1p9w - Revision history

OCA at 05:53, 17 April 2024

OCA — Wed, 17 Apr 2024 05:53:56 GMT

←Older revision		Revision as of 05:53, 17 April 2024
Line 3:		Line 3:
	<StructureSection load='1p9w' size='340' side='right'caption='[[1p9w]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1p9w' size='340' side='right'caption='[[1p9w]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1p9w]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1P9W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1p9w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P9W FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID~~-~~ADENYLATE+ESTER'>ANP</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[1p9r\|1p9r]]~~</~~div~~><~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [https://pdbe.org/1p9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [https://www.ebi.ac.uk/pdbsum/1p9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9w ProSAT]</span></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [~~http~~://pdbe.org/1p9w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1p9w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9w ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GSPE_VIBCH GSPE_VIBCH]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.	+	[https://www.uniprot.org/uniprot/GSPE_VIBCH GSPE_VIBCH] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 22:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p9w ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p9w ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Type II secretion systems consist of an assembly of 12-15 Gsp proteins responsible for transporting a variety of virulence factors across the outer membrane in several pathogenic bacteria. In Vibrio cholerae, the major virulence factor cholera toxin is secreted by the Eps Type II secretion apparatus consisting of 14 Eps proteins. One of these, EpsE, is a cytoplasmic putative NTPase essential for the functioning of the Eps system and member of the GspE subfamily of Type II secretion ATPases. The crystal structure of a truncated form of EpsE in nucleotide-liganded and unliganded state has been determined, and reveals a two-domain architecture with the four characteristic sequence "boxes" of the GspE subfamily clustering around the nucleotide-binding site of the C-domain. This domain contains two C-terminal subdomains not reported before in this superfamily of NTPases. One of these subdomains contains a four-cysteine motif that appears to be involved in metal binding as revealed by anomalous difference density. The EpsE subunits form a right-handed helical arrangement in the crystal with extensive and conserved contacts between the C and N domains of neighboring subunits. Combining the most conserved interface with the quaternary structure of the C domain in a distant homolog, a hexameric model for EpsE is proposed which may reflect the assembly of this critical protein in the Type II secretion system. The nucleotide ligand contacts both domains in this model. The N2-domain-containing surface of the hexamer appears to be highly conserved in the GspE family and most likely faces the inner membrane interacting with other members of the Eps system.
-
-	Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae.,Robien MA, Krumm BE, Sandkvist M, Hol WG J Mol Biol. 2003 Oct 24;333(3):657-74. PMID:14556751<ref>PMID:14556751</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1p9w" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[General secretion pathway protein 3D structures\|General secretion pathway protein 3D structures]]		*[[General secretion pathway protein 3D structures\|General secretion pathway protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Hol, W G.J~~]]	+	[[Category: Vibrio cholerae]]
-	[[Category: ~~Krumm, B E~~]]	+	[[Category: Hol WGJ]]
-	[[Category: ~~Robien, M A~~]]	+	[[Category: Krumm BE]]
-	[[Category: ~~Sandkvist, M~~]]	+	[[Category: Robien MA]]
-	[[Category: ~~Atp binding protein]]~~	+	[[Category: Sandkvist M]]
-	~~[[Category: Bacterial type ii secretion system cytoplasmic protein - gspe]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Putative atpase~~]]	+

OCA at 07:26, 9 December 2020

OCA — Wed, 09 Dec 2020 07:26:53 GMT

←Older revision		Revision as of 07:26, 9 December 2020
Line 1:		Line 1:

	==Crystal Structure of Vibrio cholerae putative NTPase EpsE==		==Crystal Structure of Vibrio cholerae putative NTPase EpsE==
-	<StructureSection load='1p9w' size='340' side='right' caption='[[1p9w]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='1p9w' size='340' side='right'caption='[[1p9w]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1p9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1P9W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1p9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1P9W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p9r\|1p9r]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1p9r\|1p9r]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [http://pdbe.org/1p9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [http://www.ebi.ac.uk/pdbsum/1p9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [http://pdbe.org/1p9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [http://www.ebi.ac.uk/pdbsum/1p9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 31:		Line 31:
	</div>		</div>
	<div class="pdbe-citations 1p9w" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1p9w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[General secretion pathway protein 3D structures\|General secretion pathway protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Bacillo virgola del koch trevisan 1884]]		[[Category: Bacillo virgola del koch trevisan 1884]]
		+	[[Category: Large Structures]]
	[[Category: Hol, W G.J]]		[[Category: Hol, W G.J]]
	[[Category: Krumm, B E]]		[[Category: Krumm, B E]]

OCA at 07:51, 15 February 2018

OCA — Thu, 15 Feb 2018 07:51:58 GMT

←Older revision		Revision as of 07:51, 15 February 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Vibrio cholerae putative NTPase EpsE==		==Crystal Structure of Vibrio cholerae putative NTPase EpsE==
	<StructureSection load='1p9w' size='340' side='right' caption='[[1p9w]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1p9w' size='340' side='right' caption='[[1p9w]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p9r\|1p9r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p9r\|1p9r]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [http://pdbe.org/1p9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [http://www.ebi.ac.uk/pdbsum/1p9w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [http://pdbe.org/1p9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [http://www.ebi.ac.uk/pdbsum/1p9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p9w_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p9w_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:22, 9 February 2016

OCA — Tue, 09 Feb 2016 08:22:37 GMT

←Older revision		Revision as of 08:22, 9 February 2016
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p9w ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:24, 10 September 2015

OCA — Thu, 10 Sep 2015 20:24:47 GMT

←Older revision		Revision as of 20:24, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1p9w' size='340' side='right' caption='[[1p9w]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1p9w' size='340' side='right' caption='[[1p9w]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1p9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_cholerae Vibrio cholerae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1P9W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1p9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1P9W FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p9r\|1p9r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p9r\|1p9r]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 ~~Vibrio cholerae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [http://www.ebi.ac.uk/pdbsum/1p9w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [http://pdbe.org/1p9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [http://www.ebi.ac.uk/pdbsum/1p9w PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1p9w" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio cholerae~~]]	+	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Hol, W G.J]]		[[Category: Hol, W G.J]]
	[[Category: Krumm, B E]]		[[Category: Krumm, B E]]

OCA at 05:40, 25 December 2014

OCA — Thu, 25 Dec 2014 05:40:58 GMT

←Older revision		Revision as of 05:40, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1p9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1P9W FirstGlance]. <br>		<table><tr><td colspan='2'>[[1p9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1P9W FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p9r\|1p9r]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p9r\|1p9r]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [http://www.ebi.ac.uk/pdbsum/1p9w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [http://www.ebi.ac.uk/pdbsum/1p9w PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GSPE_VIBCH GSPE_VIBCH]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
-	[[Category: Hol, W G.J.]]	+	[[Category: Hol, W G.J]]
-	[[Category: Krumm, B E.]]	+	[[Category: Krumm, B E]]
-	[[Category: Robien, M A.]]	+	[[Category: Robien, M A]]
-	[[Category: Sandkvist, M.]]	+	[[Category: Sandkvist, M]]
	[[Category: Atp binding protein]]		[[Category: Atp binding protein]]
	[[Category: Bacterial type ii secretion system cytoplasmic protein - gspe]]		[[Category: Bacterial type ii secretion system cytoplasmic protein - gspe]]
	[[Category: Protein transport]]		[[Category: Protein transport]]
	[[Category: Putative atpase]]		[[Category: Putative atpase]]

OCA at 23:00, 28 September 2014

OCA — Sun, 28 Sep 2014 23:00:06 GMT

←Older revision		Revision as of 23:00, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1p9w.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Vibrio cholerae putative NTPase EpsE==
		+	<StructureSection load='1p9w' size='340' side='right' caption='[[1p9w]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1p9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1P9W FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p9r\|1p9r]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EPSE OR VC2732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1p9w RCSB], [http://www.ebi.ac.uk/pdbsum/1p9w PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p9w_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type II secretion systems consist of an assembly of 12-15 Gsp proteins responsible for transporting a variety of virulence factors across the outer membrane in several pathogenic bacteria. In Vibrio cholerae, the major virulence factor cholera toxin is secreted by the Eps Type II secretion apparatus consisting of 14 Eps proteins. One of these, EpsE, is a cytoplasmic putative NTPase essential for the functioning of the Eps system and member of the GspE subfamily of Type II secretion ATPases. The crystal structure of a truncated form of EpsE in nucleotide-liganded and unliganded state has been determined, and reveals a two-domain architecture with the four characteristic sequence "boxes" of the GspE subfamily clustering around the nucleotide-binding site of the C-domain. This domain contains two C-terminal subdomains not reported before in this superfamily of NTPases. One of these subdomains contains a four-cysteine motif that appears to be involved in metal binding as revealed by anomalous difference density. The EpsE subunits form a right-handed helical arrangement in the crystal with extensive and conserved contacts between the C and N domains of neighboring subunits. Combining the most conserved interface with the quaternary structure of the C domain in a distant homolog, a hexameric model for EpsE is proposed which may reflect the assembly of this critical protein in the Type II secretion system. The nucleotide ligand contacts both domains in this model. The N2-domain-containing surface of the hexamer appears to be highly conserved in the GspE family and most likely faces the inner membrane interacting with other members of the Eps system.

-	~~{{STRUCTURE_1p9w\| PDB=1p9w \| SCENE= }}~~	+	Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae.,Robien MA, Krumm BE, Sandkvist M, Hol WG J Mol Biol. 2003 Oct 24;333(3):657-74. PMID:14556751<ref>PMID:14556751</ref>

-	~~===Crystal Structure~~ of ~~Vibrio cholerae putative NTPase EpsE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14556751}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1p9w]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Vibrio_cholerae Vibrio cholerae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014556751<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Hol, W G.J.]]		[[Category: Hol, W G.J.]]

OCA: Protected "1p9w" [edit=sysop:move=sysop]

OCA — Wed, 19 Dec 2012 11:58:29 GMT

Protected "1p9w" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:58, 19 December 2012

OCA at 11:58, 19 December 2012

OCA — Wed, 19 Dec 2012 11:58:26 GMT

←Older revision		Revision as of 11:58, 19 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1p9w.png\|left\|200px]]		[[Image:1p9w.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1p9w", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1p9w\| PDB=1p9w \| SCENE= }}		{{STRUCTURE_1p9w\| PDB=1p9w \| SCENE= }}

	===Crystal Structure of Vibrio cholerae putative NTPase EpsE===		===Crystal Structure of Vibrio cholerae putative NTPase EpsE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_14556751}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 14556751 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_14556751}}		{{ABSTRACT_PUBMED_14556751}}

	==About this Structure==		==About this Structure==
-	~~1P9W~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA].	+	[[1p9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14556751~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014556751</ref><references group="xtra"/>
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Hol, W G.J.]]		[[Category: Hol, W G.J.]]
Line 31:		Line 19:
	[[Category: Atp binding protein]]		[[Category: Atp binding protein]]
	[[Category: Bacterial type ii secretion system cytoplasmic protein - gspe]]		[[Category: Bacterial type ii secretion system cytoplasmic protein - gspe]]
		+	[[Category: Protein transport]]
	[[Category: Putative atpase]]		[[Category: Putative atpase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 08:19:30 2009''

OCA at 06:19, 17 February 2009

OCA — Tue, 17 Feb 2009 06:19:31 GMT

←Older revision		Revision as of 06:19, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1P9W is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA].	+	1P9W is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9W OCA].

	==Reference==		==Reference==
-	~~Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae., Robien MA, Krumm BE, Sandkvist M, Hol WG, J Mol Biol. 2003 Oct 24;333(3):657-74.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14556751 14556751]~~	+	<ref group="xtra">PMID:14556751</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Hol, W G.J.]]		[[Category: Hol, W G.J.]]
Line 34:		Line 33:
	[[Category: Putative atpase]]		[[Category: Putative atpase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 11~~:44:~~15 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 08:19:30 2009''