1q57 - Revision history

OCA at 08:13, 14 February 2024

OCA — Wed, 14 Feb 2024 08:13:05 GMT

←Older revision		Revision as of 08:13, 14 February 2024
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	<StructureSection load='1q57' size='340' side='right'caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>		<StructureSection load='1q57' size='340' side='right'caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bpt7 Bpt7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.45Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">4 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10760 BPT7])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [https://pdbe.org/1q57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [https://www.ebi.ac.uk/pdbsum/1q57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q57 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [https://pdbe.org/1q57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [https://www.ebi.ac.uk/pdbsum/1q57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q57 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~PRIM_BPT7 PRIM_BPT7~~]~~] Synthesizes short RNA primers~~ for DNA replication. ~~Unwinds~~ the DNA at the replication ~~forks~~ and ~~generates single-stranded DNA~~ for both leading and lagging strand synthesis. ~~The primase~~ synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs.<ref>PMID:~~9096333~~</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref>	+	[https://www.uniprot.org/uniprot/HELIC_BPT7 HELIC_BPT7] ATP-dependent DNA helicase and primase essential for viral DNA replication and recombination (PubMed:21606333, PubMed:22977246, PubMed:32009150). The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis (PubMed:21606333, PubMed:22977246, PubMed:32009150). ATP or dTTP hydrolysis propels each helicase domain to translocate 2 nt per step sequentially along DNA (PubMed:30679383, PubMed:17604719). Mediates strand transfer when a joint molecule is available and participates in recombinational DNA repair through its role in strand exchange (PubMed:9096333, PubMed:8617248). Primase activity synthesizes short RNA primers at the sequence 5'-GTC-3' on the lagging strand that the polymerase elongates using dNTPs and providing the primase is still present (PubMed:6454135, PubMed:9139692).[HAMAP-Rule:MF_04154]<ref>PMID:17604719</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref> <ref>PMID:30679383</ref> <ref>PMID:32009150</ref> <ref>PMID:6454135</ref> <ref>PMID:8617248</ref> <ref>PMID:9096333</ref> <ref>PMID:9139692</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q57 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q57 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Within minutes after infecting Escherichia coli, bacteriophage T7 synthesizes many copies of its genomic DNA. The lynchpin of the T7 replication system is a bifunctional primase-helicase that unwinds duplex DNA at the replication fork while initiating the synthesis of Okazaki fragments on the lagging strand. We have determined a 3.45 A crystal structure of the T7 primase-helicase that shows an articulated arrangement of the primase and helicase sites. The crystallized primase-helicase is a heptamer with a crown-like shape, reflecting an intimate packing of helicase domains into a ring that is topped with loosely arrayed primase domains. This heptameric isoform can accommodate double-stranded DNA in its central channel, which nicely explains its recently described DNA remodeling activity. The double-jointed structure of the primase-helicase permits a free range of motion for the primase and helicase domains that suggests how the continuous unwinding of DNA at the replication fork can be periodically coupled to Okazaki fragment synthesis.
-
-	The crystal structure of the bifunctional primase-helicase of bacteriophage T7.,Toth EA, Li Y, Sawaya MR, Cheng Y, Ellenberger T Mol Cell. 2003 Nov;12(5):1113-23. PMID:14636571<ref>PMID:14636571</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1q57" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bpt7~~]]	+	[[Category: Escherichia phage T7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cheng, Y]]	+	[[Category: Cheng Y]]
-	[[Category: Ellenberger, T]]	+	[[Category: Ellenberger T]]
-	[[Category: Li, Y]]	+	[[Category: Li Y]]
-	[[Category: Sawaya~~, M R~~]]	+	[[Category: Sawaya MR]]
-	[[Category: Toth~~, E A]]~~	+	[[Category: Toth EA]]
-	~~[[Category: Dna replication]]~~	+
-	~~[[Category: Dntpase]]~~	+
-	~~[[Category: Helicase]]~~	+
-	~~[[Category: Primase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 15:20, 27 October 2021

OCA — Wed, 27 Oct 2021 15:20:07 GMT

←Older revision		Revision as of 15:20, 27 October 2021
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	<StructureSection load='1q57' size='340' side='right'caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>		<StructureSection load='1q57' size='340' side='right'caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</div></td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [~~http~~://pdbe.org/1q57 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1q57 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1q57 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [https://pdbe.org/1q57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [https://www.ebi.ac.uk/pdbsum/1q57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q57 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PRIM_BPT7 PRIM_BPT7]] Synthesizes short RNA primers for DNA replication. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs.<ref>PMID:9096333</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref>	+	[[https://www.uniprot.org/uniprot/PRIM_BPT7 PRIM_BPT7]] Synthesizes short RNA primers for DNA replication. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs.<ref>PMID:9096333</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:43, 9 December 2020

OCA — Wed, 09 Dec 2020 07:43:26 GMT

←Older revision		Revision as of 07:43, 9 December 2020
Line 1:		Line 1:

	==The Crystal Structure of the Bifunctional Primase-Helicase of Bacteriophage T7==		==The Crystal Structure of the Bifunctional Primase-Helicase of Bacteriophage T7==
-	<StructureSection load='1q57' size='340' side='right' caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>	+	<StructureSection load='1q57' size='340' side='right'caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [http://pdbe.org/1q57 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [http://www.ebi.ac.uk/pdbsum/1q57 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q57 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [http://pdbe.org/1q57 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [http://www.ebi.ac.uk/pdbsum/1q57 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q57 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1q57" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1q57" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Bpt7]]		[[Category: Bpt7]]
		+	[[Category: Large Structures]]
	[[Category: Cheng, Y]]		[[Category: Cheng, Y]]
	[[Category: Ellenberger, T]]		[[Category: Ellenberger, T]]

OCA at 07:51, 24 February 2018

OCA — Sat, 24 Feb 2018 07:51:25 GMT

←Older revision		Revision as of 07:51, 24 February 2018
Line 1:		Line 1:
		+
	==The Crystal Structure of the Bifunctional Primase-Helicase of Bacteriophage T7==		==The Crystal Structure of the Bifunctional Primase-Helicase of Bacteriophage T7==
	<StructureSection load='1q57' size='340' side='right' caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>		<StructureSection load='1q57' size='340' side='right' caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [http://pdbe.org/1q57 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [http://www.ebi.ac.uk/pdbsum/1q57 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [http://pdbe.org/1q57 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [http://www.ebi.ac.uk/pdbsum/1q57 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q57 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q5/1q57_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q5/1q57_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1q57" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1q57" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[RNA polymerase\|RNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:47, 8 February 2016

OCA — Mon, 08 Feb 2016 22:47:09 GMT

←Older revision		Revision as of 22:47, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q57 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:05, 10 September 2015

OCA — Thu, 10 Sep 2015 16:05:24 GMT

←Older revision		Revision as of 16:05, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1q57' size='340' side='right' caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>		<StructureSection load='1q57' size='340' side='right' caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_t7 Enterobacteria phage t7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 ~~Enterobacteria phage T7~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [http://www.ebi.ac.uk/pdbsum/1q57 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [http://pdbe.org/1q57 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [http://www.ebi.ac.uk/pdbsum/1q57 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1q57" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage t7~~]]	+	[[Category: Bpt7]]
	[[Category: Cheng, Y]]		[[Category: Cheng, Y]]
	[[Category: Ellenberger, T]]		[[Category: Ellenberger, T]]

OCA at 02:38, 25 December 2014

OCA — Thu, 25 Dec 2014 02:38:55 GMT

←Older revision		Revision as of 02:38, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [http://www.ebi.ac.uk/pdbsum/1q57 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [http://www.ebi.ac.uk/pdbsum/1q57 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRIM_BPT7 PRIM_BPT7]] Synthesizes short RNA primers for DNA replication. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs.<ref>PMID:9096333</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage t7]]		[[Category: Enterobacteria phage t7]]
-	[[Category: Cheng, Y.]]	+	[[Category: Cheng, Y]]
-	[[Category: Ellenberger, T.]]	+	[[Category: Ellenberger, T]]
-	[[Category: Li, Y.]]	+	[[Category: Li, Y]]
-	[[Category: Sawaya, M R.]]	+	[[Category: Sawaya, M R]]
-	[[Category: Toth, E A.]]	+	[[Category: Toth, E A]]
	[[Category: Dna replication]]		[[Category: Dna replication]]
	[[Category: Dntpase]]		[[Category: Dntpase]]

OCA at 19:13, 28 September 2014

OCA — Sun, 28 Sep 2014 19:13:42 GMT

←Older revision		Revision as of 19:13, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1q57.~~png~~\|~~left~~\|200px]]	+	==The Crystal Structure of the Bifunctional Primase-Helicase of Bacteriophage T7==
		+	<StructureSection load='1q57' size='340' side='right' caption='[[1q57]], [[Resolution\|resolution]] 3.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1q57]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q57 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e0j\|1e0j]], [[1cr0\|1cr0]], [[1nui\|1nui]], [[1e0k\|1e0k]], [[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q57 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q57 RCSB], [http://www.ebi.ac.uk/pdbsum/1q57 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q5/1q57_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Within minutes after infecting Escherichia coli, bacteriophage T7 synthesizes many copies of its genomic DNA. The lynchpin of the T7 replication system is a bifunctional primase-helicase that unwinds duplex DNA at the replication fork while initiating the synthesis of Okazaki fragments on the lagging strand. We have determined a 3.45 A crystal structure of the T7 primase-helicase that shows an articulated arrangement of the primase and helicase sites. The crystallized primase-helicase is a heptamer with a crown-like shape, reflecting an intimate packing of helicase domains into a ring that is topped with loosely arrayed primase domains. This heptameric isoform can accommodate double-stranded DNA in its central channel, which nicely explains its recently described DNA remodeling activity. The double-jointed structure of the primase-helicase permits a free range of motion for the primase and helicase domains that suggests how the continuous unwinding of DNA at the replication fork can be periodically coupled to Okazaki fragment synthesis.

-	~~{{STRUCTURE_1q57\| PDB=1q57 \| SCENE= }}~~	+	The crystal structure of the bifunctional primase-helicase of bacteriophage T7.,Toth EA, Li Y, Sawaya MR, Cheng Y, Ellenberger T Mol Cell. 2003 Nov;12(5):1113-23. PMID:14636571<ref>PMID:14636571</ref>

-	~~===The Crystal Structure~~ of the ~~Bifunctional Primase-Helicase of Bacteriophage T7===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14636571}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1q57]] is a 7 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA].~~	+

	==See Also==		==See Also==
	*[[RNA polymerase\|RNA polymerase]]		*[[RNA polymerase\|RNA polymerase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:014636571<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Enterobacteria phage t7]]		[[Category: Enterobacteria phage t7]]
	[[Category: Cheng, Y.]]		[[Category: Cheng, Y.]]

OCA at 11:44, 20 October 2012

OCA — Sat, 20 Oct 2012 11:44:03 GMT

←Older revision		Revision as of 11:44, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1q57]] is a 7 chain structure ~~of [[RNA polymerase]]~~ with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA].	+	[[1q57]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q57 OCA].

	==See Also==		==See Also==

OCA: Protected "1q57" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 06:25:01 GMT

Protected "1q57" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:25, 26 July 2012