1prt - Revision history

OCA at 05:58, 17 April 2024

OCA — Wed, 17 Apr 2024 05:58:28 GMT

←Older revision		Revision as of 05:58, 17 April 2024
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	<StructureSection load='1prt' size='340' side='right'caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1prt' size='340' side='right'caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927~~]. The September 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PRT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis]. The September 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PRT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [https://pdbe.org/1prt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [https://www.ebi.ac.uk/pdbsum/1prt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1prt ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [https://pdbe.org/1prt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [https://www.ebi.ac.uk/pdbsum/1prt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1prt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TOX4_BORPE TOX4_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[https://www.uniprot.org/uniprot/TOX1_BORPE TOX1_BORPE]] S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors. [[https://www.uniprot.org/uniprot/TOX3_BORPE TOX3_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[https://www.uniprot.org/uniprot/TOX2_BORPE TOX2_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[https://www.uniprot.org/uniprot/TOX5_BORPE TOX5_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane.	+	[https://www.uniprot.org/uniprot/TOX1_BORPE TOX1_BORPE] S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1prt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1prt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: Pertussis toxin is an exotoxin of the A-B class produced by Bordetella pertussis. The holotoxin comprises 952 residues forming six subunits (five different sequences, S1-S5). It plays an important role in the development of protective immunity to whooping cough, and is an essential component of new acellular vaccines. It is also widely used as a biochemical tool to ADP-ribosylate GTP-binding proteins in the study of signal transduction. RESULTS: The crystal structure of pertussis toxin has been determined at 2.9 A resolution. The catalytic A-subunit (S1) shares structural homology with other ADP-ribosylating bacterial toxins, although differences in the carboxy-terminal portion explain its unique activation mechanism. Despite its heterogeneous subunit composition, the structure of the cell-binding B-oligomer (S2, S3, two copies of S4, and S5) resembles the symmetrical B-pentamers of the cholera toxin and Shiga toxin families, but it interacts differently with the A-subunit. The structural similarity is all the more surprising given that there is almost no sequence homology between B-subunits of the different toxins. Two peripheral domains that are unique to the pertussis toxin B-oligomer show unexpected structural homology with a calcium-dependent eukaryotic lectin, and reveal possible receptor-binding sites. CONCLUSION: The structure provides insight into the pathogenic mechanisms of pertussis toxin and the evolution of bacterial toxins. Knowledge of the tertiary structure of the active site forms a rational basis for elimination of catalytic activity in recombinant molecules for vaccine use.
-
-	The crystal structure of pertussis toxin.,Stein PE, Boodhoo A, Armstrong GD, Cockle SA, Klein MH, Read RJ Structure. 1994 Jan 15;2(1):45-57. PMID:8075982<ref>PMID:8075982</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1prt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Pertussis toxin\|Pertussis toxin]]		*[[Pertussis toxin\|Pertussis toxin]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacterium tussis-convulsivae lehmann and neumann 1927~~]]	+	[[Category: Bordetella pertussis]]
	[[Category: Cholera Toxin]]		[[Category: Cholera Toxin]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Read~~, R J~~]]	+	[[Category: Read RJ]]
-	[[Category: Stein~~, P E]]~~	+	[[Category: Stein PE]]
-	~~[[Category: Toxin~~]]	+

OCA at 09:33, 8 September 2021

OCA — Wed, 08 Sep 2021 09:33:53 GMT

←Older revision		Revision as of 09:33, 8 September 2021
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	<StructureSection load='1prt' size='340' side='right'caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1prt' size='340' side='right'caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927]. The September 2005 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1PRT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927]. The September 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PRT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [~~http~~://pdbe.org/1prt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1prt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1prt ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [https://pdbe.org/1prt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [https://www.ebi.ac.uk/pdbsum/1prt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1prt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TOX4_BORPE TOX4_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[~~http~~://www.uniprot.org/uniprot/TOX1_BORPE TOX1_BORPE]] S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors. [[~~http~~://www.uniprot.org/uniprot/TOX3_BORPE TOX3_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[~~http~~://www.uniprot.org/uniprot/TOX2_BORPE TOX2_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[~~http~~://www.uniprot.org/uniprot/TOX5_BORPE TOX5_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane.	+	[[https://www.uniprot.org/uniprot/TOX4_BORPE TOX4_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[https://www.uniprot.org/uniprot/TOX1_BORPE TOX1_BORPE]] S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors. [[https://www.uniprot.org/uniprot/TOX3_BORPE TOX3_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[https://www.uniprot.org/uniprot/TOX2_BORPE TOX2_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[https://www.uniprot.org/uniprot/TOX5_BORPE TOX5_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:59, 27 November 2019

OCA — Wed, 27 Nov 2019 09:59:46 GMT

←Older revision		Revision as of 09:59, 27 November 2019
Line 1:		Line 1:

	==THE CRYSTAL STRUCTURE OF PERTUSSIS TOXIN==		==THE CRYSTAL STRUCTURE OF PERTUSSIS TOXIN==
-	<StructureSection load='1prt' size='340' side='right' caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='1prt' size='340' side='right'caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PRT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PRT FirstGlance]. <br>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1prt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1prt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Pertussis toxin\|Pertussis toxin]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	[[Category: Bacterium tussis-convulsivae lehmann and neumann 1927]]		[[Category: Bacterium tussis-convulsivae lehmann and neumann 1927]]
	[[Category: Cholera Toxin]]		[[Category: Cholera Toxin]]
		+	[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Read, R J]]		[[Category: Read, R J]]
	[[Category: Stein, P E]]		[[Category: Stein, P E]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 08:01, 24 February 2018

OCA — Sat, 24 Feb 2018 08:01:47 GMT

←Older revision		Revision as of 08:01, 24 February 2018
Line 1:		Line 1:
		+
	==THE CRYSTAL STRUCTURE OF PERTUSSIS TOXIN==		==THE CRYSTAL STRUCTURE OF PERTUSSIS TOXIN==
	<StructureSection load='1prt' size='340' side='right' caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1prt' size='340' side='right' caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PRT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PRT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [http://pdbe.org/1prt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [http://www.ebi.ac.uk/pdbsum/1prt PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [http://pdbe.org/1prt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [http://www.ebi.ac.uk/pdbsum/1prt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1prt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/1prt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/1prt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1prt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1prt" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Pertussis toxin\|Pertussis toxin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:33, 8 February 2016

OCA — Mon, 08 Feb 2016 21:33:37 GMT

←Older revision		Revision as of 21:33, 8 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1prt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:35, 10 September 2015

OCA — Thu, 10 Sep 2015 15:35:15 GMT

←Older revision		Revision as of 15:35, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1prt' size='340' side='right' caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1prt' size='340' side='right' caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bordetella_pertussis Bordetella pertussis~~]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PRT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_tussis-convulsivae"_lehmann_and_neumann_1927 "bacterium tussis-convulsivae" lehmann and neumann 1927]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PRT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [http://www.ebi.ac.uk/pdbsum/1prt PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [http://pdbe.org/1prt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [http://www.ebi.ac.uk/pdbsum/1prt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1prt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bordetella pertussis~~]]	+	[[Category: Bacterium tussis-convulsivae lehmann and neumann 1927]]
	[[Category: Cholera Toxin]]		[[Category: Cholera Toxin]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]

OCA at 02:17, 25 December 2014

OCA — Thu, 25 Dec 2014 02:17:33 GMT

←Older revision		Revision as of 02:17, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PRT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PRT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [http://www.ebi.ac.uk/pdbsum/1prt PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [http://www.ebi.ac.uk/pdbsum/1prt PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TOX4_BORPE TOX4_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[http://www.uniprot.org/uniprot/TOX1_BORPE TOX1_BORPE]] S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors. [[http://www.uniprot.org/uniprot/TOX3_BORPE TOX3_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[http://www.uniprot.org/uniprot/TOX2_BORPE TOX2_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane. [[http://www.uniprot.org/uniprot/TOX5_BORPE TOX5_BORPE]] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	[[Category: Cholera Toxin]]		[[Category: Cholera Toxin]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Read, R J.]]	+	[[Category: Read, R J]]
-	[[Category: Stein, P E.]]	+	[[Category: Stein, P E]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 23:50, 28 September 2014

OCA — Sun, 28 Sep 2014 23:50:26 GMT

←Older revision		Revision as of 23:50, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1prt.~~png\|left~~\|200px]]	+	==THE CRYSTAL STRUCTURE OF PERTUSSIS TOXIN==
		+	<StructureSection load='1prt' size='340' side='right' caption='[[1prt]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1prt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PRT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1prt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1prt RCSB], [http://www.ebi.ac.uk/pdbsum/1prt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/1prt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Pertussis toxin is an exotoxin of the A-B class produced by Bordetella pertussis. The holotoxin comprises 952 residues forming six subunits (five different sequences, S1-S5). It plays an important role in the development of protective immunity to whooping cough, and is an essential component of new acellular vaccines. It is also widely used as a biochemical tool to ADP-ribosylate GTP-binding proteins in the study of signal transduction. RESULTS: The crystal structure of pertussis toxin has been determined at 2.9 A resolution. The catalytic A-subunit (S1) shares structural homology with other ADP-ribosylating bacterial toxins, although differences in the carboxy-terminal portion explain its unique activation mechanism. Despite its heterogeneous subunit composition, the structure of the cell-binding B-oligomer (S2, S3, two copies of S4, and S5) resembles the symmetrical B-pentamers of the cholera toxin and Shiga toxin families, but it interacts differently with the A-subunit. The structural similarity is all the more surprising given that there is almost no sequence homology between B-subunits of the different toxins. Two peripheral domains that are unique to the pertussis toxin B-oligomer show unexpected structural homology with a calcium-dependent eukaryotic lectin, and reveal possible receptor-binding sites. CONCLUSION: The structure provides insight into the pathogenic mechanisms of pertussis toxin and the evolution of bacterial toxins. Knowledge of the tertiary structure of the active site forms a rational basis for elimination of catalytic activity in recombinant molecules for vaccine use.

-	~~{{STRUCTURE_1prt\| PDB=1prt \| SCENE= }}~~	+	The crystal structure of pertussis toxin.,Stein PE, Boodhoo A, Armstrong GD, Cockle SA, Klein MH, Read RJ Structure. 1994 Jan 15;2(1):45-57. PMID:8075982<ref>PMID:8075982</ref>

-	~~===THE CRYSTAL STRUCTURE OF PERTUSSIS TOXIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8075982}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1prt]] is~~ a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the ~~Month] feature on ''Cholera Toxin'' by David~~ S. ~~Goodsell is [http://dx~~.~~doi.org~~/~~10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA].~~	+

	==See Also==		==See Also==
	*[[Pertussis toxin\|Pertussis toxin]]		*[[Pertussis toxin\|Pertussis toxin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:008075982<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bordetella pertussis]]		[[Category: Bordetella pertussis]]
	[[Category: Cholera Toxin]]		[[Category: Cholera Toxin]]

OCA at 11:32, 20 October 2012

OCA — Sat, 20 Oct 2012 11:32:49 GMT

←Older revision		Revision as of 11:32, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1prt]] is a 12 chain structure ~~of [[Pertussis toxin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA].	+	[[1prt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis]. The September 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cholera Toxin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_9 10.2210/rcsb_pdb/mom_2005_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRT OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:008075982~~</ref><ref group="xtra">PMID:011170211~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008075982</ref><references group="xtra"/>
	[[Category: Bordetella pertussis]]		[[Category: Bordetella pertussis]]
	[[Category: Cholera Toxin]]		[[Category: Cholera Toxin]]

OCA: Protected "1prt" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 05:44:42 GMT

Protected "1prt" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:44, 26 July 2012