1ohc - Revision history

OCA at 09:00, 9 May 2024

OCA — Thu, 09 May 2024 09:00:14 GMT

←Older revision		Revision as of 09:00, 9 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohc ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Cdc14 family of dual-specificity protein phosphatases (DSPs) is conserved within eukaryotes and functions to down-regulate mitotic Cdk activities, promoting cytokinesis and mitotic exit. We have integrated structural and kinetic analyses to define the molecular mechanism of the dephosphorylation reaction catalysed by Cdc14. The structure of Cdc14 illustrates a novel arrangement of two domains, each with a DSP-like fold, arranged in tandem. The C-terminal domain contains the conserved PTP motif of the catalytic site, whereas the N-terminal domain, which shares no sequence similarity with other DSPs, contributes to substrate specificity, and lacks catalytic activity. The catalytic site is located at the base of a pronounced surface channel formed by the interface of the two domains, and regions of both domains interact with the phosphopeptide substrate. Specificity for a pSer-Pro motif is mediated by a hydrophobic pocket that is capable of accommodating the apolar Pro(P+1) residue of the peptide. Our structural and kinetic data support a role for Cdc14 in the preferential dephosphorylation of proteins modified by proline-directed kinases.
		+
		+	The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase.,Gray CH, Good VM, Tonks NK, Barford D EMBO J. 2003 Jul 15;22(14):3524-35. PMID:12853468<ref>PMID:12853468</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1ohc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 05:47, 17 April 2024

OCA — Wed, 17 Apr 2024 05:47:30 GMT

←Older revision		Revision as of 05:47, 17 April 2024
Line 3:		Line 3:
	<StructureSection load='1ohc' size='340' side='right'caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1ohc' size='340' side='right'caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHC FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1ohd\|1ohd]]~~, [[~~1ohe~~\|~~1ohe~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [https://pdbe.org/1ohc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [https://www.ebi.ac.uk/pdbsum/1ohc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [https://pdbe.org/1ohc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [https://www.ebi.ac.uk/pdbsum/1ohc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CC14B_HUMAN CC14B_HUMAN]] Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.<ref>PMID:9367992</ref> <ref>PMID:18662541</ref>	+	[https://www.uniprot.org/uniprot/CC14B_HUMAN CC14B_HUMAN] Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.<ref>PMID:9367992</ref> <ref>PMID:18662541</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohc ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Cdc14 family of dual-specificity protein phosphatases (DSPs) is conserved within eukaryotes and functions to down-regulate mitotic Cdk activities, promoting cytokinesis and mitotic exit. We have integrated structural and kinetic analyses to define the molecular mechanism of the dephosphorylation reaction catalysed by Cdc14. The structure of Cdc14 illustrates a novel arrangement of two domains, each with a DSP-like fold, arranged in tandem. The C-terminal domain contains the conserved PTP motif of the catalytic site, whereas the N-terminal domain, which shares no sequence similarity with other DSPs, contributes to substrate specificity, and lacks catalytic activity. The catalytic site is located at the base of a pronounced surface channel formed by the interface of the two domains, and regions of both domains interact with the phosphopeptide substrate. Specificity for a pSer-Pro motif is mediated by a hydrophobic pocket that is capable of accommodating the apolar Pro(P+1) residue of the peptide. Our structural and kinetic data support a role for Cdc14 in the preferential dephosphorylation of proteins modified by proline-directed kinases.
-
-	The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase.,Gray CH, Good VM, Tonks NK, Barford D EMBO J. 2003 Jul 15;22(14):3524-35. PMID:12853468<ref>PMID:12853468</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ohc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Barford, D]]	+	[[Category: Barford D]]
-	[[Category: Good~~, V M~~]]	+	[[Category: Good VM]]
-	[[Category: Gray~~, C H~~]]	+	[[Category: Gray CH]]
-	[[Category: Tonks~~, N K]]~~	+	[[Category: Tonks NK]]
-	~~[[Category: Dual specificity phosphatase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 10:27, 17 February 2021

OCA — Wed, 17 Feb 2021 10:27:14 GMT

←Older revision		Revision as of 10:27, 17 February 2021
Line 3:		Line 3:
	<StructureSection load='1ohc' size='340' side='right'caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1ohc' size='340' side='right'caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHC FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohd\|1ohd]], [[1ohe\|1ohe]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ohd\|1ohd]], [[1ohe\|1ohe]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [~~http~~://pdbe.org/1ohc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ohc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [https://pdbe.org/1ohc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [https://www.ebi.ac.uk/pdbsum/1ohc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CC14B_HUMAN CC14B_HUMAN]] Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.<ref>PMID:9367992</ref> <ref>PMID:18662541</ref>	+	[[https://www.uniprot.org/uniprot/CC14B_HUMAN CC14B_HUMAN]] Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.<ref>PMID:9367992</ref> <ref>PMID:18662541</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Dual specificity phosphatase\|Dual specificity phosphatase]]	+	*[[Dual specificity phosphatase 3D structures\|Dual specificity phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:40, 10 May 2019

OCA — Fri, 10 May 2019 11:40:28 GMT

←Older revision		Revision as of 11:40, 10 May 2019
Line 1:		Line 1:

	==Structure of the proline directed phosphatase cdc14==		==Structure of the proline directed phosphatase cdc14==
-	<StructureSection load='1ohc' size='340' side='right' caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='1ohc' size='340' side='right'caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHC FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohc_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1ohc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ohc" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dual specificity phosphatase\|Dual specificity phosphatase]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Good, V M]]		[[Category: Good, V M]]

OCA at 09:47, 1 November 2017

OCA — Wed, 01 Nov 2017 09:47:28 GMT

←Older revision		Revision as of 09:47, 1 November 2017
Line 1:		Line 1:
-	==~~STRUCTURE OF THE PROLINE DIRECTED PHOSPHATASE CDC14~~==	+
		+	==Structure of the proline directed phosphatase cdc14==
	<StructureSection load='1ohc' size='340' side='right' caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1ohc' size='340' side='right' caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHC FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohd\|1ohd]], [[1ohe\|1ohe]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohd\|1ohd]], [[1ohe\|1ohe]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [http://pdbe.org/1ohc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [http://www.ebi.ac.uk/pdbsum/1ohc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [http://pdbe.org/1ohc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [http://www.ebi.ac.uk/pdbsum/1ohc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1ohc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ohc" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Dual specificity phosphatase\|Dual specificity phosphatase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:50, 11 September 2015

OCA — Fri, 11 Sep 2015 10:50:43 GMT

←Older revision		Revision as of 10:50, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ohc' size='340' side='right' caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1ohc' size='340' side='right' caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHC FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohd\|1ohd]], [[1ohe\|1ohe]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohd\|1ohd]], [[1ohe\|1ohe]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [http://www.ebi.ac.uk/pdbsum/1ohc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [http://pdbe.org/1ohc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [http://www.ebi.ac.uk/pdbsum/1ohc PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ohc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Good, V M]]		[[Category: Good, V M]]

OCA at 15:27, 25 December 2014

OCA — Thu, 25 Dec 2014 15:27:20 GMT

←Older revision		Revision as of 15:27, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohd\|1ohd]], [[1ohe\|1ohe]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohd\|1ohd]], [[1ohe\|1ohe]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [http://www.ebi.ac.uk/pdbsum/1ohc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [http://www.ebi.ac.uk/pdbsum/1ohc PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CC14B_HUMAN CC14B_HUMAN]] Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.<ref>PMID:9367992</ref> <ref>PMID:18662541</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Barford, D.]]	+	[[Category: Barford, D]]
-	[[Category: Good, V M.]]	+	[[Category: Good, V M]]
-	[[Category: Gray, C H.]]	+	[[Category: Gray, C H]]
-	[[Category: Tonks, N K.]]	+	[[Category: Tonks, N K]]
	[[Category: Dual specificity phosphatase]]		[[Category: Dual specificity phosphatase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 13:22, 28 September 2014

OCA — Sun, 28 Sep 2014 13:22:56 GMT

←Older revision		Revision as of 13:22, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ohc.~~png~~\|~~left~~\|200px]]	+	==STRUCTURE OF THE PROLINE DIRECTED PHOSPHATASE CDC14==
		+	<StructureSection load='1ohc' size='340' side='right' caption='[[1ohc]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ohc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohd\|1ohd]], [[1ohe\|1ohe]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohc RCSB], [http://www.ebi.ac.uk/pdbsum/1ohc PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohc_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Cdc14 family of dual-specificity protein phosphatases (DSPs) is conserved within eukaryotes and functions to down-regulate mitotic Cdk activities, promoting cytokinesis and mitotic exit. We have integrated structural and kinetic analyses to define the molecular mechanism of the dephosphorylation reaction catalysed by Cdc14. The structure of Cdc14 illustrates a novel arrangement of two domains, each with a DSP-like fold, arranged in tandem. The C-terminal domain contains the conserved PTP motif of the catalytic site, whereas the N-terminal domain, which shares no sequence similarity with other DSPs, contributes to substrate specificity, and lacks catalytic activity. The catalytic site is located at the base of a pronounced surface channel formed by the interface of the two domains, and regions of both domains interact with the phosphopeptide substrate. Specificity for a pSer-Pro motif is mediated by a hydrophobic pocket that is capable of accommodating the apolar Pro(P+1) residue of the peptide. Our structural and kinetic data support a role for Cdc14 in the preferential dephosphorylation of proteins modified by proline-directed kinases.

-	~~{{STRUCTURE_1ohc\| PDB=1ohc \| SCENE= }}~~	+	The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase.,Gray CH, Good VM, Tonks NK, Barford D EMBO J. 2003 Jul 15;22(14):3524-35. PMID:12853468<ref>PMID:12853468</ref>

-	~~===STRUCTURE OF THE PROLINE DIRECTED PHOSPHATASE CDC14===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_12853468}}~~	+	==See Also==
-		+	*[[Dual specificity phosphatase\|Dual specificity phosphatase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1ohc~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:012853468<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Barford, D.]]		[[Category: Barford, D.]]

OCA: Protected "1ohc" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2012 21:46:02 GMT

Protected "1ohc" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:46, 12 December 2012

OCA at 21:45, 12 December 2012

OCA — Wed, 12 Dec 2012 21:45:59 GMT

←Older revision		Revision as of 21:45, 12 December 2012
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-	{{Seed}}
	[[Image:1ohc.png\|left\|200px]]		[[Image:1ohc.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1ohc\| PDB=1ohc \| SCENE= }}		{{STRUCTURE_1ohc\| PDB=1ohc \| SCENE= }}

	===STRUCTURE OF THE PROLINE DIRECTED PHOSPHATASE CDC14===		===STRUCTURE OF THE PROLINE DIRECTED PHOSPHATASE CDC14===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12853468}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12853468 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_12853468}}		{{ABSTRACT_PUBMED_12853468}}

	==About this Structure==		==About this Structure==
-	~~1OHC~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA].	+	[[1ohc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHC OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12853468~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012853468</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Barford, D.]]		[[Category: Barford, D.]]
-	[[Category: Good, V.]]	+	[[Category: Good, V M.]]
-	[[Category: Gray, C.]]	+	[[Category: Gray, C H.]]
-	[[Category: Tonks, N.]]	+	[[Category: Tonks, N K.]]
	[[Category: Dual specificity phosphatase]]		[[Category: Dual specificity phosphatase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:19:27 2009''