1nev - Revision history

OCA at 18:53, 29 November 2023

OCA — Wed, 29 Nov 2023 18:53:13 GMT

←Older revision		Revision as of 18:53, 29 November 2023
Line 1:		Line 1:

	==A-tract decamer==		==A-tract decamer==
-	<StructureSection load='1nev' size='340' side='right'caption='[[1nev~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='1nev' size='340' side='right'caption='[[1nev]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NEV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [https://pdbe.org/1nev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [https://www.ebi.ac.uk/pdbsum/1nev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nev ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [https://pdbe.org/1nev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [https://www.ebi.ac.uk/pdbsum/1nev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nev ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Barbic, A]]	+	[[Category: Barbic A]]
-	[[Category: Crothers~~, D M~~]]	+	[[Category: Crothers DM]]
-	[[Category: Zimmer~~, D P]]~~	+	[[Category: Zimmer DP]]
-	~~[[Category: A-tract]]~~	+
-	~~[[Category: Dna]]~~	+
-	~~[[Category: Dna bending]]~~	+
-	~~[[Category: Dna decamer~~]]	+

OCA at 09:34, 5 May 2021

OCA — Wed, 05 May 2021 09:34:37 GMT

←Older revision		Revision as of 09:34, 5 May 2021
Line 3:		Line 3:
	<StructureSection load='1nev' size='340' side='right'caption='[[1nev]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1nev' size='340' side='right'caption='[[1nev]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1NEV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NEV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [~~http~~://pdbe.org/1nev PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1nev PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1nev ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [https://pdbe.org/1nev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [https://www.ebi.ac.uk/pdbsum/1nev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nev ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:36, 31 July 2019

OCA — Wed, 31 Jul 2019 07:36:52 GMT

←Older revision		Revision as of 07:36, 31 July 2019
Line 1:		Line 1:

	==A-tract decamer==		==A-tract decamer==
-	<StructureSection load='1nev' size='340' side='right' caption='[[1nev]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='1nev' size='340' side='right'caption='[[1nev]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEV FirstGlance]. <br>
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Barbic, A]]		[[Category: Barbic, A]]
	[[Category: Crothers, D M]]		[[Category: Crothers, D M]]

OCA at 10:24, 25 October 2017

OCA — Wed, 25 Oct 2017 10:24:26 GMT

←Older revision		Revision as of 10:24, 25 October 2017
Line 1:		Line 1:
		+
	==A-tract decamer==		==A-tract decamer==
	<StructureSection load='1nev' size='340' side='right' caption='[[1nev]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1nev' size='340' side='right' caption='[[1nev]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [http://pdbe.org/1nev PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [http://www.ebi.ac.uk/pdbsum/1nev PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [http://pdbe.org/1nev PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [http://www.ebi.ac.uk/pdbsum/1nev PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nev ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:33, 11 September 2015

OCA — Fri, 11 Sep 2015 02:33:39 GMT

←Older revision		Revision as of 02:33, 11 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [http://www.ebi.ac.uk/pdbsum/1nev PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [http://pdbe.org/1nev PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [http://www.ebi.ac.uk/pdbsum/1nev PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 13:		Line 13:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1nev" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:19, 22 December 2014

OCA — Mon, 22 Dec 2014 06:19:21 GMT

←Older revision		Revision as of 06:19, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [http://www.ebi.ac.uk/pdbsum/1nev PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [http://www.ebi.ac.uk/pdbsum/1nev PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Barbic, A.]]	+	[[Category: Barbic, A]]
-	[[Category: Crothers, D M.]]	+	[[Category: Crothers, D M]]
-	[[Category: Zimmer, D P.]]	+	[[Category: Zimmer, D P]]
	[[Category: A-tract]]		[[Category: A-tract]]
	[[Category: Dna]]		[[Category: Dna]]
	[[Category: Dna bending]]		[[Category: Dna bending]]
	[[Category: Dna decamer]]		[[Category: Dna decamer]]

OCA at 06:59, 9 June 2014

OCA — Mon, 09 Jun 2014 06:59:06 GMT

←Older revision		Revision as of 06:59, 9 June 2014
Line 1:		Line 1:
-	[[~~Image~~:1nev.~~png\|left\|200px~~]]	+	==A-tract decamer==
		+	<StructureSection load='1nev' size='340' side='right' caption='[[1nev]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NEV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nev RCSB], [http://www.ebi.ac.uk/pdbsum/1nev PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DNA sequences containing short adenine tracts are intrinsically curved and play a role in transcriptional regulation. Despite many high-resolution NMR and x-ray studies, the origins of curvature remain disputed. Long-range restraints provided by 85 residual dipolar couplings were measured for a DNA decamer containing an adenine (A)(4)-tract and used to refine the structure. The overall bend in the molecule is a result of in-phase negative roll in the A-tract and positive roll at its 5' junction, as well as positive and negative tilt inside the A-tract and near its junctions. The bend magnitude and direction obtained from NMR structures is 9.0 degrees into the minor groove in a coordinate frame located at the third AT base pair. We evaluated long-range and wedge models for DNA curvature and concluded that our data for A-tract curvature are best explained by a "delocalized bend" model. The global bend magnitude and direction of the NMR structure are in excellent agreement with the junction model parameters used to rationalize gel electrophoretic data and with preliminary results of a cyclization kinetics assay from our laboratory.

-	~~{{STRUCTURE_1nev\| PDB=1nev \| SCENE= }}~~	+	Structural origins of adenine-tract bending.,Barbic A, Zimmer DP, Crothers DM Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2369-73. Epub 2003 Feb 13. PMID:12586860<ref>PMID:12586860</ref>

-	~~===A-tract decamer===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12586860}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1nev]] is~~ a ~~2 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=1NEV OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012586860<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Barbic, A.]]		[[Category: Barbic, A.]]
	[[Category: Crothers, D M.]]		[[Category: Crothers, D M.]]

OCA: Protected "1nev" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 18:16:35 GMT

Protected "1nev" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:16, 14 August 2012

OCA at 18:16, 14 August 2012

OCA — Tue, 14 Aug 2012 18:16:34 GMT

←Older revision		Revision as of 18:16, 14 August 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1nev.png\|left\|200px]]		[[Image:1nev.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1nev", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1nev\| PDB=1nev \| SCENE= }}		{{STRUCTURE_1nev\| PDB=1nev \| SCENE= }}

	===A-tract decamer===		===A-tract decamer===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12586860}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12586860 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12586860}}		{{ABSTRACT_PUBMED_12586860}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA].	+	[[1nev]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA].

	==Reference==		==Reference==
-	~~Structural origins of adenine-tract bending., Barbic A, Zimmer DP, Crothers DM, Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2369-73. Epub 2003 Feb 13.~~ PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~12586860 12586860]~~	+	<ref group="xtra">PMID:012586860</ref><references group="xtra"/>
	[[Category: Barbic, A.]]		[[Category: Barbic, A.]]
	[[Category: Crothers, D M.]]		[[Category: Crothers, D M.]]
	[[Category: Zimmer, D P.]]		[[Category: Zimmer, D P.]]
	[[Category: A-tract]]		[[Category: A-tract]]
		+	[[Category: Dna]]
	[[Category: Dna bending]]		[[Category: Dna bending]]
	[[Category: Dna decamer]]		[[Category: Dna decamer]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:41:06 2008''

OCA at 14:41, 28 July 2008

OCA — Mon, 28 Jul 2008 14:41:10 GMT

←Older revision		Revision as of 14:41, 28 July 2008
Line 1:		Line 1:
-	[[Image:1nev.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1nev.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1nev\| PDB=1nev \| SCENE= }}		{{STRUCTURE_1nev\| PDB=1nev \| SCENE= }}

-	~~'''~~A-tract decamer~~'''~~	+	===A-tract decamer===


-	~~==Overview==~~	+	<!--
-	~~DNA sequences containing short adenine tracts are intrinsically curved and play a role in transcriptional regulation. Despite many high~~-~~resolution NMR and x~~-~~ray studies~~, the ~~origins of curvature remain disputed. Long-range restraints provided by 85 residual dipolar couplings were measured for a DNA decamer containing an adenine (A)(4)-tract and used~~ to ~~refine~~ the ~~structure~~. ~~The overall bend in the molecule is a result of in-phase negative roll in the A-tract and positive roll at its 5' junction~~, ~~as well as positive and negative tilt inside the A-tract and near its junctions. The bend magnitude and direction obtained from NMR structures~~ is ~~9.0 degrees into~~ the ~~minor groove in a coordinate frame located at the third AT base pair~~. ~~We evaluated long~~-~~range and wedge models for DNA curvature and concluded that our data for A~~-tract curvature are best explained by a "delocalized bend" model. The global bend magnitude and direction of the NMR structure are in excellent agreement with the junction model parameters used to rationalize gel electrophoretic data and with preliminary results of a cyclization kinetics assay from our laboratory.	+	The line below this paragraph, {{ABSTRACT_PUBMED_12586860}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 12586860 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_12586860}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA].

	==Reference==		==Reference==
Line 26:		Line 30:
	[[Category: Dna bending]]		[[Category: Dna bending]]
	[[Category: Dna decamer]]		[[Category: Dna decamer]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 02~~:26:54 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:41:06 2008''