1pta - Revision history

OCA at 08:10, 14 February 2024

OCA — Wed, 14 Feb 2024 08:10:41 GMT

←Older revision		Revision as of 08:10, 14 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PTA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PTA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [https://pdbe.org/1pta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [https://www.ebi.ac.uk/pdbsum/1pta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pta ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [https://pdbe.org/1pta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [https://www.ebi.ac.uk/pdbsum/1pta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pta ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/OPD_BREDI OPD_BREDI]] Has an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates. All of the phosphate triesters found to be substrates are synthetic compounds. The identity of any naturally occurring substrate for the enzyme is unknown. Has no detectable activity with phosphate monoesters or diesters and no activity as an esterase or protease. It catalyzes the hydrolysis of the insecticide paraoxon at a rate approaching the diffusion limit and thus appears to be optimally evolved for utilizing this synthetic substrate.	+	[https://www.uniprot.org/uniprot/OPD_BREDI OPD_BREDI] Has an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates. All of the phosphate triesters found to be substrates are synthetic compounds. The identity of any naturally occurring substrate for the enzyme is unknown. Has no detectable activity with phosphate monoesters or diesters and no activity as an esterase or protease. It catalyzes the hydrolysis of the insecticide paraoxon at a rate approaching the diffusion limit and thus appears to be optimally evolved for utilizing this synthetic substrate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pta ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pta ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Organophosphates, such as parathion and paraoxon, constitute the largest class of insecticides currently used in industrialized nations. In addition, many of these compounds are known to inhibit mammalian acetylcholinesterases thereby acting as nerve agents. Consequently, organophosphate-degrading enzymes are of considerable interest in light of their ability to detoxify such compounds. Here we report the three-dimensional structure of such an enzyme, namely, phosphotriesterase, as determined by single crystal X-ray diffraction analysis to 2.1-A resolution. Crystals employed in this investigation belonged to the space group P2(1)2(1)2 with unit cell dimensions of a = 80.3 A, b = 93.4 A, and c = 44.8 A and one molecule per asymmetric unit. The structure was solved by multiple isomorphous replacement with two heavy-atom derivatives and refined to a crystallographic R factor of 18.0%. As observed in various other enzymes, the overall fold of the molecule consists of an alpha/beta barrel with eight strands of parallel beta-pleated sheet. In addition, there are two antiparallel beta-strands at the N-terminus. The molecular model of phosphotriesterase presented here provides the initial structural framework necessary toward understanding the enzyme's broad substrate specificities and its catalytic mechanism.
-
-	Three-dimensional structure of phosphotriesterase: an enzyme capable of detoxifying organophosphate nerve agents.,Benning MM, Kuo JM, Raushel FM, Holden HM Biochemistry. 1994 Dec 20;33(50):15001-7. PMID:7999757<ref>PMID:7999757</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1pta" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Phosphotriesterase 3D structures\|Phosphotriesterase 3D structures]]		*[[Phosphotriesterase 3D structures\|Phosphotriesterase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Brevundimonas diminuta]]		[[Category: Brevundimonas diminuta]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Benning, M]]	+	[[Category: Benning M]]
-	[[Category: Holden~~, H M~~]]	+	[[Category: Holden HM]]

OCA at 09:34, 8 September 2021

OCA — Wed, 08 Sep 2021 09:34:34 GMT

←Older revision		Revision as of 09:34, 8 September 2021
Line 3:		Line 3:
	<StructureSection load='1pta' size='340' side='right'caption='[[1pta]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1pta' size='340' side='right'caption='[[1pta]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1PTA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PTA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [~~http~~://pdbe.org/1pta PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1pta PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1pta ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [https://pdbe.org/1pta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [https://www.ebi.ac.uk/pdbsum/1pta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pta ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/OPD_BREDI OPD_BREDI]] Has an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates. All of the phosphate triesters found to be substrates are synthetic compounds. The identity of any naturally occurring substrate for the enzyme is unknown. Has no detectable activity with phosphate monoesters or diesters and no activity as an esterase or protease. It catalyzes the hydrolysis of the insecticide paraoxon at a rate approaching the diffusion limit and thus appears to be optimally evolved for utilizing this synthetic substrate.	+	[[https://www.uniprot.org/uniprot/OPD_BREDI OPD_BREDI]] Has an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates. All of the phosphate triesters found to be substrates are synthetic compounds. The identity of any naturally occurring substrate for the enzyme is unknown. Has no detectable activity with phosphate monoesters or diesters and no activity as an esterase or protease. It catalyzes the hydrolysis of the insecticide paraoxon at a rate approaching the diffusion limit and thus appears to be optimally evolved for utilizing this synthetic substrate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:57, 27 November 2019

OCA — Wed, 27 Nov 2019 09:57:27 GMT

←Older revision		Revision as of 09:57, 27 November 2019
Line 1:		Line 1:

	==THREE-DIMENSIONAL STRUCTURE OF PHOSPHOTRIESTERASE: AN ENZYME CAPABLE OF DETOXIFYING ORGANOPHOSPHATE NERVE AGENTS==		==THREE-DIMENSIONAL STRUCTURE OF PHOSPHOTRIESTERASE: AN ENZYME CAPABLE OF DETOXIFYING ORGANOPHOSPHATE NERVE AGENTS==
-	<StructureSection load='1pta' size='340' side='right' caption='[[1pta]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1pta' size='340' side='right'caption='[[1pta]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PTA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PTA FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/1pta_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/1pta_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1pta" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1pta" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphotriesterase 3D structures\|Phosphotriesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Brevundimonas diminuta]]		[[Category: Brevundimonas diminuta]]
		+	[[Category: Large Structures]]
	[[Category: Benning, M]]		[[Category: Benning, M]]
	[[Category: Holden, H M]]		[[Category: Holden, H M]]

OCA at 06:52, 29 November 2017

OCA — Wed, 29 Nov 2017 06:52:47 GMT

←Older revision		Revision as of 06:52, 29 November 2017
Line 1:		Line 1:
		+
	==THREE-DIMENSIONAL STRUCTURE OF PHOSPHOTRIESTERASE: AN ENZYME CAPABLE OF DETOXIFYING ORGANOPHOSPHATE NERVE AGENTS==		==THREE-DIMENSIONAL STRUCTURE OF PHOSPHOTRIESTERASE: AN ENZYME CAPABLE OF DETOXIFYING ORGANOPHOSPHATE NERVE AGENTS==
	<StructureSection load='1pta' size='340' side='right' caption='[[1pta]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1pta' size='340' side='right' caption='[[1pta]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PTA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PTA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [http://pdbe.org/1pta PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [http://www.ebi.ac.uk/pdbsum/1pta PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [http://pdbe.org/1pta PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [http://www.ebi.ac.uk/pdbsum/1pta PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1pta ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1pta" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1pta" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Phosphotriesterase\|Phosphotriesterase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:29, 8 February 2016

OCA — Mon, 08 Feb 2016 14:29:33 GMT

←Older revision		Revision as of 14:29, 8 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pta ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:28, 10 September 2015

OCA — Thu, 10 Sep 2015 12:28:56 GMT

←Older revision		Revision as of 12:28, 10 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PTA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PTA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [http://www.ebi.ac.uk/pdbsum/1pta PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [http://pdbe.org/1pta PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [http://www.ebi.ac.uk/pdbsum/1pta PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1pta" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 00:01, 25 December 2014

OCA — Thu, 25 Dec 2014 00:01:03 GMT

←Older revision		Revision as of 00:01, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PTA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PTA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [http://www.ebi.ac.uk/pdbsum/1pta PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [http://www.ebi.ac.uk/pdbsum/1pta PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/OPD_BREDI OPD_BREDI]] Has an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates. All of the phosphate triesters found to be substrates are synthetic compounds. The identity of any naturally occurring substrate for the enzyme is unknown. Has no detectable activity with phosphate monoesters or diesters and no activity as an esterase or protease. It catalyzes the hydrolysis of the insecticide paraoxon at a rate approaching the diffusion limit and thus appears to be optimally evolved for utilizing this synthetic substrate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Brevundimonas diminuta]]		[[Category: Brevundimonas diminuta]]
-	[[Category: Benning, M.]]	+	[[Category: Benning, M]]
-	[[Category: Holden, H M.]]	+	[[Category: Holden, H M]]

OCA at 21:37, 28 September 2014

OCA — Sun, 28 Sep 2014 21:37:31 GMT

←Older revision		Revision as of 21:37, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1pta.~~png\|left~~\|200px]]	+	==THREE-DIMENSIONAL STRUCTURE OF PHOSPHOTRIESTERASE: AN ENZYME CAPABLE OF DETOXIFYING ORGANOPHOSPHATE NERVE AGENTS==
		+	<StructureSection load='1pta' size='340' side='right' caption='[[1pta]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1pta]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PTA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pta OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pta RCSB], [http://www.ebi.ac.uk/pdbsum/1pta PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/1pta_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Organophosphates, such as parathion and paraoxon, constitute the largest class of insecticides currently used in industrialized nations. In addition, many of these compounds are known to inhibit mammalian acetylcholinesterases thereby acting as nerve agents. Consequently, organophosphate-degrading enzymes are of considerable interest in light of their ability to detoxify such compounds. Here we report the three-dimensional structure of such an enzyme, namely, phosphotriesterase, as determined by single crystal X-ray diffraction analysis to 2.1-A resolution. Crystals employed in this investigation belonged to the space group P2(1)2(1)2 with unit cell dimensions of a = 80.3 A, b = 93.4 A, and c = 44.8 A and one molecule per asymmetric unit. The structure was solved by multiple isomorphous replacement with two heavy-atom derivatives and refined to a crystallographic R factor of 18.0%. As observed in various other enzymes, the overall fold of the molecule consists of an alpha/beta barrel with eight strands of parallel beta-pleated sheet. In addition, there are two antiparallel beta-strands at the N-terminus. The molecular model of phosphotriesterase presented here provides the initial structural framework necessary toward understanding the enzyme's broad substrate specificities and its catalytic mechanism.

-	~~{{STRUCTURE_1pta\| PDB=1pta \| SCENE= }}~~	+	Three-dimensional structure of phosphotriesterase: an enzyme capable of detoxifying organophosphate nerve agents.,Benning MM, Kuo JM, Raushel FM, Holden HM Biochemistry. 1994 Dec 20;33(50):15001-7. PMID:7999757<ref>PMID:7999757</ref>

-	~~===THREE-DIMENSIONAL STRUCTURE OF PHOSPHOTRIESTERASE: AN ENZYME CAPABLE OF DETOXIFYING ORGANOPHOSPHATE NERVE AGENTS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7999757}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1pta]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA].~~	+

	==See Also==		==See Also==
	*[[Phosphotriesterase\|Phosphotriesterase]]		*[[Phosphotriesterase\|Phosphotriesterase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:007999757<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Brevundimonas diminuta]]		[[Category: Brevundimonas diminuta]]
	[[Category: Benning, M.]]		[[Category: Benning, M.]]
	[[Category: Holden, H M.]]		[[Category: Holden, H M.]]

OCA at 10:27, 20 October 2012

OCA — Sat, 20 Oct 2012 10:27:56 GMT

←Older revision		Revision as of 10:27, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1pta]] is a 1 chain structure ~~of [[Phosphotriesterase]]~~ with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA].	+	[[1pta]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTA OCA].

	==See Also==		==See Also==

OCA: Protected "1pta" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 02:12:29 GMT

Protected "1pta" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:12, 26 July 2012