1ohn - Revision history

OCA at 05:47, 17 April 2024

OCA — Wed, 17 Apr 2024 05:47:38 GMT

←Older revision		Revision as of 05:47, 17 April 2024
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	==Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P.==		==Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P.==
-	<StructureSection load='1ohn' size='340' side='right'caption='[[1ohn~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='1ohn' size='340' side='right'caption='[[1ohn]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_15521 Atcc 15521~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Latilactobacillus_sakei Latilactobacillus sakei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHN FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1og7\|1og7]], [[1ohm\|1ohm]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [https://pdbe.org/1ohn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [https://www.ebi.ac.uk/pdbsum/1ohn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [https://pdbe.org/1ohn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [https://www.ebi.ac.uk/pdbsum/1ohn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~SAKP_LACSK SAKP_LACSK]~~] Bactericidal activity; inhibits closely related Lactobacilli, Listeria monocytogenes and ivanovvi, Enterococcus faecalis, Carnobacterium sp and Brocothrix thermosphacta.	+	[https://www.uniprot.org/uniprot/SAKP_LATSK SAKP_LATSK] Bactericidal activity; inhibits closely related Lactobacilli, Listeria monocytogenes and ivanovvi, Enterococcus faecalis, Carnobacterium sp and Brocothrix thermosphacta.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohn ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The three-dimensional structures in dodecylphosphocholine (DPC) micelles and in trifluoroethanol (TFE) of the pediocin-like antimicrobial peptide sakacin P and an engineered variant of sakacin P (termed sakP[N24C+44C]) have been determined by use of nuclear magnetic resonance spectroscopy. SakP[N24C+44C] has an inserted non-native activity- and structure-stabilizing C-terminal disulfide bridge that ties the C-terminus to the middle part of the peptide. In the presence of DPC, the cationic N-terminal region (residues 1-17) of both peptides has an S-shaped conformation that is reminiscent of a three-stranded antiparallel beta-sheet and that is more pronounced when the peptide was dissolved in TFE instead of DPC. The four positively charged residues located in the N-terminal part are found pointing to the same direction. For both peptides, the N-terminal region is followed by a well-defined central amphiphilic alpha-helix (residues 18-33), and this in turn is followed by the C-terminal tail (residues 34-43 for sakacin P and 34-44 for sakP[N24C+44C]) that lacks any apparent common secondary structural motif. In the presence of DPC, the C-terminal tails in both peptides fold back onto the central alpha-helix, thereby creating a hairpin-like structure in the C-terminal halves. The lack of long-range NOEs between the beta-sheet Nu-terminal region and the hairpin-like C-terminal half indicates that there is a flexible hinge between these regions. We discuss which implications such a structural arrangement has on the interaction with the target cell membrane.
-
-	Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P and a sakacin P variant that is structurally stabilized by an inserted C-terminal disulfide bridge.,Uteng M, Hauge HH, Markwick PR, Fimland G, Mantzilas D, Nissen-Meyer J, Muhle-Goll C Biochemistry. 2003 Oct 7;42(39):11417-26. PMID:14516192<ref>PMID:14516192</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ohn" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 15521]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fimland, G]]	+	[[Category: Latilactobacillus sakei]]
-	[[Category: Hauge~~, H H~~]]	+	[[Category: Fimland G]]
-	[[Category: Mantzilas, D]]	+	[[Category: Hauge HH]]
-	[[Category: Markwick~~, P R~~]]	+	[[Category: Mantzilas D]]
-	[[Category: Muhle-Goll, C]]	+	[[Category: Markwick PR]]
-	[[Category: Nissen-Meyer, J]]	+	[[Category: Muhle-Goll C]]
-	[[Category: Uteng, M]]	+	[[Category: Nissen-Meyer J]]
-	~~[[Category: Antibiotic]]~~	+	[[Category: Uteng M]]
-	~~[[Category: Antimicrobial peptide]]~~	+
-	~~[[Category: Bacteriocin]]~~	+
-	~~[[Category: Pediocin-like bacteriocin]]~~	+
-	~~[[Category: Sakacin antibiotic~~]]	+

OCA at 07:13, 25 August 2021

OCA — Wed, 25 Aug 2021 07:13:16 GMT

←Older revision		Revision as of 07:13, 25 August 2021
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	<StructureSection load='1ohn' size='340' side='right'caption='[[1ohn]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1ohn' size='340' side='right'caption='[[1ohn]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_15521 Atcc 15521]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OHN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_15521 Atcc 15521]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHN FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1og7\|1og7]], [[1ohm\|1ohm]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1og7\|1og7]], [[1ohm\|1ohm]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [~~http~~://pdbe.org/1ohn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ohn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [https://pdbe.org/1ohn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [https://www.ebi.ac.uk/pdbsum/1ohn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SAKP_LACSK SAKP_LACSK]] Bactericidal activity; inhibits closely related Lactobacilli, Listeria monocytogenes and ivanovvi, Enterococcus faecalis, Carnobacterium sp and Brocothrix thermosphacta.	+	[[https://www.uniprot.org/uniprot/SAKP_LACSK SAKP_LACSK]] Bactericidal activity; inhibits closely related Lactobacilli, Listeria monocytogenes and ivanovvi, Enterococcus faecalis, Carnobacterium sp and Brocothrix thermosphacta.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:16, 20 November 2019

OCA — Wed, 20 Nov 2019 18:16:27 GMT

←Older revision		Revision as of 18:16, 20 November 2019
Line 1:		Line 1:

	==Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P.==		==Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P.==
-	<StructureSection load='1ohn' size='340' side='right' caption='[[1ohn]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='1ohn' size='340' side='right'caption='[[1ohn]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_15521 Atcc 15521]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_15521 Atcc 15521]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHN FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 15521]]		[[Category: Atcc 15521]]
		+	[[Category: Large Structures]]
	[[Category: Fimland, G]]		[[Category: Fimland, G]]
	[[Category: Hauge, H H]]		[[Category: Hauge, H H]]

OCA at 07:05, 7 February 2018

OCA — Wed, 07 Feb 2018 07:05:36 GMT

←Older revision		Revision as of 07:05, 7 February 2018
Line 1:		Line 1:
-	==~~THREE~~-~~DIMENSIONAL STRUCTURE IN LIPID MICELLES OF THE PEDIOCIN~~-~~LIKE ANTIMICROBIAL PEPTIDE SAKACIN~~ P.==	+
		+	==Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P.==
	<StructureSection load='1ohn' size='340' side='right' caption='[[1ohn]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1ohn' size='340' side='right' caption='[[1ohn]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_15521 Atcc 15521]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_15521 Atcc 15521]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHN FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1og7\|1og7]], [[1ohm\|1ohm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1og7\|1og7]], [[1ohm\|1ohm]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [http://pdbe.org/1ohn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [http://www.ebi.ac.uk/pdbsum/1ohn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [http://pdbe.org/1ohn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [http://www.ebi.ac.uk/pdbsum/1ohn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 23:24, 9 February 2016

OCA — Tue, 09 Feb 2016 23:24:19 GMT

←Older revision		Revision as of 23:24, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:04, 11 September 2015

OCA — Fri, 11 Sep 2015 03:04:48 GMT

←Older revision		Revision as of 03:04, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ohn' size='340' side='right' caption='[[1ohn]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1ohn' size='340' side='right' caption='[[1ohn]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lactobacillus_sakei Lactobacillus sakei~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_15521 Atcc 15521]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHN FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1og7\|1og7]], [[1ohm\|1ohm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1og7\|1og7]], [[1ohm\|1ohm]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [http://www.ebi.ac.uk/pdbsum/1ohn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [http://pdbe.org/1ohn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [http://www.ebi.ac.uk/pdbsum/1ohn PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ohn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lactobacillus sakei~~]]	+	[[Category: Atcc 15521]]
	[[Category: Fimland, G]]		[[Category: Fimland, G]]
	[[Category: Hauge, H H]]		[[Category: Hauge, H H]]

OCA at 10:13, 25 December 2014

OCA — Thu, 25 Dec 2014 10:13:03 GMT

←Older revision		Revision as of 10:13, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_sakei Lactobacillus sakei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_sakei Lactobacillus sakei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1og7\|1og7]], [[1ohm\|1ohm]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1og7\|1og7]], [[1ohm\|1ohm]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [http://www.ebi.ac.uk/pdbsum/1ohn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [http://www.ebi.ac.uk/pdbsum/1ohn PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAKP_LACSK SAKP_LACSK]] Bactericidal activity; inhibits closely related Lactobacilli, Listeria monocytogenes and ivanovvi, Enterococcus faecalis, Carnobacterium sp and Brocothrix thermosphacta.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Lactobacillus sakei]]		[[Category: Lactobacillus sakei]]
-	[[Category: Fimland, G.]]	+	[[Category: Fimland, G]]
-	[[Category: Hauge, H H.]]	+	[[Category: Hauge, H H]]
-	[[Category: Mantzilas, D.]]	+	[[Category: Mantzilas, D]]
-	[[Category: Markwick, P R.]]	+	[[Category: Markwick, P R]]
-	[[Category: Muhle-Goll, C.]]	+	[[Category: Muhle-Goll, C]]
-	[[Category: Nissen-Meyer, J.]]	+	[[Category: Nissen-Meyer, J]]
-	[[Category: Uteng, M.]]	+	[[Category: Uteng, M]]
	[[Category: Antibiotic]]		[[Category: Antibiotic]]
	[[Category: Antimicrobial peptide]]		[[Category: Antimicrobial peptide]]

OCA at 16:35, 28 September 2014

OCA — Sun, 28 Sep 2014 16:35:09 GMT

←Older revision		Revision as of 16:35, 28 September 2014
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-	[[~~Image:~~1ohn.~~png~~\|~~left~~\|200px]]	+	==THREE-DIMENSIONAL STRUCTURE IN LIPID MICELLES OF THE PEDIOCIN-LIKE ANTIMICROBIAL PEPTIDE SAKACIN P.==
		+	<StructureSection load='1ohn' size='340' side='right' caption='[[1ohn]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ohn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_sakei Lactobacillus sakei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OHN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1og7\|1og7]], [[1ohm\|1ohm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ohn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ohn RCSB], [http://www.ebi.ac.uk/pdbsum/1ohn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The three-dimensional structures in dodecylphosphocholine (DPC) micelles and in trifluoroethanol (TFE) of the pediocin-like antimicrobial peptide sakacin P and an engineered variant of sakacin P (termed sakP[N24C+44C]) have been determined by use of nuclear magnetic resonance spectroscopy. SakP[N24C+44C] has an inserted non-native activity- and structure-stabilizing C-terminal disulfide bridge that ties the C-terminus to the middle part of the peptide. In the presence of DPC, the cationic N-terminal region (residues 1-17) of both peptides has an S-shaped conformation that is reminiscent of a three-stranded antiparallel beta-sheet and that is more pronounced when the peptide was dissolved in TFE instead of DPC. The four positively charged residues located in the N-terminal part are found pointing to the same direction. For both peptides, the N-terminal region is followed by a well-defined central amphiphilic alpha-helix (residues 18-33), and this in turn is followed by the C-terminal tail (residues 34-43 for sakacin P and 34-44 for sakP[N24C+44C]) that lacks any apparent common secondary structural motif. In the presence of DPC, the C-terminal tails in both peptides fold back onto the central alpha-helix, thereby creating a hairpin-like structure in the C-terminal halves. The lack of long-range NOEs between the beta-sheet Nu-terminal region and the hairpin-like C-terminal half indicates that there is a flexible hinge between these regions. We discuss which implications such a structural arrangement has on the interaction with the target cell membrane.

-	~~{{STRUCTURE_1ohn\| PDB=1ohn \| SCENE= }}~~	+	Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P and a sakacin P variant that is structurally stabilized by an inserted C-terminal disulfide bridge.,Uteng M, Hauge HH, Markwick PR, Fimland G, Mantzilas D, Nissen-Meyer J, Muhle-Goll C Biochemistry. 2003 Oct 7;42(39):11417-26. PMID:14516192<ref>PMID:14516192</ref>

-	~~===THREE-DIMENSIONAL STRUCTURE IN LIPID MICELLES OF THE PEDIOCIN-LIKE ANTIMICROBIAL PEPTIDE SAKACIN P.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14516192}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ohn]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lactobacillus_sakei Lactobacillus sakei]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014516192<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Lactobacillus sakei]]		[[Category: Lactobacillus sakei]]
	[[Category: Fimland, G.]]		[[Category: Fimland, G.]]

OCA: Protected "1ohn" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2012 21:49:51 GMT

Protected "1ohn" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:49, 12 December 2012

OCA at 21:49, 12 December 2012

OCA — Wed, 12 Dec 2012 21:49:49 GMT

←Older revision		Revision as of 21:49, 12 December 2012
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-	{{Seed}}
	[[Image:1ohn.png\|left\|200px]]		[[Image:1ohn.png\|left\|200px]]

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	{{STRUCTURE_1ohn\| PDB=1ohn \| SCENE= }}		{{STRUCTURE_1ohn\| PDB=1ohn \| SCENE= }}

	===THREE-DIMENSIONAL STRUCTURE IN LIPID MICELLES OF THE PEDIOCIN-LIKE ANTIMICROBIAL PEPTIDE SAKACIN P.===		===THREE-DIMENSIONAL STRUCTURE IN LIPID MICELLES OF THE PEDIOCIN-LIKE ANTIMICROBIAL PEPTIDE SAKACIN P.===

-
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	{{ABSTRACT_PUBMED_14516192}}		{{ABSTRACT_PUBMED_14516192}}

	==About this Structure==		==About this Structure==
-	~~1OHN~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_sakei Lactobacillus sakei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA].	+	[[1ohn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_sakei Lactobacillus sakei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHN OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14516192~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014516192</ref><references group="xtra"/>
	[[Category: Lactobacillus sakei]]		[[Category: Lactobacillus sakei]]
	[[Category: Fimland, G.]]		[[Category: Fimland, G.]]
	[[Category: Hauge, H H.]]		[[Category: Hauge, H H.]]
	[[Category: Mantzilas, D.]]		[[Category: Mantzilas, D.]]
-	[[Category: Markwick, P.]]	+	[[Category: Markwick, P R.]]
	[[Category: Muhle-Goll, C.]]		[[Category: Muhle-Goll, C.]]
	[[Category: Nissen-Meyer, J.]]		[[Category: Nissen-Meyer, J.]]
	[[Category: Uteng, M.]]		[[Category: Uteng, M.]]
		+	[[Category: Antibiotic]]
	[[Category: Antimicrobial peptide]]		[[Category: Antimicrobial peptide]]
	[[Category: Bacteriocin]]		[[Category: Bacteriocin]]
	[[Category: Pediocin-like bacteriocin]]		[[Category: Pediocin-like bacteriocin]]
	[[Category: Sakacin antibiotic]]		[[Category: Sakacin antibiotic]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:34:26 2009''