1q71 - Revision history

OCA at 07:15, 14 September 2022

OCA — Wed, 14 Sep 2022 07:15:28 GMT

←Older revision		Revision as of 07:15, 14 September 2022
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	==The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone==		==The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone==
-	<StructureSection load='1q71' size='340' side='right'caption='[[1q71~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1q71' size='340' side='right'caption='[[1q71]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q71]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1Q71 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q71]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q71 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[1hg6\|1hg6]]~~<~~/div></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PRD_000184:Microcin+J25'>PRD_000184</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">mcjA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [https://pdbe.org/1q71 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [https://www.ebi.ac.uk/pdbsum/1q71 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q71 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [~~http~~://pdbe.org/1q71 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1q71 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1q71 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MCJA_ECOLX MCJA_ECOLX]] Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). May inhibit transcription by binding in RNAP secondary channel and blocking nucleotide substrates access to the catalytic center. Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains. Also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and disruption of the subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption. Induces bacterial filamentation in susceptible cells in a non-SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins.<ref>PMID:11731133</ref> <ref>PMID:11443089</ref> <ref>PMID:12401787</ref>	+	[[https://www.uniprot.org/uniprot/MCJA_ECOLX MCJA_ECOLX]] Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). May inhibit transcription by binding in RNAP secondary channel and blocking nucleotide substrates access to the catalytic center. Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains. Also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and disruption of the subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption. Induces bacterial filamentation in susceptible cells in a non-SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins.<ref>PMID:11731133</ref> <ref>PMID:11443089</ref> <ref>PMID:12401787</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Clark, R]]	+	[[Category: Clark R]]
-	[[Category: Craik~~, D J~~]]	+	[[Category: Craik DJ]]
-	[[Category: Daly~~, N L~~]]	+	[[Category: Daly NL]]
-	[[Category: Goransson, U]]	+	[[Category: Goransson U]]
-	[[Category: Jones, A]]	+	[[Category: Jones A]]
-	[[Category: Rosengren~~, K J]]~~	+	[[Category: Rosengren KJ]]
-	~~[[Category: Antibiotic]]~~	+
-	~~[[Category: Antimicrobial peptide]]~~	+
-	~~[[Category: Antimicrobial protein]]~~	+
-	~~[[Category: Mccj25]]~~	+
-	~~[[Category: Microcin j25]]~~	+
-	~~[[Category: Sidechain-to-backbone link~~]]	+

OCA at 07:44, 9 December 2020

OCA — Wed, 09 Dec 2020 07:44:59 GMT

←Older revision		Revision as of 07:44, 9 December 2020
Line 1:		Line 1:

	==The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone==		==The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone==
-	<StructureSection load='1q71' size='340' side='right' caption='[[1q71]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1q71' size='340' side='right'caption='[[1q71]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q71]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Q71 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q71]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Q71 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hg6\|1hg6]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hg6\|1hg6]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcjA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcjA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [http://pdbe.org/1q71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [http://www.ebi.ac.uk/pdbsum/1q71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q71 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [http://pdbe.org/1q71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [http://www.ebi.ac.uk/pdbsum/1q71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q71 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Clark, R]]		[[Category: Clark, R]]
	[[Category: Craik, D J]]		[[Category: Craik, D J]]

OCA at 08:23, 24 February 2018

OCA — Sat, 24 Feb 2018 08:23:33 GMT

←Older revision		Revision as of 08:23, 24 February 2018
Line 1:		Line 1:
		+
	==The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone==		==The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone==
	<StructureSection load='1q71' size='340' side='right' caption='[[1q71]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1q71' size='340' side='right' caption='[[1q71]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hg6\|1hg6]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hg6\|1hg6]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcjA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcjA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [http://pdbe.org/1q71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [http://www.ebi.ac.uk/pdbsum/1q71 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [http://pdbe.org/1q71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [http://www.ebi.ac.uk/pdbsum/1q71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q71 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 12:56, 10 September 2015

OCA — Thu, 10 Sep 2015 12:56:03 GMT

←Older revision		Revision as of 12:56, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1q71' size='340' side='right' caption='[[1q71]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1q71' size='340' side='right' caption='[[1q71]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q71]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q71 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q71]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q71 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hg6\|1hg6]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hg6\|1hg6]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcjA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcjA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [http://www.ebi.ac.uk/pdbsum/1q71 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [http://pdbe.org/1q71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [http://www.ebi.ac.uk/pdbsum/1q71 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~MCJA_ECOLI MCJA_ECOLI~~]] Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). May inhibit transcription by binding in RNAP secondary channel and blocking nucleotide substrates access to the catalytic center. Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains. Also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and disruption of the subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption. Induces bacterial filamentation in susceptible cells in a non-SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins.<ref>PMID:11731133</ref> <ref>PMID:11443089</ref> <ref>PMID:12401787</ref>	+	[[http://www.uniprot.org/uniprot/MCJA_ECOLX MCJA_ECOLX]] Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). May inhibit transcription by binding in RNAP secondary channel and blocking nucleotide substrates access to the catalytic center. Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains. Also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and disruption of the subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption. Induces bacterial filamentation in susceptible cells in a non-SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins.<ref>PMID:11731133</ref> <ref>PMID:11443089</ref> <ref>PMID:12401787</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1q71" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Clark, R]]		[[Category: Clark, R]]
	[[Category: Craik, D J]]		[[Category: Craik, D J]]

OCA at 00:19, 25 December 2014

OCA — Thu, 25 Dec 2014 00:19:20 GMT

←Older revision		Revision as of 00:19, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [http://www.ebi.ac.uk/pdbsum/1q71 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [http://www.ebi.ac.uk/pdbsum/1q71 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MCJA_ECOLI MCJA_ECOLI]] Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). May inhibit transcription by binding in RNAP secondary channel and blocking nucleotide substrates access to the catalytic center. Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains. Also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and disruption of the subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption. Induces bacterial filamentation in susceptible cells in a non-SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins.<ref>PMID:11731133</ref> <ref>PMID:11443089</ref> <ref>PMID:12401787</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:42, 22 December 2014

OCA — Mon, 22 Dec 2014 06:42:41 GMT

←Older revision		Revision as of 06:42, 22 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1q71\| PDB=1q71 \| SCENE= }}~~	+	==The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone==
-	===The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone===	+	<StructureSection load='1q71' size='340' side='right' caption='[[1q71]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_14531690}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1q71]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q71 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hg6\|1hg6]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcjA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q71 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q71 RCSB], [http://www.ebi.ac.uk/pdbsum/1q71 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microcin J25 is a 21 amino acid bacterial peptide that has potent antibacterial activity against Gram-negative bacteria, resulting from its interaction with RNA polymerase. The peptide was previously proposed to have a head-to-tail cyclized peptide backbone and a tight globular structure (Blond, A., Peduzzi, J., Goulard, C., Chiuchiolo, M. J., Barthelemy, M., Prigent, Y., Salomon, R. A., Farias, R. N., Moreno, F. & Rebuffat, S. Eur. J. Biochem. 1999, 259, 747-755). It exhibits remarkable thermal stability for a peptide of its size lacking disulfide bonds and in part this was previously proposed to derive from its macrocyclic structure. We show here that in fact the peptide does not have a head-to-tail cyclic structure but rather a side chain to backbone cyclization between Glu8 and the N-terminus. This creates an embedded ring that is threaded by the C-terminal tail of the molecule, forming a noose-like feature. The three-dimensional structure deduced from NMR data suggests that slippage of the noose is prevented by two aromatic residues flanking the embedded ring. Unthreading does not occur even when the molecule is enzymatically digested with thermolysin. The new structural interpretation fully accounts for previously reported NMR and biophysical data and is consistent with the remarkable stability of this potent antimicrobial peptide.

-	~~==Function==~~	+	Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone.,Rosengren KJ, Clark RJ, Daly NL, Goransson U, Jones A, Craik DJ J Am Chem Soc. 2003 Oct 15;125(41):12464-74. PMID:14531690<ref>PMID:14531690</ref>
-	~~[[http://www.uniprot.org/uniprot/MCJA_ECOLI MCJA_ECOLI]] Peptide antibiotic that functions through inhibition of the bacterial DNA~~-~~dependent RNA polymerase (RNAP). May inhibit transcription by binding in RNAP secondary channel~~ and ~~blocking nucleotide substrates access~~ to ~~the catalytic center~~. ~~Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae~~, ~~including several pathogenic E.coli~~, ~~Salmonella and Shigella strains. Also acts on the cytoplasmic membrane of Salmonella newport~~, ~~producing alteration of membrane permeability and disruption of the subsequent gradient dissipation~~, ~~which inhibits several processes essential for cell viability~~, ~~such as oxygen consumption~~. ~~Induces bacterial filamentation in susceptible cells in a non~~-~~SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins~~.~~<ref>~~PMID:~~11731133</ref> <ref>PMID:11443089</ref>~~ <ref>PMID:~~12401787~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[1q71]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:014531690</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Clark, R.]]	+	[[Category: Clark, R]]
-	[[Category: Craik, D J.]]	+	[[Category: Craik, D J]]
-	[[Category: Daly, N L.]]	+	[[Category: Daly, N L]]
-	[[Category: Goransson, U.]]	+	[[Category: Goransson, U]]
-	[[Category: Jones, A.]]	+	[[Category: Jones, A]]
-	[[Category: Rosengren, K J.]]	+	[[Category: Rosengren, K J]]
	[[Category: Antibiotic]]		[[Category: Antibiotic]]
	[[Category: Antimicrobial peptide]]		[[Category: Antimicrobial peptide]]

OCA at 10:49, 16 April 2014

OCA — Wed, 16 Apr 2014 10:49:04 GMT

←Older revision		Revision as of 10:49, 16 April 2014
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-	[[Image:1q71.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_1q71", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1q71\| PDB=1q71 \| SCENE= }}		{{STRUCTURE_1q71\| PDB=1q71 \| SCENE= }}
-
	===The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone===		===The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone===
		+	{{ABSTRACT_PUBMED_14531690}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/MCJA_ECOLI MCJA_ECOLI]] Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). May inhibit transcription by binding in RNAP secondary channel and blocking nucleotide substrates access to the catalytic center. Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains. Also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and disruption of the subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption. Induces bacterial filamentation in susceptible cells in a non-SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins.<ref>PMID:11731133</ref> <ref>PMID:11443089</ref> <ref>PMID:12401787</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_14531690}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~), ~~where 14531690 is~~ the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_14531690}}~~	+

	==About this Structure==		==About this Structure==
Line 22:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:014531690</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014531690</ref><references group="xtra"/><references/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Clark, R.]]		[[Category: Clark, R.]]

OCA: Protected "1q71" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2011 05:03:37 GMT

Protected "1q71" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:03, 27 July 2011

OCA at 05:03, 27 July 2011

OCA — Wed, 27 Jul 2011 05:03:35 GMT

←Older revision		Revision as of 05:03, 27 July 2011
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1q71.png\|left\|200px]]		[[Image:1q71.png\|left\|200px]]

Line 20:		Line 19:

	==About this Structure==		==About this Structure==
-	~~1Q71 is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA].	+	[[1q71]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA].

	==Reference==		==Reference==
-	Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone., Rosengren KJ, Clark RJ, Daly NL, Goransson U, Jones A, Craik DJ, J Am Chem Soc. 2003 Oct 15;125(41):12464-74. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~14531690 14531690]~~	+	<ref group="xtra">PMID:014531690</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Clark, R.]]		[[Category: Clark, R.]]
	[[Category: Craik, D J.]]		[[Category: Craik, D J.]]
Line 32:		Line 30:
	[[Category: Jones, A.]]		[[Category: Jones, A.]]
	[[Category: Rosengren, K J.]]		[[Category: Rosengren, K J.]]
		+	[[Category: Antibiotic]]
	[[Category: Antimicrobial peptide]]		[[Category: Antimicrobial peptide]]
		+	[[Category: Antimicrobial protein]]
	[[Category: Mccj25]]		[[Category: Mccj25]]
	[[Category: Microcin j25]]		[[Category: Microcin j25]]
-	[[Category: Nmr]]
	[[Category: Sidechain-to-backbone link]]		[[Category: Sidechain-to-backbone link]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:21:04 2008''

OCA at 01:21, 28 July 2008

OCA — Mon, 28 Jul 2008 01:21:09 GMT

←Older revision		Revision as of 01:21, 28 July 2008
Line 1:		Line 1:
-	[[Image:1q71.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1q71.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1q71\| PDB=1q71 \| SCENE= }}		{{STRUCTURE_1q71\| PDB=1q71 \| SCENE= }}

-	~~'''~~The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone~~'''~~	+	===The structure of microcin J25 is a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone===


-	~~==Overview==~~	+	<!--
-	~~Microcin J25 is a 21 amino acid bacterial peptide that has potent antibacterial activity against Gram-negative bacteria, resulting from its interaction with RNA polymerase.~~ The ~~peptide was previously proposed to have a head-to-tail cyclized peptide backbone and a tight globular structure (Blond~~, A., Peduzzi, J., Goulard, C., Chiuchiolo, M. J., Barthelemy, M., Prigent, Y., Salomon, R. A., Farias, R. N., Moreno, F. & Rebuffat, S. Eur. J. Biochem. 1999, 259, 747-755). It exhibits remarkable thermal stability for a peptide of its size lacking disulfide bonds and in part this was previously proposed to derive from its macrocyclic structure. We show here that in fact the ~~peptide does not have a head-~~to~~-tail cyclic structure but rather a side chain to backbone cyclization between Glu8 and~~ the ~~N-terminus~~. ~~This creates an embedded ring that~~ is ~~threaded by~~ the C-~~terminal tail of the molecule, forming a noose~~-like feature. The three-dimensional structure deduced from NMR data suggests that slippage of the noose is prevented by two aromatic residues flanking the embedded ring. Unthreading does not occur even when the molecule is enzymatically digested with thermolysin. The new structural interpretation fully accounts for previously reported NMR and biophysical data and is consistent with the remarkable stability of this potent antimicrobial peptide.	+	The line below this paragraph, {{ABSTRACT_PUBMED_14531690}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 14531690 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_14531690}}

	==About this Structure==		==About this Structure==
-	1Q71 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA].	+	1Q71 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q71 OCA].

	==Reference==		==Reference==
Line 33:		Line 37:
	[[Category: Nmr]]		[[Category: Nmr]]
	[[Category: Sidechain-to-backbone link]]		[[Category: Sidechain-to-backbone link]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 05~~:56:52 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:21:04 2008''