1obn - Revision history

OCA at 12:34, 13 December 2023

OCA — Wed, 13 Dec 2023 12:34:07 GMT

←Older revision		Revision as of 12:34, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='1obn' size='340' side='right'caption='[[1obn]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='1obn' size='340' side='right'caption='[[1obn]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1obn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~A._nidulans A.~~ nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1OBN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1obn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OBN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk0\|1bk0]], [[1blz\|1blz]], [[1hb1\|1hb1]], [[1hb2\|1hb2]], [[1hb3\|1hb3]], [[1hb4\|1hb4]], [[1ips\|1ips]], [[1oc1\|1oc1]], [[1qiq\|1qiq]], [[1qje\|1qje]], [[1qjf\|1qjf]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [~~http~~://pdbe.org/1obn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1obn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1obn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [https://pdbe.org/1obn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [https://www.ebi.ac.uk/pdbsum/1obn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1obn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~IPNS_EMENI IPNS_EMENI~~]~~] Removes, in~~ the ~~presence~~ of ~~oxygen~~, ~~4 hydrogen atoms from delta~~-L-(~~alpha~~-~~aminoadipyl~~)-L-cysteinyl-D-valine (ACV) to ~~form~~ the ~~azetidinone and thiazolidine rings of~~ isopenicillin.	+	[https://www.uniprot.org/uniprot/IPNA_EMENI IPNA_EMENI] Isopenicillin N synthase; part of the gene cluster that mediates the biosynthesis of penicillin, the world's most important antibiotic (PubMed:3319778, PubMed:11755401). IpnA catalyzes the cyclization of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) to form isopenicillin N (IPN) that contains the beta-lactam nucleus (PubMed:3319778, PubMed:11755401, PubMed:28703303). The penicillin biosynthesis occurs via 3 enzymatic steps, the first corresponding to the production of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) by the NRPS acvA. The tripeptide ACV is then cyclized to isopenicillin N (IPN) by the isopenicillin N synthase ipnA that forms the beta-lactam nucleus. Finally, the alpha-aminoadipyl side chain is exchanged for phenylacetic acid by the isopenicillin N acyltransferase penDE to yield penicillin in the peroxisomal matrix (By similarity).[UniProtKB:P08703]<ref>PMID:11755401</ref> <ref>PMID:28703303</ref> <ref>PMID:3319778</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: A. nidulans]]	+	[[Category: Aspergillus nidulans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baldwin~~, J E~~]]	+	[[Category: Baldwin JE]]
-	[[Category: Clifton~~, I J~~]]	+	[[Category: Clifton IJ]]
-	[[Category: Long~~, A J~~]]	+	[[Category: Long AJ]]
-	[[Category: Roach~~, P L~~]]	+	[[Category: Roach PL]]
-	[[Category: Rutledge~~, P J~~]]	+	[[Category: Rutledge PJ]]
-	[[Category: Schofield~~, C J]]~~	+	[[Category: Schofield CJ]]
-	~~[[Category: B-lactam antibiotic]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Oxygenase]]~~	+
-	~~[[Category: Penicillin biosynthesis~~]]	+

OCA at 11:48, 29 July 2020

OCA — Wed, 29 Jul 2020 11:48:47 GMT

←Older revision		Revision as of 11:48, 29 July 2020
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	==ISOPENICILLIN N SYNTHASE aminoadipoyl-cysteinyl-aminobutyrate-FE-NO COMPLEX==		==ISOPENICILLIN N SYNTHASE aminoadipoyl-cysteinyl-aminobutyrate-FE-NO COMPLEX==
-	<StructureSection load='1obn' size='340' side='right' caption='[[1obn]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='1obn' size='340' side='right'caption='[[1obn]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1obn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/A._nidulans A. nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OBN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1obn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/A._nidulans A. nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1OBN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk0\|1bk0]], [[1blz\|1blz]], [[1hb1\|1hb1]], [[1hb2\|1hb2]], [[1hb3\|1hb3]], [[1hb4\|1hb4]], [[1ips\|1ips]], [[1oc1\|1oc1]], [[1qiq\|1qiq]], [[1qje\|1qje]], [[1qjf\|1qjf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk0\|1bk0]], [[1blz\|1blz]], [[1hb1\|1hb1]], [[1hb2\|1hb2]], [[1hb3\|1hb3]], [[1hb4\|1hb4]], [[1ips\|1ips]], [[1oc1\|1oc1]], [[1qiq\|1qiq]], [[1qje\|1qje]], [[1qjf\|1qjf]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [http://pdbe.org/1obn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [http://www.ebi.ac.uk/pdbsum/1obn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1obn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [http://pdbe.org/1obn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [http://www.ebi.ac.uk/pdbsum/1obn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1obn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: A. nidulans]]		[[Category: A. nidulans]]
		+	[[Category: Large Structures]]
	[[Category: Baldwin, J E]]		[[Category: Baldwin, J E]]
	[[Category: Clifton, I J]]		[[Category: Clifton, I J]]

OCA at 06:09, 24 October 2018

OCA — Wed, 24 Oct 2018 06:09:30 GMT

←Older revision		Revision as of 06:09, 24 October 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/1obn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/1obn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 09:56, 1 November 2017

OCA — Wed, 01 Nov 2017 09:56:56 GMT

←Older revision		Revision as of 09:56, 1 November 2017
Line 1:		Line 1:
		+
	==ISOPENICILLIN N SYNTHASE aminoadipoyl-cysteinyl-aminobutyrate-FE-NO COMPLEX==		==ISOPENICILLIN N SYNTHASE aminoadipoyl-cysteinyl-aminobutyrate-FE-NO COMPLEX==
	<StructureSection load='1obn' size='340' side='right' caption='[[1obn]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='1obn' size='340' side='right' caption='[[1obn]], [[Resolution\|resolution]] 1.30Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk0\|1bk0]], [[1blz\|1blz]], [[1hb1\|1hb1]], [[1hb2\|1hb2]], [[1hb3\|1hb3]], [[1hb4\|1hb4]], [[1ips\|1ips]], [[1oc1\|1oc1]], [[1qiq\|1qiq]], [[1qje\|1qje]], [[1qjf\|1qjf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk0\|1bk0]], [[1blz\|1blz]], [[1hb1\|1hb1]], [[1hb2\|1hb2]], [[1hb3\|1hb3]], [[1hb4\|1hb4]], [[1ips\|1ips]], [[1oc1\|1oc1]], [[1qiq\|1qiq]], [[1qje\|1qje]], [[1qjf\|1qjf]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [http://pdbe.org/1obn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [http://www.ebi.ac.uk/pdbsum/1obn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [http://pdbe.org/1obn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [http://www.ebi.ac.uk/pdbsum/1obn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1obn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1obn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:26, 11 September 2015

OCA — Fri, 11 Sep 2015 12:26:18 GMT

←Older revision		Revision as of 12:26, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1obn' size='340' side='right' caption='[[1obn]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='1obn' size='340' side='right' caption='[[1obn]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1obn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Emericella_nidulans Emericella~~ nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1obn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/A._nidulans A. nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk0\|1bk0]], [[1blz\|1blz]], [[1hb1\|1hb1]], [[1hb2\|1hb2]], [[1hb3\|1hb3]], [[1hb4\|1hb4]], [[1ips\|1ips]], [[1oc1\|1oc1]], [[1qiq\|1qiq]], [[1qje\|1qje]], [[1qjf\|1qjf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk0\|1bk0]], [[1blz\|1blz]], [[1hb1\|1hb1]], [[1hb2\|1hb2]], [[1hb3\|1hb3]], [[1hb4\|1hb4]], [[1ips\|1ips]], [[1oc1\|1oc1]], [[1qiq\|1qiq]], [[1qje\|1qje]], [[1qjf\|1qjf]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [http://www.ebi.ac.uk/pdbsum/1obn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [http://pdbe.org/1obn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [http://www.ebi.ac.uk/pdbsum/1obn PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1obn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Emericella~~ nidulans]]	+	[[Category: A. nidulans]]
	[[Category: Baldwin, J E]]		[[Category: Baldwin, J E]]
	[[Category: Clifton, I J]]		[[Category: Clifton, I J]]

OCA at 16:25, 25 December 2014

OCA — Thu, 25 Dec 2014 16:25:50 GMT

←Older revision		Revision as of 16:25, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1obn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1obn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk0\|1bk0]], [[1blz\|1blz]], [[1hb1\|1hb1]], [[1hb2\|1hb2]], [[1hb3\|1hb3]], [[1hb4\|1hb4]], [[1ips\|1ips]], [[1oc1\|1oc1]], [[1qiq\|1qiq]], [[1qje\|1qje]], [[1qjf\|1qjf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk0\|1bk0]], [[1blz\|1blz]], [[1hb1\|1hb1]], [[1hb2\|1hb2]], [[1hb3\|1hb3]], [[1hb4\|1hb4]], [[1ips\|1ips]], [[1oc1\|1oc1]], [[1qiq\|1qiq]], [[1qje\|1qje]], [[1qjf\|1qjf]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [http://www.ebi.ac.uk/pdbsum/1obn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [http://www.ebi.ac.uk/pdbsum/1obn PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IPNS_EMENI IPNS_EMENI]] Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Emericella nidulans]]		[[Category: Emericella nidulans]]
-	[[Category: Baldwin, J E.]]	+	[[Category: Baldwin, J E]]
-	[[Category: Clifton, I J.]]	+	[[Category: Clifton, I J]]
-	[[Category: Long, A J.]]	+	[[Category: Long, A J]]
-	[[Category: Roach, P L.]]	+	[[Category: Roach, P L]]
-	[[Category: Rutledge, P J.]]	+	[[Category: Rutledge, P J]]
-	[[Category: Schofield, C J.]]	+	[[Category: Schofield, C J]]
	[[Category: B-lactam antibiotic]]		[[Category: B-lactam antibiotic]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Oxygenase]]		[[Category: Oxygenase]]
	[[Category: Penicillin biosynthesis]]		[[Category: Penicillin biosynthesis]]

OCA at 15:14, 29 September 2014

OCA — Mon, 29 Sep 2014 15:14:04 GMT

←Older revision		Revision as of 15:14, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1obn\| PDB=1obn \| SCENE= }}~~	+	==ISOPENICILLIN N SYNTHASE aminoadipoyl-cysteinyl-aminobutyrate-FE-NO COMPLEX==
-	===ISOPENICILLIN N SYNTHASE aminoadipoyl-cysteinyl-aminobutyrate-FE-NO COMPLEX===	+	<StructureSection load='1obn' size='340' side='right' caption='[[1obn]], [[Resolution\|resolution]] 1.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_12622704}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1obn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OBN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASV:DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VINYLGLYCINE'>ASV</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HOA:HYDROXYAMINE'>HOA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk0\|1bk0]], [[1blz\|1blz]], [[1hb1\|1hb1]], [[1hb2\|1hb2]], [[1hb3\|1hb3]], [[1hb4\|1hb4]], [[1ips\|1ips]], [[1oc1\|1oc1]], [[1qiq\|1qiq]], [[1qje\|1qje]], [[1qjf\|1qjf]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1obn RCSB], [http://www.ebi.ac.uk/pdbsum/1obn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/1obn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Isopenicillin N synthase (IPNS) is a non-haem iron(II) oxidase which catalyses the biosynthesis of isopenicillin N from the tripeptide delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV). Herein we report crystallographic studies to investigate the reaction of IPNS with the truncated substrate analogue delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate (ACAb). It has been reported previously that this analogue gives rise to three beta-lactam products when incubated with IPNS: two methyl penams and a cepham. Crystal structures of the IPNS-Fe(II)-ACAb and IPNS-Fe(II)-ACAb-NO complexes have now been solved and are reported herein. These structures and modelling studies based on them shed light on the diminished product selectivity shown by IPNS in its reaction with ACAb and further rationalize the presence of certain key residues at the IPNS active site.

-	~~==About this Structure==~~	+	Structural studies on the reaction of isopenicillin N synthase with the substrate analogue delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-alpha-aminobutyrate.,Long AJ, Clifton IJ, Roach PL, Baldwin JE, Schofield CJ, Rutledge PJ Biochem J. 2003 Jun 15;372(Pt 3):687-93. PMID:12622704<ref>PMID:12622704</ref>
-	~~[[1obn]] is a 1 chain structure~~ with ~~sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Emericella_nidulans Emericella nidulans]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1OBN OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Isopenicillin N synthase\|Isopenicillin N synthase]]		*[[Isopenicillin N synthase\|Isopenicillin N synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012622704<~~/~~ref~~><~~ref group="xtra">PMID:009194566</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Emericella nidulans]]		[[Category: Emericella nidulans]]
	[[Category: Baldwin, J E.]]		[[Category: Baldwin, J E.]]

OCA at 10:35, 11 March 2013

OCA — Mon, 11 Mar 2013 10:35:48 GMT

←Older revision		Revision as of 10:35, 11 March 2013
Line 1:		Line 1:
-	[[Image:1obn.png\|left\|200px]]
-
	{{STRUCTURE_1obn\| PDB=1obn \| SCENE= }}		{{STRUCTURE_1obn\| PDB=1obn \| SCENE= }}
-
	===ISOPENICILLIN N SYNTHASE aminoadipoyl-cysteinyl-aminobutyrate-FE-NO COMPLEX===		===ISOPENICILLIN N SYNTHASE aminoadipoyl-cysteinyl-aminobutyrate-FE-NO COMPLEX===
-
	{{ABSTRACT_PUBMED_12622704}}		{{ABSTRACT_PUBMED_12622704}}

OCA at 12:44, 30 January 2013

OCA — Wed, 30 Jan 2013 12:44:18 GMT

←Older revision		Revision as of 12:44, 30 January 2013
Line 3:		Line 3:
	{{STRUCTURE_1obn\| PDB=1obn \| SCENE= }}		{{STRUCTURE_1obn\| PDB=1obn \| SCENE= }}

-	===ISOPENICILLIN N SYNTHASE ~~AMINOADIPOYL~~-~~CYSTEINYL~~-~~AMINOBUTYRATE~~-FE-NO COMPLEX===	+	===ISOPENICILLIN N SYNTHASE aminoadipoyl-cysteinyl-aminobutyrate-FE-NO COMPLEX===

	{{ABSTRACT_PUBMED_12622704}}		{{ABSTRACT_PUBMED_12622704}}
Line 9:		Line 9:
	==About this Structure==		==About this Structure==
	[[1obn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA].		[[1obn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBN OCA].
		+
		+	==See Also==
		+	*[[Isopenicillin N synthase\|Isopenicillin N synthase]]

	==Reference==		==Reference==

OCA: Protected "1obn" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2012 21:55:06 GMT

Protected "1obn" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:55, 12 December 2012