1quz - Revision history

OCA at 09:19, 6 December 2023

OCA — Wed, 06 Dec 2023 09:19:38 GMT

←Older revision		Revision as of 09:19, 6 December 2023
Line 1:		Line 1:

	==Solution structure of the potassium channel scorpion toxin HSTX1==		==Solution structure of the potassium channel scorpion toxin HSTX1==
-	<StructureSection load='1quz' size='340' side='right'caption='[[1quz~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1quz' size='340' side='right'caption='[[1quz]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Heterometrus_spinifer Heterometrus spinifer]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1quz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1quz OCA], [https://pdbe.org/1quz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1quz RCSB], [https://www.ebi.ac.uk/pdbsum/1quz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1quz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1quz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1quz OCA], [https://pdbe.org/1quz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1quz RCSB], [https://www.ebi.ac.uk/pdbsum/1quz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1quz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/KAX63_HETSP KAX63_HETSP]] Potently blocks voltage-gated potassium channels Kv1.1/KCNA1 and Kv1.3/KCNA3. Mildly blocks intermediate (IK) conductance calcium-activated potassium channels (KCa3.1/KCNN4) and ERG1/Kv11.1/KCNH2.<ref>PMID:15498765</ref> <ref>PMID:18687312</ref> <ref>PMID:9359871</ref>	+	[https://www.uniprot.org/uniprot/KAX63_HETSP KAX63_HETSP] Potently blocks voltage-gated potassium channels Kv1.1/KCNA1 and Kv1.3/KCNA3. Mildly blocks intermediate (IK) conductance calcium-activated potassium channels (KCa3.1/KCNN4) and ERG1/Kv11.1/KCNH2.<ref>PMID:15498765</ref> <ref>PMID:18687312</ref> <ref>PMID:9359871</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Heterometrus spinifer]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gilquin, B]]	+	[[Category: Gilquin B]]
-	[[Category: Lebrun, B]]	+	[[Category: Lebrun B]]
-	[[Category: Menez, A]]	+	[[Category: Menez A]]
-	[[Category: Nakajima, T]]	+	[[Category: Nakajima T]]
-	[[Category: Romi-Lebrun, R]]	+	[[Category: Romi-Lebrun R]]
-	[[Category: Savarin, P]]	+	[[Category: Savarin P]]
-	[[Category: Zinn-Justin, S]]	+	[[Category: Zinn-Justin S]]
-	~~[[Category: Alpha beta]]~~	+
-	~~[[Category: Molecular modeling]]~~	+
-	~~[[Category: Potassium channel]]~~	+
-	~~[[Category: Scorpion toxin]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 10:05, 15 September 2021

OCA — Wed, 15 Sep 2021 10:05:02 GMT

←Older revision		Revision as of 10:05, 15 September 2021
Line 3:		Line 3:
	<StructureSection load='1quz' size='340' side='right'caption='[[1quz]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1quz' size='340' side='right'caption='[[1quz]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1quz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1quz OCA], [~~http~~://pdbe.org/1quz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1quz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1quz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1quz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1quz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1quz OCA], [https://pdbe.org/1quz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1quz RCSB], [https://www.ebi.ac.uk/pdbsum/1quz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1quz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/KAX63_HETSP KAX63_HETSP]] Potently blocks voltage-gated potassium channels Kv1.1/KCNA1 and Kv1.3/KCNA3. Mildly blocks intermediate (IK) conductance calcium-activated potassium channels (KCa3.1/KCNN4) and ERG1/Kv11.1/KCNH2.<ref>PMID:15498765</ref> <ref>PMID:18687312</ref> <ref>PMID:9359871</ref>	+	[[https://www.uniprot.org/uniprot/KAX63_HETSP KAX63_HETSP]] Potently blocks voltage-gated potassium channels Kv1.1/KCNA1 and Kv1.3/KCNA3. Mildly blocks intermediate (IK) conductance calcium-activated potassium channels (KCa3.1/KCNN4) and ERG1/Kv11.1/KCNH2.<ref>PMID:15498765</ref> <ref>PMID:18687312</ref> <ref>PMID:9359871</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:33, 4 December 2019

OCA — Wed, 04 Dec 2019 11:33:21 GMT

←Older revision		Revision as of 11:33, 4 December 2019
Line 1:		Line 1:

	==Solution structure of the potassium channel scorpion toxin HSTX1==		==Solution structure of the potassium channel scorpion toxin HSTX1==
-	<StructureSection load='1quz' size='340' side='right' caption='[[1quz]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1quz' size='340' side='right'caption='[[1quz]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Potassium channel toxin\|Potassium channel toxin]]	+	*[[Potassium channel toxin 3D structures\|Potassium channel toxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gilquin, B]]		[[Category: Gilquin, B]]
	[[Category: Lebrun, B]]		[[Category: Lebrun, B]]

OCA at 07:58, 28 February 2018

OCA — Wed, 28 Feb 2018 07:58:19 GMT

←Older revision		Revision as of 07:58, 28 February 2018
Line 1:		Line 1:
		+
	==Solution structure of the potassium channel scorpion toxin HSTX1==		==Solution structure of the potassium channel scorpion toxin HSTX1==
	<StructureSection load='1quz' size='340' side='right' caption='[[1quz]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1quz' size='340' side='right' caption='[[1quz]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1quz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1quz OCA], [http://pdbe.org/1quz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1quz RCSB], [http://www.ebi.ac.uk/pdbsum/1quz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1quz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1quz OCA], [http://pdbe.org/1quz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1quz RCSB], [http://www.ebi.ac.uk/pdbsum/1quz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1quz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 23:30, 10 September 2015

OCA — Thu, 10 Sep 2015 23:30:58 GMT

←Older revision		Revision as of 23:30, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1quz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1quz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1quz RCSB], [http://www.ebi.ac.uk/pdbsum/1quz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1quz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1quz OCA], [http://pdbe.org/1quz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1quz RCSB], [http://www.ebi.ac.uk/pdbsum/1quz PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KAX63_HETSP KAX63_HETSP]] Potently blocks voltage-gated potassium channels Kv1.1/KCNA1 and Kv1.3/KCNA3. Mildly blocks intermediate (IK) conductance calcium-activated potassium channels (KCa3.1/KCNN4) and ERG1/Kv11.1/KCNH2.<ref>PMID:15498765</ref> <ref>PMID:18687312</ref> <ref>PMID:9359871</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1quz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 13:49, 17 December 2014

OCA — Wed, 17 Dec 2014 13:49:14 GMT

←Older revision		Revision as of 13:49, 17 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1quz~~\| ~~PDB~~=1quz \| ~~SCENE~~= }}	+	==Solution structure of the potassium channel scorpion toxin HSTX1==
-	===~~Solution~~ structure of the potassium channel scorpion toxin ~~HSTX1===~~	+	<StructureSection load='1quz' size='340' side='right' caption='[[1quz]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_10631983}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QUZ FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1quz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1quz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1quz RCSB], [http://www.ebi.ac.uk/pdbsum/1quz PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have determined the three-dimensional structure of the potassium channel inhibitor HsTX1, using nuclear magnetic resonance and molecular modeling. This protein belongs to the scorpion short toxin family, which essentially contains potassium channel blockers of 29 to 39 amino acids and three disulfide bridges. It is highly active on voltage-gated Kv1.3 potassium channels. Furthermore, it has the particularity to possess a fourth disulfide bridge. We show that HsTX1 has a fold similar to that of the three-disulfide-bridged toxins and conserves the hydrophobic core found in the scorpion short toxins. Thus, the fourth bridge has no influence on the global conformation of HsTX1. Most residues spatially analogous to those interacting with voltage-gated potassium channels in the three-disulfide-bridged toxins are conserved in HsTX1. Thus, we propose that Tyr21, Lys23, Met25, and Asn26 are involved in the biological activity of HsTX1. As an additional positively charged residue is always spatially close to the aromatic residue in toxins blocking the voltage-gated potassium channels, and as previous mutagenesis experiments have shown the critical role played by the C-terminus in HsTX1, we suggest that Arg33 is also important for the activity of the four disulfide-bridged toxin. Docking calculations confirm that, if Lys23 and Met25 interact with the GYGDMH motif of Kv1.3, Arg33 can contact Asp386 and, thus, play the role of the additional positively charged residue of the toxin functional site. This original configuration of the binding site of HsTX1 for Kv1.3, if confirmed experimentally, offers new structural possibilities for the construction of a molecule blocking the voltage-gated potassium channels.

-	~~==About this Structure==~~	+	Structural and functional consequences of the presence of a fourth disulfide bridge in the scorpion short toxins: solution structure of the potassium channel inhibitor HsTX1.,Savarin P, Romi-Lebrun R, Zinn-Justin S, Lebrun B, Nakajima T, Gilquin B, Menez A Protein Sci. 1999 Dec;8(12):2672-85. PMID:10631983<ref>PMID:10631983</ref>
-	~~[[1quz]] is~~ a ~~1 chain~~ structure. ~~Full experimental information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1QUZ OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Potassium channel toxin\|Potassium channel toxin]]		*[[Potassium channel toxin\|Potassium channel toxin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010631983<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
-	[[Category: Gilquin, B.]]	+	</StructureSection>
-	[[Category: Lebrun, B.]]	+	[[Category: Gilquin, B]]
-	[[Category: Menez, A.]]	+	[[Category: Lebrun, B]]
-	[[Category: Nakajima, T.]]	+	[[Category: Menez, A]]
-	[[Category: Romi-Lebrun, R.]]	+	[[Category: Nakajima, T]]
-	[[Category: Savarin, P.]]	+	[[Category: Romi-Lebrun, R]]
-	[[Category: Zinn-Justin, S.]]	+	[[Category: Savarin, P]]
		+	[[Category: Zinn-Justin, S]]
	[[Category: Alpha beta]]		[[Category: Alpha beta]]
	[[Category: Molecular modeling]]		[[Category: Molecular modeling]]

OCA at 09:58, 11 March 2013

OCA — Mon, 11 Mar 2013 09:58:58 GMT

←Older revision		Revision as of 09:58, 11 March 2013
Line 1:		Line 1:
-	[[Image:1quz.png\|left\|200px]]
-
	{{STRUCTURE_1quz\| PDB=1quz \| SCENE= }}		{{STRUCTURE_1quz\| PDB=1quz \| SCENE= }}
-
	===Solution structure of the potassium channel scorpion toxin HSTX1===		===Solution structure of the potassium channel scorpion toxin HSTX1===
-
	{{ABSTRACT_PUBMED_10631983}}		{{ABSTRACT_PUBMED_10631983}}

	==About this Structure==		==About this Structure==
	[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA].		[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA].
		+
		+	==See Also==
		+	*[[Potassium channel toxin\|Potassium channel toxin]]

	==Reference==		==Reference==

OCA: Protected "1quz" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 18:35:10 GMT

Protected "1quz" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:35, 14 August 2012

OCA at 18:35, 14 August 2012

OCA — Tue, 14 Aug 2012 18:35:08 GMT

←Older revision		Revision as of 18:35, 14 August 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1quz.png\|left\|200px]]		[[Image:1quz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1quz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1quz\| PDB=1quz \| SCENE= }}		{{STRUCTURE_1quz\| PDB=1quz \| SCENE= }}

	===Solution structure of the potassium channel scorpion toxin HSTX1===		===Solution structure of the potassium channel scorpion toxin HSTX1===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10631983}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10631983 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10631983}}		{{ABSTRACT_PUBMED_10631983}}

	==About this Structure==		==About this Structure==
-	~~1QUZ is a~~ [[~~Single protein~~]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA].	+	[[1quz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA].

	==Reference==		==Reference==
-	Structural and functional consequences of the presence of a fourth disulfide bridge in the scorpion short toxins: solution structure of the potassium channel inhibitor HsTX1., Savarin P, Romi-Lebrun R, Zinn-Justin S, Lebrun B, Nakajima T, Gilquin B, Menez A, Protein Sci. 1999 Dec;8(12):2672-85. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/10631983 10631983]~~	+	<ref group="xtra">PMID:010631983</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Gilquin, B.]]		[[Category: Gilquin, B.]]
	[[Category: Lebrun, B.]]		[[Category: Lebrun, B.]]
Line 34:		Line 21:
	[[Category: Alpha beta]]		[[Category: Alpha beta]]
	[[Category: Molecular modeling]]		[[Category: Molecular modeling]]
-	[[Category: Nmr]]
	[[Category: Potassium channel]]		[[Category: Potassium channel]]
	[[Category: Scorpion toxin]]		[[Category: Scorpion toxin]]
-		+	[[Category: Toxin]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Jul 28 14:43:42 2008''~~	+

OCA at 11:43, 28 July 2008

OCA — Mon, 28 Jul 2008 11:43:47 GMT

←Older revision		Revision as of 11:43, 28 July 2008
Line 1:		Line 1:
-	[[Image:1quz.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1quz.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1quz\| PDB=1quz \| SCENE= }}		{{STRUCTURE_1quz\| PDB=1quz \| SCENE= }}

-	~~'''~~Solution structure of the potassium channel scorpion toxin HSTX1~~'''~~	+	===Solution structure of the potassium channel scorpion toxin HSTX1===


-	~~==Overview==~~	+	<!--
-	~~We have determined the three-dimensional structure of the potassium channel inhibitor HsTX1~~, ~~using nuclear magnetic resonance and molecular modeling. This protein belongs to the scorpion short toxin family~~, ~~which essentially contains potassium channel blockers of 29 to 39 amino acids and three disulfide bridges. It is highly active on voltage-gated Kv1.3 potassium channels. Furthermore, it has~~ the ~~particularity~~ to ~~possess a fourth disulfide bridge. We show that HsTX1 has a fold similar to that of~~ the ~~three-disulfide-bridged toxins and conserves the hydrophobic core found in the scorpion short toxins. Thus, the fourth bridge has no influence~~ on ~~the global conformation of HsTX1~~. ~~Most residues spatially analogous to those interacting with voltage-gated potassium channels in the three-disulfide-bridged toxins are conserved in HsTX1~~. ~~Thus~~, ~~we propose that Tyr21, Lys23, Met25, and Asn26 are involved in~~ the ~~biological activity of HsTX1~~. ~~As an additional positively charged residue is always spatially close to the aromatic residue in toxins blocking the voltage~~-~~gated potassium channels, and as previous mutagenesis experiments have shown the critical role played by the C~~-terminus in HsTX1, we suggest that Arg33 is also important for the activity of the four disulfide-bridged toxin. Docking calculations confirm that, if Lys23 and Met25 interact with the GYGDMH motif of Kv1.3, Arg33 can contact Asp386 and, thus, play the role of the additional positively charged residue of the toxin functional site. This original configuration of the binding site of HsTX1 for Kv1.3, if confirmed experimentally, offers new structural possibilities for the construction of a molecule blocking the voltage-gated potassium channels.	+	The line below this paragraph, {{ABSTRACT_PUBMED_10631983}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 10631983 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_10631983}}

	==About this Structure==		==About this Structure==
-	1QUZ is a [[Single protein]] structure. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA].	+	1QUZ is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA].

	==Reference==		==Reference==
Line 33:		Line 37:
	[[Category: Potassium channel]]		[[Category: Potassium channel]]
	[[Category: Scorpion toxin]]		[[Category: Scorpion toxin]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 06~~:44:04 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:43:42 2008''