expected to contribute to our understanding of nebulin's functional role in myofibrillar assembly.
		+
		+	SH3 in muscles: solution structure of the SH3 domain from nebulin.,Politou AS, Millevoi S, Gautel M, Kolmerer B, Pastore A J Mol Biol. 1998 Feb 13;276(1):189-202. PMID:9514727<ref>PMID:9514727</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1neb" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 08:48, 10 April 2024

OCA — Wed, 10 Apr 2024 08:48:42 GMT

←Older revision		Revision as of 08:48, 10 April 2024
Line 1:		Line 1:

	==SH3 DOMAIN FROM HUMAN NEBULIN, NMR, MINIMIZED AVERAGE STRUCTURE==		==SH3 DOMAIN FROM HUMAN NEBULIN, NMR, MINIMIZED AVERAGE STRUCTURE==
-	<StructureSection load='1neb' size='340' side='right'caption='[[1neb~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1neb' size='340' side='right'caption='[[1neb]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NEB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NEB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [https://pdbe.org/1neb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [https://www.ebi.ac.uk/pdbsum/1neb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1neb ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [https://pdbe.org/1neb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [https://www.ebi.ac.uk/pdbsum/1neb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1neb ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:[https://omim.org/entry/256030 256030]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.<ref>PMID:10051637</ref>	+	[https://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN] Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:[https://omim.org/entry/256030 256030]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.<ref>PMID:10051637</ref>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.	+	[https://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN] This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 21:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1neb ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1neb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The huge modular protein nebulin is located in the thin filament of striated muscle in vertebrates and is thought to bind and stabilize F-actin. The C-terminal part of human nebulin is anchored in the sarcomeric Z-disk and contains an SH3 domain, the first of such motifs to be identified in a myofibrillar protein. We have determined the nebulin SH3 sequence from several species and found it strikingly conserved. We have also shown that the SH3 transcripts are constitutively expressed in skeletal muscle tissues. As the first step towards a molecular understanding of nebulin's cellular role we have determined the three-dimensional structure of the human nebulin SH3 domain in solution by nuclear magnetic resonance (NMR) spectroscopy and compared it with other known SH3 structures. The nebulin SH3 domain has a well-defined structure in solution with a typical SH3 topology, consisting of a beta-sandwich of two triple-stranded, antiparallel beta-sheets arranged at right angles to each other and of a single turn of a 310-helix. An additional double-stranded antiparallel beta-sheet in the RT loop bends over the beta-sandwich. The derived structure reveals a remarkable similarity with a distinct subset of SH3 domains, especially in the structural features of the exposed hydrophobic patch that is thought to be the site of interaction with polyproline ligands. On the basis of this similarity, we have modelled the interaction with an appropriate polyproline ligand and attempted to delineate the characteristics of the physiological SH3-binding partner in the Z-disk. Our results represent the first step in reconstructing the structure of nebulin and are expected to contribute to our understanding of nebulin's functional role in myofibrillar assembly.
-
-	SH3 in muscles: solution structure of the SH3 domain from nebulin.,Politou AS, Millevoi S, Gautel M, Kolmerer B, Pastore A J Mol Biol. 1998 Feb 13;276(1):189-202. PMID:9514727<ref>PMID:9514727</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1neb" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pastore, A]]	+	[[Category: Pastore A]]
-	[[Category: Politou~~, A S]]~~	+	[[Category: Politou AS]]
-	~~[[Category: Actin-binding]]~~	+
-	~~[[Category: Nebulin]]~~	+
-	~~[[Category: Sh3 domain]]~~	+
-	~~[[Category: Z-disk~~]]	+

OCA at 07:01, 25 August 2021

OCA — Wed, 25 Aug 2021 07:01:46 GMT

←Older revision		Revision as of 07:01, 25 August 2021
Line 3:		Line 3:
	<StructureSection load='1neb' size='340' side='right'caption='[[1neb]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1neb' size='340' side='right'caption='[[1neb]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1NEB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NEB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [~~http~~://pdbe.org/1neb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1neb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1neb ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [https://pdbe.org/1neb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [https://www.ebi.ac.uk/pdbsum/1neb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1neb ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:[~~http~~://omim.org/entry/256030 256030]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.<ref>PMID:10051637</ref>	+	[[https://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:[https://omim.org/entry/256030 256030]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.<ref>PMID:10051637</ref>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.	+	[[https://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:52, 20 November 2019

OCA — Wed, 20 Nov 2019 18:52:49 GMT

←Older revision		Revision as of 18:52, 20 November 2019
Line 1:		Line 1:

	==SH3 DOMAIN FROM HUMAN NEBULIN, NMR, MINIMIZED AVERAGE STRUCTURE==		==SH3 DOMAIN FROM HUMAN NEBULIN, NMR, MINIMIZED AVERAGE STRUCTURE==
-	<StructureSection load='1neb' size='340' side='right' caption='[[1neb]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1neb' size='340' side='right'caption='[[1neb]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEB FirstGlance]. <br>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1neb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1neb" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nebulin\|Nebulin]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Pastore, A]]		[[Category: Pastore, A]]
	[[Category: Politou, A S]]		[[Category: Politou, A S]]

OCA at 07:38, 7 February 2018

OCA — Wed, 07 Feb 2018 07:38:22 GMT

←Older revision		Revision as of 07:38, 7 February 2018
Line 1:		Line 1:
		+
	==SH3 DOMAIN FROM HUMAN NEBULIN, NMR, MINIMIZED AVERAGE STRUCTURE==		==SH3 DOMAIN FROM HUMAN NEBULIN, NMR, MINIMIZED AVERAGE STRUCTURE==
	<StructureSection load='1neb' size='340' side='right' caption='[[1neb]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1neb' size='340' side='right' caption='[[1neb]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [http://pdbe.org/1neb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [http://www.ebi.ac.uk/pdbsum/1neb PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [http://pdbe.org/1neb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [http://www.ebi.ac.uk/pdbsum/1neb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1neb ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ne/1neb_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ne/1neb_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1neb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1neb" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Nebulin\|Nebulin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:50, 8 February 2016

OCA — Mon, 08 Feb 2016 15:50:15 GMT

←Older revision		Revision as of 15:50, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='1neb' size='340' side='right' caption='[[1neb]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1neb' size='340' side='right' caption='[[1neb]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [http://www.ebi.ac.uk/pdbsum/1neb PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [http://pdbe.org/1neb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [http://www.ebi.ac.uk/pdbsum/1neb PDBsum]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1neb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1neb" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Pastore, A]]		[[Category: Pastore, A]]
	[[Category: Politou, A S]]		[[Category: Politou, A S]]

OCA at 09:35, 1 July 2015

OCA — Wed, 01 Jul 2015 09:35:02 GMT

←Older revision		Revision as of 09:35, 1 July 2015
Line 40:		Line 40:
	[[Category: Nebulin]]		[[Category: Nebulin]]
	[[Category: Sh3 domain]]		[[Category: Sh3 domain]]
-	[[Category: Z-disk ~~assembly~~]]	+	[[Category: Z-disk]]

OCA at 17:11, 5 January 2015

OCA — Mon, 05 Jan 2015 17:11:40 GMT

←Older revision		Revision as of 17:11, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEB FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [http://www.ebi.ac.uk/pdbsum/1neb PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [http://www.ebi.ac.uk/pdbsum/1neb PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:[http://omim.org/entry/256030 256030]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.<ref>PMID:10051637</ref>		[[http://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:[http://omim.org/entry/256030 256030]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.<ref>PMID:10051637</ref>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Pastore, A.]]	+	[[Category: Pastore, A]]
-	[[Category: Politou, A S.]]	+	[[Category: Politou, A S]]
	[[Category: Actin-binding]]		[[Category: Actin-binding]]
	[[Category: Nebulin]]		[[Category: Nebulin]]
	[[Category: Sh3 domain]]		[[Category: Sh3 domain]]
	[[Category: Z-disk assembly]]		[[Category: Z-disk assembly]]

OCA at 15:48, 29 September 2014

OCA — Mon, 29 Sep 2014 15:48:05 GMT

←Older revision		Revision as of 15:48, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1neb\| PDB=1neb \| SCENE= }}~~	+	==SH3 DOMAIN FROM HUMAN NEBULIN, NMR, MINIMIZED AVERAGE STRUCTURE==
-	===SH3 DOMAIN FROM HUMAN NEBULIN, NMR, MINIMIZED AVERAGE STRUCTURE===	+	<StructureSection load='1neb' size='340' side='right' caption='[[1neb]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_9514727}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1neb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEB FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1neb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1neb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1neb RCSB], [http://www.ebi.ac.uk/pdbsum/1neb PDBsum]</span></td></tr>
		+	<table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:[http://omim.org/entry/256030 256030]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.<ref>PMID:10051637</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ne/1neb_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The huge modular protein nebulin is located in the thin filament of striated muscle in vertebrates and is thought to bind and stabilize F-actin. The C-terminal part of human nebulin is anchored in the sarcomeric Z-disk and contains an SH3 domain, the first of such motifs to be identified in a myofibrillar protein. We have determined the nebulin SH3 sequence from several species and found it strikingly conserved. We have also shown that the SH3 transcripts are constitutively expressed in skeletal muscle tissues. As the first step towards a molecular understanding of nebulin's cellular role we have determined the three-dimensional structure of the human nebulin SH3 domain in solution by nuclear magnetic resonance (NMR) spectroscopy and compared it with other known SH3 structures. The nebulin SH3 domain has a well-defined structure in solution with a typical SH3 topology, consisting of a beta-sandwich of two triple-stranded, antiparallel beta-sheets arranged at right angles to each other and of a single turn of a 310-helix. An additional double-stranded antiparallel beta-sheet in the RT loop bends over the beta-sandwich. The derived structure reveals a remarkable similarity with a distinct subset of SH3 domains, especially in the structural features of the exposed hydrophobic patch that is thought to be the site of interaction with polyproline ligands. On the basis of this similarity, we have modelled the interaction with an appropriate polyproline ligand and attempted to delineate the characteristics of the physiological SH3-binding partner in the Z-disk. Our results represent the first step in reconstructing the structure of nebulin and are expected to contribute to our understanding of nebulin's functional role in myofibrillar assembly.

-	~~==Disease==~~	+	SH3 in muscles: solution structure of the SH3 domain from nebulin.,Politou AS, Millevoi S, Gautel M, Kolmerer B, Pastore A J Mol Biol. 1998 Feb 13;276(1):189-202. PMID:9514727<ref>PMID:9514727</ref>
-	~~[[http~~:~~//www~~.~~uniprot~~.~~org/uniprot/NEBU_HUMAN NEBU_HUMAN]] Defects in NEB are the cause of nemaline myopathy type 2~~ (~~NEM2~~) ~~[MIM~~:~~[http://omim.org/entry/256030 256030]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread~~-~~or rod-like structures in muscle fibers on histologic examination~~.<ref>PMID:~~10051637~~</ref>	+
-		+
-	~~==Function==~~	+
-	[[http://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[1neb]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1NEB OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:009514727</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Nebulin\|Nebulin]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Pastore, A.]]		[[Category: Pastore, A.]]

OCA at 18:02, 24 March 2013

OCA — Sun, 24 Mar 2013 18:02:18 GMT

←Older revision		Revision as of 18:02, 24 March 2013
Line 1:		Line 1:
-	[[Image:1neb.png\|left\|200px]]
-
	{{STRUCTURE_1neb\| PDB=1neb \| SCENE= }}		{{STRUCTURE_1neb\| PDB=1neb \| SCENE= }}
-
	===SH3 DOMAIN FROM HUMAN NEBULIN, NMR, MINIMIZED AVERAGE STRUCTURE===		===SH3 DOMAIN FROM HUMAN NEBULIN, NMR, MINIMIZED AVERAGE STRUCTURE===
		+	{{ABSTRACT_PUBMED_9514727}}

-	~~{{ABSTRACT_PUBMED_9514727}}~~	+	==Disease==
		+	[[http://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:[http://omim.org/entry/256030 256030]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.<ref>PMID:10051637</ref>
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/NEBU_HUMAN NEBU_HUMAN]] This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.

	==About this Structure==		==About this Structure==
Line 11:		Line 13:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:009514727</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009514727</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Pastore, A.]]		[[Category: Pastore, A.]]