1qpg - Revision history

OCA at 08:15, 14 February 2024

OCA — Wed, 14 Feb 2024 08:15:50 GMT

←Older revision		Revision as of 08:15, 14 February 2024
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	<StructureSection load='1qpg' size='340' side='right'caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1qpg' size='340' side='right'caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QPG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QPG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=3PG:3~~-~~PHOSPHOGLYCERIC+ACID'>3PG</scene>~~, ~~<scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase]~~, ~~with EC number [https~~:~~//www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [https://pdbe.org/1qpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [https://www.ebi.ac.uk/pdbsum/1qpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qpg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [https://pdbe.org/1qpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [https://www.ebi.ac.uk/pdbsum/1qpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qpg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PGK_YEAST PGK_YEAST]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qpg ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qpg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The structure of a ternary complex of the R65Q mutant of yeast 3-phosphoglycerate kinase (PGK) with magnesium 5'-adenylylimidodiphosphate (Mg-AMP-PNP) and 3-phospho-D-glycerate (3-PG) has been determined by X-ray crystallography to 2.4 angstrom resolution. The structure was solved by single isomorphous replacement, anamalous scattering, and solvent flattening and has been refined to an R-factor of 0.185, with rms deviations from ideal bond distance and angles of 0.009 angstrom and 1.78 degrees, respectively. PGK consists of two domains, with the 3-PG bound to a "basic patch" of residues from the N-terminal domain and the Mg-AMP-PNP interacting with residues from the C-terminal domain. The two ligands are separated by approximately 11 angstrom across the interdomain cleft. The model of the R65Q mutant of yeast PGK is very similar to the structures of PGK isolated from horse, pig, and Bacillus stearothermophilus (rms deviations between equivalent alpha-carbons in the individual domains < 1.0 angstrom) but exhibits substantial variations with a previously reported yeast structure (rms deviations between equivalent alpha-carbons in the individual domains of 2.9-3.2 angstrom). The most significant tertiary structural differences among the yeast R65Q, equine, porcine, and B. stearothermophilus PGK structures occur in the relative orientations of the two domains. However, the relationships between the observed conformations of PGK are inconsistent with a "hinge-bending" behavior that would close the interdomain cleft. It is proposed that the available structural and biochemical data on PGK may indicate that the basic patch primarily represents the site of anion activation and not the catalytically active binding site for 3-PG.
-
-	Structure of the R65Q mutant of yeast 3-phosphoglycerate kinase complexed with Mg-AMP-PNP and 3-phospho-D-glycerate.,McPhillips TM, Hsu BT, Sherman MA, Mas MT, Rees DC Biochemistry. 1996 Apr 2;35(13):4118-27. PMID:8672447<ref>PMID:8672447</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1qpg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Phosphoglycerate kinase 3D structures\|Phosphoglycerate kinase 3D structures]]		*[[Phosphoglycerate kinase 3D structures\|Phosphoglycerate kinase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Phosphoglycerate kinase~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Hsu~~, B T~~]]	+	[[Category: Hsu BT]]
-	[[Category: Mas~~, M T~~]]	+	[[Category: Mas MT]]
-	[[Category: Mcphillips~~, T M~~]]	+	[[Category: Mcphillips TM]]
-	[[Category: Rees~~, D C~~]]	+	[[Category: Rees DC]]
-	[[Category: Sherman~~, M A]]~~	+	[[Category: Sherman MA]]
-	~~[[Category: Acetylation]]~~	+
-	~~[[Category: Glycolysis]]~~	+
-	~~[[Category: Kinase~~]]	+

OCA at 10:48, 19 May 2021

OCA — Wed, 19 May 2021 10:48:20 GMT

←Older revision		Revision as of 10:48, 19 May 2021
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	<StructureSection load='1qpg' size='340' side='right'caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1qpg' size='340' side='right'caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1QPG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QPG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [~~http~~://pdbe.org/1qpg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1qpg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1qpg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [https://pdbe.org/1qpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [https://www.ebi.ac.uk/pdbsum/1qpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qpg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Phosphoglycerate ~~Kinase~~\|Phosphoglycerate ~~Kinase~~]]	+	*[[Phosphoglycerate kinase 3D structures\|Phosphoglycerate kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:59, 17 July 2019

OCA — Wed, 17 Jul 2019 10:59:51 GMT

←Older revision		Revision as of 10:59, 17 July 2019
Line 1:		Line 1:

	==3-PHOSPHOGLYCERATE KINASE, MUTATION R65Q==		==3-PHOSPHOGLYCERATE KINASE, MUTATION R65Q==
-	<StructureSection load='1qpg' size='340' side='right' caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='1qpg' size='340' side='right'caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QPG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QPG FirstGlance]. <br>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Phosphoglycerate kinase]]		[[Category: Phosphoglycerate kinase]]
	[[Category: Hsu, B T]]		[[Category: Hsu, B T]]

OCA at 07:51, 28 February 2018

OCA — Wed, 28 Feb 2018 07:51:09 GMT

←Older revision		Revision as of 07:51, 28 February 2018
Line 1:		Line 1:
		+
	==3-PHOSPHOGLYCERATE KINASE, MUTATION R65Q==		==3-PHOSPHOGLYCERATE KINASE, MUTATION R65Q==
	<StructureSection load='1qpg' size='340' side='right' caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1qpg' size='340' side='right' caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [http://pdbe.org/1qpg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [http://www.ebi.ac.uk/pdbsum/1qpg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [http://pdbe.org/1qpg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [http://www.ebi.ac.uk/pdbsum/1qpg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qpg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/1qpg_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/1qpg_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 40:		Line 41:
	[[Category: Rees, D C]]		[[Category: Rees, D C]]
	[[Category: Sherman, M A]]		[[Category: Sherman, M A]]
		+	[[Category: Acetylation]]
	[[Category: Glycolysis]]		[[Category: Glycolysis]]
	[[Category: Kinase]]		[[Category: Kinase]]

OCA at 10:53, 7 February 2016

OCA — Sun, 07 Feb 2016 10:53:28 GMT

←Older revision		Revision as of 10:53, 7 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qpg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:49, 10 September 2015

OCA — Thu, 10 Sep 2015 00:49:21 GMT

←Older revision		Revision as of 00:49, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1qpg' size='340' side='right' caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1qpg' size='340' side='right' caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QPG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QPG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [http://www.ebi.ac.uk/pdbsum/1qpg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [http://pdbe.org/1qpg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [http://www.ebi.ac.uk/pdbsum/1qpg PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1qpg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
	[[Category: Phosphoglycerate kinase]]		[[Category: Phosphoglycerate kinase]]
-	[[Category: Saccharomyces cerevisiae]]
	[[Category: Hsu, B T]]		[[Category: Hsu, B T]]
	[[Category: Mas, M T]]		[[Category: Mas, M T]]

OCA at 07:01, 6 January 2015

OCA — Tue, 06 Jan 2015 07:01:56 GMT

←Older revision		Revision as of 07:01, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QPG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QPG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [http://www.ebi.ac.uk/pdbsum/1qpg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [http://www.ebi.ac.uk/pdbsum/1qpg PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Phosphoglycerate kinase]]		[[Category: Phosphoglycerate kinase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Hsu, B T.]]	+	[[Category: Hsu, B T]]
-	[[Category: Mas, M T.]]	+	[[Category: Mas, M T]]
-	[[Category: Mcphillips, T M.]]	+	[[Category: Mcphillips, T M]]
-	[[Category: Rees, D C.]]	+	[[Category: Rees, D C]]
-	[[Category: Sherman, M A.]]	+	[[Category: Sherman, M A]]
	[[Category: Glycolysis]]		[[Category: Glycolysis]]
	[[Category: Kinase]]		[[Category: Kinase]]

OCA at 22:22, 28 September 2014

OCA — Sun, 28 Sep 2014 22:22:18 GMT

←Older revision		Revision as of 22:22, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1qpg.~~png\|left~~\|200px]]	+	==3-PHOSPHOGLYCERATE KINASE, MUTATION R65Q==
		+	<StructureSection load='1qpg' size='340' side='right' caption='[[1qpg]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1qpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QPG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene>, <scene name='pdbligand=MAP:MAGNESIUM-5-ADENYLY-IMIDO-TRIPHOSPHATE'>MAP</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qpg RCSB], [http://www.ebi.ac.uk/pdbsum/1qpg PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/1qpg_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structure of a ternary complex of the R65Q mutant of yeast 3-phosphoglycerate kinase (PGK) with magnesium 5'-adenylylimidodiphosphate (Mg-AMP-PNP) and 3-phospho-D-glycerate (3-PG) has been determined by X-ray crystallography to 2.4 angstrom resolution. The structure was solved by single isomorphous replacement, anamalous scattering, and solvent flattening and has been refined to an R-factor of 0.185, with rms deviations from ideal bond distance and angles of 0.009 angstrom and 1.78 degrees, respectively. PGK consists of two domains, with the 3-PG bound to a "basic patch" of residues from the N-terminal domain and the Mg-AMP-PNP interacting with residues from the C-terminal domain. The two ligands are separated by approximately 11 angstrom across the interdomain cleft. The model of the R65Q mutant of yeast PGK is very similar to the structures of PGK isolated from horse, pig, and Bacillus stearothermophilus (rms deviations between equivalent alpha-carbons in the individual domains < 1.0 angstrom) but exhibits substantial variations with a previously reported yeast structure (rms deviations between equivalent alpha-carbons in the individual domains of 2.9-3.2 angstrom). The most significant tertiary structural differences among the yeast R65Q, equine, porcine, and B. stearothermophilus PGK structures occur in the relative orientations of the two domains. However, the relationships between the observed conformations of PGK are inconsistent with a "hinge-bending" behavior that would close the interdomain cleft. It is proposed that the available structural and biochemical data on PGK may indicate that the basic patch primarily represents the site of anion activation and not the catalytically active binding site for 3-PG.

-	~~{{STRUCTURE_1qpg\| PDB=1qpg \| SCENE= }}~~	+	Structure of the R65Q mutant of yeast 3-phosphoglycerate kinase complexed with Mg-AMP-PNP and 3-phospho-D-glycerate.,McPhillips TM, Hsu BT, Sherman MA, Mas MT, Rees DC Biochemistry. 1996 Apr 2;35(13):4118-27. PMID:8672447<ref>PMID:8672447</ref>

-	~~===3-PHOSPHOGLYCERATE KINASE~~, ~~MUTATION R65Q===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8672447}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1qpg]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA].~~	+

	==See Also==		==See Also==
	*[[Phosphoglycerate Kinase\|Phosphoglycerate Kinase]]		*[[Phosphoglycerate Kinase\|Phosphoglycerate Kinase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:008672447<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Phosphoglycerate kinase]]		[[Category: Phosphoglycerate kinase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]

OCA at 06:25, 20 October 2012

OCA — Sat, 20 Oct 2012 06:25:07 GMT

←Older revision		Revision as of 06:25, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1qpg]] is a 1 chain structure ~~of [[Phosphoglycerate Kinase]]~~ with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA].	+	[[1qpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPG OCA].

	==See Also==		==See Also==

OCA: Protected "1qpg" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 13:07:49 GMT

Protected "1qpg" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:07, 25 July 2012