1oai - Revision history

OCA at 08:58, 9 May 2024

OCA — Thu, 09 May 2024 08:58:30 GMT

←Older revision		Revision as of 08:58, 9 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oai ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oai ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mRNA nuclear export function of Tap/NXF1 requires interactions with nuclear pore proteins (nucleoporins) that contain characteristic Phe-Gly repeats based on FG, GLFG or FxFG cores separated by hydrophilic linkers. FG-nucleoporins bind the two most C-terminal domains of Tap, which have NTF2 and UBA folds, respectively. We used a combination of NMR and X-ray crystallography to define the interaction interface between Tap UBA and FxFG nucleoporins and show that it involves primarily the two aromatic rings of the FxFG core that bind in a hydrophobic surface depression centred on Tap Cys588. NMR evidence indicates that the same depression mediates the binding of GLFG nucleoporins, which we confirmed by demonstrating competition between the two classes of repeat for binding to Tap UBA. Moreover, modification of Cys588 reduced the binding of Tap UBA to both GLFG and FxFG nucleoporins as well as to nuclear envelopes. These data underscore the central role of the conserved FG-nucleoporin repeat cores in binding to Tap UBA and indicate that functional differences between different classes of nucleoporins depend more on their spatial distribution in nuclear pores than on their binding to different sites on Tap UBA.
		+
		+	Structural basis for the interaction between the Tap/NXF1 UBA domain and FG nucleoporins at 1A resolution.,Grant RP, Neuhaus D, Stewart M J Mol Biol. 2003 Feb 21;326(3):849-58. PMID:12581645<ref>PMID:12581645</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1oai" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:56, 10 April 2024

OCA — Wed, 10 Apr 2024 08:56:21 GMT

←Older revision		Revision as of 08:56, 10 April 2024
Line 3:		Line 3:
	<StructureSection load='1oai' size='340' side='right'caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>		<StructureSection load='1oai' size='340' side='right'caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OAI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OAI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [https://pdbe.org/1oai PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [https://www.ebi.ac.uk/pdbsum/1oai PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oai ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [https://pdbe.org/1oai PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [https://www.ebi.ac.uk/pdbsum/1oai PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oai ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NXF1_HUMAN NXF1_HUMAN]] Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5.<ref>PMID:9660949</ref> <ref>PMID:19165146</ref>	+	[https://www.uniprot.org/uniprot/NXF1_HUMAN NXF1_HUMAN] Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5.<ref>PMID:9660949</ref> <ref>PMID:19165146</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oai ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oai ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The mRNA nuclear export function of Tap/NXF1 requires interactions with nuclear pore proteins (nucleoporins) that contain characteristic Phe-Gly repeats based on FG, GLFG or FxFG cores separated by hydrophilic linkers. FG-nucleoporins bind the two most C-terminal domains of Tap, which have NTF2 and UBA folds, respectively. We used a combination of NMR and X-ray crystallography to define the interaction interface between Tap UBA and FxFG nucleoporins and show that it involves primarily the two aromatic rings of the FxFG core that bind in a hydrophobic surface depression centred on Tap Cys588. NMR evidence indicates that the same depression mediates the binding of GLFG nucleoporins, which we confirmed by demonstrating competition between the two classes of repeat for binding to Tap UBA. Moreover, modification of Cys588 reduced the binding of Tap UBA to both GLFG and FxFG nucleoporins as well as to nuclear envelopes. These data underscore the central role of the conserved FG-nucleoporin repeat cores in binding to Tap UBA and indicate that functional differences between different classes of nucleoporins depend more on their spatial distribution in nuclear pores than on their binding to different sites on Tap UBA.
-
-	Structural basis for the interaction between the Tap/NXF1 UBA domain and FG nucleoporins at 1A resolution.,Grant RP, Neuhaus D, Stewart M J Mol Biol. 2003 Feb 21;326(3):849-58. PMID:12581645<ref>PMID:12581645</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1oai" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Grant~~, R P~~]]	+	[[Category: Grant RP]]
-	[[Category: Neuhaus, D]]	+	[[Category: Neuhaus D]]
-	[[Category: Stewart, M]]	+	[[Category: Stewart M]]
-	~~[[Category: Nuclear transport]]~~	+
-	~~[[Category: Nuclear transport factor]]~~	+
-	~~[[Category: Nucleoporin~~]]	+

OCA at 15:53, 22 December 2021

OCA — Wed, 22 Dec 2021 15:53:43 GMT

←Older revision		Revision as of 15:53, 22 December 2021
Line 3:		Line 3:
	<StructureSection load='1oai' size='340' side='right'caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>		<StructureSection load='1oai' size='340' side='right'caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OAI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OAI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [~~http~~://pdbe.org/1oai PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1oai PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1oai ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [https://pdbe.org/1oai PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [https://www.ebi.ac.uk/pdbsum/1oai PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oai ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NXF1_HUMAN NXF1_HUMAN]] Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5.<ref>PMID:9660949</ref> <ref>PMID:19165146</ref>	+	[[https://www.uniprot.org/uniprot/NXF1_HUMAN NXF1_HUMAN]] Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5.<ref>PMID:9660949</ref> <ref>PMID:19165146</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:38, 18 March 2020

OCA — Wed, 18 Mar 2020 10:38:20 GMT

←Older revision		Revision as of 10:38, 18 March 2020
Line 1:		Line 1:

	==Complex between Tap UBA domain and FxFG nucleoporin peptide==		==Complex between Tap UBA domain and FxFG nucleoporin peptide==
-	<StructureSection load='1oai' size='340' side='right' caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>	+	<StructureSection load='1oai' size='340' side='right'caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OAI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OAI FirstGlance]. <br>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Grant, R P]]		[[Category: Grant, R P]]
	[[Category: Neuhaus, D]]		[[Category: Neuhaus, D]]

OCA at 07:02, 7 February 2018

OCA — Wed, 07 Feb 2018 07:02:41 GMT

←Older revision		Revision as of 07:02, 7 February 2018
Line 1:		Line 1:
-	==~~COMPLEX BETWEEN TAP~~ UBA ~~DOMAIN AND FXFG NUCLEOPORIN PEPTIDE~~==	+
		+	==Complex between Tap UBA domain and FxFG nucleoporin peptide==
	<StructureSection load='1oai' size='340' side='right' caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>		<StructureSection load='1oai' size='340' side='right' caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OAI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OAI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [http://pdbe.org/1oai PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [http://www.ebi.ac.uk/pdbsum/1oai PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [http://pdbe.org/1oai PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [http://www.ebi.ac.uk/pdbsum/1oai PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1oai ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/1oai_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/1oai_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 16:21, 9 February 2016

OCA — Tue, 09 Feb 2016 16:21:55 GMT

←Older revision		Revision as of 16:21, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oai ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:58, 10 September 2015

OCA — Thu, 10 Sep 2015 23:58:57 GMT

←Older revision		Revision as of 23:58, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1oai' size='340' side='right' caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>		<StructureSection load='1oai' size='340' side='right' caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OAI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OAI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [http://www.ebi.ac.uk/pdbsum/1oai PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [http://pdbe.org/1oai PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [http://www.ebi.ac.uk/pdbsum/1oai PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1oai" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Grant, R P]]		[[Category: Grant, R P]]
	[[Category: Neuhaus, D]]		[[Category: Neuhaus, D]]

OCA at 08:02, 25 December 2014

OCA — Thu, 25 Dec 2014 08:02:25 GMT

←Older revision		Revision as of 08:02, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OAI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OAI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [http://www.ebi.ac.uk/pdbsum/1oai PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [http://www.ebi.ac.uk/pdbsum/1oai PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NXF1_HUMAN NXF1_HUMAN]] Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5.<ref>PMID:9660949</ref> <ref>PMID:19165146</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Grant, R P.]]	+	[[Category: Grant, R P]]
-	[[Category: Neuhaus, D.]]	+	[[Category: Neuhaus, D]]
-	[[Category: Stewart, M.]]	+	[[Category: Stewart, M]]
	[[Category: Nuclear transport]]		[[Category: Nuclear transport]]
	[[Category: Nuclear transport factor]]		[[Category: Nuclear transport factor]]
	[[Category: Nucleoporin]]		[[Category: Nucleoporin]]

OCA at 14:14, 28 September 2014

OCA — Sun, 28 Sep 2014 14:14:10 GMT

←Older revision		Revision as of 14:14, 28 September 2014
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-	[[~~Image~~:1oai.~~png\|left~~\|200px]]	+	==COMPLEX BETWEEN TAP UBA DOMAIN AND FXFG NUCLEOPORIN PEPTIDE==
		+	<StructureSection load='1oai' size='340' side='right' caption='[[1oai]], [[Resolution\|resolution]] 1.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1oai]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OAI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oai OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oai RCSB], [http://www.ebi.ac.uk/pdbsum/1oai PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/1oai_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mRNA nuclear export function of Tap/NXF1 requires interactions with nuclear pore proteins (nucleoporins) that contain characteristic Phe-Gly repeats based on FG, GLFG or FxFG cores separated by hydrophilic linkers. FG-nucleoporins bind the two most C-terminal domains of Tap, which have NTF2 and UBA folds, respectively. We used a combination of NMR and X-ray crystallography to define the interaction interface between Tap UBA and FxFG nucleoporins and show that it involves primarily the two aromatic rings of the FxFG core that bind in a hydrophobic surface depression centred on Tap Cys588. NMR evidence indicates that the same depression mediates the binding of GLFG nucleoporins, which we confirmed by demonstrating competition between the two classes of repeat for binding to Tap UBA. Moreover, modification of Cys588 reduced the binding of Tap UBA to both GLFG and FxFG nucleoporins as well as to nuclear envelopes. These data underscore the central role of the conserved FG-nucleoporin repeat cores in binding to Tap UBA and indicate that functional differences between different classes of nucleoporins depend more on their spatial distribution in nuclear pores than on their binding to different sites on Tap UBA.

-	~~{{STRUCTURE_1oai\| PDB=1oai \| SCENE= }}~~	+	Structural basis for the interaction between the Tap/NXF1 UBA domain and FG nucleoporins at 1A resolution.,Grant RP, Neuhaus D, Stewart M J Mol Biol. 2003 Feb 21;326(3):849-58. PMID:12581645<ref>PMID:12581645</ref>

-	~~===COMPLEX BETWEEN TAP UBA DOMAIN AND FXFG NUCLEOPORIN PEPTIDE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12581645}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1oai]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1OAI OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012581645<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Grant, R P.]]		[[Category: Grant, R P.]]

OCA: Protected "1oai" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2012 21:45:17 GMT

Protected "1oai" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:45, 12 December 2012