1pj8 - Revision history

OCA at 09:43, 16 August 2023

OCA — Wed, 16 Aug 2023 09:43:59 GMT

←Older revision		Revision as of 09:43, 16 August 2023
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	<StructureSection load='1pj8' size='340' side='right'caption='[[1pj8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1pj8' size='340' side='right'caption='[[1pj8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1pj8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Engyodontium_album Engyodontium~~ album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJ8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1pj8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJ8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~NH2~~:~~AMINO~~+~~GROUP~~'>~~NH2~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [https://pdbe.org/1pj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [https://www.ebi.ac.uk/pdbsum/1pj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pj8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [https://pdbe.org/1pj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [https://www.ebi.ac.uk/pdbsum/1pj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pj8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~PRTK_TRIAL PRTK_TRIAL]~~] Hydrolyzes keratin at aromatic and hydrophobic residues.	+	[https://www.uniprot.org/uniprot/PRTK_PARAQ PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 31:

	==See Also==		==See Also==
-	*[[Proteinase\|Proteinase]]
	*[[Proteinase 3D structures\|Proteinase 3D structures]]		*[[Proteinase 3D structures\|Proteinase 3D structures]]
	== References ==		== References ==
Line 38:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Engyodontium album]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Peptidase K~~]]	+	[[Category: Parengyodontium album]]
-	[[Category: Betzel, C]]	+	[[Category: Betzel C]]
-	[[Category: Fittkau, S]]	+	[[Category: Fittkau S]]
-	[[Category: Peters, K]]	+	[[Category: Peters K]]
-	[[Category: Saxena~~, A K~~]]	+	[[Category: Saxena AK]]
-	[[Category: Singh~~, T P~~]]	+	[[Category: Singh TP]]
-	[[Category: Visanji, M]]	+	[[Category: Visanji M]]
-	[[Category: Wilson~~, K S]]~~	+	[[Category: Wilson KS]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-hydrolase substrate complex]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Mercury]]~~	+
-	~~[[Category: Proteinase k]]~~	+
-	~~[[Category: Ternary complex~~]]	+

OCA at 13:16, 13 October 2021

OCA — Wed, 13 Oct 2021 13:16:49 GMT

←Older revision		Revision as of 13:16, 13 October 2021
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	<StructureSection load='1pj8' size='340' side='right'caption='[[1pj8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1pj8' size='340' side='right'caption='[[1pj8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1pj8]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1PJ8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1pj8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJ8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [~~http~~://pdbe.org/1pj8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1pj8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1pj8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [https://pdbe.org/1pj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [https://www.ebi.ac.uk/pdbsum/1pj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pj8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PRTK_TRIAL PRTK_TRIAL]] Hydrolyzes keratin at aromatic and hydrophobic residues.	+	[[https://www.uniprot.org/uniprot/PRTK_TRIAL PRTK_TRIAL]] Hydrolyzes keratin at aromatic and hydrophobic residues.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:20, 8 January 2020

OCA — Wed, 08 Jan 2020 10:20:07 GMT

←Older revision		Revision as of 10:20, 8 January 2020
Line 1:		Line 1:

	==Structure of a ternary complex of proteinase K, mercury and a substrate-analogue hexapeptide at 2.2 A resolution==		==Structure of a ternary complex of proteinase K, mercury and a substrate-analogue hexapeptide at 2.2 A resolution==
-	<StructureSection load='1pj8' size='340' side='right' caption='[[1pj8]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1pj8' size='340' side='right'caption='[[1pj8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pj8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PJ8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pj8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PJ8 FirstGlance]. <br>
Line 33:		Line 33:
	==See Also==		==See Also==
	*[[Proteinase\|Proteinase]]		*[[Proteinase\|Proteinase]]
		+	*[[Proteinase 3D structures\|Proteinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Engyodontium album]]		[[Category: Engyodontium album]]
		+	[[Category: Large Structures]]
	[[Category: Peptidase K]]		[[Category: Peptidase K]]
	[[Category: Betzel, C]]		[[Category: Betzel, C]]

OCA at 07:37, 15 February 2018

OCA — Thu, 15 Feb 2018 07:37:55 GMT

←Older revision		Revision as of 07:37, 15 February 2018
Line 1:		Line 1:
		+
	==Structure of a ternary complex of proteinase K, mercury and a substrate-analogue hexapeptide at 2.2 A resolution==		==Structure of a ternary complex of proteinase K, mercury and a substrate-analogue hexapeptide at 2.2 A resolution==
	<StructureSection load='1pj8' size='340' side='right' caption='[[1pj8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1pj8' size='340' side='right' caption='[[1pj8]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [http://pdbe.org/1pj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [http://www.ebi.ac.uk/pdbsum/1pj8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [http://pdbe.org/1pj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [http://www.ebi.ac.uk/pdbsum/1pj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1pj8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pj/1pj8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pj/1pj8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 16:36, 8 February 2016

OCA — Mon, 08 Feb 2016 16:36:31 GMT

←Older revision		Revision as of 16:36, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pj8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:27, 10 September 2015

OCA — Thu, 10 Sep 2015 13:27:54 GMT

←Older revision		Revision as of 13:27, 10 September 2015
Line 6:		Line 6:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [http://www.ebi.ac.uk/pdbsum/1pj8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [http://pdbe.org/1pj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [http://www.ebi.ac.uk/pdbsum/1pj8 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1pj8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 00:42, 25 December 2014

OCA — Thu, 25 Dec 2014 00:42:42 GMT

←Older revision		Revision as of 00:42, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pj8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PJ8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pj8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PJ8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [http://www.ebi.ac.uk/pdbsum/1pj8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [http://www.ebi.ac.uk/pdbsum/1pj8 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRTK_TRIAL PRTK_TRIAL]] Hydrolyzes keratin at aromatic and hydrophobic residues.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Engyodontium album]]		[[Category: Engyodontium album]]
	[[Category: Peptidase K]]		[[Category: Peptidase K]]
-	[[Category: Betzel, C.]]	+	[[Category: Betzel, C]]
-	[[Category: Fittkau, S.]]	+	[[Category: Fittkau, S]]
-	[[Category: Peters, K.]]	+	[[Category: Peters, K]]
-	[[Category: Saxena, A K.]]	+	[[Category: Saxena, A K]]
-	[[Category: Singh, T P.]]	+	[[Category: Singh, T P]]
-	[[Category: Visanji, M.]]	+	[[Category: Visanji, M]]
-	[[Category: Wilson, K S.]]	+	[[Category: Wilson, K S]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Hydrolase-hydrolase substrate complex]]		[[Category: Hydrolase-hydrolase substrate complex]]

OCA at 22:21, 28 September 2014

OCA — Sun, 28 Sep 2014 22:21:14 GMT

←Older revision		Revision as of 22:21, 28 September 2014
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-	[[~~Image:~~1pj8.~~png~~\|~~left~~\|200px]]	+	==Structure of a ternary complex of proteinase K, mercury and a substrate-analogue hexapeptide at 2.2 A resolution==
		+	<StructureSection load='1pj8' size='340' side='right' caption='[[1pj8]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1pj8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PJ8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pj8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pj8 RCSB], [http://www.ebi.ac.uk/pdbsum/1pj8 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pj/1pj8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of a ternary complex of proteinase K, Hg(II) and a hexapeptide N-Ac-Pro-Ala-Pro-Phe-Pro-Ala-NH2 has been determined at 2.2 A resolution and refined to an R factor of 0.172 for 12,910 reflections. The mercury atom occupies two alternate sites, each of which was assigned an occupancy of 0.45. These two sites are bridged by Cys-73 S gamma which forms covalent bonds to both. Both mercury sites form regular polyhedrons involving atoms from residues Asp-39, His-69, Cys-73, His-72, Met-225, and Wat-324. The complex formation with mercury seems to disturb the stereochemistry of the residues of the catalytic triad Asp-39, His-69, and Ser-224 appreciably, thus reducing the enzymatic activity of proteinase K to 15%. The electron density in the difference Fourier map shows that the hexapeptide occupies the S1 subsite predominantly and the standard recognition site constituted by Ser-132 to Gly-136 and Gly-100 to Tyr-104 segments is virtually empty. The hexapeptide is held firmly through a series of hydrogen bonds involving protein atoms and water molecules. As a result of complex formation, Asp-39, His-69, Met-225, Ile-220, Ser-219, Thr-223, and Ser-224 residues move appreciably to accommodate the mercury atoms and the hexapeptide. The largest movement is observed for Met-225 which is involved in multiple interactions with both mercury and the hexapeptide. The activity results indicate an inhibition rate of 95%, as a result of the combined effect of mercury and hexapeptide.

-	~~{{STRUCTURE_1pj8\| PDB=1pj8 \| SCENE= }}~~	+	Structure of a ternary complex of proteinase K, mercury, and a substrate-analogue hexa-peptide at 2.2 A resolution.,Saxena AK, Singh TP, Peters K, Fittkau S, Visanji M, Wilson KS, Betzel C Proteins. 1996 Jun;25(2):195-201. PMID:8811735<ref>PMID:8811735</ref>

-	~~===Structure of a ternary complex of proteinase K~~, ~~mercury and~~ a ~~substrate-analogue hexapeptide at 2~~.~~2 A resolution===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_8811735}}~~	+	==See Also==
-		+	*[[Proteinase\|Proteinase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1pj8~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:008811735<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Engyodontium album]]		[[Category: Engyodontium album]]
	[[Category: Peptidase K]]		[[Category: Peptidase K]]

OCA: Protected "1pj8" [edit=sysop:move=sysop]

OCA — Wed, 19 Dec 2012 12:06:53 GMT

Protected "1pj8" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:06, 19 December 2012

OCA at 12:06, 19 December 2012

OCA — Wed, 19 Dec 2012 12:06:49 GMT

←Older revision		Revision as of 12:06, 19 December 2012
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-	{{Seed}}
	[[Image:1pj8.png\|left\|200px]]		[[Image:1pj8.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
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	{{STRUCTURE_1pj8\| PDB=1pj8 \| SCENE= }}		{{STRUCTURE_1pj8\| PDB=1pj8 \| SCENE= }}

	===Structure of a ternary complex of proteinase K, mercury and a substrate-analogue hexapeptide at 2.2 A resolution===		===Structure of a ternary complex of proteinase K, mercury and a substrate-analogue hexapeptide at 2.2 A resolution===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_8811735}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 8811735 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_8811735}}		{{ABSTRACT_PUBMED_8811735}}

	==About this Structure==		==About this Structure==
-	~~1PJ8~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA].	+	[[1pj8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJ8 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~8811735~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008811735</ref><references group="xtra"/>
	[[Category: Engyodontium album]]		[[Category: Engyodontium album]]
	[[Category: Peptidase K]]		[[Category: Peptidase K]]
Line 33:		Line 21:
	[[Category: Visanji, M.]]		[[Category: Visanji, M.]]
	[[Category: Wilson, K S.]]		[[Category: Wilson, K S.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-hydrolase substrate complex]]
	[[Category: Inhibitor]]		[[Category: Inhibitor]]
	[[Category: Mercury]]		[[Category: Mercury]]
	[[Category: Proteinase k]]		[[Category: Proteinase k]]
-	[[Category: Structure]]
	[[Category: Ternary complex]]		[[Category: Ternary complex]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:29:44 2009''