1ozg - Revision history

OCA at 09:34, 16 August 2023

OCA — Wed, 16 Aug 2023 09:34:32 GMT

←Older revision		Revision as of 09:34, 16 August 2023
Line 3:		Line 3:
	<StructureSection load='1ozg' size='340' side='right'caption='[[1ozg]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1ozg' size='340' side='right'caption='[[1ozg]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ozg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus~~ pneumoniae~~" (schroeter 1886) flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1OZG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ozg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OZG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HE3:2-HYDROXYETHYL+DIHYDROTHIACHROME+DIPHOSPHATE'>HE3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ozf\|1ozf]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HE3:2-HYDROXYETHYL+DIHYDROTHIACHROME+DIPHOSPHATE'>HE3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [https://pdbe.org/1ozg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [https://www.ebi.ac.uk/pdbsum/1ozg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozg ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [~~http~~://pdbe.org/1ozg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ozg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozg ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ILVB_KLEPN ILVB_KLEPN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Klebsiella pneumoniae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lyase]]~~	+	[[Category: Duggleby RG]]
-	[[Category: Duggleby~~, R G~~]]	+	[[Category: Guddat LW]]
-	[[Category: Guddat~~, L W~~]]	+	[[Category: Pang SS]]
-	[[Category: Pang~~, S S~~]]	+	[[Category: Schowen RL]]
-	[[Category: Schowen~~, R L]]~~	+
-	~~[[Category: Acetohydroxyacid synthase]]~~	+
-	~~[[Category: Acetolactate synthase]]~~	+
-	~~[[Category: Thiamin diphosphate~~]]	+

OCA at 07:19, 9 December 2020

OCA — Wed, 09 Dec 2020 07:19:30 GMT

←Older revision		Revision as of 07:19, 9 December 2020
Line 1:		Line 1:

	==The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate==		==The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate==
-	<StructureSection load='1ozg' size='340' side='right' caption='[[1ozg]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='1ozg' size='340' side='right'caption='[[1ozg]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ozg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OZG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ozg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1OZG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HE3:2-HYDROXYETHYL+DIHYDROTHIACHROME+DIPHOSPHATE'>HE3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HE3:2-HYDROXYETHYL+DIHYDROTHIACHROME+DIPHOSPHATE'>HE3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ozf\|1ozf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ozf\|1ozf]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [http://pdbe.org/1ozg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [http://www.ebi.ac.uk/pdbsum/1ozg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [http://pdbe.org/1ozg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [http://www.ebi.ac.uk/pdbsum/1ozg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Duggleby, R G]]		[[Category: Duggleby, R G]]

OCA at 07:26, 15 February 2018

OCA — Thu, 15 Feb 2018 07:26:48 GMT

←Older revision		Revision as of 07:26, 15 February 2018
Line 1:		Line 1:
		+
	==The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate==		==The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate==
	<StructureSection load='1ozg' size='340' side='right' caption='[[1ozg]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1ozg' size='340' side='right' caption='[[1ozg]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [http://pdbe.org/1ozg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [http://www.ebi.ac.uk/pdbsum/1ozg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [http://pdbe.org/1ozg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [http://www.ebi.ac.uk/pdbsum/1ozg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1ozg_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1ozg_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ozg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:19, 11 September 2015

OCA — Fri, 11 Sep 2015 14:19:40 GMT

←Older revision		Revision as of 14:19, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ozg' size='340' side='right' caption='[[1ozg]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1ozg' size='340' side='right' caption='[[1ozg]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ozg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Klebsiella_pneumoniae Klebsiella~~ pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OZG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ozg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OZG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HE3:2-HYDROXYETHYL+DIHYDROTHIACHROME+DIPHOSPHATE'>HE3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HE3:2-HYDROXYETHYL+DIHYDROTHIACHROME+DIPHOSPHATE'>HE3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ozf\|1ozf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ozf\|1ozf]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 ~~Klebsiella~~ pneumoniae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Acetolactate_synthase Acetolactate synthase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.2~~.1.~~6 2~~.2.1.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [http://www.ebi.ac.uk/pdbsum/1ozg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [http://pdbe.org/1ozg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [http://www.ebi.ac.uk/pdbsum/1ozg PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ozg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Acetolactate synthase]]~~	+	[[Category: Lyase]]
-	~~[[Category: Klebsiella pneumoniae~~]]	+
	[[Category: Duggleby, R G]]		[[Category: Duggleby, R G]]
	[[Category: Guddat, L W]]		[[Category: Guddat, L W]]
Line 38:		Line 38:
	[[Category: Schowen, R L]]		[[Category: Schowen, R L]]
	[[Category: Acetohydroxyacid synthase]]		[[Category: Acetohydroxyacid synthase]]
-	[[Category: ~~Lyase~~]]	+	[[Category: Acetolactate synthase]]
	[[Category: Thiamin diphosphate]]		[[Category: Thiamin diphosphate]]

OCA at 07:42, 6 January 2015

OCA — Tue, 06 Jan 2015 07:42:42 GMT

←Older revision		Revision as of 07:42, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ozg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OZG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ozg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OZG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HE3:2-HYDROXYETHYL+DIHYDROTHIACHROME+DIPHOSPHATE'>HE3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HE3:2-HYDROXYETHYL+DIHYDROTHIACHROME+DIPHOSPHATE'>HE3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ozf\|1ozf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ozf\|1ozf]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [http://www.ebi.ac.uk/pdbsum/1ozg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [http://www.ebi.ac.uk/pdbsum/1ozg PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
-	[[Category: Duggleby, R G.]]	+	[[Category: Duggleby, R G]]
-	[[Category: Guddat, L W.]]	+	[[Category: Guddat, L W]]
-	[[Category: Pang, S S.]]	+	[[Category: Pang, S S]]
-	[[Category: Schowen, R L.]]	+	[[Category: Schowen, R L]]
	[[Category: Acetohydroxyacid synthase]]		[[Category: Acetohydroxyacid synthase]]
-	[[Category: Acetolactate synthase]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Thiamin diphosphate]]		[[Category: Thiamin diphosphate]]

OCA at 22:41, 28 September 2014

OCA — Sun, 28 Sep 2014 22:41:57 GMT

←Older revision		Revision as of 22:41, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ozg.~~png~~\|~~left~~\|200px]]	+	==The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate==
		+	<StructureSection load='1ozg' size='340' side='right' caption='[[1ozg]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ozg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OZG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HE3:2-HYDROXYETHYL+DIHYDROTHIACHROME+DIPHOSPHATE'>HE3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ozf\|1ozf]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ozg RCSB], [http://www.ebi.ac.uk/pdbsum/1ozg PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1ozg_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acetohydroxyacid synthase (AHAS) and acetolactate synthase (ALS) are thiamine diphosphate (ThDP)-dependent enzymes that catalyze the decarboxylation of pyruvate to give a cofactor-bound hydroxyethyl group, which is transferred to a second molecule of pyruvate to give 2-acetolactate. AHAS is found in plants, fungi, and bacteria, is involved in the biosynthesis of the branched-chain amino acids, and contains non-catalytic FAD. ALS is found only in some bacteria, is a catabolic enzyme required for the butanediol fermentation, and does not contain FAD. Here we report the 2.3-A crystal structure of Klebsiella pneumoniae ALS. The overall structure is similar to AHAS except for a groove that accommodates FAD in AHAS, which is filled with amino acid side chains in ALS. The ThDP cofactor has an unusual conformation that is unprecedented among the 26 known three-dimensional structures of nine ThDP-dependent enzymes, including AHAS. This conformation suggests a novel mechanism for ALS. A second structure, at 2.0 A, is described in which the enzyme is trapped halfway through the catalytic cycle so that it contains the hydroxyethyl intermediate bound to ThDP. The cofactor has a tricyclic structure that has not been observed previously in any ThDP-dependent enzyme, although similar structures are well known for free thiamine. This structure is consistent with our proposed mechanism and probably results from an intramolecular proton transfer within a tricyclic carbanion that is the true reaction intermediate. Modeling of the second molecule of pyruvate into the active site of the enzyme with the bound intermediate is consistent with the stereochemistry and specificity of ALS.

-	~~{{STRUCTURE_1ozg\| PDB=1ozg \| SCENE= }}~~	+	The crystal structures of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate.,Pang SS, Duggleby RG, Schowen RL, Guddat LW J Biol Chem. 2004 Jan 16;279(3):2242-53. Epub 2003 Oct 13. PMID:14557277<ref>PMID:14557277</ref>

-	~~===The crystal structure~~ of ~~Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14557277}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ozg]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014557277<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]

OCA: Protected "1ozg" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2012 21:45:30 GMT

Protected "1ozg" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:45, 12 December 2012

OCA at 21:45, 12 December 2012

OCA — Wed, 12 Dec 2012 21:45:27 GMT

←Older revision		Revision as of 21:45, 12 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1ozg.png\|left\|200px]]		[[Image:1ozg.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ozg", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ozg\| PDB=1ozg \| SCENE= }}		{{STRUCTURE_1ozg\| PDB=1ozg \| SCENE= }}

	===The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate===		===The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_14557277}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 14557277 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_14557277}}		{{ABSTRACT_PUBMED_14557277}}

	==About this Structure==		==About this Structure==
-	~~1OZG~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA].	+	[[1ozg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14557277~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014557277</ref><references group="xtra"/>
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
Line 32:		Line 20:
	[[Category: Acetohydroxyacid synthase]]		[[Category: Acetohydroxyacid synthase]]
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
		+	[[Category: Lyase]]
	[[Category: Thiamin diphosphate]]		[[Category: Thiamin diphosphate]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:24:50 2009''

OCA at 22:24, 17 February 2009

OCA — Tue, 17 Feb 2009 22:24:51 GMT

←Older revision		Revision as of 22:24, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1OZG is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA].	+	1OZG is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZG OCA].

	==Reference==		==Reference==
-	The crystal structures of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate., Pang SS, Duggleby RG, Schowen RL, Guddat LW, J Biol Chem. 2004 Jan 16;279(3):2242-53. Epub 2003 Oct 13. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14557277 14557277]~~	+	<ref group="xtra">PMID:14557277</ref><references group="xtra"/>
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
-	[[Category: Single protein]]
	[[Category: Duggleby, R G.]]		[[Category: Duggleby, R G.]]
	[[Category: Guddat, L W.]]		[[Category: Guddat, L W.]]
Line 35:		Line 34:
	[[Category: Thiamin diphosphate]]		[[Category: Thiamin diphosphate]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 07~~:47:~~23 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:24:50 2009''

OCA at 04:47, 29 July 2008

OCA — Tue, 29 Jul 2008 04:47:27 GMT

←Older revision		Revision as of 04:47, 29 July 2008
Line 1:		Line 1:
-	[[Image:1ozg.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1ozg.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1ozg\| PDB=1ozg \| SCENE= }}		{{STRUCTURE_1ozg\| PDB=1ozg \| SCENE= }}

-	~~'''~~The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate~~'''~~	+	===The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate===


-	~~==Overview==~~	+	<!--
-	~~Acetohydroxyacid synthase (AHAS) and acetolactate synthase (ALS) are thiamine diphosphate (ThDP)~~-~~dependent enzymes that catalyze the decarboxylation of pyruvate to give a cofactor~~-~~bound hydroxyethyl group~~, ~~which is transferred to a second molecule of pyruvate to give 2-acetolactate. AHAS is found in plants~~, ~~fungi, and bacteria, is involved in~~ the biosynthesis of the branched-chain amino acids, and contains non-catalytic FAD. ALS is found only in some bacteria, is a catabolic enzyme required for the butanediol fermentation, and does not contain FAD. Here we report the 2.3-A crystal structure of Klebsiella pneumoniae ALS. The overall structure is similar to ~~AHAS except for a groove that accommodates FAD in AHAS, which is filled with amino acid side chains in ALS. The ThDP cofactor has an unusual conformation that is unprecedented among~~ the ~~26 known three-dimensional structures of nine ThDP-dependent enzymes, including AHAS~~. ~~This conformation suggests a novel mechanism for ALS~~. ~~A second structure, at 2.0 A~~, is ~~described in which~~ the ~~enzyme is trapped halfway through the catalytic cycle so that it contains the hydroxyethyl intermediate bound to ThDP~~. ~~The cofactor has a tricyclic structure that has not been observed previously in any ThDP~~-dependent enzyme, although similar structures are well known for free thiamine. This structure is consistent with our proposed mechanism and probably results from an intramolecular proton transfer within a tricyclic carbanion that is the true reaction intermediate. Modeling of the second molecule of pyruvate into the active site of the enzyme with the bound intermediate is consistent with the stereochemistry and specificity of ALS.	+	The line below this paragraph, {{ABSTRACT_PUBMED_14557277}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 14557277 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_14557277}}

	==About this Structure==		==About this Structure==
Line 30:		Line 34:
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Thiamin diphosphate]]		[[Category: Thiamin diphosphate]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 04~~:28:22 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:47:23 2008''