1ngu - Revision history

OCA at 18:53, 29 November 2023

OCA — Wed, 29 Nov 2023 18:53:23 GMT

←Older revision		Revision as of 18:53, 29 November 2023
Line 1:		Line 1:

	==NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand==		==NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand==
-	<StructureSection load='1ngu' size='340' side='right'caption='[[1ngu~~]], [[NMR_Ensembles_of_Models \| 5 NMR models~~]]' scene=''>	+	<StructureSection load='1ngu' size='340' side='right'caption='[[1ngu]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NGU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NGU FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1ngo\|1ngo]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [https://pdbe.org/1ngu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [https://www.ebi.ac.uk/pdbsum/1ngu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [https://pdbe.org/1ngu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [https://www.ebi.ac.uk/pdbsum/1ngu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngu ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gupta, G]]	+	[[Category: Gupta G]]
-	[[Category: Michalczyk, R]]	+	[[Category: Michalczyk R]]
-	[[Category: Shiflett~~, P R~~]]	+	[[Category: Shiflett PR]]
-	[[Category: Silks~~, L A~~]]	+	[[Category: Silks LA]]
-	[[Category: Taylor-McCabe~~, K J]]~~	+	[[Category: Taylor-McCabe KJ]]
-	~~[[Category: B-form dna hairpin]]~~	+
-	~~[[Category: Dna~~]]	+

OCA at 09:35, 5 May 2021

OCA — Wed, 05 May 2021 09:35:03 GMT

←Older revision		Revision as of 09:35, 5 May 2021
Line 3:		Line 3:
	<StructureSection load='1ngu' size='340' side='right'caption='[[1ngu]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>		<StructureSection load='1ngu' size='340' side='right'caption='[[1ngu]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1NGU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NGU FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ngo\|1ngo]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ngo\|1ngo]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [~~http~~://pdbe.org/1ngu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ngu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [https://pdbe.org/1ngu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [https://www.ebi.ac.uk/pdbsum/1ngu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngu ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:35, 31 July 2019

OCA — Wed, 31 Jul 2019 07:35:35 GMT

←Older revision		Revision as of 07:35, 31 July 2019
Line 1:		Line 1:

	==NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand==		==NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand==
-	<StructureSection load='1ngu' size='340' side='right' caption='[[1ngu]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>	+	<StructureSection load='1ngu' size='340' side='right'caption='[[1ngu]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NGU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NGU FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gupta, G]]		[[Category: Gupta, G]]
	[[Category: Michalczyk, R]]		[[Category: Michalczyk, R]]

OCA at 10:23, 25 October 2017

OCA — Wed, 25 Oct 2017 10:23:14 GMT

←Older revision		Revision as of 10:23, 25 October 2017
Line 1:		Line 1:
		+
	==NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand==		==NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand==
	<StructureSection load='1ngu' size='340' side='right' caption='[[1ngu]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>		<StructureSection load='1ngu' size='340' side='right' caption='[[1ngu]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NGU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NGU FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ngo\|1ngo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ngo\|1ngo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [http://pdbe.org/1ngu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [http://www.ebi.ac.uk/pdbsum/1ngu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [http://pdbe.org/1ngu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [http://www.ebi.ac.uk/pdbsum/1ngu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngu ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:15, 11 September 2015

OCA — Fri, 11 Sep 2015 03:15:19 GMT

←Older revision		Revision as of 03:15, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NGU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NGU FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ngo\|1ngo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ngo\|1ngo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [http://www.ebi.ac.uk/pdbsum/1ngu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [http://pdbe.org/1ngu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [http://www.ebi.ac.uk/pdbsum/1ngu PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ngu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:43, 5 January 2015

OCA — Mon, 05 Jan 2015 15:43:43 GMT

←Older revision		Revision as of 15:43, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NGU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NGU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ngo\|1ngo]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ngo\|1ngo]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [http://www.ebi.ac.uk/pdbsum/1ngu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [http://www.ebi.ac.uk/pdbsum/1ngu PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Gupta, G.]]	+	[[Category: Gupta, G]]
-	[[Category: Michalczyk, R.]]	+	[[Category: Michalczyk, R]]
-	[[Category: Shiflett, P R.]]	+	[[Category: Shiflett, P R]]
-	[[Category: Silks, L A.]]	+	[[Category: Silks, L A]]
-	[[Category: Taylor-McCabe, K J.]]	+	[[Category: Taylor-McCabe, K J]]
	[[Category: B-form dna hairpin]]		[[Category: B-form dna hairpin]]
	[[Category: Dna]]		[[Category: Dna]]

OCA at 16:53, 28 September 2014

OCA — Sun, 28 Sep 2014 16:53:17 GMT

←Older revision		Revision as of 16:53, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ngu.~~png~~\|~~left~~\|~~200px~~]]	+	==NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand==
		+	<StructureSection load='1ngu' size='340' side='right' caption='[[1ngu]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NGU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ngo\|1ngo]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ngu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ngu RCSB], [http://www.ebi.ac.uk/pdbsum/1ngu PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Three proteins, namely, protective antigen (PA), edema factor (EF), and lethal factor (LF), encoded by the pX01 plasmid of Bacillus anthracis play a major role in the pathogenesis of target host cells. PA combines with EF and LF to form bipartite PA-EF and PA-LF toxins and facilitates intracellular delivery of EF and LF both of which cause cytotoxicity to the host. Since the level of PA is crucial to pathogenesis by anthrax toxins, it is important to understand how the host environment regulates the expression of the PA (or pagA) gene by utilizing the 5' and 3' untranslated regions (UTR). The 5' UTR sequence determines the initiation of transcription, whereas the 3' UTR sequence determines the efficient termination and stability of the transcript. Although, the role of the 5'UTR sequence of pagA has been investigated, little is known about the role of the 3' UTR. Since hairpin formation at the 3'UTR of a gene is an established mechanism for efficient termination and stability of the transcript, we carried out structural studies, including gel electrophoresis, circular dichroism, and two-dimensional nuclear magnetic resonance spectroscopy, to determine whether the 3' UTR sequences of pagA also form hairpin structures. Our results unequivocally demonstrate that both the coding and the noncoding 3' UTR sequences form stable hairpin structures. It is quite likely that the hairpins at the 3'UTR may contribute to efficient termination and stability of the pagA transcript.

-	~~{{STRUCTURE_1ngu\| PDB=1ngu \| SCENE= }}~~	+	Structural studies on the hairpins at the 3' untranslated region of an anthrax toxin gene.,Shiflett PR, Taylor-McCabe KJ, Michalczyk R, Silks LA, Gupta G Biochemistry. 2003 May 27;42(20):6078-89. PMID:12755609<ref>PMID:12755609</ref>

-	~~===NMR Structure~~ of ~~Putative 3'Terminator for B~~. ~~Anthracis pagA Gene Noncoding Strand===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12755609}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1ngu]] is a 1 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA].~~	+	</StructureSection>
	[[Category: Gupta, G.]]		[[Category: Gupta, G.]]
	[[Category: Michalczyk, R.]]		[[Category: Michalczyk, R.]]

OCA: Protected "1ngu" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 19:58:31 GMT

Protected "1ngu" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:58, 5 December 2012

OCA at 19:58, 5 December 2012

OCA — Wed, 05 Dec 2012 19:58:29 GMT

←Older revision		Revision as of 19:58, 5 December 2012
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-	{{Seed}}
	[[Image:1ngu.png\|left\|200px]]		[[Image:1ngu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ngu", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ngu\| PDB=1ngu \| SCENE= }}		{{STRUCTURE_1ngu\| PDB=1ngu \| SCENE= }}

	===NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand===		===NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12755609}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12755609 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12755609}}		{{ABSTRACT_PUBMED_12755609}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA].	+	[[1ngu]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA].
-		+
-	~~==Reference==~~	+
-	Structural studies on the hairpins at the 3' untranslated region of an anthrax toxin gene., Shiflett PR, Taylor-McCabe KJ, Michalczyk R, Silks LA, Gupta G, Biochemistry. 2003 May 27;42(20):6078-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12755609 12755609]	+
	[[Category: Gupta, G.]]		[[Category: Gupta, G.]]
	[[Category: Michalczyk, R.]]		[[Category: Michalczyk, R.]]
Line 30:		Line 15:
	[[Category: Taylor-McCabe, K J.]]		[[Category: Taylor-McCabe, K J.]]
	[[Category: B-form dna hairpin]]		[[Category: B-form dna hairpin]]
-		+	[[Category: Dna]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Jul 28 18:56:52 2008''~~	+

OCA at 15:56, 28 July 2008

OCA — Mon, 28 Jul 2008 15:56:56 GMT

←Older revision		Revision as of 15:56, 28 July 2008
Line 1:		Line 1:
-	[[Image:1ngu.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1ngu.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1ngu\| PDB=1ngu \| SCENE= }}		{{STRUCTURE_1ngu\| PDB=1ngu \| SCENE= }}

-	~~'''~~NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand~~'''~~	+	===NMR Structure of Putative 3'Terminator for B. Anthracis pagA Gene Noncoding Strand===


-	~~==Overview==~~	+	<!--
-	~~Three proteins~~, ~~namely~~, ~~protective antigen (PA), edema factor (EF), and lethal factor (LF), encoded by~~ the pX01 plasmid of Bacillus anthracis play a major role in the pathogenesis of target host cells. PA combines with EF and LF to form bipartite PA-EF and PA-LF toxins and facilitates intracellular delivery of EF and LF both of which cause cytotoxicity to the ~~host. Since the level of PA is crucial to pathogenesis by anthrax toxins,~~ it ~~is important to understand how the host environment regulates the expression of the PA (or pagA) gene by utilizing the 5' and 3' untranslated regions (UTR)~~. ~~The 5' UTR sequence determines the initiation of transcription, whereas the 3' UTR sequence determines the efficient termination and stability of the transcript~~. ~~Although, the role of the 5'UTR sequence of pagA has been investigated~~, ~~little~~ is ~~known about~~ the ~~role of the 3' UTR~~. Since hairpin formation at the 3'UTR of a gene is an established mechanism for efficient termination and stability of the transcript, we carried out structural studies, including gel electrophoresis, circular dichroism, and two-dimensional nuclear magnetic resonance spectroscopy, to determine whether the 3' UTR sequences of pagA also form hairpin structures. Our results unequivocally demonstrate that both the coding and the noncoding 3' UTR sequences form stable hairpin structures. It is quite likely that the hairpins at the 3'UTR may contribute to efficient termination and stability of the pagA transcript.	+	The line below this paragraph, {{ABSTRACT_PUBMED_12755609}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 12755609 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_12755609}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGU OCA].

	==Reference==		==Reference==
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	[[Category: Taylor-McCabe, K J.]]		[[Category: Taylor-McCabe, K J.]]
	[[Category: B-form dna hairpin]]		[[Category: B-form dna hairpin]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 02~~:30:53 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:56:52 2008''