1ofz - Revision history

OCA at 08:59, 9 May 2024

OCA — Thu, 09 May 2024 08:59:46 GMT

←Older revision		Revision as of 08:59, 9 May 2024
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ofz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ofz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aleuria aurantia lectin is a fungal protein composed of two identical 312-amino acid subunits that specifically recognizes fucosylated glycans. The crystal structure of the lectin complexed with fucose reveals that each monomer consists of a six-bladed beta-propeller fold and of a small antiparallel two-stranded beta-sheet that plays a role in dimerization. Five fucose residues were located in binding pockets between the adjacent propeller blades. Due to repeats in the amino acid sequence, there are strong similarities between the sites. Oxygen atoms O-3, O-4, and O-5 of fucose are involved in hydrogen bonds with side chains of amino acids conserved in all repeats, whereas O-1 and O-2 interact with a large number of water molecules. The nonpolar face of each fucose residue is stacked against the aromatic ring of a Trp or Tyr amino acid, and the methyl group is located in a highly hydrophobic pocket. Depending on the precise binding site geometry, the alpha- or beta-anomer of the fucose ligand is observed bound in the crystal. Surface plasmon resonance experiments conducted on a series of oligosaccharides confirm the broad specificity of the lectin, with a slight preference for alphaFuc1-2Gal disaccharide. This multivalent carbohydrate recognition fold is a new prototype of lectins that is proposed to be involved in the host recognition strategy of several pathogenic organisms including not only the fungi Aspergillus but also the phytopathogenic bacterium Ralstonia solanacearum.
		+
		+	Crystal structure of fungal lectin: six-bladed beta-propeller fold and novel fucose recognition mode for Aleuria aurantia lectin.,Wimmerova M, Mitchell E, Sanchez JF, Gautier C, Imberty A J Biol Chem. 2003 Jul 18;278(29):27059-67. Epub 2003 May 5. PMID:12732625<ref>PMID:12732625</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1ofz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 05:47, 17 April 2024

OCA — Wed, 17 Apr 2024 05:47:06 GMT

←Older revision		Revision as of 05:47, 17 April 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [https://pdbe.org/1ofz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [https://www.ebi.ac.uk/pdbsum/1ofz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ofz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [https://pdbe.org/1ofz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [https://www.ebi.ac.uk/pdbsum/1ofz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ofz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LECF_ALEAU LECF_ALEAU]] Might have a role in the differentiation of cells. Binds L-fucose.	+	[https://www.uniprot.org/uniprot/LECF_ALEAU LECF_ALEAU] Might have a role in the differentiation of cells. Binds L-fucose.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ofz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ofz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Aleuria aurantia lectin is a fungal protein composed of two identical 312-amino acid subunits that specifically recognizes fucosylated glycans. The crystal structure of the lectin complexed with fucose reveals that each monomer consists of a six-bladed beta-propeller fold and of a small antiparallel two-stranded beta-sheet that plays a role in dimerization. Five fucose residues were located in binding pockets between the adjacent propeller blades. Due to repeats in the amino acid sequence, there are strong similarities between the sites. Oxygen atoms O-3, O-4, and O-5 of fucose are involved in hydrogen bonds with side chains of amino acids conserved in all repeats, whereas O-1 and O-2 interact with a large number of water molecules. The nonpolar face of each fucose residue is stacked against the aromatic ring of a Trp or Tyr amino acid, and the methyl group is located in a highly hydrophobic pocket. Depending on the precise binding site geometry, the alpha- or beta-anomer of the fucose ligand is observed bound in the crystal. Surface plasmon resonance experiments conducted on a series of oligosaccharides confirm the broad specificity of the lectin, with a slight preference for alphaFuc1-2Gal disaccharide. This multivalent carbohydrate recognition fold is a new prototype of lectins that is proposed to be involved in the host recognition strategy of several pathogenic organisms including not only the fungi Aspergillus but also the phytopathogenic bacterium Ralstonia solanacearum.
-
-	Crystal structure of fungal lectin: six-bladed beta-propeller fold and novel fucose recognition mode for Aleuria aurantia lectin.,Wimmerova M, Mitchell E, Sanchez JF, Gautier C, Imberty A J Biol Chem. 2003 Jul 18;278(29):27059-67. Epub 2003 May 5. PMID:12732625<ref>PMID:12732625</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ofz" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Aleuria aurantia]]		[[Category: Aleuria aurantia]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gautier, C]]	+	[[Category: Gautier C]]
-	[[Category: Imberty, A]]	+	[[Category: Imberty A]]
-	[[Category: Mitchell, E]]	+	[[Category: Mitchell E]]
-	[[Category: Sanchez~~, J F~~]]	+	[[Category: Sanchez JF]]
-	[[Category: Wimmerova, M]]	+	[[Category: Wimmerova M]]
-	~~[[Category: Aal]]~~	+
-	~~[[Category: Aleuria aurantia lectin]]~~	+
-	~~[[Category: Fucose]]~~	+
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: Sugar binding protein]]~~	+
-	~~[[Category: Sugar-binding protein~~]]	+

OCA at 07:12, 25 August 2021

OCA — Wed, 25 Aug 2021 07:12:47 GMT

←Older revision		Revision as of 07:12, 25 August 2021
Line 3:		Line 3:
	<StructureSection load='1ofz' size='340' side='right'caption='[[1ofz]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1ofz' size='340' side='right'caption='[[1ofz]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [~~http~~://pdbe.org/1ofz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ofz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ofz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [https://pdbe.org/1ofz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [https://www.ebi.ac.uk/pdbsum/1ofz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ofz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LECF_ALEAU LECF_ALEAU]] Might have a role in the differentiation of cells. Binds L-fucose.	+	[[https://www.uniprot.org/uniprot/LECF_ALEAU LECF_ALEAU]] Might have a role in the differentiation of cells. Binds L-fucose.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:29, 20 November 2019

OCA — Wed, 20 Nov 2019 18:29:47 GMT

←Older revision		Revision as of 18:29, 20 November 2019
Line 1:		Line 1:

	==Crystal structure of fungal lectin : six-bladed beta-propeller fold and novel fucose recognition mode for aleuria aurantia lectin==		==Crystal structure of fungal lectin : six-bladed beta-propeller fold and novel fucose recognition mode for aleuria aurantia lectin==
-	<StructureSection load='1ofz' size='340' side='right' caption='[[1ofz]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='1ofz' size='340' side='right'caption='[[1ofz]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Aleuria aurantia]]		[[Category: Aleuria aurantia]]
		+	[[Category: Large Structures]]
	[[Category: Gautier, C]]		[[Category: Gautier, C]]
	[[Category: Imberty, A]]		[[Category: Imberty, A]]

OCA at 07:16, 7 February 2018

OCA — Wed, 07 Feb 2018 07:16:53 GMT

←Older revision		Revision as of 07:16, 7 February 2018
Line 1:		Line 1:
-	==~~CRYSTAL STRUCTURE OF FUNGAL LECTIN~~: ~~SIX~~-~~BLADED BETA~~-~~PROPELLER FOLD AND NOVEL FUCOSE RECOGNITION MODE FOR ALEURIA AURANTIA LECTIN~~==	+
		+	==Crystal structure of fungal lectin : six-bladed beta-propeller fold and novel fucose recognition mode for aleuria aurantia lectin==
	<StructureSection load='1ofz' size='340' side='right' caption='[[1ofz]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1ofz' size='340' side='right' caption='[[1ofz]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [http://pdbe.org/1ofz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [http://www.ebi.ac.uk/pdbsum/1ofz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [http://pdbe.org/1ofz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [http://www.ebi.ac.uk/pdbsum/1ofz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ofz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/1ofz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/1ofz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 41:		Line 42:
	[[Category: Fucose]]		[[Category: Fucose]]
	[[Category: Lectin]]		[[Category: Lectin]]
		+	[[Category: Sugar binding protein]]
	[[Category: Sugar-binding protein]]		[[Category: Sugar-binding protein]]

OCA at 06:04, 8 February 2016

OCA — Mon, 08 Feb 2016 06:04:11 GMT

←Older revision		Revision as of 06:04, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ofz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:37, 10 September 2015

OCA — Thu, 10 Sep 2015 08:37:08 GMT

←Older revision		Revision as of 08:37, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [http://www.ebi.ac.uk/pdbsum/1ofz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [http://pdbe.org/1ofz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [http://www.ebi.ac.uk/pdbsum/1ofz PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ofz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:10, 24 December 2014

OCA — Wed, 24 Dec 2014 21:10:24 GMT

←Older revision		Revision as of 21:10, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [http://www.ebi.ac.uk/pdbsum/1ofz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [http://www.ebi.ac.uk/pdbsum/1ofz PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LECF_ALEAU LECF_ALEAU]] Might have a role in the differentiation of cells. Binds L-fucose.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Aleuria aurantia]]		[[Category: Aleuria aurantia]]
-	[[Category: Gautier, C.]]	+	[[Category: Gautier, C]]
-	[[Category: Imberty, A.]]	+	[[Category: Imberty, A]]
-	[[Category: Mitchell, E.]]	+	[[Category: Mitchell, E]]
-	[[Category: Sanchez, J F.]]	+	[[Category: Sanchez, J F]]
-	[[Category: Wimmerova, M.]]	+	[[Category: Wimmerova, M]]
	[[Category: Aal]]		[[Category: Aal]]
	[[Category: Aleuria aurantia lectin]]		[[Category: Aleuria aurantia lectin]]

OCA at 16:54, 28 September 2014

OCA — Sun, 28 Sep 2014 16:54:37 GMT

←Older revision		Revision as of 16:54, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1ofz.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF FUNGAL LECTIN: SIX-BLADED BETA-PROPELLER FOLD AND NOVEL FUCOSE RECOGNITION MODE FOR ALEURIA AURANTIA LECTIN==
		+	<StructureSection load='1ofz' size='340' side='right' caption='[[1ofz]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ofz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OFZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ofz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ofz RCSB], [http://www.ebi.ac.uk/pdbsum/1ofz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/1ofz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aleuria aurantia lectin is a fungal protein composed of two identical 312-amino acid subunits that specifically recognizes fucosylated glycans. The crystal structure of the lectin complexed with fucose reveals that each monomer consists of a six-bladed beta-propeller fold and of a small antiparallel two-stranded beta-sheet that plays a role in dimerization. Five fucose residues were located in binding pockets between the adjacent propeller blades. Due to repeats in the amino acid sequence, there are strong similarities between the sites. Oxygen atoms O-3, O-4, and O-5 of fucose are involved in hydrogen bonds with side chains of amino acids conserved in all repeats, whereas O-1 and O-2 interact with a large number of water molecules. The nonpolar face of each fucose residue is stacked against the aromatic ring of a Trp or Tyr amino acid, and the methyl group is located in a highly hydrophobic pocket. Depending on the precise binding site geometry, the alpha- or beta-anomer of the fucose ligand is observed bound in the crystal. Surface plasmon resonance experiments conducted on a series of oligosaccharides confirm the broad specificity of the lectin, with a slight preference for alphaFuc1-2Gal disaccharide. This multivalent carbohydrate recognition fold is a new prototype of lectins that is proposed to be involved in the host recognition strategy of several pathogenic organisms including not only the fungi Aspergillus but also the phytopathogenic bacterium Ralstonia solanacearum.

-	~~{{STRUCTURE_1ofz\| PDB=1ofz \| SCENE= }}~~	+	Crystal structure of fungal lectin: six-bladed beta-propeller fold and novel fucose recognition mode for Aleuria aurantia lectin.,Wimmerova M, Mitchell E, Sanchez JF, Gautier C, Imberty A J Biol Chem. 2003 Jul 18;278(29):27059-67. Epub 2003 May 5. PMID:12732625<ref>PMID:12732625</ref>

-	~~===CRYSTAL STRUCTURE OF FUNGAL LECTIN: SIX-BLADED BETA-PROPELLER FOLD AND NOVEL FUCOSE RECOGNITION MODE FOR ALEURIA AURANTIA LECTIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12732625}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ofz]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aleuria_aurantia Aleuria aurantia]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012732625<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Aleuria aurantia]]		[[Category: Aleuria aurantia]]
	[[Category: Gautier, C.]]		[[Category: Gautier, C.]]

OCA: Protected "1ofz" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2012 22:12:27 GMT

Protected "1ofz" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:12, 12 December 2012