1qk8 - Revision history

OCA at 06:04, 17 April 2024

OCA — Wed, 17 Apr 2024 06:04:05 GMT

←Older revision		Revision as of 06:04, 17 April 2024
Line 3:		Line 3:
	<StructureSection load='1qk8' size='340' side='right'caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1qk8' size='340' side='right'caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Crifa Crifa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [https://pdbe.org/1qk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [https://www.ebi.ac.uk/pdbsum/1qk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qk8 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [https://pdbe.org/1qk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [https://www.ebi.ac.uk/pdbsum/1qk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qk8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O96438_CRIFA O96438_CRIFA]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qk8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qk8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Tryparedoxin-I is a recently discovered thiol-disulfide oxidoreductase involved in the regulation of oxidative stress in parasitic trypanosomatids. The crystal structure of recombinant Crithidia fasciculata tryparedoxin-I in the oxidized state has been determined using multi-wavelength anomalous dispersion methods applied to a selenomethionyl derivative. The model comprises residues 3 to 145 with 236 water molecules and has been refined using all data between a 19- and 1.4-A resolution to an R-factor and R-free of 19.1 and 22.3%, respectively. Despite sharing only about 20% sequence identity, tryparedoxin-I presents a five-stranded twisted beta-sheet and two elements of helical structure in the same type of fold as displayed by thioredoxin, the archetypal thiol-disulfide oxidoreductase. However, the relationship of secondary structure with the linear amino acid sequences is different for each protein, producing a distinctive topology. The beta-sheet core is extended in the trypanosomatid protein with an N-terminal beta-hairpin. There are also differences in the content and orientation of helical elements of secondary structure positioned at the surface of the proteins, which leads to different shapes and charge distributions between human thioredoxin and tryparedoxin-I. A right-handed redox-active disulfide is formed between Cys-40 and Cys-43 at the N-terminal region of a distorted alpha-helix (alpha1). Cys-40 is solvent-accessible, and Cys-43 is positioned in a hydrophilic cavity. Three C-H...O hydrogen bonds donated from two proline residues serve to stabilize the disulfide-carrying helix and support the correct alignment of active site residues. The accurate model for tryparedoxin-I allows for comparisons with the family of thiol-disulfide oxidoreductases and provides a template for the discovery or design of selective inhibitors of hydroperoxide metabolism in trypanosomes. Such inhibitors are sought as potential therapies against a range of human pathogens.
-
-	The high resolution crystal structure of recombinant Crithidia fasciculata tryparedoxin-I.,Alphey MS, Leonard GA, Gourley DG, Tetaud E, Fairlamb AH, Hunter WN J Biol Chem. 1999 Sep 3;274(36):25613-22. PMID:10464297<ref>PMID:10464297</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1qk8" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Crifa]]
-	[[Category: Large Structures]]
-	[[Category: Alphey, M S]]
-	[[Category: Hunter, W N]]
-	[[Category: Anomalous dispersion]]
	[[Category: Crithidia fasciculata]]		[[Category: Crithidia fasciculata]]
-	[[Category: ~~Oxidative stress~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Oxidoreductase~~]]	+	[[Category: Alphey MS]]
-	[[Category: ~~Thioredoxin]]~~	+	[[Category: Hunter WN]]
-	~~[[Category: Trypanosome]]~~	+
-	~~[[Category: Tryparedoxin~~]]	+

OCA at 09:58, 15 September 2021

OCA — Wed, 15 Sep 2021 09:58:19 GMT

←Older revision		Revision as of 09:58, 15 September 2021
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	<StructureSection load='1qk8' size='340' side='right'caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1qk8' size='340' side='right'caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Crifa Crifa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Crifa Crifa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [~~http~~://pdbe.org/1qk8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1qk8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1qk8 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [https://pdbe.org/1qk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [https://www.ebi.ac.uk/pdbsum/1qk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qk8 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 10:09, 27 November 2019

OCA — Wed, 27 Nov 2019 10:09:09 GMT

←Older revision		Revision as of 10:09, 27 November 2019
Line 1:		Line 1:

	==TRYPAREDOXIN-I FROM CRITHIDIA FASCICULATA==		==TRYPAREDOXIN-I FROM CRITHIDIA FASCICULATA==
-	<StructureSection load='1qk8' size='340' side='right' caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='1qk8' size='340' side='right'caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crifa Crifa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crifa Crifa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Crifa]]		[[Category: Crifa]]
		+	[[Category: Large Structures]]
	[[Category: Alphey, M S]]		[[Category: Alphey, M S]]
	[[Category: Hunter, W N]]		[[Category: Hunter, W N]]

OCA at 08:09, 24 February 2018

OCA — Sat, 24 Feb 2018 08:09:40 GMT

←Older revision		Revision as of 08:09, 24 February 2018
Line 1:		Line 1:
		+
	==TRYPAREDOXIN-I FROM CRITHIDIA FASCICULATA==		==TRYPAREDOXIN-I FROM CRITHIDIA FASCICULATA==
	<StructureSection load='1qk8' size='340' side='right' caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1qk8' size='340' side='right' caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crifa Crifa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crifa Crifa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [http://pdbe.org/1qk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [http://www.ebi.ac.uk/pdbsum/1qk8 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [http://pdbe.org/1qk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [http://www.ebi.ac.uk/pdbsum/1qk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qk8 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 9:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qk/1qk8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qk/1qk8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 00:50, 8 February 2016

OCA — Mon, 08 Feb 2016 00:50:20 GMT

←Older revision		Revision as of 00:50, 8 February 2016
Line 13:		Line 13:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qk8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:20, 10 September 2015

OCA — Thu, 10 Sep 2015 06:20:44 GMT

←Older revision		Revision as of 06:20, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1qk8' size='340' side='right' caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1qk8' size='340' side='right' caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Crithidia_fasciculata Crithidia fasciculata~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crifa Crifa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [http://www.ebi.ac.uk/pdbsum/1qk8 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [http://pdbe.org/1qk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [http://www.ebi.ac.uk/pdbsum/1qk8 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1qk8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Crithidia fasciculata~~]]	+	[[Category: Crifa]]
	[[Category: Alphey, M S]]		[[Category: Alphey, M S]]
	[[Category: Hunter, W N]]		[[Category: Hunter, W N]]
	[[Category: Anomalous dispersion]]		[[Category: Anomalous dispersion]]
		+	[[Category: Crithidia fasciculata]]
	[[Category: Oxidative stress]]		[[Category: Oxidative stress]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 08:03, 6 January 2015

OCA — Tue, 06 Jan 2015 08:03:50 GMT

←Older revision		Revision as of 08:03, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [http://www.ebi.ac.uk/pdbsum/1qk8 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [http://www.ebi.ac.uk/pdbsum/1qk8 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Crithidia fasciculata]]		[[Category: Crithidia fasciculata]]
-	[[Category: Alphey, M S.]]	+	[[Category: Alphey, M S]]
-	[[Category: Hunter, W N.]]	+	[[Category: Hunter, W N]]
	[[Category: Anomalous dispersion]]		[[Category: Anomalous dispersion]]
-	[[Category: Crithidia fasciculata]]
	[[Category: Oxidative stress]]		[[Category: Oxidative stress]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 15:33, 29 September 2014

OCA — Mon, 29 Sep 2014 15:33:28 GMT

←Older revision		Revision as of 15:33, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1qk8.~~png\|left~~\|200px]]	+	==TRYPAREDOXIN-I FROM CRITHIDIA FASCICULATA==
		+	<StructureSection load='1qk8' size='340' side='right' caption='[[1qk8]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1qk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qk8 RCSB], [http://www.ebi.ac.uk/pdbsum/1qk8 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qk/1qk8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tryparedoxin-I is a recently discovered thiol-disulfide oxidoreductase involved in the regulation of oxidative stress in parasitic trypanosomatids. The crystal structure of recombinant Crithidia fasciculata tryparedoxin-I in the oxidized state has been determined using multi-wavelength anomalous dispersion methods applied to a selenomethionyl derivative. The model comprises residues 3 to 145 with 236 water molecules and has been refined using all data between a 19- and 1.4-A resolution to an R-factor and R-free of 19.1 and 22.3%, respectively. Despite sharing only about 20% sequence identity, tryparedoxin-I presents a five-stranded twisted beta-sheet and two elements of helical structure in the same type of fold as displayed by thioredoxin, the archetypal thiol-disulfide oxidoreductase. However, the relationship of secondary structure with the linear amino acid sequences is different for each protein, producing a distinctive topology. The beta-sheet core is extended in the trypanosomatid protein with an N-terminal beta-hairpin. There are also differences in the content and orientation of helical elements of secondary structure positioned at the surface of the proteins, which leads to different shapes and charge distributions between human thioredoxin and tryparedoxin-I. A right-handed redox-active disulfide is formed between Cys-40 and Cys-43 at the N-terminal region of a distorted alpha-helix (alpha1). Cys-40 is solvent-accessible, and Cys-43 is positioned in a hydrophilic cavity. Three C-H...O hydrogen bonds donated from two proline residues serve to stabilize the disulfide-carrying helix and support the correct alignment of active site residues. The accurate model for tryparedoxin-I allows for comparisons with the family of thiol-disulfide oxidoreductases and provides a template for the discovery or design of selective inhibitors of hydroperoxide metabolism in trypanosomes. Such inhibitors are sought as potential therapies against a range of human pathogens.

-	~~{{STRUCTURE_1qk8\| PDB=1qk8 \| SCENE= }}~~	+	The high resolution crystal structure of recombinant Crithidia fasciculata tryparedoxin-I.,Alphey MS, Leonard GA, Gourley DG, Tetaud E, Fairlamb AH, Hunter WN J Biol Chem. 1999 Sep 3;274(36):25613-22. PMID:10464297<ref>PMID:10464297</ref>

-	~~===TRYPAREDOXIN-I FROM CRITHIDIA FASCICULATA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10464297}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1qk8]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Crithidia_fasciculata Crithidia fasciculata]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:010464297<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Crithidia fasciculata]]		[[Category: Crithidia fasciculata]]
	[[Category: Alphey, M S.]]		[[Category: Alphey, M S.]]

OCA: Protected "1qk8" [edit=sysop:move=sysop]

OCA — Wed, 02 Jan 2013 10:11:06 GMT

Protected "1qk8" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:11, 2 January 2013

OCA at 10:11, 2 January 2013

OCA — Wed, 02 Jan 2013 10:11:04 GMT

←Older revision		Revision as of 10:11, 2 January 2013
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-	{{Seed}}
	[[Image:1qk8.png\|left\|200px]]		[[Image:1qk8.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1qk8", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1qk8\| PDB=1qk8 \| SCENE= }}		{{STRUCTURE_1qk8\| PDB=1qk8 \| SCENE= }}

	===TRYPAREDOXIN-I FROM CRITHIDIA FASCICULATA===		===TRYPAREDOXIN-I FROM CRITHIDIA FASCICULATA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10464297}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10464297 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10464297}}		{{ABSTRACT_PUBMED_10464297}}

	==About this Structure==		==About this Structure==
-	~~1QK8~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA].	+	[[1qk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK8 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10464297~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010464297</ref><references group="xtra"/>
	[[Category: Crithidia fasciculata]]		[[Category: Crithidia fasciculata]]
	[[Category: Alphey, M S.]]		[[Category: Alphey, M S.]]
Line 34:		Line 22:
	[[Category: Trypanosome]]		[[Category: Trypanosome]]
	[[Category: Tryparedoxin]]		[[Category: Tryparedoxin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:36:27 2009''