1oxg - Revision history

OCA at 07:21, 25 October 2023

OCA — Wed, 25 Oct 2023 07:21:51 GMT

←Older revision		Revision as of 07:21, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oxg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OXG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oxg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OXG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1acb\|1acb]], [[4cha\|4cha]], [[6cha\|6cha]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [https://pdbe.org/1oxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [https://www.ebi.ac.uk/pdbsum/1oxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oxg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [https://pdbe.org/1oxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [https://www.ebi.ac.uk/pdbsum/1oxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oxg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CTRA_BOVIN CTRA_BOVIN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
-	[[Category: Chymotrypsin]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bilgrami, S]]	+	[[Category: Bilgrami S]]
-	[[Category: Gupta~~, M N~~]]	+	[[Category: Gupta MN]]
-	[[Category: Jabeen, T]]	+	[[Category: Jabeen T]]
-	[[Category: Roy, I]]	+	[[Category: Roy I]]
-	[[Category: Sharma, S]]	+	[[Category: Sharma S]]
-	[[Category: Singh, N]]	+	[[Category: Singh N]]
-	[[Category: Singh~~, T P]]~~	+	[[Category: Singh TP]]
-	~~[[Category: Autocatalysis]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Inhibition]]~~	+
-	~~[[Category: Organic solvent treatment~~]]	+

OCA at 09:22, 8 September 2021

OCA — Wed, 08 Sep 2021 09:22:37 GMT

←Older revision		Revision as of 09:22, 8 September 2021
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	<StructureSection load='1oxg' size='340' side='right'caption='[[1oxg]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1oxg' size='340' side='right'caption='[[1oxg]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1oxg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OXG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1oxg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OXG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acb\|1acb]], [[4cha\|4cha]], [[6cha\|6cha]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1acb\|1acb]], [[4cha\|4cha]], [[6cha\|6cha]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [~~http~~://pdbe.org/1oxg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1oxg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1oxg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [https://pdbe.org/1oxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [https://www.ebi.ac.uk/pdbsum/1oxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oxg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 10:10, 27 November 2019

OCA — Wed, 27 Nov 2019 10:10:36 GMT

←Older revision		Revision as of 10:10, 27 November 2019
Line 1:		Line 1:

	==Crystal structure of a complex formed between organic solvent treated bovine alpha-chymotrypsin and its autocatalytically produced highly potent 14-residue peptide at 2.2 resolution==		==Crystal structure of a complex formed between organic solvent treated bovine alpha-chymotrypsin and its autocatalytically produced highly potent 14-residue peptide at 2.2 resolution==
-	<StructureSection load='1oxg' size='340' side='right' caption='[[1oxg]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1oxg' size='340' side='right'caption='[[1oxg]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OXG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OXG FirstGlance]. <br>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1oxg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1oxg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chymotrypsin 3D structures\|Chymotrypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Chymotrypsin]]		[[Category: Chymotrypsin]]
		+	[[Category: Large Structures]]
	[[Category: Bilgrami, S]]		[[Category: Bilgrami, S]]
	[[Category: Gupta, M N]]		[[Category: Gupta, M N]]

OCA at 07:41, 15 February 2018

OCA — Thu, 15 Feb 2018 07:41:48 GMT

←Older revision		Revision as of 07:41, 15 February 2018
Line 1:		Line 1:
		+
	==Crystal structure of a complex formed between organic solvent treated bovine alpha-chymotrypsin and its autocatalytically produced highly potent 14-residue peptide at 2.2 resolution==		==Crystal structure of a complex formed between organic solvent treated bovine alpha-chymotrypsin and its autocatalytically produced highly potent 14-residue peptide at 2.2 resolution==
	<StructureSection load='1oxg' size='340' side='right' caption='[[1oxg]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1oxg' size='340' side='right' caption='[[1oxg]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acb\|1acb]], [[4cha\|4cha]], [[6cha\|6cha]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acb\|1acb]], [[4cha\|4cha]], [[6cha\|6cha]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [http://pdbe.org/1oxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [http://www.ebi.ac.uk/pdbsum/1oxg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [http://pdbe.org/1oxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [http://www.ebi.ac.uk/pdbsum/1oxg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1oxg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/1oxg_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/1oxg_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1oxg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1oxg" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Chymotrypsin\|Chymotrypsin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:24, 9 February 2016

OCA — Tue, 09 Feb 2016 13:24:36 GMT

←Older revision		Revision as of 13:24, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oxg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:39, 10 September 2015

OCA — Thu, 10 Sep 2015 22:39:36 GMT

←Older revision		Revision as of 22:39, 10 September 2015
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acb\|1acb]], [[4cha\|4cha]], [[6cha\|6cha]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acb\|1acb]], [[4cha\|4cha]], [[6cha\|6cha]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [http://www.ebi.ac.uk/pdbsum/1oxg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [http://pdbe.org/1oxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [http://www.ebi.ac.uk/pdbsum/1oxg PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1oxg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 07:06, 6 January 2015

OCA — Tue, 06 Jan 2015 07:06:28 GMT

←Older revision		Revision as of 07:06, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OXG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OXG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acb\|1acb]], [[4cha\|4cha]], [[6cha\|6cha]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acb\|1acb]], [[4cha\|4cha]], [[6cha\|6cha]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [http://www.ebi.ac.uk/pdbsum/1oxg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [http://www.ebi.ac.uk/pdbsum/1oxg PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Chymotrypsin]]		[[Category: Chymotrypsin]]
-	[[Category: Bilgrami, S.]]	+	[[Category: Bilgrami, S]]
-	[[Category: Gupta, M N.]]	+	[[Category: Gupta, M N]]
-	[[Category: Jabeen, T.]]	+	[[Category: Jabeen, T]]
-	[[Category: Roy, I.]]	+	[[Category: Roy, I]]
-	[[Category: Sharma, S.]]	+	[[Category: Sharma, S]]
-	[[Category: Singh, N.]]	+	[[Category: Singh, N]]
-	[[Category: Singh, T P.]]	+	[[Category: Singh, T P]]
	[[Category: Autocatalysis]]		[[Category: Autocatalysis]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Inhibition]]		[[Category: Inhibition]]
	[[Category: Organic solvent treatment]]		[[Category: Organic solvent treatment]]

OCA at 19:37, 28 September 2014

OCA — Sun, 28 Sep 2014 19:37:42 GMT

←Older revision		Revision as of 19:37, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1oxg.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of a complex formed between organic solvent treated bovine alpha-chymotrypsin and its autocatalytically produced highly potent 14-residue peptide at 2.2 resolution==
		+	<StructureSection load='1oxg' size='340' side='right' caption='[[1oxg]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1oxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OXG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1acb\|1acb]], [[4cha\|4cha]], [[6cha\|6cha]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oxg RCSB], [http://www.ebi.ac.uk/pdbsum/1oxg PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/1oxg_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chymotrypsin is a prominent member of the family of serine proteases. The present studies demonstrate the presence of a native fragment containing 14 residues from Ile16 to Trp29 in alpha-chymotrypsin that binds to chymotrypsin at the active site with an exceptionally high affinity of 2.7 +/- 0.3 x 10(-11) M and thus works as a highly potent competitive inhibitor. The commercially available alpha-chymotrypsin was processed through a three phase partitioning system (TPP). The treated enzyme showed considerably enhanced activity. The 14 residue fragment was produced by autodigestion of a TPP-treated alpha-chymotrypsin during a long crystallization process that lasted more than four months. The treated enzyme was purified and kept for crystallization using vapour the diffusion method at 295 K. Twenty milligrams of lyophilized protein were dissolved in 1 mL of 25 mM sodium acetate buffer, pH 4.8. It was equilibrated against the same buffer containing 1.2 M ammonium sulfate. The rectangular crystals of small dimensions of 0.24 x 0.15 x 0.10 mm(3) were obtained. The X-ray intensity data were collected at 2.2 angstroms resolution and the structure was refined to an R-factor of 0.192. An extra electron density was observed at the binding site of alpha-chymotrypsin, which was readily interpreted as a 14 residue fragment of alpha-chymotrypsin corresponding to Ile-Val-Asn-Gly-Glu-Glu-Ala-Val-Pro-Gly-Ser-Trp-Pro-Trp(16-29). The electron density for the eight residues of the C-terminus, i.e. Ala22-Trp29, which were completely buried in the binding cleft of the enzyme, was of excellent quality and all the side chains of these eight residues were clearly modeled into it. However, the remaining six residues from the N-terminus, Ile16-Glu21 were poorly defined although the backbone density was good. There was a continuous electron density at 3.0 sigma between the active site Ser195 Ogamma and the carbonyl carbon atom of Trp29 of the fragment. The final refined coordinates showed a distance of 1.35 angstroms between Ser195 Ogamma and Trp29 C indicating the presence of a covalent linkage between the enzyme and the native fragment. This meant that the enzyme formed an acyl intermediate with the autodigested fragment Ile16-Trp29. In addition to the O-C covalent bond, there were several hydrogen bonds and hydrophobic interactions between the enzyme and the native fragment. The fragment showed a high complementarity with the binding site of alpha-chymotrypsin and the buried part of the fragment matched excellently with the corresponding buried part of Turkey ovomucoid inhibitor of alpha-chymotrypsin.

-	~~{{STRUCTURE_1oxg\| PDB=1oxg \| SCENE= }}~~	+	Detection of native peptides as potent inhibitors of enzymes. Crystal structure of the complex formed between treated bovine alpha-chymotrypsin and an autocatalytically produced fragment, IIe-Val-Asn-Gly-Glu-Glu-Ala-Val-Pro-Gly-Ser-Trp-Pro-Trp, at 2.2 angstroms resolution.,Singh N, Jabeen T, Sharma S, Roy I, Gupta MN, Bilgrami S, Somvanshi RK, Dey S, Perbandt M, Betzel C, Srinivasan A, Singh TP FEBS J. 2005 Jan;272(2):562-72. PMID:15654893<ref>PMID:15654893</ref>

-	~~===Crystal structure~~ of ~~a complex formed between organic solvent treated bovine alpha-chymotrypsin and its autocatalytically produced highly potent 14-residue peptide at 2~~.~~2 resolution===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15654893}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1oxg]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA].~~	+

	==See Also==		==See Also==
	*[[Chymotrypsin\|Chymotrypsin]]		*[[Chymotrypsin\|Chymotrypsin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015654893<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Chymotrypsin]]		[[Category: Chymotrypsin]]

OCA at 13:58, 20 October 2012

OCA — Sat, 20 Oct 2012 13:58:59 GMT

←Older revision		Revision as of 13:58, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1oxg]] is a 2 chain structure ~~of [[Chymotrypsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA].	+	[[1oxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXG OCA].

	==See Also==		==See Also==

OCA at 13:42, 26 July 2012

OCA — Thu, 26 Jul 2012 13:42:09 GMT

←Older revision		Revision as of 13:42, 26 July 2012
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	[[Image:1oxg.png\|left\|200px]]		[[Image:1oxg.png\|left\|200px]]

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	===Crystal structure of a complex formed between organic solvent treated bovine alpha-chymotrypsin and its autocatalytically produced highly potent 14-residue peptide at 2.2 resolution===		===Crystal structure of a complex formed between organic solvent treated bovine alpha-chymotrypsin and its autocatalytically produced highly potent 14-residue peptide at 2.2 resolution===

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-	<!--
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	==See Also==		==See Also==
-	*[[Chymotrypsin]]	+	*[[Chymotrypsin\|Chymotrypsin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15654893~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015654893</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Chymotrypsin]]		[[Category: Chymotrypsin]]