1o8t - Revision history

OCA at 05:31, 15 May 2024

OCA — Wed, 15 May 2024 05:31:38 GMT

←Older revision		Revision as of 05:31, 15 May 2024
Line 21:		Line 21:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o8t ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o8t ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Apolipoprotein CII (apoCII), a surface constituent of plasma lipoproteins, is the activator for lipoprotein lipase (LPL) and is therefore central for lipid transport in blood. The three-dimensional structure of (13)C-, (15)N-enriched human full-length apoCII in complex with sodium dodecyl sulfate (SDS) micelles is reported. In addition to the structure determination, (15)N-relaxation measurements have been performed at two magnetic fields to characterize the dynamics of the backbone of apoCII in the complex. The relaxation data also provided global structural constraints, viz. the orientation of helices in the complex. In addition, global constraints were derived from the fact that apoCII helices are attached to the surface of the SDS micelle and that the hydrophobic moments of each helix faces the interior of the micelle. These three categories of global constraints, together with the local classical NMR constraints, were sufficient to define the 3D structure of the apoCII-SDS micelle complex. To our knowledge, this presents the first example in which the global structure of a protein-SDS micelle complex has been determined. The C-terminal helix of apoCII is known to be responsible for the activation of LPL. This helix is distinguished from the other helices by a higher degree of internal motion on the nanosecond time scale as shown by the relaxation data. The overall structure and the internal dynamics, combined with previous mutation data, give important clues toward a possible mechanism for the activation of LPL by apoCII.
		+
		+	Global structure and dynamics of human apolipoprotein CII in complex with micelles: evidence for increased mobility of the helix involved in the activation of lipoprotein lipase.,Zdunek J, Martinez GV, Schleucher J, Lycksell PO, Yin Y, Nilsson S, Shen Y, Olivecrona G, Wijmenga S Biochemistry. 2003 Feb 25;42(7):1872-89. PMID:12590574<ref>PMID:12590574</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1o8t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:55, 10 April 2024

OCA — Wed, 10 Apr 2024 08:55:58 GMT

←Older revision		Revision as of 08:55, 10 April 2024
Line 1:		Line 1:

	==Global Structure and Dynamics of Human Apolipoprotein CII in Complex with Micelles: Evidence for increased mobility of the helix involved in the activation of lipoprotein lipase==		==Global Structure and Dynamics of Human Apolipoprotein CII in Complex with Micelles: Evidence for increased mobility of the helix involved in the activation of lipoprotein lipase==
-	<StructureSection load='1o8t' size='340' side='right'caption='[[1o8t~~]], [[NMR_Ensembles_of_Models \| 68 NMR models~~]]' scene=''>	+	<StructureSection load='1o8t' size='340' side='right'caption='[[1o8t]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O8T FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1by6\|1by6]], [[1i5j\|1i5j]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [https://pdbe.org/1o8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [https://www.ebi.ac.uk/pdbsum/1o8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o8t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [https://pdbe.org/1o8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [https://www.ebi.ac.uk/pdbsum/1o8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o8t ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/APOC2_HUMAN APOC2_HUMAN] Defects in APOC2 are the cause of hyperlipoproteinemia type 1B (HLPP1B) [MIM:[https://omim.org/entry/207750 207750]. It is an autosomal recessive trait characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis.<ref>PMID:8323539</ref>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~APC2_HUMAN APC2_HUMAN~~]~~] Promotes rapid degradation~~ of ~~CTNNB1~~ and ~~may function as~~ a ~~tumor suppressor. May~~ function ~~in Wnt signaling~~.~~<ref>PMID:9823329</ref> <ref>PMID:10021369</ref> <ref>PMID:11691822</ref>~~	+	[https://www.uniprot.org/uniprot/APOC2_HUMAN APOC2_HUMAN] Component of the very low density lipoprotein (VLDL) fraction in plasma, and is an activator of several triacylglycerol lipases. The association of APOC2 with plasma chylomicrons, VLDL, and HDL is reversible, a function of the secretion and catabolism of triglyceride-rich lipoproteins, and changes rapidly.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 21:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o8t ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o8t ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Apolipoprotein CII (apoCII), a surface constituent of plasma lipoproteins, is the activator for lipoprotein lipase (LPL) and is therefore central for lipid transport in blood. The three-dimensional structure of (13)C-, (15)N-enriched human full-length apoCII in complex with sodium dodecyl sulfate (SDS) micelles is reported. In addition to the structure determination, (15)N-relaxation measurements have been performed at two magnetic fields to characterize the dynamics of the backbone of apoCII in the complex. The relaxation data also provided global structural constraints, viz. the orientation of helices in the complex. In addition, global constraints were derived from the fact that apoCII helices are attached to the surface of the SDS micelle and that the hydrophobic moments of each helix faces the interior of the micelle. These three categories of global constraints, together with the local classical NMR constraints, were sufficient to define the 3D structure of the apoCII-SDS micelle complex. To our knowledge, this presents the first example in which the global structure of a protein-SDS micelle complex has been determined. The C-terminal helix of apoCII is known to be responsible for the activation of LPL. This helix is distinguished from the other helices by a higher degree of internal motion on the nanosecond time scale as shown by the relaxation data. The overall structure and the internal dynamics, combined with previous mutation data, give important clues toward a possible mechanism for the activation of LPL by apoCII.
-
-	Global structure and dynamics of human apolipoprotein CII in complex with micelles: evidence for increased mobility of the helix involved in the activation of lipoprotein lipase.,Zdunek J, Martinez GV, Schleucher J, Lycksell PO, Yin Y, Nilsson S, Shen Y, Olivecrona G, Wijmenga S Biochemistry. 2003 Feb 25;42(7):1872-89. PMID:12590574<ref>PMID:12590574</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1o8t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lycksell~~, P O~~]]	+	[[Category: Lycksell PO]]
-	[[Category: Martinez~~, G V~~]]	+	[[Category: Martinez GV]]
-	[[Category: Nilsson, S]]	+	[[Category: Nilsson S]]
-	[[Category: Olivecrona, G]]	+	[[Category: Olivecrona G]]
-	[[Category: Schleucher, J]]	+	[[Category: Schleucher J]]
-	[[Category: Shen, Y]]	+	[[Category: Shen Y]]
-	[[Category: Wijmenga, S]]	+	[[Category: Wijmenga S]]
-	[[Category: Yin, Y]]	+	[[Category: Yin Y]]
-	[[Category: Zdunek, J]]	+	[[Category: Zdunek J]]
-	~~[[Category: Activation mechanism]]~~	+
-	~~[[Category: Apocii]]~~	+
-	~~[[Category: Chylomicron]]~~	+
-	~~[[Category: Domain motion]]~~	+
-	~~[[Category: Dynamic]]~~	+
-	~~[[Category: Global structure]]~~	+
-	~~[[Category: Helix]]~~	+
-	~~[[Category: Lipid degradation]]~~	+
-	~~[[Category: Lipid transport]]~~	+
-	~~[[Category: Local structure]]~~	+
-	~~[[Category: Lpl]]~~	+
-	~~[[Category: Micelle]]~~	+
-	~~[[Category: Sd~~]]	+

OCA at 07:09, 25 August 2021

OCA — Wed, 25 Aug 2021 07:09:59 GMT

←Older revision		Revision as of 07:09, 25 August 2021
Line 3:		Line 3:
	<StructureSection load='1o8t' size='340' side='right'caption='[[1o8t]], [[NMR_Ensembles_of_Models \| 68 NMR models]]' scene=''>		<StructureSection load='1o8t' size='340' side='right'caption='[[1o8t]], [[NMR_Ensembles_of_Models \| 68 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1O8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O8T FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1by6\|1by6]], [[1i5j\|1i5j]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1by6\|1by6]], [[1i5j\|1i5j]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [~~http~~://pdbe.org/1o8t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1o8t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1o8t ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [https://pdbe.org/1o8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [https://www.ebi.ac.uk/pdbsum/1o8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o8t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/APC2_HUMAN APC2_HUMAN]] Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling.<ref>PMID:9823329</ref> <ref>PMID:10021369</ref> <ref>PMID:11691822</ref>	+	[[https://www.uniprot.org/uniprot/APC2_HUMAN APC2_HUMAN]] Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling.<ref>PMID:9823329</ref> <ref>PMID:10021369</ref> <ref>PMID:11691822</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:38, 20 November 2019

OCA — Wed, 20 Nov 2019 18:38:57 GMT

←Older revision		Revision as of 18:38, 20 November 2019
Line 1:		Line 1:

	==Global Structure and Dynamics of Human Apolipoprotein CII in Complex with Micelles: Evidence for increased mobility of the helix involved in the activation of lipoprotein lipase==		==Global Structure and Dynamics of Human Apolipoprotein CII in Complex with Micelles: Evidence for increased mobility of the helix involved in the activation of lipoprotein lipase==
-	<StructureSection load='1o8t' size='340' side='right' caption='[[1o8t]], [[NMR_Ensembles_of_Models \| 68 NMR models]]' scene=''>	+	<StructureSection load='1o8t' size='340' side='right'caption='[[1o8t]], [[NMR_Ensembles_of_Models \| 68 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O8T FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O8T FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Lycksell, P O]]		[[Category: Lycksell, P O]]
	[[Category: Martinez, G V]]		[[Category: Martinez, G V]]

OCA at 07:25, 7 February 2018

OCA — Wed, 07 Feb 2018 07:25:37 GMT

←Older revision		Revision as of 07:25, 7 February 2018
Line 1:		Line 1:
-	==~~GLOBAL STRUCTURE AND DYNAMICS OF HUMAN APOLIPOPROTEIN~~ CII ~~IN COMPLEX WITH MICELLES~~: ~~EVIDENCE FOR INCREASED MOBILITY OF THE HELIX INVOLVVED IN THE ACTIVATION OF LIPOPROTEIN LIPASE.~~==	+
		+	==Global Structure and Dynamics of Human Apolipoprotein CII in Complex with Micelles: Evidence for increased mobility of the helix involved in the activation of lipoprotein lipase==
	<StructureSection load='1o8t' size='340' side='right' caption='[[1o8t]], [[NMR_Ensembles_of_Models \| 68 NMR models]]' scene=''>		<StructureSection load='1o8t' size='340' side='right' caption='[[1o8t]], [[NMR_Ensembles_of_Models \| 68 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O8T FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O8T FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1by6\|1by6]], [[1i5j\|1i5j]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1by6\|1by6]], [[1i5j\|1i5j]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [http://pdbe.org/1o8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [http://www.ebi.ac.uk/pdbsum/1o8t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [http://pdbe.org/1o8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [http://www.ebi.ac.uk/pdbsum/1o8t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1o8t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o8/1o8t_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o8/1o8t_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:53, 9 February 2016

OCA — Tue, 09 Feb 2016 20:53:35 GMT

←Older revision		Revision as of 20:53, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o8t ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:56, 11 September 2015

OCA — Fri, 11 Sep 2015 01:56:21 GMT

←Older revision		Revision as of 01:56, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1o8t' size='340' side='right' caption='[[1o8t]], [[NMR_Ensembles_of_Models \| 68 NMR models]]' scene=''>		<StructureSection load='1o8t' size='340' side='right' caption='[[1o8t]], [[NMR_Ensembles_of_Models \| 68 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O8T FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1by6\|1by6]], [[1i5j\|1i5j]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1by6\|1by6]], [[1i5j\|1i5j]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [http://www.ebi.ac.uk/pdbsum/1o8t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [http://pdbe.org/1o8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [http://www.ebi.ac.uk/pdbsum/1o8t PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1o8t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Lycksell, P O]]		[[Category: Lycksell, P O]]
	[[Category: Martinez, G V]]		[[Category: Martinez, G V]]

OCA at 09:27, 25 December 2014

OCA — Thu, 25 Dec 2014 09:27:53 GMT

←Older revision		Revision as of 09:27, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O8T FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O8T FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1by6\|1by6]], [[1i5j\|1i5j]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1by6\|1by6]], [[1i5j\|1i5j]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [http://www.ebi.ac.uk/pdbsum/1o8t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [http://www.ebi.ac.uk/pdbsum/1o8t PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/APC2_HUMAN APC2_HUMAN]] Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling.<ref>PMID:9823329</ref> <ref>PMID:10021369</ref> <ref>PMID:11691822</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Lycksell, P O.]]	+	[[Category: Lycksell, P O]]
-	[[Category: Martinez, G V.]]	+	[[Category: Martinez, G V]]
-	[[Category: Nilsson, S.]]	+	[[Category: Nilsson, S]]
-	[[Category: Olivecrona, G.]]	+	[[Category: Olivecrona, G]]
-	[[Category: Schleucher, J.]]	+	[[Category: Schleucher, J]]
-	[[Category: Shen, Y.]]	+	[[Category: Shen, Y]]
-	[[Category: Wijmenga, S.]]	+	[[Category: Wijmenga, S]]
-	[[Category: Yin, Y.]]	+	[[Category: Yin, Y]]
-	[[Category: Zdunek, J.]]	+	[[Category: Zdunek, J]]
	[[Category: Activation mechanism]]		[[Category: Activation mechanism]]
	[[Category: Apocii]]		[[Category: Apocii]]

OCA at 16:07, 29 September 2014

OCA — Mon, 29 Sep 2014 16:07:05 GMT

←Older revision		Revision as of 16:07, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1o8t\| PDB=1o8t \| SCENE= }}~~	+	==GLOBAL STRUCTURE AND DYNAMICS OF HUMAN APOLIPOPROTEIN CII IN COMPLEX WITH MICELLES: EVIDENCE FOR INCREASED MOBILITY OF THE HELIX INVOLVVED IN THE ACTIVATION OF LIPOPROTEIN LIPASE.==
-	===GLOBAL STRUCTURE AND DYNAMICS OF HUMAN APOLIPOPROTEIN CII IN COMPLEX WITH MICELLES: EVIDENCE FOR INCREASED MOBILITY OF THE HELIX INVOLVVED IN THE ACTIVATION OF LIPOPROTEIN LIPASE.===	+	<StructureSection load='1o8t' size='340' side='right' caption='[[1o8t]], [[NMR_Ensembles_of_Models \| 68 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_12590574}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1o8t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O8T FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1by6\|1by6]], [[1i5j\|1i5j]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o8t RCSB], [http://www.ebi.ac.uk/pdbsum/1o8t PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o8/1o8t_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Apolipoprotein CII (apoCII), a surface constituent of plasma lipoproteins, is the activator for lipoprotein lipase (LPL) and is therefore central for lipid transport in blood. The three-dimensional structure of (13)C-, (15)N-enriched human full-length apoCII in complex with sodium dodecyl sulfate (SDS) micelles is reported. In addition to the structure determination, (15)N-relaxation measurements have been performed at two magnetic fields to characterize the dynamics of the backbone of apoCII in the complex. The relaxation data also provided global structural constraints, viz. the orientation of helices in the complex. In addition, global constraints were derived from the fact that apoCII helices are attached to the surface of the SDS micelle and that the hydrophobic moments of each helix faces the interior of the micelle. These three categories of global constraints, together with the local classical NMR constraints, were sufficient to define the 3D structure of the apoCII-SDS micelle complex. To our knowledge, this presents the first example in which the global structure of a protein-SDS micelle complex has been determined. The C-terminal helix of apoCII is known to be responsible for the activation of LPL. This helix is distinguished from the other helices by a higher degree of internal motion on the nanosecond time scale as shown by the relaxation data. The overall structure and the internal dynamics, combined with previous mutation data, give important clues toward a possible mechanism for the activation of LPL by apoCII.

-	~~==Function==~~	+	Global structure and dynamics of human apolipoprotein CII in complex with micelles: evidence for increased mobility of the helix involved in the activation of lipoprotein lipase.,Zdunek J, Martinez GV, Schleucher J, Lycksell PO, Yin Y, Nilsson S, Shen Y, Olivecrona G, Wijmenga S Biochemistry. 2003 Feb 25;42(7):1872-89. PMID:12590574<ref>PMID:12590574</ref>
-	~~[[http~~:~~//www.uniprot.org/uniprot/APC2_HUMAN APC2_HUMAN]] Promotes rapid degradation~~ of ~~CTNNB1 and may function as a tumor suppressor. May function~~ in ~~Wnt signaling~~.~~<ref>PMID~~:~~9823329</ref><ref>~~PMID:~~10021369</ref>~~<ref>PMID:~~11691822~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[1o8t]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1O8T OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:012590574</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Lycksell, P O.]]		[[Category: Lycksell, P O.]]

OCA at 18:52, 24 March 2013

OCA — Sun, 24 Mar 2013 18:52:32 GMT

←Older revision		Revision as of 18:52, 24 March 2013
Line 1:		Line 1:
-	[[Image:1o8t.png\|left\|200px]]
-
	{{STRUCTURE_1o8t\| PDB=1o8t \| SCENE= }}		{{STRUCTURE_1o8t\| PDB=1o8t \| SCENE= }}
-
	===GLOBAL STRUCTURE AND DYNAMICS OF HUMAN APOLIPOPROTEIN CII IN COMPLEX WITH MICELLES: EVIDENCE FOR INCREASED MOBILITY OF THE HELIX INVOLVVED IN THE ACTIVATION OF LIPOPROTEIN LIPASE.===		===GLOBAL STRUCTURE AND DYNAMICS OF HUMAN APOLIPOPROTEIN CII IN COMPLEX WITH MICELLES: EVIDENCE FOR INCREASED MOBILITY OF THE HELIX INVOLVVED IN THE ACTIVATION OF LIPOPROTEIN LIPASE.===
		+	{{ABSTRACT_PUBMED_12590574}}

-	~~{{ABSTRACT_PUBMED_12590574}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/APC2_HUMAN APC2_HUMAN]] Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling.<ref>PMID:9823329</ref><ref>PMID:10021369</ref><ref>PMID:11691822</ref>

	==About this Structure==		==About this Structure==
Line 11:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:012590574</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012590574</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Lycksell, P O.]]		[[Category: Lycksell, P O.]]