1pnf - Revision history

OCA at 08:46, 11 October 2023

OCA — Wed, 11 Oct 2023 08:46:39 GMT

←Older revision		Revision as of 08:46, 11 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PNF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PNF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~"><~~scene name~~='~~pdbligand~~=~~SO4~~:~~SULFATE+ION'~~>~~SO4~~</~~scene~~>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene><~~/td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>[https://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [https://pdbe.org/1pnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [https://www.ebi.ac.uk/pdbsum/1pnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pnf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [https://pdbe.org/1pnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [https://www.ebi.ac.uk/pdbsum/1pnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pnf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PNGF_ELIMR PNGF_ELIMR]] Cleaves an entire glycan from a glycoprotein. Requires that the glycosylated asparagine moiety (reaction 1) be substituted on its amino (R1) and carboxyl (R2) terminus with a polypeptide chain.	+	[https://www.uniprot.org/uniprot/PNGF_ELIMR PNGF_ELIMR] Cleaves an entire glycan from a glycoprotein. Requires that the glycosylated asparagine moiety (reaction 1) be substituted on its amino (R1) and carboxyl (R2) terminus with a polypeptide chain.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 28:
	[[Category: Elizabethkingia meningoseptica]]		[[Category: Elizabethkingia meningoseptica]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kuhn, P]]	+	[[Category: Kuhn P]]
-	[[Category: Roey, P ~~Van]]~~	+	[[Category: Van Roey P]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 10:58, 5 January 2022

OCA — Wed, 05 Jan 2022 10:58:18 GMT

←Older revision		Revision as of 10:58, 5 January 2022
Line 3:		Line 3:
	<StructureSection load='1pnf' size='340' side='right'caption='[[1pnf]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1pnf' size='340' side='right'caption='[[1pnf]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1PNF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PNF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG~~</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [~~http~~://pdbe.org/1pnf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1pnf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1pnf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [https://pdbe.org/1pnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [https://www.ebi.ac.uk/pdbsum/1pnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pnf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PNGF_ELIMR PNGF_ELIMR]] Cleaves an entire glycan from a glycoprotein. Requires that the glycosylated asparagine moiety (reaction 1) be substituted on its amino (R1) and carboxyl (R2) terminus with a polypeptide chain.	+	[[https://www.uniprot.org/uniprot/PNGF_ELIMR PNGF_ELIMR]] Cleaves an entire glycan from a glycoprotein. Requires that the glycosylated asparagine moiety (reaction 1) be substituted on its amino (R1) and carboxyl (R2) terminus with a polypeptide chain.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:58, 1 April 2020

OCA — Wed, 01 Apr 2020 09:58:43 GMT

←Older revision		Revision as of 09:58, 1 April 2020
Line 1:		Line 1:

	==PNGASE F COMPLEX WITH DI-N-ACETYLCHITOBIOSE==		==PNGASE F COMPLEX WITH DI-N-ACETYLCHITOBIOSE==
-	<StructureSection load='1pnf' size='340' side='right' caption='[[1pnf]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1pnf' size='340' side='right'caption='[[1pnf]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PNF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PNF FirstGlance]. <br>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Elizabethkingia meningoseptica]]		[[Category: Elizabethkingia meningoseptica]]
		+	[[Category: Large Structures]]
	[[Category: Kuhn, P]]		[[Category: Kuhn, P]]
	[[Category: Roey, P Van]]		[[Category: Roey, P Van]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 05:43, 27 June 2018

OCA — Wed, 27 Jun 2018 05:43:22 GMT

←Older revision		Revision as of 05:43, 27 June 2018
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 1pnf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1pnf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Peptide N-glycanase\|Peptide N-glycanase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:52, 29 November 2017

OCA — Wed, 29 Nov 2017 06:52:11 GMT

←Older revision		Revision as of 06:52, 29 November 2017
Line 1:		Line 1:
		+
	==PNGASE F COMPLEX WITH DI-N-ACETYLCHITOBIOSE==		==PNGASE F COMPLEX WITH DI-N-ACETYLCHITOBIOSE==
	<StructureSection load='1pnf' size='340' side='right' caption='[[1pnf]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1pnf' size='340' side='right' caption='[[1pnf]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PNF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PNF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>,~~ <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [http://pdbe.org/1pnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [http://www.ebi.ac.uk/pdbsum/1pnf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [http://pdbe.org/1pnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [http://www.ebi.ac.uk/pdbsum/1pnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1pnf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 1pnf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1pnf" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Peptide N-glycanase\|Peptide N-glycanase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 03:22, 11 September 2015

OCA — Fri, 11 Sep 2015 03:22:15 GMT

←Older revision		Revision as of 03:22, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [http://www.ebi.ac.uk/pdbsum/1pnf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [http://pdbe.org/1pnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [http://www.ebi.ac.uk/pdbsum/1pnf PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1pnf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Peptide N-glycanase\|Peptide N-glycanase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:25, 25 December 2014

OCA — Thu, 25 Dec 2014 10:25:23 GMT

←Older revision		Revision as of 10:25, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PNF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PNF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [http://www.ebi.ac.uk/pdbsum/1pnf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [http://www.ebi.ac.uk/pdbsum/1pnf PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PNGF_ELIMR PNGF_ELIMR]] Cleaves an entire glycan from a glycoprotein. Requires that the glycosylated asparagine moiety (reaction 1) be substituted on its amino (R1) and carboxyl (R2) terminus with a polypeptide chain.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Elizabethkingia meningoseptica]]		[[Category: Elizabethkingia meningoseptica]]
-	[[Category: Kuhn, P.]]	+	[[Category: Kuhn, P]]
-	[[Category: Roey, P Van.]]	+	[[Category: Roey, P Van]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 23:26, 28 September 2014

OCA — Sun, 28 Sep 2014 23:26:46 GMT

←Older revision		Revision as of 23:26, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1pnf.~~png~~\|~~left\|200px~~]]	+	==PNGASE F COMPLEX WITH DI-N-ACETYLCHITOBIOSE==
		+	<StructureSection load='1pnf' size='340' side='right' caption='[[1pnf]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1pnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PNF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pnf RCSB], [http://www.ebi.ac.uk/pdbsum/1pnf PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Crystallographic analysis and site-directed mutagenesis have been used to identify the catalytic and oligosaccharide recognition residues of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F (PNGase F), an amidohydrolase that removes intact asparagine-linked oligosaccharide chains from glycoproteins and glycopeptides. Mutagenesis has shown that three acidic residues, Asp-60, Glu-206, and Glu-118, that are located in a cleft at the interface between the two domains of the protein are essential for activity. The D60N mutant has no detectable activity, while E206Q and E118Q have less than 0.01 and 0.1% of the wild-type activity, respectively. Crystallographic analysis, at 2.0-A resolution, of the complex of the wild-type enzyme with the product, N,N'-diacetylchitobiose, shows that Asp-60 is in direct contact with the substrate at the cleavage site, while Glu-206 makes contact through a bridging water molecule. This indicates that Asp-60 is the primary catalytic residue, while Glu-206 probably is important for stabilization of reaction intermediates. Glu-118 forms a hydrogen bond with O6 of the second N-acetylglucosamine residue of the substrate and the low activity of the E118Q mutant results from its reduced ability to bind the oligosaccharide. This analysis also suggests that the mechanism of action of PNGase F differs from those of L-asparaginase and glycosylasparaginase, which involve a threonine residue as the nucleophile.

-	~~{{STRUCTURE_1pnf\| PDB=1pnf \| SCENE= }}~~	+	Active site and oligosaccharide recognition residues of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F.,Kuhn P, Guan C, Cui T, Tarentino AL, Plummer TH Jr, Van Roey P J Biol Chem. 1995 Dec 8;270(49):29493-7. PMID:7493989<ref>PMID:7493989</ref>

-	~~===PNGASE F COMPLEX WITH DI-N-ACETYLCHITOBIOSE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7493989}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1pnf]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA].~~	+	</StructureSection>
	[[Category: Elizabethkingia meningoseptica]]		[[Category: Elizabethkingia meningoseptica]]
	[[Category: Kuhn, P.]]		[[Category: Kuhn, P.]]
	[[Category: Roey, P Van.]]		[[Category: Roey, P Van.]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA: Protected "1pnf" [edit=sysop:move=sysop]

OCA — Wed, 19 Dec 2012 11:49:13 GMT

Protected "1pnf" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:49, 19 December 2012

OCA at 11:49, 19 December 2012

OCA — Wed, 19 Dec 2012 11:49:11 GMT

←Older revision		Revision as of 11:49, 19 December 2012
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-	{{Seed}}
	[[Image:1pnf.png\|left\|200px]]		[[Image:1pnf.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1pnf\| PDB=1pnf \| SCENE= }}		{{STRUCTURE_1pnf\| PDB=1pnf \| SCENE= }}

	===PNGASE F COMPLEX WITH DI-N-ACETYLCHITOBIOSE===		===PNGASE F COMPLEX WITH DI-N-ACETYLCHITOBIOSE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_7493989}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 7493989 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_7493989}}		{{ABSTRACT_PUBMED_7493989}}

	==About this Structure==		==About this Structure==
-	~~1PNF is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA].	+	[[1pnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNF OCA].
-		+
-	~~==Reference==~~	+
-	Active site and oligosaccharide recognition residues of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F., Kuhn P, Guan C, Cui T, Tarentino AL, Plummer TH Jr, Van Roey P, J Biol Chem. 1995 Dec 8;270(49):29493-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7493989 7493989]	+
	[[Category: Elizabethkingia meningoseptica]]		[[Category: Elizabethkingia meningoseptica]]
-	[[Category: Single protein]]
	[[Category: Kuhn, P.]]		[[Category: Kuhn, P.]]
	[[Category: Roey, P Van.]]		[[Category: Roey, P Van.]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:04:12 2008''