1nx1 - Revision history

OCA at 08:00, 14 February 2024

OCA — Wed, 14 Feb 2024 08:00:18 GMT

←Older revision		Revision as of 08:00, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1nx1' size='340' side='right'caption='[[1nx1]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1nx1' size='340' side='right'caption='[[1nx1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nx1]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pig Pig~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1NX1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nx1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NX1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[1nx0\|1nx0]], [[1nx2\|1nx2]], [[1nx3\|1nx3]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [https://pdbe.org/1nx1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [https://www.ebi.ac.uk/pdbsum/1nx1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nx1 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [~~http~~://pdbe.org/1nx1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1nx1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1nx1 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CPNS1_PIG CPNS1_PIG]] Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. [[http://www.uniprot.org/uniprot/ICAL_CHLAE ICAL_CHLAE]] Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.	+	[https://www.uniprot.org/uniprot/CPNS1_PIG CPNS1_PIG] Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nx1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nx1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Ca(2+)-dependent cysteine protease calpain along with its endogenous inhibitor calpastatin is widely distributed. The interactions between calpain and calpastatin have been studied to better understand the nature of calpain inhibition by calpastatin, which can aid the design of small molecule inhibitors to calpain. Here we present the crystal structure of a complex between a calpastatin peptide and the calcium-binding domain VI of calpain. DIC19 is a 19 residue peptide, which corresponds to one of the three interacting domains of calpastatin, which is known to interact with domain VI of calpain. We present two crystal structures of DIC19 bound to domain VI of calpain, determined by molecular replacement methods to 2.5A and 2.2A resolution. In the process of crystallizing the inhibitor complex, a new native crystal form was identified which had the homodimer 2-fold axis along a crystallographic axis as opposed to the previously observed dimer in the asymmetric unit. The crystal structures of the native domain VI and its inhibitor PD150606 (3-(4-iodophenyl)-2-mercapto-(Z)-2-propenoic acid) complex were determined with the help of molecular replacement methods to 2.0A and 2.3A resolution, respectively. In addition, we built a homology model for the complex between domain IV and DIA19 peptide of calpastatin. Finally, we present a model for the calpastatin-inhibited calpain.
-
-	A structural model for the inhibition of calpain by calpastatin: crystal structures of the native domain VI of calpain and its complexes with calpastatin peptide and a small molecule inhibitor.,Todd B, Moore D, Deivanayagam CC, Lin GD, Chattopadhyay D, Maki M, Wang KK, Narayana SV J Mol Biol. 2003 Apr 18;328(1):131-46. PMID:12684003<ref>PMID:12684003</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1nx1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Calpain 3D structures\|Calpain 3D structures]]		*[[Calpain 3D structures\|Calpain 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pig~~]]	+	[[Category: Sus scrofa]]
-	[[Category: Chattopadhyay, D]]	+	[[Category: Chattopadhyay D]]
-	[[Category: Deivanayagam~~, C C.S~~]]	+	[[Category: Deivanayagam CCS]]
-	[[Category: Lin, G D]]	+	[[Category: Lin G-D]]
-	[[Category: Maki, M]]	+	[[Category: Maki M]]
-	[[Category: Moore, D]]	+	[[Category: Moore D]]
-	[[Category: Narayana~~, S V.L~~]]	+	[[Category: Narayana SVL]]
-	[[Category: Todd, B]]	+	[[Category: Todd B]]
-	[[Category: Wang~~, K K.W]]~~	+	[[Category: Wang KKW]]
-	~~[[Category: Calcium binding]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex~~]]	+

OCA at 08:58, 2 December 2020

OCA — Wed, 02 Dec 2020 08:58:50 GMT

←Older revision		Revision as of 08:58, 2 December 2020
Line 1:		Line 1:

	==Calpain Domain VI Complexed with Calpastatin Inhibitory Domain C (DIC)==		==Calpain Domain VI Complexed with Calpastatin Inhibitory Domain C (DIC)==
-	<StructureSection load='1nx1' size='340' side='right' caption='[[1nx1]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1nx1' size='340' side='right'caption='[[1nx1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1NX1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1NX1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nx0\|1nx0]], [[1nx2\|1nx2]], [[1nx3\|1nx3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1nx0\|1nx0]], [[1nx2\|1nx2]], [[1nx3\|1nx3]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [http://pdbe.org/1nx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [http://www.ebi.ac.uk/pdbsum/1nx1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nx1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [http://pdbe.org/1nx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [http://www.ebi.ac.uk/pdbsum/1nx1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nx1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1nx1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1nx1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Calpain 3D structures\|Calpain 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pig]]		[[Category: Pig]]
	[[Category: Chattopadhyay, D]]		[[Category: Chattopadhyay, D]]

OCA at 07:37, 7 February 2018

OCA — Wed, 07 Feb 2018 07:37:39 GMT

←Older revision		Revision as of 07:37, 7 February 2018
Line 1:		Line 1:
		+
	==Calpain Domain VI Complexed with Calpastatin Inhibitory Domain C (DIC)==		==Calpain Domain VI Complexed with Calpastatin Inhibitory Domain C (DIC)==
	<StructureSection load='1nx1' size='340' side='right' caption='[[1nx1]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1nx1' size='340' side='right' caption='[[1nx1]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nx0\|1nx0]], [[1nx2\|1nx2]], [[1nx3\|1nx3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nx0\|1nx0]], [[1nx2\|1nx2]], [[1nx3\|1nx3]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [http://pdbe.org/1nx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [http://www.ebi.ac.uk/pdbsum/1nx1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [http://pdbe.org/1nx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [http://www.ebi.ac.uk/pdbsum/1nx1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nx1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nx/1nx1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nx/1nx1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1nx1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1nx1" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Calpain\|Calpain]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:18, 9 February 2016

OCA — Tue, 09 Feb 2016 00:18:31 GMT

←Older revision		Revision as of 00:18, 9 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nx1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:45, 10 September 2015

OCA — Thu, 10 Sep 2015 16:45:49 GMT

←Older revision		Revision as of 16:45, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1nx1' size='340' side='right' caption='[[1nx1]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1nx1' size='340' side='right' caption='[[1nx1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sus_scrofa Sus scrofa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NX1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NX1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nx0\|1nx0]], [[1nx2\|1nx2]], [[1nx3\|1nx3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nx0\|1nx0]], [[1nx2\|1nx2]], [[1nx3\|1nx3]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 ~~Sus scrofa~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [http://www.ebi.ac.uk/pdbsum/1nx1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [http://pdbe.org/1nx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [http://www.ebi.ac.uk/pdbsum/1nx1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1nx1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sus scrofa~~]]	+	[[Category: Pig]]
	[[Category: Chattopadhyay, D]]		[[Category: Chattopadhyay, D]]
	[[Category: Deivanayagam, C C.S]]		[[Category: Deivanayagam, C C.S]]

OCA at 03:09, 25 December 2014

OCA — Thu, 25 Dec 2014 03:09:11 GMT

←Older revision		Revision as of 03:09, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NX1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NX1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nx0\|1nx0]], [[1nx2\|1nx2]], [[1nx3\|1nx3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nx0\|1nx0]], [[1nx2\|1nx2]], [[1nx3\|1nx3]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 Sus scrofa])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 Sus scrofa])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [http://www.ebi.ac.uk/pdbsum/1nx1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [http://www.ebi.ac.uk/pdbsum/1nx1 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CPNS1_PIG CPNS1_PIG]] Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. [[http://www.uniprot.org/uniprot/ICAL_CHLAE ICAL_CHLAE]] Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Chattopadhyay, D.]]	+	[[Category: Chattopadhyay, D]]
-	[[Category: Deivanayagam, C C.S.]]	+	[[Category: Deivanayagam, C C.S]]
-	[[Category: Lin, G D.]]	+	[[Category: Lin, G D]]
-	[[Category: Maki, M.]]	+	[[Category: Maki, M]]
-	[[Category: Moore, D.]]	+	[[Category: Moore, D]]
-	[[Category: Narayana, S V.L.]]	+	[[Category: Narayana, S V.L]]
-	[[Category: Todd, B.]]	+	[[Category: Todd, B]]
-	[[Category: Wang, K K.W.]]	+	[[Category: Wang, K K.W]]
	[[Category: Calcium binding]]		[[Category: Calcium binding]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]

OCA at 13:33, 28 September 2014

OCA — Sun, 28 Sep 2014 13:33:37 GMT

←Older revision		Revision as of 13:33, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1nx1.~~png~~\|~~left~~\|200px]]	+	==Calpain Domain VI Complexed with Calpastatin Inhibitory Domain C (DIC)==
		+	<StructureSection load='1nx1' size='340' side='right' caption='[[1nx1]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1nx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NX1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nx0\|1nx0]], [[1nx2\|1nx2]], [[1nx3\|1nx3]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 Sus scrofa])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nx1 RCSB], [http://www.ebi.ac.uk/pdbsum/1nx1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nx/1nx1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Ca(2+)-dependent cysteine protease calpain along with its endogenous inhibitor calpastatin is widely distributed. The interactions between calpain and calpastatin have been studied to better understand the nature of calpain inhibition by calpastatin, which can aid the design of small molecule inhibitors to calpain. Here we present the crystal structure of a complex between a calpastatin peptide and the calcium-binding domain VI of calpain. DIC19 is a 19 residue peptide, which corresponds to one of the three interacting domains of calpastatin, which is known to interact with domain VI of calpain. We present two crystal structures of DIC19 bound to domain VI of calpain, determined by molecular replacement methods to 2.5A and 2.2A resolution. In the process of crystallizing the inhibitor complex, a new native crystal form was identified which had the homodimer 2-fold axis along a crystallographic axis as opposed to the previously observed dimer in the asymmetric unit. The crystal structures of the native domain VI and its inhibitor PD150606 (3-(4-iodophenyl)-2-mercapto-(Z)-2-propenoic acid) complex were determined with the help of molecular replacement methods to 2.0A and 2.3A resolution, respectively. In addition, we built a homology model for the complex between domain IV and DIA19 peptide of calpastatin. Finally, we present a model for the calpastatin-inhibited calpain.

-	~~{{STRUCTURE_1nx1\| PDB=1nx1 \| SCENE= }}~~	+	A structural model for the inhibition of calpain by calpastatin: crystal structures of the native domain VI of calpain and its complexes with calpastatin peptide and a small molecule inhibitor.,Todd B, Moore D, Deivanayagam CC, Lin GD, Chattopadhyay D, Maki M, Wang KK, Narayana SV J Mol Biol. 2003 Apr 18;328(1):131-46. PMID:12684003<ref>PMID:12684003</ref>

-	~~===Calpain Domain VI Complexed with Calpastatin Inhibitory Domain C (DIC)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12684003}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1nx1]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sus_scrofa Sus scrofa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA].~~	+

	==See Also==		==See Also==
	*[[Calpain\|Calpain]]		*[[Calpain\|Calpain]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012684003<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Chattopadhyay, D.]]		[[Category: Chattopadhyay, D.]]

OCA at 11:58, 20 October 2012

OCA — Sat, 20 Oct 2012 11:58:25 GMT

←Older revision		Revision as of 11:58, 20 October 2012
Line 1:		Line 1:
	[[Image:1nx1.png\|left\|200px]]		[[Image:1nx1.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1nx1", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1nx1\| PDB=1nx1 \| SCENE= }}		{{STRUCTURE_1nx1\| PDB=1nx1 \| SCENE= }}

	===Calpain Domain VI Complexed with Calpastatin Inhibitory Domain C (DIC)===		===Calpain Domain VI Complexed with Calpastatin Inhibitory Domain C (DIC)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12684003}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12684003 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12684003}}		{{ABSTRACT_PUBMED_12684003}}

	==About this Structure==		==About this Structure==
	[[1nx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA].		[[1nx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA].
		+
		+	==See Also==
		+	*[[Calpain\|Calpain]]

	==Reference==		==Reference==

OCA: Protected "1nx1" [edit=sysop:move=sysop]

OCA — Wed, 02 May 2012 18:33:54 GMT

Protected "1nx1" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:33, 2 May 2012

OCA at 18:33, 2 May 2012

OCA — Wed, 02 May 2012 18:33:53 GMT

←Older revision		Revision as of 18:33, 2 May 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1nx1.png\|left\|200px]]		[[Image:1nx1.png\|left\|200px]]

Line 20:		Line 19:

	==About this Structure==		==About this Structure==
-	~~1NX1~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA].	+	[[1nx1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX1 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12684003~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012684003</ref><references group="xtra"/>
-	~~[[Category: Hydrolase]]~~	+
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Chattopadhyay, D.]]		[[Category: Chattopadhyay, D.]]
Line 35:		Line 33:
	[[Category: Wang, K K.W.]]		[[Category: Wang, K K.W.]]
	[[Category: Calcium binding]]		[[Category: Calcium binding]]
-	[[Category: Hydrolase]]	+	[[Category: Hydrolase-hydrolase inhibitor complex]]
-		+
-	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 04:49:16 2009''~~	+