1ot3 - Revision history

OCA at 07:21, 25 October 2023

OCA — Wed, 25 Oct 2023 07:21:37 GMT

←Older revision		Revision as of 07:21, 25 October 2023
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	<StructureSection load='1ot3' size='340' side='right'caption='[[1ot3]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1ot3' size='340' side='right'caption='[[1ot3]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ot3]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OT3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ot3]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OT3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~, ~~<scene name='pdbligand=THM:THYMIDINE'>THM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase]~~, ~~with EC number [https~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [https://pdbe.org/1ot3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ot3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ot3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [https://pdbe.org/1ot3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ot3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ot3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DNK_DROME DNK_DROME]] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>	+	[https://www.uniprot.org/uniprot/DNK_DROME DNK_DROME] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</div>		</div>
	<div class="pdbe-citations 1ot3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ot3" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Thymidine kinase\|Thymidine kinase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Deoxynucleoside kinase]]~~	+	[[Category: Drosophila melanogaster]]
-	~~[[Category: Drome~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andersen, G]]	+	[[Category: Andersen G]]
-	[[Category: Eklund, H]]	+	[[Category: Eklund H]]
-	[[Category: Johansson, K]]	+	[[Category: Johansson K]]
-	[[Category: Karlsson, A]]	+	[[Category: Karlsson A]]
-	[[Category: Knecht, W]]	+	[[Category: Knecht W]]
-	[[Category: Mikkelsen~~, N E~~]]	+	[[Category: Mikkelsen NE]]
-	[[Category: Munch-Petersen, B]]	+	[[Category: Munch-Petersen B]]
-	[[Category: Piskur, J]]	+	[[Category: Piskur J]]
-	~~[[Category: Protein-deoxynucleoside complex]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:21, 8 September 2021

OCA — Wed, 08 Sep 2021 09:21:17 GMT

←Older revision		Revision as of 09:21, 8 September 2021
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	<StructureSection load='1ot3' size='340' side='right'caption='[[1ot3]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1ot3' size='340' side='right'caption='[[1ot3]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ot3]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OT3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ot3]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OT3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [~~http~~://pdbe.org/1ot3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ot3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ot3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [https://pdbe.org/1ot3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ot3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ot3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DNK_DROME DNK_DROME]] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>	+	[[https://www.uniprot.org/uniprot/DNK_DROME DNK_DROME]] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</div>		</div>
	<div class="pdbe-citations 1ot3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ot3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thymidine kinase\|Thymidine kinase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:50, 27 November 2019

OCA — Wed, 27 Nov 2019 09:50:34 GMT

←Older revision		Revision as of 09:50, 27 November 2019
Line 1:		Line 1:

	==Crystal structure of Drosophila deoxyribonucleotide kinase complexed with the substrate deoxythymidine==		==Crystal structure of Drosophila deoxyribonucleotide kinase complexed with the substrate deoxythymidine==
-	<StructureSection load='1ot3' size='340' side='right' caption='[[1ot3]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='1ot3' size='340' side='right'caption='[[1ot3]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ot3]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OT3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ot3]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OT3 FirstGlance]. <br>
Line 35:		Line 35:
	[[Category: Deoxynucleoside kinase]]		[[Category: Deoxynucleoside kinase]]
	[[Category: Drome]]		[[Category: Drome]]
		+	[[Category: Large Structures]]
	[[Category: Andersen, G]]		[[Category: Andersen, G]]
	[[Category: Eklund, H]]		[[Category: Eklund, H]]

OCA at 07:28, 15 February 2018

OCA — Thu, 15 Feb 2018 07:28:30 GMT

←Older revision		Revision as of 07:28, 15 February 2018
Line 1:		Line 1:
		+
	==Crystal structure of Drosophila deoxyribonucleotide kinase complexed with the substrate deoxythymidine==		==Crystal structure of Drosophila deoxyribonucleotide kinase complexed with the substrate deoxythymidine==
	<StructureSection load='1ot3' size='340' side='right' caption='[[1ot3]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1ot3' size='340' side='right' caption='[[1ot3]], [[Resolution\|resolution]] 2.50Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [http://pdbe.org/1ot3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ot3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [http://pdbe.org/1ot3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ot3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ot3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/1ot3_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/1ot3_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 10:45, 9 February 2016

OCA — Tue, 09 Feb 2016 10:45:31 GMT

←Older revision		Revision as of 10:45, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ot3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:31, 10 September 2015

OCA — Thu, 10 Sep 2015 21:31:40 GMT

←Older revision		Revision as of 21:31, 10 September 2015
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ot3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [http://pdbe.org/1ot3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ot3 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ot3" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:21, 25 December 2014

OCA — Thu, 25 Dec 2014 06:21:44 GMT

←Older revision		Revision as of 06:21, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ot3]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OT3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ot3]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OT3 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ot3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ot3 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DNK_DROME DNK_DROME]] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	[[Category: Deoxynucleoside kinase]]		[[Category: Deoxynucleoside kinase]]
	[[Category: Drome]]		[[Category: Drome]]
-	[[Category: Andersen, G.]]	+	[[Category: Andersen, G]]
-	[[Category: Eklund, H.]]	+	[[Category: Eklund, H]]
-	[[Category: Johansson, K.]]	+	[[Category: Johansson, K]]
-	[[Category: Karlsson, A.]]	+	[[Category: Karlsson, A]]
-	[[Category: Knecht, W.]]	+	[[Category: Knecht, W]]
-	[[Category: Mikkelsen, N E.]]	+	[[Category: Mikkelsen, N E]]
-	[[Category: Munch-Petersen, B.]]	+	[[Category: Munch-Petersen, B]]
-	[[Category: Piskur, J.]]	+	[[Category: Piskur, J]]
	[[Category: Protein-deoxynucleoside complex]]		[[Category: Protein-deoxynucleoside complex]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 11:32, 3 October 2014

OCA — Fri, 03 Oct 2014 11:32:02 GMT

←Older revision		Revision as of 11:32, 3 October 2014
Line 23:		Line 23:
	Structural basis for feedback inhibition of the deoxyribonucleoside salvage pathway: studies of the Drosophila deoxyribonucleoside kinase.,Mikkelsen NE, Johansson K, Karlsson A, Knecht W, Andersen G, Piskur J, Munch-Petersen B, Eklund H Biochemistry. 2003 May 20;42(19):5706-12. PMID:12741827<ref>PMID:12741827</ref>		Structural basis for feedback inhibition of the deoxyribonucleoside salvage pathway: studies of the Drosophila deoxyribonucleoside kinase.,Mikkelsen NE, Johansson K, Karlsson A, Knecht W, Andersen G, Piskur J, Munch-Petersen B, Eklund H Biochemistry. 2003 May 20;42(19):5706-12. PMID:12741827<ref>PMID:12741827</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 07:10, 28 May 2014

OCA — Wed, 28 May 2014 07:10:13 GMT

←Older revision		Revision as of 07:10, 28 May 2014
Line 1:		Line 1:
-	[[~~Image~~:1ot3.~~png\|left~~\|200px]]	+	==Crystal structure of Drosophila deoxyribonucleotide kinase complexed with the substrate deoxythymidine==
		+	<StructureSection load='1ot3' size='340' side='right' caption='[[1ot3]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ot3]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OT3 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ot3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ot3 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/1ot3_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Deoxyribonucleoside kinases are feedback inhibited by the final products of the salvage pathway, the deoxyribonucleoside triphosphates. In the present study, the mechanism of feedback inhibition is presented based on the crystal structure of a complex between the fruit fly deoxyribonucleoside kinase and its feedback inhibitor deoxythymidine triphosphate. The inhibitor was found to be bound as a bisubstrate inhibitor with its nucleoside part in the nucleoside binding site and with its phosphate groups partially occupying the phosphate donor site. The overall structure of the enzyme--inhibitor complex is very similar to the enzyme--substrate complexes with deoxythymidine and deoxycytidine, except for a conformational change within a region otherwise directly involved in catalysis. This conformational change involves a magnesium ion, which is coordinated in the inhibitor complex to the phosphates and to the primary base, Glu52, that normally is positioned close to the 5'-OH of the substrate deoxyribose.

-	~~{{STRUCTURE_1ot3\| PDB=1ot3 \| SCENE= }}~~	+	Structural basis for feedback inhibition of the deoxyribonucleoside salvage pathway: studies of the Drosophila deoxyribonucleoside kinase.,Mikkelsen NE, Johansson K, Karlsson A, Knecht W, Andersen G, Piskur J, Munch-Petersen B, Eklund H Biochemistry. 2003 May 20;42(19):5706-12. PMID:12741827<ref>PMID:12741827</ref>

-	~~===Crystal structure~~ of ~~Drosophila deoxyribonucleotide kinase complexed with~~ the ~~substrate deoxythymidine===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12741827}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ot3]] is a 8 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Drosophila_melanogaster Drosophila melanogaster]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1OT3 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012741827<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Deoxynucleoside kinase]]		[[Category: Deoxynucleoside kinase]]
-	[[Category: ~~Drosophila melanogaster~~]]	+	[[Category: Drome]]
	[[Category: Andersen, G.]]		[[Category: Andersen, G.]]
	[[Category: Eklund, H.]]		[[Category: Eklund, H.]]

OCA: Protected "1ot3" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2012 21:48:21 GMT

Protected "1ot3" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:48, 12 December 2012