1nf2 - Revision history

OCA at 08:49, 10 April 2024

OCA — Wed, 10 Apr 2024 08:49:10 GMT

←Older revision		Revision as of 08:49, 10 April 2024
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	<StructureSection load='1nf2' size='340' side='right'caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1nf2' size='340' side='right'caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_43589 Atcc 43589~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [https://pdbe.org/1nf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [https://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nf2 ProSAT], [https://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [https://pdbe.org/1nf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [https://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nf2 ProSAT], [https://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9WZB9_THEMA Q9WZB9_THEMA]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nf2 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nf2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We have determined the crystal structure of a phosphatase with a unique substrate binding domain from Thermotoga maritima, TM0651 (gi 4981173), at 2.2 A resolution by selenomethionine single-wavelength anomalous diffraction (SAD) techniques. TM0651 is a member of the haloacid dehalogenase (HAD) superfamily, with sequence homology to trehalose-6-phosphate phosphatase and sucrose-6(F)-phosphate phosphohydrolase. Selenomethionine labeled TM0651 crystallized in space group C2 with three monomers per asymmetric unit. Each monomer has approximate dimensions of 65 x 40 x 35 A(3), and contains two domains: a domain of known hydrolase fold characteristic of the HAD family, and a domain with a new tertiary fold consisting of a six-stranded beta-sheet surrounded by four alpha-helices. There is one disulfide bond between residues Cys35 and Cys265 in each monomer. One magnesium ion and one sulfate ion are bound in the active site. The superposition of active site residues with other HAD family members indicates that TM0651 is very likely a phosphatase that acts through the formation of a phosphoaspartate intermediate, which is supported by both NMR titration data and a biochemical assay. Structural and functional database searches and the presence of many aromatic residues in the interface of the two domains suggest the substrate of TM0651 is a carbohydrate molecule. From the crystal structure and NMR data, the protein likely undergoes a conformational change upon substrate binding.
-
-	Crystal structure of a phosphatase with a unique substrate binding domain from Thermotoga maritima.,Shin DH, Roberts A, Jancarik J, Yokota H, Kim R, Wemmer DE, Kim SH Protein Sci. 2003 Jul;12(7):1464-72. PMID:12824492<ref>PMID:12824492</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1nf2" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 43589]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Structural genomic]]
-	[[Category: Shin, D H]]
-	[[Category: Bsgc structure funded by nih]]
-	[[Category: Had family]]
-	[[Category: New fold]]
-	[[Category: Phosphatase]]
-	[[Category: PSI, Protein structure initiative]]
-	[[Category: Structural proteomic]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
		+	[[Category: Shin DH]]

OCA at 09:34, 5 May 2021

OCA — Wed, 05 May 2021 09:34:41 GMT

←Older revision		Revision as of 09:34, 5 May 2021
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	<StructureSection load='1nf2' size='340' side='right'caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1nf2' size='340' side='right'caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [~~http~~://pdbe.org/1nf2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1nf2 ProSAT], [~~http~~://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [https://pdbe.org/1nf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [https://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nf2 ProSAT], [https://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:17, 31 July 2019

OCA — Wed, 31 Jul 2019 07:17:56 GMT

←Older revision		Revision as of 07:17, 31 July 2019
Line 1:		Line 1:

	==X-ray crystal structure of TM0651 from Thermotoga maritima==		==X-ray crystal structure of TM0651 from Thermotoga maritima==
-	<StructureSection load='1nf2' size='340' side='right' caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1nf2' size='340' side='right'caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nf/1nf2_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nf/1nf2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 43589]]		[[Category: Atcc 43589]]
		+	[[Category: Large Structures]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Shin, D H]]		[[Category: Shin, D H]]

OCA at 10:08, 25 October 2017

OCA — Wed, 25 Oct 2017 10:08:04 GMT

←Older revision		Revision as of 10:08, 25 October 2017
Line 1:		Line 1:
		+
	==X-ray crystal structure of TM0651 from Thermotoga maritima==		==X-ray crystal structure of TM0651 from Thermotoga maritima==
	<StructureSection load='1nf2' size='340' side='right' caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1nf2' size='340' side='right' caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [http://pdbe.org/1nf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [http://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [http://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [http://pdbe.org/1nf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [http://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nf2 ProSAT], [http://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nf2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:27, 11 September 2015

OCA — Fri, 11 Sep 2015 10:27:01 GMT

←Older revision		Revision as of 10:27, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1nf2' size='340' side='right' caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1nf2' size='340' side='right' caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermotoga_maritima Thermotoga maritima~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [http://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [http://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [http://pdbe.org/1nf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [http://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [http://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1nf2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermotoga maritima~~]]	+	[[Category: Atcc 43589]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Shin, D H]]		[[Category: Shin, D H]]
Line 37:		Line 38:
	[[Category: PSI, Protein structure initiative]]		[[Category: PSI, Protein structure initiative]]
	[[Category: Structural proteomic]]		[[Category: Structural proteomic]]
		+	[[Category: Thermotoga maritima]]

OCA at 15:20, 5 January 2015

OCA — Mon, 05 Jan 2015 15:20:57 GMT

←Older revision		Revision as of 15:20, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [http://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [http://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [http://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [http://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
-	[[Category: ~~BSGC, Berkeley~~ Structural ~~Genomics Center.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Shin, D H~~.]]~~	+	[[Category: Shin, D H]]
-	~~[[Category: Berkeley structural genomics center~~]]	+
	[[Category: Bsgc structure funded by nih]]		[[Category: Bsgc structure funded by nih]]
	[[Category: Had family]]		[[Category: Had family]]
	[[Category: New fold]]		[[Category: New fold]]
	[[Category: Phosphatase]]		[[Category: Phosphatase]]
-	[[Category: Protein structure initiative]]	+	[[Category: PSI, Protein structure initiative]]
-	~~[[Category: Psi]]~~	+
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: Structural genomics-unknown function complex~~]]	+
	[[Category: Structural proteomic]]		[[Category: Structural proteomic]]
-	[[Category: Thermotoga maritima]]

OCA at 13:05, 28 September 2014

OCA — Sun, 28 Sep 2014 13:05:10 GMT

←Older revision		Revision as of 13:05, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1nf2.~~png\|left~~\|200px]]	+	==X-ray crystal structure of TM0651 from Thermotoga maritima==
		+	<StructureSection load='1nf2' size='340' side='right' caption='[[1nf2]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1nf2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NF2 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nf2 RCSB], [http://www.ebi.ac.uk/pdbsum/1nf2 PDBsum], [http://www.topsan.org/Proteins/BSGC/1nf2 TOPSAN]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nf/1nf2_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have determined the crystal structure of a phosphatase with a unique substrate binding domain from Thermotoga maritima, TM0651 (gi 4981173), at 2.2 A resolution by selenomethionine single-wavelength anomalous diffraction (SAD) techniques. TM0651 is a member of the haloacid dehalogenase (HAD) superfamily, with sequence homology to trehalose-6-phosphate phosphatase and sucrose-6(F)-phosphate phosphohydrolase. Selenomethionine labeled TM0651 crystallized in space group C2 with three monomers per asymmetric unit. Each monomer has approximate dimensions of 65 x 40 x 35 A(3), and contains two domains: a domain of known hydrolase fold characteristic of the HAD family, and a domain with a new tertiary fold consisting of a six-stranded beta-sheet surrounded by four alpha-helices. There is one disulfide bond between residues Cys35 and Cys265 in each monomer. One magnesium ion and one sulfate ion are bound in the active site. The superposition of active site residues with other HAD family members indicates that TM0651 is very likely a phosphatase that acts through the formation of a phosphoaspartate intermediate, which is supported by both NMR titration data and a biochemical assay. Structural and functional database searches and the presence of many aromatic residues in the interface of the two domains suggest the substrate of TM0651 is a carbohydrate molecule. From the crystal structure and NMR data, the protein likely undergoes a conformational change upon substrate binding.

-	~~{{STRUCTURE_1nf2\| PDB=1nf2 \| SCENE= }}~~	+	Crystal structure of a phosphatase with a unique substrate binding domain from Thermotoga maritima.,Shin DH, Roberts A, Jancarik J, Yokota H, Kim R, Wemmer DE, Kim SH Protein Sci. 2003 Jul;12(7):1464-72. PMID:12824492<ref>PMID:12824492</ref>

-	~~===X-ray crystal structure~~ of ~~TM0651 from Thermotoga maritima===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12824492}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1nf2]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermotoga_maritima Thermotoga maritima]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012824492<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: BSGC, Berkeley Structural Genomics Center.]]		[[Category: BSGC, Berkeley Structural Genomics Center.]]

OCA: Protected "1nf2" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 19:36:24 GMT

Protected "1nf2" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:36, 5 December 2012

OCA at 19:36, 5 December 2012

OCA — Wed, 05 Dec 2012 19:36:22 GMT

←Older revision		Revision as of 19:36, 5 December 2012
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-	{{Seed}}
	[[Image:1nf2.png\|left\|200px]]		[[Image:1nf2.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1nf2", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1nf2\| PDB=1nf2 \| SCENE= }}		{{STRUCTURE_1nf2\| PDB=1nf2 \| SCENE= }}

	===X-ray crystal structure of TM0651 from Thermotoga maritima===		===X-ray crystal structure of TM0651 from Thermotoga maritima===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12824492}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12824492 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_12824492}}		{{ABSTRACT_PUBMED_12824492}}

	==About this Structure==		==About this Structure==
-	~~1NF2~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA].	+	[[1nf2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF2 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12824492~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012824492</ref><references group="xtra"/>
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: BSGC, Berkeley Structural Genomics Center.]]		[[Category: BSGC, Berkeley Structural Genomics Center.]]
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	[[Category: Psi]]		[[Category: Psi]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
		+	[[Category: Structural genomics-unknown function complex]]
	[[Category: Structural proteomic]]		[[Category: Structural proteomic]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:52:40 2009''

OCA at 17:52, 17 February 2009

OCA — Tue, 17 Feb 2009 17:52:40 GMT

←Older revision		Revision as of 17:52, 17 February 2009
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	==Reference==		==Reference==
-	Crystal structure of a phosphatase with a unique substrate binding domain from Thermotoga maritima., Shin DH, Roberts A, Jancarik J, Yokota H, Kim R, Wemmer DE, Kim SH, Protein Sci. 2003 Jul;12(7):1464-72. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/12824492 12824492]~~	+	<ref group="xtra">PMID:12824492</ref><references group="xtra"/>
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: BSGC, Berkeley Structural Genomics Center.]]		[[Category: BSGC, Berkeley Structural Genomics Center.]]
Line 38:		Line 38:
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Nov 16 09~~:45:~~54 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:52:40 2009''