1obl - Revision history

OCA at 12:34, 13 December 2023

OCA — Wed, 13 Dec 2023 12:34:03 GMT

←Older revision		Revision as of 12:34, 13 December 2023
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	<StructureSection load='1obl' size='340' side='right'caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1obl' size='340' side='right'caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"rhizobacterium_japonicum"_kirchner_1896 "rhizobacterium~~ japonicum~~" kirchner 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OBL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OBL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [https://pdbe.org/1obl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [https://www.ebi.ac.uk/pdbsum/1obl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1obl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [https://pdbe.org/1obl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [https://www.ebi.ac.uk/pdbsum/1obl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1obl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9ZIV5_BRAJP Q9ZIV5_BRAJP]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rhizobacterium~~ japonicum ~~kirchner 1896~~]]	+	[[Category: Bradyrhizobium japonicum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Oh, B H]]	+	[[Category: Oh B-H]]
-	[[Category: Shin, S]]	+	[[Category: Shin S]]
-	~~[[Category: Amidase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Malonamidase]]~~	+
-	~~[[Category: Mutant~~]]	+

OCA at 07:11, 25 August 2021

OCA — Wed, 25 Aug 2021 07:11:07 GMT

←Older revision		Revision as of 07:11, 25 August 2021
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	<StructureSection load='1obl' size='340' side='right'caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1obl' size='340' side='right'caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"rhizobacterium_japonicum"_kirchner_1896 "rhizobacterium japonicum" kirchner 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OBL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"rhizobacterium_japonicum"_kirchner_1896 "rhizobacterium japonicum" kirchner 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OBL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [~~http~~://pdbe.org/1obl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1obl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1obl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [https://pdbe.org/1obl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [https://www.ebi.ac.uk/pdbsum/1obl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1obl ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 18:32, 20 November 2019

OCA — Wed, 20 Nov 2019 18:32:58 GMT

←Older revision		Revision as of 18:32, 20 November 2019
Line 1:		Line 1:

	==crystal structure of the S133A mutant of Malonamidase E2 complexed with malonate from Bradyrhizobium japonicum==		==crystal structure of the S133A mutant of Malonamidase E2 complexed with malonate from Bradyrhizobium japonicum==
-	<StructureSection load='1obl' size='340' side='right' caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1obl' size='340' side='right'caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhizobacterium_japonicum"_kirchner_1896 "rhizobacterium japonicum" kirchner 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhizobacterium_japonicum"_kirchner_1896 "rhizobacterium japonicum" kirchner 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBL FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Rhizobacterium japonicum kirchner 1896]]		[[Category: Rhizobacterium japonicum kirchner 1896]]
		+	[[Category: Large Structures]]
	[[Category: Oh, B H]]		[[Category: Oh, B H]]
	[[Category: Shin, S]]		[[Category: Shin, S]]

OCA at 07:19, 7 February 2018

OCA — Wed, 07 Feb 2018 07:19:59 GMT

←Older revision		Revision as of 07:19, 7 February 2018
Line 1:		Line 1:
		+
	==crystal structure of the S133A mutant of Malonamidase E2 complexed with malonate from Bradyrhizobium japonicum==		==crystal structure of the S133A mutant of Malonamidase E2 complexed with malonate from Bradyrhizobium japonicum==
	<StructureSection load='1obl' size='340' side='right' caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1obl' size='340' side='right' caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [http://pdbe.org/1obl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [http://www.ebi.ac.uk/pdbsum/1obl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [http://pdbe.org/1obl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [http://www.ebi.ac.uk/pdbsum/1obl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1obl ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/1obl_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/1obl_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 02:50, 7 February 2016

OCA — Sun, 07 Feb 2016 02:50:03 GMT

←Older revision		Revision as of 02:50, 7 February 2016
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [http://www.ebi.ac.uk/pdbsum/1obl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [http://pdbe.org/1obl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [http://www.ebi.ac.uk/pdbsum/1obl PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1obl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1obl" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:30, 22 April 2015

OCA — Wed, 22 Apr 2015 06:30:34 GMT

←Older revision		Revision as of 06:30, 22 April 2015
Line 1:		Line 1:
-	==~~CRYSTAL STRUCTURE OF THE~~ S133A ~~MUTANT OF MALONAMIDASE~~ E2 ~~COMPLEXED WITH MALONATE FROM BRADYRHIZOBIUM JAPONICUM~~==	+	==crystal structure of the S133A mutant of Malonamidase E2 complexed with malonate from Bradyrhizobium japonicum==
	<StructureSection load='1obl' size='340' side='right' caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1obl' size='340' side='right' caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bradyrhizobium_japonicum Bradyrhizobium~~ japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhizobacterium_japonicum"_kirchner_1896 "rhizobacterium japonicum" kirchner 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</td></tr>
Line 29:		Line 29:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bradyrhizobium~~ japonicum]]	+	[[Category: Rhizobacterium japonicum kirchner 1896]]
	[[Category: Oh, B H]]		[[Category: Oh, B H]]
	[[Category: Shin, S]]		[[Category: Shin, S]]
	[[Category: Amidase]]		[[Category: Amidase]]
		+	[[Category: Hydrolase]]
	[[Category: Malonamidase]]		[[Category: Malonamidase]]
	[[Category: Mutant]]		[[Category: Mutant]]

OCA at 15:49, 5 January 2015

OCA — Mon, 05 Jan 2015 15:49:15 GMT

←Older revision		Revision as of 15:49, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [http://www.ebi.ac.uk/pdbsum/1obl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [http://www.ebi.ac.uk/pdbsum/1obl PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Bradyrhizobium japonicum]]		[[Category: Bradyrhizobium japonicum]]
-	[[Category: Oh, B H.]]	+	[[Category: Oh, B H]]
-	[[Category: Shin, S.]]	+	[[Category: Shin, S]]
	[[Category: Amidase]]		[[Category: Amidase]]
	[[Category: Malonamidase]]		[[Category: Malonamidase]]
	[[Category: Mutant]]		[[Category: Mutant]]

OCA at 13:20, 28 September 2014

OCA — Sun, 28 Sep 2014 13:20:37 GMT

←Older revision		Revision as of 13:20, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1obl.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE S133A MUTANT OF MALONAMIDASE E2 COMPLEXED WITH MALONATE FROM BRADYRHIZOBIUM JAPONICUM==
		+	<StructureSection load='1obl' size='340' side='right' caption='[[1obl]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1obl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gr8\|1gr8]], [[1gr9\|1gr9]], [[1grk\|1grk]], [[1o9n\|1o9n]], [[1o9o\|1o9o]], [[1o9p\|1o9p]], [[1o9q\|1o9q]], [[1obi\|1obi]], [[1obj\|1obj]], [[1obk\|1obk]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1obl RCSB], [http://www.ebi.ac.uk/pdbsum/1obl PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/1obl_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Amidase signature family enzymes, which are widespread in nature, contain a newly identified Ser-cisSer-Lys catalytic triad in which the peptide bond between Ser131 and the preceding residue Gly130 is in a cis configuration. In order to characterize the property of the novel triad, we have determined the structures of five mutant malonamidase E2 enzymes that contain a Cys-cisSer-Lys, Ser-cisAla-Lys, or Ser-cisSer-Ala triad or a substitution of Gly130 with alanine. Cysteine cannot replace the role of Ser155 due to a hyper-reactivity of the residue, which results in the modification of the cysteine to cysteinyl sulfinic acid, most likely inside the expression host cells. The lysine residue plays a structural as well as a catalytic role, since the substitution of the residue with alanine disrupts the active site structure completely. The two observations are in sharp contrast with the consequences of the corresponding substitutions in the classical Ser-His-Asp triad. Structural data on the mutant containing the Ser-cisAla-Lys triad convincingly suggest that Ser131 plays an analogous catalytic role as the histidine of the Ser-His-Asp triad. The unusual cis configuration of Ser131 appears essential for the precise contacts of this residue with the other triad residues, as indicated by the near invariance of the preceding glycine residue (Gly130), structural data on the G130A mutant, and by a modeling experiment. The data provide a deep understanding of the role of each residue of the new triad at the atomic level and demonstrate that the new triad is a catalytic device distinctively different from the classical triad or its variants.

-	~~{{STRUCTURE_1obl\| PDB=1obl \| SCENE= }}~~	+	Characterization of a novel Ser-cisSer-Lys catalytic triad in comparison with the classical Ser-His-Asp triad.,Shin S, Yun YS, Koo HM, Kim YS, Choi KY, Oh BH J Biol Chem. 2003 Jul 4;278(27):24937-43. Epub 2003 Apr 23. PMID:12711609<ref>PMID:12711609</ref>

-	~~===CRYSTAL STRUCTURE OF THE S133A MUTANT OF MALONAMIDASE E2 COMPLEXED WITH MALONATE FROM BRADYRHIZOBIUM JAPONICUM===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12711609}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1obl]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012711609<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bradyrhizobium japonicum]]		[[Category: Bradyrhizobium japonicum]]
	[[Category: Oh, B H.]]		[[Category: Oh, B H.]]

OCA: Protected "1obl" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2012 21:39:55 GMT

Protected "1obl" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:39, 12 December 2012

OCA at 21:39, 12 December 2012

OCA — Wed, 12 Dec 2012 21:39:52 GMT

←Older revision		Revision as of 21:39, 12 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1obl.png\|left\|200px]]		[[Image:1obl.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1obl", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1obl\| PDB=1obl \| SCENE= }}		{{STRUCTURE_1obl\| PDB=1obl \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE S133A MUTANT OF MALONAMIDASE E2 COMPLEXED WITH MALONATE FROM BRADYRHIZOBIUM JAPONICUM===		===CRYSTAL STRUCTURE OF THE S133A MUTANT OF MALONAMIDASE E2 COMPLEXED WITH MALONATE FROM BRADYRHIZOBIUM JAPONICUM===

		+	{{ABSTRACT_PUBMED_12711609}}

	==About this Structure==		==About this Structure==
-	~~1OBL~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA].	+	[[1obl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBL OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:012711609</ref><references group="xtra"/>
	[[Category: Bradyrhizobium japonicum]]		[[Category: Bradyrhizobium japonicum]]
	[[Category: Oh, B H.]]		[[Category: Oh, B H.]]
Line 21:		Line 18:
	[[Category: Malonamidase]]		[[Category: Malonamidase]]
	[[Category: Mutant]]		[[Category: Mutant]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 10:31:08 2009''