1ohd - Revision history

OCA at 09:00, 9 May 2024

OCA — Thu, 09 May 2024 09:00:16 GMT

←Older revision		Revision as of 09:00, 9 May 2024
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohd ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Cdc14 family of dual-specificity protein phosphatases (DSPs) is conserved within eukaryotes and functions to down-regulate mitotic Cdk activities, promoting cytokinesis and mitotic exit. We have integrated structural and kinetic analyses to define the molecular mechanism of the dephosphorylation reaction catalysed by Cdc14. The structure of Cdc14 illustrates a novel arrangement of two domains, each with a DSP-like fold, arranged in tandem. The C-terminal domain contains the conserved PTP motif of the catalytic site, whereas the N-terminal domain, which shares no sequence similarity with other DSPs, contributes to substrate specificity, and lacks catalytic activity. The catalytic site is located at the base of a pronounced surface channel formed by the interface of the two domains, and regions of both domains interact with the phosphopeptide substrate. Specificity for a pSer-Pro motif is mediated by a hydrophobic pocket that is capable of accommodating the apolar Pro(P+1) residue of the peptide. Our structural and kinetic data support a role for Cdc14 in the preferential dephosphorylation of proteins modified by proline-directed kinases.
		+
		+	The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase.,Gray CH, Good VM, Tonks NK, Barford D EMBO J. 2003 Jul 15;22(14):3524-35. PMID:12853468<ref>PMID:12853468</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1ohd" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 05:47, 17 April 2024

OCA — Wed, 17 Apr 2024 05:47:32 GMT

←Older revision		Revision as of 05:47, 17 April 2024
Line 3:		Line 3:
	<StructureSection load='1ohd' size='340' side='right'caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1ohd' size='340' side='right'caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHD FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1ohc\|1ohc]], [[1ohe\|1ohe]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [https://pdbe.org/1ohd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [https://www.ebi.ac.uk/pdbsum/1ohd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [https://pdbe.org/1ohd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [https://www.ebi.ac.uk/pdbsum/1ohd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CC14B_HUMAN CC14B_HUMAN]] Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.<ref>PMID:9367992</ref> <ref>PMID:18662541</ref>	+	[https://www.uniprot.org/uniprot/CC14B_HUMAN CC14B_HUMAN] Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.<ref>PMID:9367992</ref> <ref>PMID:18662541</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohd ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Cdc14 family of dual-specificity protein phosphatases (DSPs) is conserved within eukaryotes and functions to down-regulate mitotic Cdk activities, promoting cytokinesis and mitotic exit. We have integrated structural and kinetic analyses to define the molecular mechanism of the dephosphorylation reaction catalysed by Cdc14. The structure of Cdc14 illustrates a novel arrangement of two domains, each with a DSP-like fold, arranged in tandem. The C-terminal domain contains the conserved PTP motif of the catalytic site, whereas the N-terminal domain, which shares no sequence similarity with other DSPs, contributes to substrate specificity, and lacks catalytic activity. The catalytic site is located at the base of a pronounced surface channel formed by the interface of the two domains, and regions of both domains interact with the phosphopeptide substrate. Specificity for a pSer-Pro motif is mediated by a hydrophobic pocket that is capable of accommodating the apolar Pro(P+1) residue of the peptide. Our structural and kinetic data support a role for Cdc14 in the preferential dephosphorylation of proteins modified by proline-directed kinases.
-
-	The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase.,Gray CH, Good VM, Tonks NK, Barford D EMBO J. 2003 Jul 15;22(14):3524-35. PMID:12853468<ref>PMID:12853468</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ohd" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Barford, D]]	+	[[Category: Barford D]]
-	[[Category: Good~~, V M~~]]	+	[[Category: Good VM]]
-	[[Category: Gray~~, C H~~]]	+	[[Category: Gray CH]]
-	[[Category: Tonks~~, N K]]~~	+	[[Category: Tonks NK]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein phosphatase~~]]	+

OCA at 10:27, 17 February 2021

OCA — Wed, 17 Feb 2021 10:27:17 GMT

←Older revision		Revision as of 10:27, 17 February 2021
Line 3:		Line 3:
	<StructureSection load='1ohd' size='340' side='right'caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1ohd' size='340' side='right'caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OHD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohc\|1ohc]], [[1ohe\|1ohe]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ohc\|1ohc]], [[1ohe\|1ohe]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [~~http~~://pdbe.org/1ohd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ohd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [https://pdbe.org/1ohd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [https://www.ebi.ac.uk/pdbsum/1ohd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CC14B_HUMAN CC14B_HUMAN]] Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.<ref>PMID:9367992</ref> <ref>PMID:18662541</ref>	+	[[https://www.uniprot.org/uniprot/CC14B_HUMAN CC14B_HUMAN]] Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.<ref>PMID:9367992</ref> <ref>PMID:18662541</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Dual specificity phosphatase\|Dual specificity phosphatase]]	+	*[[Dual specificity phosphatase 3D structures\|Dual specificity phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:40, 10 May 2019

OCA — Fri, 10 May 2019 11:40:33 GMT

←Older revision		Revision as of 11:40, 10 May 2019
Line 1:		Line 1:

	==structure of cdc14 in complex with tungstate==		==structure of cdc14 in complex with tungstate==
-	<StructureSection load='1ohd' size='340' side='right' caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='1ohd' size='340' side='right'caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHD FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohd_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohd_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1ohd" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ohd" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dual specificity phosphatase\|Dual specificity phosphatase]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Good, V M]]		[[Category: Good, V M]]

OCA at 10:04, 1 November 2017

OCA — Wed, 01 Nov 2017 10:04:32 GMT

←Older revision		Revision as of 10:04, 1 November 2017
Line 1:		Line 1:
-	==~~STRUCTURE OF CDC14 IN COMPLEX WITH TUNGSTATE~~==	+
		+	==structure of cdc14 in complex with tungstate==
	<StructureSection load='1ohd' size='340' side='right' caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1ohd' size='340' side='right' caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohc\|1ohc]], [[1ohe\|1ohe]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohc\|1ohc]], [[1ohe\|1ohe]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [http://pdbe.org/1ohd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [http://www.ebi.ac.uk/pdbsum/1ohd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [http://pdbe.org/1ohd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [http://www.ebi.ac.uk/pdbsum/1ohd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1ohd" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ohd" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Dual specificity phosphatase\|Dual specificity phosphatase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:56, 11 September 2015

OCA — Fri, 11 Sep 2015 09:56:39 GMT

←Older revision		Revision as of 09:56, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ohd' size='340' side='right' caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1ohd' size='340' side='right' caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohc\|1ohc]], [[1ohe\|1ohe]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohc\|1ohc]], [[1ohe\|1ohe]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [http://www.ebi.ac.uk/pdbsum/1ohd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [http://pdbe.org/1ohd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [http://www.ebi.ac.uk/pdbsum/1ohd PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ohd" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Good, V M]]		[[Category: Good, V M]]

OCA at 14:49, 25 December 2014

OCA — Thu, 25 Dec 2014 14:49:24 GMT

←Older revision		Revision as of 14:49, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHD FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohc\|1ohc]], [[1ohe\|1ohe]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohc\|1ohc]], [[1ohe\|1ohe]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [http://www.ebi.ac.uk/pdbsum/1ohd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [http://www.ebi.ac.uk/pdbsum/1ohd PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CC14B_HUMAN CC14B_HUMAN]] Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.<ref>PMID:9367992</ref> <ref>PMID:18662541</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Barford, D.]]	+	[[Category: Barford, D]]
-	[[Category: Good, V M.]]	+	[[Category: Good, V M]]
-	[[Category: Gray, C H.]]	+	[[Category: Gray, C H]]
-	[[Category: Tonks, N K.]]	+	[[Category: Tonks, N K]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Protein phosphatase]]		[[Category: Protein phosphatase]]

OCA at 18:02, 28 September 2014

OCA — Sun, 28 Sep 2014 18:02:30 GMT

←Older revision		Revision as of 18:02, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ohd.~~png~~\|~~left~~\|200px]]	+	==STRUCTURE OF CDC14 IN COMPLEX WITH TUNGSTATE==
		+	<StructureSection load='1ohd' size='340' side='right' caption='[[1ohd]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ohd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHD FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ohc\|1ohc]], [[1ohe\|1ohe]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohd RCSB], [http://www.ebi.ac.uk/pdbsum/1ohd PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohd_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Cdc14 family of dual-specificity protein phosphatases (DSPs) is conserved within eukaryotes and functions to down-regulate mitotic Cdk activities, promoting cytokinesis and mitotic exit. We have integrated structural and kinetic analyses to define the molecular mechanism of the dephosphorylation reaction catalysed by Cdc14. The structure of Cdc14 illustrates a novel arrangement of two domains, each with a DSP-like fold, arranged in tandem. The C-terminal domain contains the conserved PTP motif of the catalytic site, whereas the N-terminal domain, which shares no sequence similarity with other DSPs, contributes to substrate specificity, and lacks catalytic activity. The catalytic site is located at the base of a pronounced surface channel formed by the interface of the two domains, and regions of both domains interact with the phosphopeptide substrate. Specificity for a pSer-Pro motif is mediated by a hydrophobic pocket that is capable of accommodating the apolar Pro(P+1) residue of the peptide. Our structural and kinetic data support a role for Cdc14 in the preferential dephosphorylation of proteins modified by proline-directed kinases.

-	~~{{STRUCTURE_1ohd\| PDB=1ohd \| SCENE= }}~~	+	The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase.,Gray CH, Good VM, Tonks NK, Barford D EMBO J. 2003 Jul 15;22(14):3524-35. PMID:12853468<ref>PMID:12853468</ref>

-	~~===STRUCTURE OF CDC14 IN COMPLEX WITH TUNGSTATE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_12853468}}~~	+	==See Also==
-		+	*[[Dual specificity phosphatase\|Dual specificity phosphatase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1ohd~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:012853468<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Barford, D.]]		[[Category: Barford, D.]]

OCA: Protected "1ohd" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2012 22:07:53 GMT

Protected "1ohd" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:07, 12 December 2012

OCA at 22:07, 12 December 2012

OCA — Wed, 12 Dec 2012 22:07:33 GMT

←Older revision		Revision as of 22:07, 12 December 2012
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-	{{Seed}}
	[[Image:1ohd.png\|left\|200px]]		[[Image:1ohd.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1ohd\| PDB=1ohd \| SCENE= }}		{{STRUCTURE_1ohd\| PDB=1ohd \| SCENE= }}

	===STRUCTURE OF CDC14 IN COMPLEX WITH TUNGSTATE===		===STRUCTURE OF CDC14 IN COMPLEX WITH TUNGSTATE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12853468}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12853468 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_12853468}}		{{ABSTRACT_PUBMED_12853468}}

	==About this Structure==		==About this Structure==
-	~~1OHD~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA].	+	[[1ohd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHD OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12853468~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012853468</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Barford, D.]]		[[Category: Barford, D.]]
-	[[Category: Good, V.]]	+	[[Category: Good, V M.]]
-	[[Category: Gray, C.]]	+	[[Category: Gray, C H.]]
-	[[Category: Tonks, N.]]	+	[[Category: Tonks, N K.]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Protein phosphatase]]		[[Category: Protein phosphatase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:07:38 2009''