1q6a - Revision history

OCA at 06:01, 17 April 2024

OCA — Wed, 17 Apr 2024 06:01:38 GMT

←Older revision		Revision as of 06:01, 17 April 2024
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	==Solution Structure of the C-terminal Domain of Thermosynechococcus elongatus KaiA (ThKaiA180C); Averaged Minimized Structure==		==Solution Structure of the C-terminal Domain of Thermosynechococcus elongatus KaiA (ThKaiA180C); Averaged Minimized Structure==
-	<StructureSection load='1q6a' size='340' side='right'caption='[[1q6a~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1q6a' size='340' side='right'caption='[[1q6a]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Theeb Theeb~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>KaiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~197221 THEEB])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [~~http~~://pdbe.org/1q6a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1q6a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1q6a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [https://pdbe.org/1q6a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [https://www.ebi.ac.uk/pdbsum/1q6a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q6a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~KAIA_THEEB KAIA_THEEB~~]~~] Component~~ of the KaiABC ~~clock protein~~ complex, which constitutes the main circadian regulator in cyanobacteria. ~~The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription~~, ~~possibly by acting on~~ the ~~state of chromosome compaction~~. In the complex, ~~it enhances~~ the ~~phosphorylation status of KaiC~~. ~~In contrast~~, the ~~presence of~~ KaiB ~~in the~~ complex ~~decreases the phosphorylation status~~ of KaiC, ~~suggesting that~~ KaiB ~~acts~~ by ~~antagonizing the interaction between~~ KaiA ~~and~~ KaiC. ~~A KaiA dimer is sufficient~~ to ~~enhance KaiC hexamer phosphorylation~~.	+	[https://www.uniprot.org/uniprot/KAIA_THEVB KAIA_THEVB] Key component of the KaiABC oscillator complex, which constitutes the main circadian regulator in cyanobacteria. Complex composition changes during the circadian cycle to control KaiC phosphorylation. KaiA stimulates KaiC autophosphorylation, while KaiB sequesters KaiA, leading to KaiC autodephosphorylation. KaiA binding to the KaiC CII domain during the subjective day yields KaiA(2-4):KaiC(6) complexes which stimulate KaiC autophosphorylation. Phospho-Ser-431 KaiC accumulation triggers binding of KaiB during the subjective night to form the KaiB(6):KaiC(6) complex, leading to changes in the output regulators CikA and SasA. KaiB(6):KaiC(6) formation exposes a site for KaiA binding on KaiB that sequesters KaiA from KaiC's CII domain, making the KaiC(6):KaiB(6):KaiA(12) complex resulting in KaiC autodephosphorylation. Complete dephosphorylation of KaiC leads to dissociation of KaiA(2):KaiB(1), completing 1 cycle of the Kai oscillator (By similarity). Formation of the KaiB:KaiC complex is promoted by KaiA, helping switch KaiC from its autophosphorylation to autodephosphatase function (PubMed:24112939).[UniProtKB:Q79PF6]<ref>PMID:24112939</ref> Binds oxidized quinones via the N-terminal PsR domain, allowing it to sense redox changes and possibly mediate clock input.[UniProtKB:Q79PF6]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q6a ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q6a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	KaiA is a two-domain circadian clock protein in cyanobacteria, acting as the positive element in a feedback loop that sustains the oscillation. The structure of the N-terminal domain of KaiA is that of a pseudo-receiver, similar to those of bacterial response regulators, which likely interacts with components of the clock-resetting pathway. The C-terminal domain of KaiA is highly conserved among cyanobacteria and enhances the autokinase activity of KaiC. Here we present the NMR structure of the C-terminal domain of KaiA from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. This domain adopts a novel all alpha-helical homodimeric structure. Several mutations known to affect the period of the circadian oscillator are shown to be located at an exposed groove near the dimer interface. This NMR structure and a 21-A-resolution electron microscopy structure of the hexameric KaiC particle allow us to postulate a mode of KaiA-KaiC interaction, in which KaiA binds a linker region connecting two globular KaiC domains.
-
-	NMR structure of the KaiC-interacting C-terminal domain of KaiA, a circadian clock protein: implications for KaiA-KaiC interaction.,Vakonakis I, Sun J, Wu T, Holzenburg A, Golden SS, LiWang AC Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1479-84. Epub 2004 Jan 28. PMID:14749515<ref>PMID:14749515</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1q6a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Theeb~~]]	+	[[Category: Thermosynechococcus vestitus BP-1]]
-	[[Category: Golden~~, S S~~]]	+	[[Category: Golden SS]]
-	[[Category: Holzenburg, A]]	+	[[Category: Holzenburg A]]
-	[[Category: LiWang~~, A C~~]]	+	[[Category: LiWang AC]]
-	[[Category: Sun, J]]	+	[[Category: Sun J]]
-	[[Category: Vakonakis, I]]	+	[[Category: Vakonakis I]]
-	~~[[Category: All alpha-helix protein]]~~	+
-	~~[[Category: Circadian clock protein]]~~	+
-	~~[[Category: Homodimer~~]]	+

OCA at 07:44, 9 December 2020

OCA — Wed, 09 Dec 2020 07:44:20 GMT

←Older revision		Revision as of 07:44, 9 December 2020
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	==Solution Structure of the C-terminal Domain of Thermosynechococcus elongatus KaiA (ThKaiA180C); Averaged Minimized Structure==		==Solution Structure of the C-terminal Domain of Thermosynechococcus elongatus KaiA (ThKaiA180C); Averaged Minimized Structure==
-	<StructureSection load='1q6a' size='340' side='right' caption='[[1q6a]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1q6a' size='340' side='right'caption='[[1q6a]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theeb Theeb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theeb Theeb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KaiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KaiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [http://pdbe.org/1q6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [http://www.ebi.ac.uk/pdbsum/1q6a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q6a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [http://pdbe.org/1q6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [http://www.ebi.ac.uk/pdbsum/1q6a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q6a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1q6a" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1q6a" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Circadian clock protein 3D structures\|Circadian clock protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Theeb]]		[[Category: Theeb]]
	[[Category: Golden, S S]]		[[Category: Golden, S S]]

OCA at 08:02, 24 February 2018

OCA — Sat, 24 Feb 2018 08:02:57 GMT

←Older revision		Revision as of 08:02, 24 February 2018
Line 1:		Line 1:
		+
	==Solution Structure of the C-terminal Domain of Thermosynechococcus elongatus KaiA (ThKaiA180C); Averaged Minimized Structure==		==Solution Structure of the C-terminal Domain of Thermosynechococcus elongatus KaiA (ThKaiA180C); Averaged Minimized Structure==
	<StructureSection load='1q6a' size='340' side='right' caption='[[1q6a]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1q6a' size='340' side='right' caption='[[1q6a]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theeb Theeb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>		<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theeb Theeb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KaiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KaiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [http://pdbe.org/1q6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [http://www.ebi.ac.uk/pdbsum/1q6a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [http://pdbe.org/1q6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [http://www.ebi.ac.uk/pdbsum/1q6a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q6a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q6/1q6a_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q6/1q6a_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 1q6a" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1q6a" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Circadian clock protein\|Circadian clock protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:42, 7 February 2016

OCA — Sun, 07 Feb 2016 08:42:52 GMT

←Older revision		Revision as of 08:42, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q6a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:44, 9 September 2015

OCA — Wed, 09 Sep 2015 23:44:38 GMT

←Older revision		Revision as of 23:44, 9 September 2015
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	<StructureSection load='1q6a' size='340' side='right' caption='[[1q6a]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1q6a' size='340' side='right' caption='[[1q6a]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermosynechococcus_elongatus_bp-1 Thermosynechococcus elongatus bp-1~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theeb Theeb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KaiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 ~~Thermosynechococcus elongatus BP-1~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KaiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [http://www.ebi.ac.uk/pdbsum/1q6a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [http://pdbe.org/1q6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [http://www.ebi.ac.uk/pdbsum/1q6a PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1q6a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermosynechococcus elongatus bp-1~~]]	+	[[Category: Theeb]]
	[[Category: Golden, S S]]		[[Category: Golden, S S]]
	[[Category: Holzenburg, A]]		[[Category: Holzenburg, A]]

OCA at 13:43, 24 December 2014

OCA — Wed, 24 Dec 2014 13:43:42 GMT

←Older revision		Revision as of 13:43, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_bp-1 Thermosynechococcus elongatus bp-1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>		<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_bp-1 Thermosynechococcus elongatus bp-1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KaiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 Thermosynechococcus elongatus BP-1])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KaiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 Thermosynechococcus elongatus BP-1])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [http://www.ebi.ac.uk/pdbsum/1q6a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [http://www.ebi.ac.uk/pdbsum/1q6a PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KAIA_THEEB KAIA_THEEB]] Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it enhances the phosphorylation status of KaiC. In contrast, the presence of KaiB in the complex decreases the phosphorylation status of KaiC, suggesting that KaiB acts by antagonizing the interaction between KaiA and KaiC. A KaiA dimer is sufficient to enhance KaiC hexamer phosphorylation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Thermosynechococcus elongatus bp-1]]		[[Category: Thermosynechococcus elongatus bp-1]]
-	[[Category: Golden, S S.]]	+	[[Category: Golden, S S]]
-	[[Category: Holzenburg, A.]]	+	[[Category: Holzenburg, A]]
-	[[Category: LiWang, A C.]]	+	[[Category: LiWang, A C]]
-	[[Category: Sun, J.]]	+	[[Category: Sun, J]]
-	[[Category: Vakonakis, I.]]	+	[[Category: Vakonakis, I]]
	[[Category: All alpha-helix protein]]		[[Category: All alpha-helix protein]]
	[[Category: Circadian clock protein]]		[[Category: Circadian clock protein]]
	[[Category: Homodimer]]		[[Category: Homodimer]]

OCA at 15:29, 29 September 2014

OCA — Mon, 29 Sep 2014 15:29:13 GMT

←Older revision		Revision as of 15:29, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1q6a.~~png\|left~~\|200px]]	+	==Solution Structure of the C-terminal Domain of Thermosynechococcus elongatus KaiA (ThKaiA180C); Averaged Minimized Structure==
		+	<StructureSection load='1q6a' size='340' side='right' caption='[[1q6a]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1q6a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_bp-1 Thermosynechococcus elongatus bp-1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q6A FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KaiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 Thermosynechococcus elongatus BP-1])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q6a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q6a RCSB], [http://www.ebi.ac.uk/pdbsum/1q6a PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q6/1q6a_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	KaiA is a two-domain circadian clock protein in cyanobacteria, acting as the positive element in a feedback loop that sustains the oscillation. The structure of the N-terminal domain of KaiA is that of a pseudo-receiver, similar to those of bacterial response regulators, which likely interacts with components of the clock-resetting pathway. The C-terminal domain of KaiA is highly conserved among cyanobacteria and enhances the autokinase activity of KaiC. Here we present the NMR structure of the C-terminal domain of KaiA from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. This domain adopts a novel all alpha-helical homodimeric structure. Several mutations known to affect the period of the circadian oscillator are shown to be located at an exposed groove near the dimer interface. This NMR structure and a 21-A-resolution electron microscopy structure of the hexameric KaiC particle allow us to postulate a mode of KaiA-KaiC interaction, in which KaiA binds a linker region connecting two globular KaiC domains.

-	~~{{STRUCTURE_1q6a\| PDB=1q6a \| SCENE= }}~~	+	NMR structure of the KaiC-interacting C-terminal domain of KaiA, a circadian clock protein: implications for KaiA-KaiC interaction.,Vakonakis I, Sun J, Wu T, Holzenburg A, Golden SS, LiWang AC Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1479-84. Epub 2004 Jan 28. PMID:14749515<ref>PMID:14749515</ref>

-	~~===Solution Structure~~ of the ~~C-terminal Domain~~ of ~~Thermosynechococcus elongatus KaiA (ThKaiA180C); Averaged Minimized Structure===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_14749515}}~~	+	==See Also==
-		+	*[[Circadian clock protein\|Circadian clock protein]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1q6a~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_bp-1 Thermosynechococcus elongatus bp-1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:014749515<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Thermosynechococcus elongatus bp-1]]		[[Category: Thermosynechococcus elongatus bp-1]]
	[[Category: Golden, S S.]]		[[Category: Golden, S S.]]

OCA: Protected "1q6a" [edit=sysop:move=sysop]

OCA — Wed, 02 Jan 2013 10:01:48 GMT

Protected "1q6a" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:01, 2 January 2013

OCA at 10:01, 2 January 2013

OCA — Wed, 02 Jan 2013 10:01:44 GMT

←Older revision		Revision as of 10:01, 2 January 2013
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-	{{Seed}}
	[[Image:1q6a.png\|left\|200px]]		[[Image:1q6a.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1q6a", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1q6a\| PDB=1q6a \| SCENE= }}		{{STRUCTURE_1q6a\| PDB=1q6a \| SCENE= }}

	===Solution Structure of the C-terminal Domain of Thermosynechococcus elongatus KaiA (ThKaiA180C); Averaged Minimized Structure===		===Solution Structure of the C-terminal Domain of Thermosynechococcus elongatus KaiA (ThKaiA180C); Averaged Minimized Structure===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_14749515}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 14749515 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_14749515}}		{{ABSTRACT_PUBMED_14749515}}

	==About this Structure==		==About this Structure==
-	~~1Q6A~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Bacteria Bacteria~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA].	+	[[1q6a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_bp-1 Thermosynechococcus elongatus bp-1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14749515~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014749515</ref><references group="xtra"/>
-	[[Category: ~~Bacteria~~]]	+	[[Category: Thermosynechococcus elongatus bp-1]]
	[[Category: Golden, S S.]]		[[Category: Golden, S S.]]
	[[Category: Holzenburg, A.]]		[[Category: Holzenburg, A.]]
Line 31:		Line 19:
	[[Category: Vakonakis, I.]]		[[Category: Vakonakis, I.]]
	[[Category: All alpha-helix protein]]		[[Category: All alpha-helix protein]]
		+	[[Category: Circadian clock protein]]
	[[Category: Homodimer]]		[[Category: Homodimer]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:39:28 2009''

OCA at 10:39, 16 February 2009

OCA — Mon, 16 Feb 2009 10:39:28 GMT

←Older revision		Revision as of 10:39, 16 February 2009
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	==About this Structure==		==About this Structure==
-	1Q6A is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA].	+	1Q6A is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q6A OCA].

	==Reference==		==Reference==
-	NMR structure of the KaiC-interacting C-terminal domain of KaiA, a circadian clock protein: implications for KaiA-KaiC interaction., Vakonakis I, Sun J, Wu T, Holzenburg A, Golden SS, LiWang AC, Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1479-84. Epub 2004 Jan 28. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14749515 14749515]~~	+	<ref group="xtra">PMID:14749515</ref><references group="xtra"/>
	[[Category: Bacteria]]		[[Category: Bacteria]]
-	[[Category: Single protein]]
	[[Category: Golden, S S.]]		[[Category: Golden, S S.]]
	[[Category: Holzenburg, A.]]		[[Category: Holzenburg, A.]]
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	[[Category: Homodimer]]		[[Category: Homodimer]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 14~~:55:~~34 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:39:28 2009''