1ocv - Revision history

OCA at 12:35, 13 December 2023

OCA — Wed, 13 Dec 2023 12:35:43 GMT

←Older revision		Revision as of 12:35, 13 December 2023
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	<StructureSection load='1ocv' size='340' side='right'caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ocv' size='340' side='right'caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_11996 Atcc 11996~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OCV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OCV FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1buq\|1buq]], [[1isk\|1isk]], [[1qjg\|1qjg]], [[8cho\|8cho]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Steroid_Delta~~-~~isomerase Steroid Delta-isomerase]~~, ~~with EC number~~ [~~https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1~~] ~~</span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [https://pdbe.org/1ocv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [https://www.ebi.ac.uk/pdbsum/1ocv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ocv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [https://pdbe.org/1ocv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [https://www.ebi.ac.uk/pdbsum/1ocv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ocv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SDIS_COMTE SDIS_COMTE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 11996~~]]	+	[[Category: Comamonas testosteroni]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Steroid Delta-isomerase]]~~	+	[[Category: Lee T-H]]
-	[[Category: Lee, T H]]	+	[[Category: Shin S]]
-	[[Category: Shin, S]]	+	[[Category: Yun YS]]
-	[[Category: Yun~~, Y S]]~~	+
-	~~[[Category: Delta-5-3-ketosteroid]]~~	+
-	~~[[Category: Ketosteroid isomerase~~]]	+

OCA at 07:11, 25 August 2021

OCA — Wed, 25 Aug 2021 07:11:40 GMT

←Older revision		Revision as of 07:11, 25 August 2021
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	<StructureSection load='1ocv' size='340' side='right'caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ocv' size='340' side='right'caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_11996 Atcc 11996]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OCV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_11996 Atcc 11996]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OCV FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1buq\|1buq]], [[1isk\|1isk]], [[1qjg\|1qjg]], [[8cho\|8cho]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1buq\|1buq]], [[1isk\|1isk]], [[1qjg\|1qjg]], [[8cho\|8cho]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [~~http~~://pdbe.org/1ocv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ocv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ocv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [https://pdbe.org/1ocv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [https://www.ebi.ac.uk/pdbsum/1ocv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ocv ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 18:52, 20 November 2019

OCA — Wed, 20 Nov 2019 18:52:55 GMT

←Older revision		Revision as of 18:52, 20 November 2019
Line 1:		Line 1:

	==the F116W mutant structure of ketosteroid isomerase from Comamonas testosteroni==		==the F116W mutant structure of ketosteroid isomerase from Comamonas testosteroni==
-	<StructureSection load='1ocv' size='340' side='right' caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1ocv' size='340' side='right'caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11996 Atcc 11996]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OCV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11996 Atcc 11996]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OCV FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 1ocv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ocv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ketosteroid Isomerase\|Ketosteroid Isomerase]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 11996]]		[[Category: Atcc 11996]]
		+	[[Category: Large Structures]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
	[[Category: Lee, T H]]		[[Category: Lee, T H]]

OCA at 07:38, 7 February 2018

OCA — Wed, 07 Feb 2018 07:38:28 GMT

←Older revision		Revision as of 07:38, 7 February 2018
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-	==~~THE~~ F116W ~~MUTANT STRUCTURE OF KETOSTEROID ISOMERASE FROM COMAMONAS TESTOSTERONI~~==	+
		+	==the F116W mutant structure of ketosteroid isomerase from Comamonas testosteroni==
	<StructureSection load='1ocv' size='340' side='right' caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ocv' size='340' side='right' caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1buq\|1buq]], [[1isk\|1isk]], [[1qjg\|1qjg]], [[8cho\|8cho]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1buq\|1buq]], [[1isk\|1isk]], [[1qjg\|1qjg]], [[8cho\|8cho]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [http://pdbe.org/1ocv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [http://www.ebi.ac.uk/pdbsum/1ocv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [http://pdbe.org/1ocv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [http://www.ebi.ac.uk/pdbsum/1ocv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ocv ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oc/1ocv_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oc/1ocv_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1ocv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ocv" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Ketosteroid Isomerase\|Ketosteroid Isomerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:25, 9 February 2016

OCA — Tue, 09 Feb 2016 12:25:41 GMT

←Older revision		Revision as of 12:25, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ocv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:14, 10 September 2015

OCA — Thu, 10 Sep 2015 22:14:13 GMT

←Older revision		Revision as of 22:14, 10 September 2015
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	<StructureSection load='1ocv' size='340' side='right' caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ocv' size='340' side='right' caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Comamonas_testosteroni Comamonas testosteroni~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OCV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11996 Atcc 11996]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OCV FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1buq\|1buq]], [[1isk\|1isk]], [[1qjg\|1qjg]], [[8cho\|8cho]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1buq\|1buq]], [[1isk\|1isk]], [[1qjg\|1qjg]], [[8cho\|8cho]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [http://www.ebi.ac.uk/pdbsum/1ocv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [http://pdbe.org/1ocv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [http://www.ebi.ac.uk/pdbsum/1ocv PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ocv" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Comamonas testosteroni~~]]	+	[[Category: Atcc 11996]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
	[[Category: Lee, T H]]		[[Category: Lee, T H]]

OCA at 17:11, 5 January 2015

OCA — Mon, 05 Jan 2015 17:11:51 GMT

←Older revision		Revision as of 17:11, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OCV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OCV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1buq\|1buq]], [[1isk\|1isk]], [[1qjg\|1qjg]], [[8cho\|8cho]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1buq\|1buq]], [[1isk\|1isk]], [[1qjg\|1qjg]], [[8cho\|8cho]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [http://www.ebi.ac.uk/pdbsum/1ocv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [http://www.ebi.ac.uk/pdbsum/1ocv PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Comamonas testosteroni]]		[[Category: Comamonas testosteroni]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
-	[[Category: Lee, T H.]]	+	[[Category: Lee, T H]]
-	[[Category: Shin, S.]]	+	[[Category: Shin, S]]
-	[[Category: Yun, Y S.]]	+	[[Category: Yun, Y S]]
	[[Category: Delta-5-3-ketosteroid]]		[[Category: Delta-5-3-ketosteroid]]
	[[Category: Ketosteroid isomerase]]		[[Category: Ketosteroid isomerase]]

OCA at 18:46, 28 September 2014

OCA — Sun, 28 Sep 2014 18:46:19 GMT

←Older revision		Revision as of 18:46, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ocv.~~png~~\|~~left~~\|200px]]	+	==THE F116W MUTANT STRUCTURE OF KETOSTEROID ISOMERASE FROM COMAMONAS TESTOSTERONI==
		+	<StructureSection load='1ocv' size='340' side='right' caption='[[1ocv]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ocv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OCV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1buq\|1buq]], [[1isk\|1isk]], [[1qjg\|1qjg]], [[8cho\|8cho]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ocv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ocv RCSB], [http://www.ebi.ac.uk/pdbsum/1ocv PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oc/1ocv_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Two homologous Delta5-3-ketosteroid isomerases from Comamonas testosteroni (TI-WT) and Pseudomonas putida biotype B (PI-WT) exhibit different pH activity profiles. TI-WT loses activity below pH 5.0 due to the protonation of the conserved catalytic base, Asp-38, while PI-WT does not. Based on the structural analysis of PI-WT, the critical catalytic base, Asp-38, was found to form a hydrogen bond with the indole ring NH of Trp-116, which is homologously replaced with Phe-116 in TI-WT. To investigate the role of Trp-116, we prepared the F116W mutant of TI-WT (TI-F116W) and the W116F mutant of PI-WT (PI-W116F) and compared kinetic parameters of those mutants at different pH levels. PI-W116F exhibited significantly decreased catalytic activity at acidic pH like TI-WT, whereas TI-F116W maintained catalytic activity at acidic pH like PI-WT and increased the kcat/Km value by 2.5- to 4.7-fold compared with TI-WT at pH 3.8. The crystal structure of TI-F116W clearly showed that the indole ring NH of Trp-116 could form a hydrogen bond with the carboxyl oxygen of Asp-38 like that of PI-WT. The present results demonstrate that the activities of both PI-WT and TI-F116W at low pH were maintained by a tryptophan, which was able not only to lower the pKa value of the catalytic base but also to increase the substrate affinity. This is one example of the strategy nature can adopt to evolve the diversity of the catalytic function in the enzymes. Our results provide insight into deciphering the molecular evolution of the enzyme and creating novel enzymes by protein engineering.

-	~~{{STRUCTURE_1ocv\| PDB=1ocv \| SCENE= }}~~	+	Origin of the different pH activity profile in two homologous ketosteroid isomerases.,Yun YS, Lee TH, Nam GH, Jang DS, Shin S, Oh BH, Choi KY J Biol Chem. 2003 Jul 25;278(30):28229-36. Epub 2003 May 6. PMID:12734184<ref>PMID:12734184</ref>

-	~~===THE F116W MUTANT STRUCTURE OF KETOSTEROID ISOMERASE FROM COMAMONAS TESTOSTERONI===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_12734184}}~~	+	==See Also==
-		+	*[[Ketosteroid Isomerase\|Ketosteroid Isomerase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1ocv~~]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:012734184<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Comamonas testosteroni]]		[[Category: Comamonas testosteroni]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]

OCA: Protected "1ocv" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2012 22:17:27 GMT

Protected "1ocv" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:17, 12 December 2012

OCA at 22:17, 12 December 2012

OCA — Wed, 12 Dec 2012 22:17:24 GMT

←Older revision		Revision as of 22:17, 12 December 2012
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-	{{Seed}}
	[[Image:1ocv.png\|left\|200px]]		[[Image:1ocv.png\|left\|200px]]

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	{{STRUCTURE_1ocv\| PDB=1ocv \| SCENE= }}		{{STRUCTURE_1ocv\| PDB=1ocv \| SCENE= }}

	===THE F116W MUTANT STRUCTURE OF KETOSTEROID ISOMERASE FROM COMAMONAS TESTOSTERONI===		===THE F116W MUTANT STRUCTURE OF KETOSTEROID ISOMERASE FROM COMAMONAS TESTOSTERONI===

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-	<!--
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	{{ABSTRACT_PUBMED_12734184}}		{{ABSTRACT_PUBMED_12734184}}

	==About this Structure==		==About this Structure==
-	~~1OCV~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA].	+	[[1ocv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCV OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12734184~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012734184</ref><references group="xtra"/>
	[[Category: Comamonas testosteroni]]		[[Category: Comamonas testosteroni]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
Line 31:		Line 19:
	[[Category: Delta-5-3-ketosteroid]]		[[Category: Delta-5-3-ketosteroid]]
	[[Category: Ketosteroid isomerase]]		[[Category: Ketosteroid isomerase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 09:46:15 2009''